LOCUS       NM_053628              11924 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Rattus norvegicus multiple EGF-like-domains 8 (Megf8), mRNA.
ACCESSION   NM_053628 XM_001077319 XM_341803
VERSION     NM_053628.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Yuan,X., Chen,R., Luo,G., Sun,P., Song,X., Ma,J., Sun,R., Yu,T. and
            Jiang,Z.
  TITLE     Role and mechanism of miR-871-3p/Megf8 in regulating
            formaldehyde-induced cardiomyocyte inflammation and congenital
            heart disease
  JOURNAL   Int Immunopharmacol 126, 111297 (2024)
   PUBMED   38039718
  REMARK    GeneRIF: Role and mechanism of miR-871-3p/Megf8 in regulating
            formaldehyde-induced cardiomyocyte inflammation and congenital
            heart disease.
REFERENCE   2  (bases 1 to 11924)
  AUTHORS   Pusapati,G.V., Kong,J.H., Patel,B.B., Krishnan,A., Sagner,A.,
            Kinnebrew,M., Briscoe,J., Aravind,L. and Rohatgi,R.
  TITLE     CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity
            to the Morphogen Sonic Hedgehog
  JOURNAL   Dev Cell 44 (1), 113-129 (2018)
   PUBMED   29290584
  REMARK    Erratum:[Dev Cell. 2018 Jan 22;44(2):271. doi:
            10.1016/j.devcel.2018.01.002.. PMID: 29401421]
REFERENCE   3  (bases 1 to 11924)
  AUTHORS   Li,Y., Klena,N.T., Gabriel,G.C., Liu,X., Kim,A.J., Lemke,K.,
            Chen,Y., Chatterjee,B., Devine,W., Damerla,R.R., Chang,C., Yagi,H.,
            San Agustin,J.T., Thahir,M., Anderton,S., Lawhead,C., Vescovi,A.,
            Pratt,H., Morgan,J., Haynes,L., Smith,C.L., Eppig,J.T.,
            Reinholdt,L., Francis,R., Leatherbury,L., Ganapathiraju,M.K.,
            Tobita,K., Pazour,G.J. and Lo,C.W.
  TITLE     Global genetic analysis in mice unveils central role for cilia in
            congenital heart disease
  JOURNAL   Nature 521 (7553), 520-524 (2015)
   PUBMED   25807483
REFERENCE   4  (bases 1 to 11924)
  AUTHORS   Engelhard,C., Sarsfield,S., Merte,J., Wang,Q., Li,P., Beppu,H.,
            Kolodkin,A.L., Sucov,H.M. and Ginty,D.D.
  TITLE     MEGF8 is a modifier of BMP signaling in trigeminal sensory neurons
  JOURNAL   Elife 2, e01160 (2013)
   PUBMED   24052814
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 11924)
  AUTHORS   Twigg,S.R., Lloyd,D., Jenkins,D., Elcioglu,N.E., Cooper,C.D.,
            Al-Sannaa,N., Annagur,A., Gillessen-Kaesbach,G., Huning,I.,
            Knight,S.J., Goodship,J.A., Keavney,B.D., Beales,P.L., Gileadi,O.,
            McGowan,S.J. and Wilkie,A.O.
  TITLE     Mutations in multidomain protein MEGF8 identify a Carpenter
            syndrome subtype associated with defective lateralization
  JOURNAL   Am J Hum Genet 91 (5), 897-905 (2012)
   PUBMED   23063620
REFERENCE   6  (bases 1 to 11924)
  AUTHORS   Zhang,Z., Alpert,D., Francis,R., Chatterjee,B., Yu,Q., Tansey,T.,
            Sabol,S.L., Cui,C., Bai,Y., Koriabine,M., Yoshinaga,Y., Cheng,J.F.,
            Chen,F., Martin,J., Schackwitz,W., Gunn,T.M., Kramer,K.L., De
            Jong,P.J., Pennacchio,L.A. and Lo,C.W.
  TITLE     Massively parallel sequencing identifies the gene Megf8 with
            ENU-induced mutation causing heterotaxy
  JOURNAL   Proc Natl Acad Sci U S A 106 (9), 3219-3224 (2009)
   PUBMED   19218456
REFERENCE   7  (bases 1 to 11924)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   8  (bases 1 to 11924)
  AUTHORS   Aune,C.N., Chatterjee,B., Zhao,X.Q., Francis,R., Bracero,L., Yu,Q.,
            Rosenthal,J., Leatherbury,L. and Lo,C.W.
  TITLE     Mouse model of heterotaxy with single ventricle spectrum of cardiac
            anomalies
  JOURNAL   Pediatr Res 63 (1), 9-14 (2008)
   PUBMED   18043505
REFERENCE   9  (bases 1 to 11924)
  AUTHORS   Nakayama,M., Nakajima,D., Nagase,T., Nomura,N., Seki,N. and
            Ohara,O.
  TITLE     Identification of high-molecular-weight proteins with multiple
            EGF-like motifs by motif-trap screening
  JOURNAL   Genomics 51 (1), 27-34 (1998)
   PUBMED   9693030
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Sep 11, 2014 this sequence version replaced NM_053628.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments and orthologous data.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760383,
                              SAMEA5760386 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-530               JAXUCZ010000001.1  90030388-90030917
            531-694             JAXUCZ010000001.1  90038909-90039072
            695-901             JAXUCZ010000001.1  90039885-90040091
            902-1082            JAXUCZ010000001.1  90040915-90041095
            1083-1171           JAXUCZ010000001.1  90041182-90041270
            1172-1587           JAXUCZ010000001.1  90042274-90042689
            1588-1733           JAXUCZ010000001.1  90043111-90043256
            1734-1856           JAXUCZ010000001.1  90043439-90043561
            1857-2011           JAXUCZ010000001.1  90046034-90046188
            2012-2131           JAXUCZ010000001.1  90046428-90046547
            2132-2276           JAXUCZ010000001.1  90046878-90047022
            2277-2440           JAXUCZ010000001.1  90047096-90047259
            2441-2641           JAXUCZ010000001.1  90049946-90050146
            2642-2842           JAXUCZ010000001.1  90050512-90050712
            2843-3079           JAXUCZ010000001.1  90051519-90051755
            3080-3198           JAXUCZ010000001.1  90052575-90052693
            3199-3330           JAXUCZ010000001.1  90052773-90052904
            3331-3444           JAXUCZ010000001.1  90052991-90053104
            3445-3693           JAXUCZ010000001.1  90053329-90053577
            3694-3893           JAXUCZ010000001.1  90054053-90054252
            3894-4104           JAXUCZ010000001.1  90054555-90054765
            4105-4354           JAXUCZ010000001.1  90054850-90055099
            4355-4487           JAXUCZ010000001.1  90055821-90055953
            4488-4735           JAXUCZ010000001.1  90058293-90058540
            4736-4846           JAXUCZ010000001.1  90058620-90058730
            4847-4965           JAXUCZ010000001.1  90058860-90058978
            4966-5173           JAXUCZ010000001.1  90059545-90059752
            5174-5354           JAXUCZ010000001.1  90060948-90061128
            5355-5518           JAXUCZ010000001.1  90061652-90061815
            5519-5663           JAXUCZ010000001.1  90062398-90062542
            5664-5895           JAXUCZ010000001.1  90064395-90064626
            5896-6019           JAXUCZ010000001.1  90065516-90065639
            6020-6233           JAXUCZ010000001.1  90065794-90066007
            6234-6448           JAXUCZ010000001.1  90066214-90066428
            6449-6656           JAXUCZ010000001.1  90069722-90069929
            6657-6816           JAXUCZ010000001.1  90070093-90070252
            6817-7009           JAXUCZ010000001.1  90070596-90070788
            7010-7180           JAXUCZ010000001.1  90071292-90071462
            7181-7311           JAXUCZ010000001.1  90071917-90072047
            7312-7444           JAXUCZ010000001.1  90072459-90072591
            7445-11924          JAXUCZ010000001.1  90074943-90079422
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q21"
     gene            1..11924
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /note="multiple EGF-like-domains 8"
                     /db_xref="GeneID:114029"
                     /db_xref="RGD:621190"
     exon            1..530
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     CDS             344..8713
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /note="multiple epidermal growth factor-like domains
                     protein 8; multiple EGF-like domain protein 4; EGF-like
                     domain-containing protein 4; multiple EGF-like domains
                     protein 8; epidermal growth factor-like protein 4"
                     /codon_start=1
                     /product="multiple epidermal growth factor-like domains
                     protein 8 precursor"
                     /protein_id="NP_446080.1"
                     /db_xref="GeneID:114029"
                     /db_xref="RGD:621190"
                     /translation="MALGGALAAALALAFAVLGPLSHKVLAGDCKGQRQVLREAPGFV
                     TDGAGNYSVNGNCEWLIEAPSPQHRILLDFLFLDTECTYDYLFVYDGDSPQGPLLASL
                     SGSTRPPPIEASSGKMLLHLFSDANYNLLGFNASFRFSLCPGGCQNHGQCKSPGVCVC
                     EPGWGGPDCGLQECSAYCGSHGTCASTLGPCRCEPGFLGRACDLHLWENQGAGWWHSV
                     SAGDPAFSARVGAAGAFLSPPGLLAVFGGQDLNKALGDLVLYNFSTNTWESWDLTPAP
                     AARHSHVAVAWAGFLVLMGGELANGLLTNDVWAFSPLGGGHWELLAPPASSSSGPPGL
                     AGHAAALVDDIWLYVSGGRTQHDLFSSGLFRFRLDHTSRGYWEQVIPAGGRPPAATGH
                     SMVFHAPSRTLLVHGGHRPSTARFSVRVNSTELFHVDRRVWTTLKGRDGLQGPRERAF
                     HTASVLGNYMVVYGGNVHTHYQEEKCYEDGIFFYHLGCHQWVSGAELAPPGTPEGRAA
                     PPSGRYSHVAAVLGGSVLLVAGGYSGRPRGDLMAYKVPPFVFQAPALDYHLDYCSMYT
                     DHSVCSRDPECSWCQGACQSAPPPGTPSGACPAASCLGLGRLLSDCQACLAFSSPTAP
                     PRGPGTLGWCVHNESCLPRPEQARCRGEQISGTVGWWGPAPVFVTSLEACVTQSFLPG
                     LHLLTFQQPPNASQPDKVSIVRSTTITLTPSAETDVSLVYRGFIYPMLPGGPGGPGAE
                     DVAVWARAQRLHVLARMARGPDTENMEEVGRWVAQQEKETRRLQRPGSSRLFPLPGRG
                     NKYAVEIRGQLNGSAGPGHSELTLLWDRTGVPGGSEISFFFLEPYRSLACSSYSSCLG
                     CLADQGCGWCLNSATCHLRQGRAHCEDDGNGESLLVLVPALCPLCEEHRDCHACTQDP
                     FCEWHQSTNRKGDAACSRRGRGRGALKNPEECPPLCSQRLTCEDCLANSSQCAWCQST
                     HTCFLFAAYLARYPHGGCRGWDDSVHSEPRCRSCHGFLTCHECLQSHECGWCGNEDNP
                     TLGRCLQGDFSGPLGGGNCSLWVGEGLGLPVALPARWAYARCPDVDECRLGLARCHPR
                     ATCLNTPLSYECHCQRGYQGDGITHCNRTCLEDCGHGVCSGPPDFTCVCDLGWTSDLP
                     PPTPAPGPPAPRCSRDCGCNFHSHCRRRGPGYCDECQDWTWGEHCERCRPGSFGNATG
                     SGGCRPCQCNGHGDPRRGHCDNLSGLCFCQDHTEGAHCQICSPGYYGDPRAGGSCFRE
                     CGGRALLTNVSSVALGSRRFGGLLPPGGGTARAGPGLSYCVWVVSATEALQPCAPGTL
                     CPPLTLTFSPDSSTPCTLSYVLAFDGFPRFLDTGVVQSDRSLIAAFCGQRRDRPLTVQ
                     ALSGLLVLHWEANGSSSWGFNASVGSARCGSGGPGSCPVPQECVPQDGAAGAGLCRCP
                     QGWAGPHCRMALCPENCNAHTGAGICNQSLGVCICAEGFGGPDCATKLDGGQLVWETL
                     MDSRLSADTASRFLHRLGHTMVEGPDATLWMFGGLGLPQGLLGNLYRYSVSERRWTQM
                     LAGAEDGGPGPSPRSFHAAAYVPAGRGAMYLLGGLTAGGITCDFWVLNLTTLQWRQEK
                     APQSIELPAVAGHTLTARRGLSLLLVGGYSPENGFNQQLLEYQLATGTWVSGAQSGTP
                     PTGLYGHSAVYHEATDSLYVFGGFRFHVELAAPSPELYSLHCPDRTWSLLAPSQGAKP
                     RPRLFHASALLGDTMVVLGGRSDPDEFSSDVLLYQVNCNTWLLPDLTRPAFVGSPMEE
                     SVAHAVAAVGSRLYISGGFGGVALGRLLALTLPPDPCRLLPSPEACNQSGACTWCHGA
                     CLSGDQAHRLGCGVPPCSPMPRSPEECRRLRTCSECLARHPRTLQPGDGEASVPRCKW
                     CTNCPEGACIGRNGSCTSENDCRINQREVFWAGNCSEAACGAADCEQCTREGKCMWTR
                     QFKRTGETRRILSVQPTYDWTCFSHSLLNVSPMPVESSPPLPCPTPCHLLPNCTSCLA
                     SKGADGGWQHCVWSSSLQQCLSPSYLPLRCMAGGCGRLLRGPESCSLGCAQATQCALC
                     LRRPHCGWCAWGGQDGGGHCMEGGLSGPRDGLTCGRPGASWAFLSCPPEDECANGHHD
                     CNETQNCHDQPHGYECSCKTGYTMDNVTGVCRPVCAQGCVNGSCVEPDHCRCHFGFVG
                     RNCSTECRCNRHSECAGVGARDHCLLCRNHTKGSHCEQCLPLFVGSALGGGTCRPCHA
                     FCRGNSHVCVSRKELEMARREPEKYSLDPEEIEAWVAEGPSEDEAVCVNCQNNSYGDR
                     CESCLHGYFLLDGKCTKCQCNGHADTCNEQDGTGCPCQNNTETGVCQGSSPSDRRDCY
                     KYQCAKCRESFHGSPLGGQQCYRLISVEQECCLDPTSQTNCFHEPKRRALGPGRTVLF
                     GVQPKFTNVDIRLTLDVTFGAVDLYVSTSYDTFVVRVAPDTGVHTVHIQPPPPPPPPP
                     PPADGVPRVASDLGGLGTGSGSGSPVEPRVREVWPRGLITYVTVTEPSAVLVVRSVRD
                     RLVITYPHEHHALKSSRFYLLLLGVGDPNGPGANGSADSQGLLFFRQDQAHIDLFVFF
                     SVFFSCFFLFLSLCVLLWKAKQALDQRQEQRRHLQEMTKMASRPFAKVTVCFPPDPAG
                     PAPAWKPAGLPPPAFRRSEPFLAPLLLTGAGGPWGPMGGGCCPPALPATTAGLRAGPI
                     TLEPTEDGMAGVATLLLQLPGGPHAPNGACLGSALVTLRHRLHEYCGGSGGAGGSGHG
                     GGGGRKGLLSQDNLTSMSL"
     exon            531..694
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            695..901
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1082
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1171
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1587
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1733
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..1856
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            1857..2011
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            2012..2131
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            2132..2276
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..2440
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            2441..2641
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            2642..2842
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            2843..3079
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            3080..3198
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            3199..3330
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            3331..3444
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            3445..3693
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            3694..3893
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            3894..4104
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            4105..4354
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            4355..4487
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            4488..4735
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            4736..4846
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            4847..4965
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            4966..5173
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            5174..5354
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            5355..5518
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            5519..5663
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            5664..5895
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            5896..6019
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            6020..6233
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            6234..6448
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            6449..6656
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            6657..6816
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            6817..7009
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            7010..7180
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            7181..7311
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            7312..7444
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
     exon            7445..11924
                     /gene="Megf8"
                     /gene_synonym="Egfl4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cttgcagcag cctgagtcta atcattagac accgtttatg gggtcggcgc ccctatgggg
       61 gctcgtgtct ataggggacc cttacggtcc ctaggggtca gccctatcca taacgacttc
      121 atatccaggg ccttccatgg gtccataggg ccggcctctc ggtttcaaaa acccatcaac
      181 agcggccctt tccttggccg ggactgcgtc gctggtacct ccttggacta caaggccatg
      241 ctacgcctgt aaaggttgca tctgcagggc ctcattcccg tagagtgtcc attcctggtc
      301 ttcagggcct tcccgtctct taggaccaga gccaaaggcg gcgatggcct tgggcggagc
      361 tctggccgcg gcccttgcac tggccttcgc tgtgctgggg ccgctgtccc acaaggtcct
      421 ggccggtgac tgcaaggggc agcggcaggt gctacgggag gcaccaggct tcgtgacgga
      481 tggtgcgggc aactacagcg tcaatggcaa ctgcgagtgg cttatcgagg caccgagccc
      541 tcagcaccgg atcctgctgg acttcctttt cctggacaca gagtgcactt atgactacct
      601 ctttgtgtat gatggtgact caccccaagg cccgctgctt gctagtctga gtgggagcac
      661 ccggccccca cccatcgagg catcctcagg caagatgctg ctccatctct tcagtgatgc
      721 caactacaac ctgctgggct ttaacgcctc cttccgcttc tccctgtgtc ctggaggttg
      781 ccagaaccac gggcagtgca agtctccagg agtgtgtgtc tgcgagccag gctggggggg
      841 tccagactgt ggcctgcagg agtgctcagc ctactgtggt agccacggta cctgtgcctc
      901 gaccctggga ccatgccgct gtgagcctgg attcctggga cgggcctgtg acctgcactt
      961 gtgggagaac cagggggctg gctggtggca cagtgtgagt gccggggacc ccgccttctc
     1021 tgcccgagtt ggggcagctg gtgccttcct gtctccacca gggctgctgg ctgtctttgg
     1081 aggtcaggac cttaacaagg ccctggggga tcttgtcctg tacaacttct ccaccaacac
     1141 ctgggaatcc tgggatctga cgcctgctcc ggccgcccgt cactcccacg tggccgtggc
     1201 ctgggccggc ttcctggtgc tgatgggtgg tgagctggcc aatggcttgc taaccaacga
     1261 tgtgtgggcc tttagccccc tgggtggagg ccactgggag ctcctggcac cacctgcttc
     1321 cagttcctct gggcccccag ggctggcagg ccatgcagca gctctggtag atgacatctg
     1381 gctgtatgtg tccggtggcc gcactcagca tgacctcttt tcttctggcc tcttccgctt
     1441 ccgcctggac cacaccagca ggggctactg ggagcaggtg atccctgcag gtgggcggcc
     1501 ccctgctgcc actggccact ctatggtgtt ccacgccccg tcccgtaccc tgctggtcca
     1561 tggtggacac cggccttcca ctgcccggtt ttccgtgcgg gtgaattcca ccgagctctt
     1621 ccacgtggac cgccgtgtgt ggaccacgct gaagggccgg gatgggctcc agggtccacg
     1681 ggagagagcc ttccacacag ccagtgttct aggaaactac atggtggtct atgggggcaa
     1741 tgtgcacacc cattaccagg aggaaaagtg ttacgaagat ggcatcttct tctaccacct
     1801 tggttgccac caatgggtgt cgggggccga acttgcccca ccagggaccc ctgaaggtcg
     1861 agcagcgccc cctagtggtc gttactcaca tgtggcagct gttctcggtg gcagtgtcct
     1921 gctggtggct gggggataca gtggccggcc tcgtggggac ttgatggcct acaaggtgcc
     1981 ccccttcgtg tttcaggcac ccgccctgga ttaccacttg gactactgtt ccatgtacac
     2041 agaccacagt gtgtgctccc gggaccccga atgtagctgg tgtcagggtg cttgccaatc
     2101 cgcaccccct cccgggactc cctcaggagc ttgtcccgca gccagctgcc tgggcctagg
     2161 ccgcctactg agtgactgcc aggcatgcct ggccttcagc agccccacgg ctcctccccg
     2221 aggacctggc accctaggtt ggtgtgtgca caacgagagc tgccttcccc ggccagagca
     2281 ggcacgctgc cgaggggagc agatctcagg cactgtgggc tggtgggggc cagcacccgt
     2341 ctttgtcact tccctggagg cttgtgtcac ccagagcttc ctccctggcc tgcacctgct
     2401 caccttccag cagccaccca acgcctctca gcctgacaag gtctccattg tccgaagtac
     2461 aactatcacc ttgaccccca gcgcggagac agatgtgtcc ctggtctacc gaggcttcat
     2521 ctacccgatg ctgccaggag gaccaggtgg ccctggggcc gaagatgtgg ctgtgtgggc
     2581 tcgggcccag cgcctccatg tcctggcccg gatggccaga ggccctgaca ctgagaacat
     2641 ggaagaggtg ggacgctggg tggctcagca ggagaaggag acacggcgtc tgcagcgccc
     2701 cggctctagt cgcctcttcc cactgcccgg tcgaggcaac aagtatgctg tggagatccg
     2761 aggccagctc aatggctccg caggccctgg gcacagtgag ctcactctcc tgtgggatcg
     2821 gactggagtg ccgggtggca gtgagatctc cttcttcttc ctggagccct accgctcttt
     2881 ggcctgctcc tcctactcct cctgtctggg ctgcctggcc gatcagggct gtggctggtg
     2941 cctcaacagt gccacctgtc acctgcgtca gggcagggcc cactgtgagg atgacgggaa
     3001 cggcgagtcc ttgctggtgc tggtgcctgc cctctgtcca ctctgtgagg agcatcgtga
     3061 ctgccatgcc tgcacccagg accccttttg tgagtggcat cagagcacca accgcaaagg
     3121 tgacgcagcc tgcagccggc gaggccgggg ccgaggcgct ctgaagaatc ccgaggaatg
     3181 ccccccactc tgcagccaac gcctgacctg tgaggactgc ctggccaact ctagccagtg
     3241 cgcctggtgc cagtcaactc acacctgctt cctgtttgct gcctacctgg cccggtaccc
     3301 acacgggggc tgccgtggct gggacgacag tgtgcattca gagccacgct gcaggagctg
     3361 ccacggattt ttgacttgtc acgagtgtct acagagccac gagtgtggtt ggtgtggcaa
     3421 tgaagacaac cccactctgg gacggtgcct acagggggac ttctccggac ccctgggtgg
     3481 ggggaactgc tccctgtggg tgggagaggg cctggggctg cctgtggctc ttcctgctcg
     3541 ctgggcttat gcccgctgtc cagatgtgga tgaatgtcgc ctgggtctgg cgaggtgtca
     3601 cccacgggca acgtgcctca acacgcctct cagctatgag tgtcattgcc agcgcggcta
     3661 ccagggtgat ggcatcacac actgtaaccg cacgtgcctg gaagactgtg gccacggtgt
     3721 gtgtagcggt cccccggact tcacttgcgt gtgtgacctg ggttggacct cagatttgcc
     3781 accacccaca cctgccccag gaccccctgc cccacgctgc tctcgagact gtggctgcaa
     3841 ctttcacagt cattgccgcc ggcggggacc tggctactgt gatgagtgcc aggactggac
     3901 atggggggaa cactgtgaac ggtgccggcc tggcagcttt ggcaatgcaa cgggctctgg
     3961 tggctgccga ccctgccagt gtaacgggca tggagaccca cgtcgtggcc actgtgacaa
     4021 cctcagtggg ctctgcttct gccaggacca tactgaaggt gcccactgtc agatttgctc
     4081 tccaggatac tatggggacc cccgagctgg tggctcctgc ttccgggagt gtgggggtcg
     4141 tgccctcctc accaatgtgt cctcagtggc tctgggctct cgccgctttg ggggacttct
     4201 gcctccaggt ggtgggacag caagagctgg acccggcctg tcctactgtg tgtgggttgt
     4261 ctcagccaca gaagcgctgc agccctgtgc ccctgggacc ctctgtcccc cactcaccct
     4321 caccttctct ccagacagca gcaccccttg cacactgagc tatgtcctgg cctttgatgg
     4381 attcccacgc tttctggaca ctggggtcgt ccagtcagac cggagcctca tagctgcctt
     4441 ctgtggtcag cggcgggaca gacctctcac cgtgcaggct ttatctgggt tacttgtgtt
     4501 gcactgggag gccaatggct cctcctcctg gggattcaat gcttccgtgg gctctgcccg
     4561 atgtggatca ggaggccctg gcagttgccc tgtccctcag gagtgtgtgc cccaggatgg
     4621 ggctgcaggt gctgggctct gccgatgtcc tcagggctgg gctgggcctc actgccgcat
     4681 ggctctgtgt cctgagaact gcaacgccca cacgggggca gggatttgta accagagcct
     4741 aggtgtgtgc atctgtgctg agggctttgg tggcccagac tgtgccacga agctggatgg
     4801 tgggcagttg gtctgggaaa cactcatgga cagccgcctc tcagctgaca ctgctagtcg
     4861 cttccttcac cgcctggggc atactatggt agagggacct gatgctactt tgtggatgtt
     4921 tgggggactg ggtctgccgc agggcctgct gggaaacttg tataggtatt cagtcagtga
     4981 gcgcaggtgg acacagatgc tggcaggagc tgaggatggg ggcccaggcc catcaccccg
     5041 ctcctttcac gcagctgcct atgtacctgc tggccgtggt gccatgtacc tgctgggggg
     5101 cctcacagct gggggcatca cctgtgactt ctgggtcctt aacctcacta ccctacagtg
     5161 gagacaggag aaggcccctc agagcataga gctgccagca gtcgctggtc acacccttac
     5221 tgcccgtcga ggcctgtctc tgctcttggt gggtggctac tcccctgaaa atggcttcaa
     5281 ccagcagttg ttggagtacc agctggcaac cggcacgtgg gtgtctggag cccagagtgg
     5341 gacgccccca acaggtctct atggccattc tgcggtctac catgaggcca ccgactccct
     5401 ctatgtgttt gggggtttcc gcttccatgt ggaattggcg gcgccttctc cggagctcta
     5461 ctccctgcac tgtcctgacc gcacctggag cttgctggca ccttctcagg gggccaagcc
     5521 ccggccccgg ctcttccatg cctcagccct gctgggggac accatggtgg tcctcggggg
     5581 gcgctcggac cccgatgaat tcagcagtga tgtcctgctc taccaggtca actgcaacac
     5641 ctggctcctg cctgacctca cccgtccagc ctttgtgggg tccccgatgg aggagtccgt
     5701 ggctcacgca gtggcagcag tgggtagccg cctgtacatc tctggaggat ttgggggagt
     5761 agccttgggc cgcctgctgg cattaaccct gccccctgac ccctgtcgtc tgctgccttc
     5821 acctgaagct tgcaaccagt ctggggcctg cacctggtgc catggtgctt gcctgtctgg
     5881 ggaccaggcc cacagactgg gttgtggggt gcccccttgc tccccaatgc ctcgctcgcc
     5941 tgaggaatgt cggagactgc ggacctgcag tgagtgcctg gcccgccatc ctcggaccct
     6001 gcagcctgga gatggagagg catccgttcc ccgctgtaag tggtgcacta actgcccaga
     6061 gggcgcctgc atcgggcgca atgggtcctg cacctccgag aatgactgtc gaatcaacca
     6121 acgagaggtc ttctgggctg gtaactgctc agaggccgca tgcggggctg ccgactgtga
     6181 gcagtgcaca cgggagggaa agtgcatgtg gactcggcaa ttcaagagga ctggggagac
     6241 tcggcgtatc ctgtcagtgc agcccaccta cgactggact tgcttcagcc attctctgct
     6301 gaatgtgtct cctatgccgg tggagtcctc acccccactg ccctgtccca ccccctgtca
     6361 cctcctcccc aattgcacat cctgcctggc ctccaagggt gcagatggag gctggcagca
     6421 ctgtgtgtgg agcagcagcc tccagcagtg tctgagcccc tcctacctgc ccttacgctg
     6481 tatggctgga ggctgtgggc ggctgcttcg ggggccagag agctgctccc tgggctgtgc
     6541 tcaggctacg cagtgcgccc tgtgtctgcg aagaccccac tgtggctggt gtgcctgggg
     6601 gggacaggat ggaggtggcc actgcatgga aggtggtctc agtggccccc gagatgggct
     6661 cacgtgtgga cgtcctgggg catcctgggc cttcctgtcc tgcccccctg aggacgagtg
     6721 tgcaaacggg caccatgact gcaatgagac tcagaactgc cacgaccagc cccacggcta
     6781 cgagtgcagc tgcaagacag gctacaccat ggacaatgtg actggcgtct gccgccctgt
     6841 gtgtgcccag ggctgtgtga acggctcctg tgtggagcct gatcactgcc ggtgtcactt
     6901 tggctttgtt ggccgcaatt gctccacaga gtgtcgttgc aatcgccaca gtgagtgtgc
     6961 tggcgttgga gcacgggatc actgcctgct gtgccggaac cacaccaagg gtagccactg
     7021 tgagcagtgt ctcccactgt ttgtgggttc ggctttgggt ggcgggacct gccggccatg
     7081 ccatgccttc tgtcgaggga acagccatgt gtgtgtctcc aggaaagagc tagaaatggc
     7141 cagaagggag ccagagaagt actcactgga tccagaggag attgaagcct gggtggcaga
     7201 agggcctagt gaagatgaag ctgtgtgtgt gaactgccag aacaacagct acggagacag
     7261 atgtgagagc tgcctgcacg gctacttcct cctggacggg aaatgcacca agtgccagtg
     7321 caatggccac gctgacacct gtaacgagca ggacggaaca ggatgcccat gtcagaacaa
     7381 cacagagaca ggcgtctgcc agggcagctc tcctagtgac cgccgagact gctacaagta
     7441 ccagtgtgct aagtgccggg agtcattcca cgggagccca ctgggtggcc agcagtgtta
     7501 ccgactcatc tcggtggagc aggagtgctg cctggacccc acttcccaga ccaactgctt
     7561 ccatgagccg aagcgccggg ccttgggtcc tggccgaact gtgctgtttg gcgtccaacc
     7621 caagttcacc aatgtggaca tccgcctgac actagacgtg accttcggtg ctgtggacct
     7681 ctatgtctcc acttcctatg acacttttgt ggtccgagtg gcccctgaca caggtgtcca
     7741 cactgtgcac atccagccac ccccacctcc cccacctcct ccgcctcctg ccgatggtgt
     7801 acctcgtgtg gcttcggacc taggaggact ggggaccgga agtggatcag gttctccagt
     7861 ggagccacga gtccgcgagg tgtggccacg gggcctgatt acctatgtga cagtgacgga
     7921 accctcggca gtgctggtgg tccgcagtgt gcgggacagg ctggtgatca cctatcccca
     7981 tgagcaccac gccctcaagt ccagccgctt ctacctgctg ctgctggggg taggggatcc
     8041 caacgggcct ggggccaacg gctcggcgga ctcacagggc ctgctcttct tccggcaaga
     8101 ccaggcccac attgacctgt tcgtcttctt ctctgtcttc ttttcctgct tcttcctctt
     8161 cctctcactc tgtgtgctcc tctggaaggc caagcaggct ctggaccagc ggcaagagca
     8221 gcgccggcac ctgcaggaga tgaccaagat ggctagccgc ccctttgcca aggtcacagt
     8281 ctgcttccct ccagaccctg cgggcccagc acctgcctgg aagccagctg gcctcccgcc
     8341 tcctgccttc cgccgctccg agccatttct tgcacctcta ctgctgacag gagctggggg
     8401 gccctgggga cccatgggag gagggtgctg cccaccagct ctccctgcca ccacagctgg
     8461 cctacgagct gggcccatta cccttgaacc cacagaagat ggcatggctg gtgtggccac
     8521 tctgctgctc cagctacctg gggggcctca tgctcccaat ggggcctgcc ttgggtcagc
     8581 ccttgtcact ctgcgtcata ggctgcatga gtactgtggg ggaagcgggg gtgctggggg
     8641 cagtggacat gggggtggtg gaggccggaa gggactgtta agtcaggaca atctcactag
     8701 catgtccctt tgacctgtca agggttctct tccgcagtta ccacgttgcc tgatggggcc
     8761 actctgggct agagatcccc tggacagtcc tcagaaacca ctggctccct ttctccaggg
     8821 tctccccttt gttctgcatc tagcaactgt ttattgagct actactgtgt gcctcgtaca
     8881 gttgtaggca cagagcaagg caggaggtga tagcccccac ccccatctgc taggacttga
     8941 gttcttgtgg gaggagacac gcacatgtaa cacagtgagg catccaggta aagtggatgc
     9001 ggcacttgga gaaactgggc tgagaaaggt gacagaatga gggaagacct ttggaacgca
     9061 aatttcaaga gttgaggaaa ctgtaaggcc gaagggcctg aggctggaag cggtttgttt
     9121 tattacaaga accaggaacc cggccatcaa gtgtagactg agctgggttt tattctcagg
     9181 gcagggaaag tggttgggcc gttctgtgga ggtaaatgga gagtctcagc tctggggggg
     9241 aagagtgggc tgatgggcac agagaggctg ctgcaggtgg gcaactgggc agaaggaggg
     9301 tgagtggcag gatgtgacag tggtgcagac attagggtaa aggagaggac atatccagaa
     9361 tggccactgg gtggacagtg aggtcattac taagctggga caggctgggg cttgtgggct
     9421 ggagccgggt gtgactggat tcatacggca agagcttgag tgcatcaaca ggagggatct
     9481 agtgcactgc ggtgagctgg caaaggcagg caggtacggg gaggacagga cagtgcaact
     9541 acccgctgta ggcacagtga gctgctgcct accctgggct ggaccagggt ctctagctga
     9601 acctggagat gtaggcttat ttcaagtgag cctcagaggc aaatccttca cctgagatgc
     9661 ctcacaccct gctctggcag gtgtggggtg ggggatcctg gaggggtcat catttggctc
     9721 tttttgagtc cttgggactc tcctcagagg ccaccccaac ctgggtctcc cttttggact
     9781 taggagctct ctcttccccc agtcctcggc aggtgagctt gggactcctg ctctccccag
     9841 gacacagctt caagcctcgg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
     9901 tgctgctgct gctgctgctg ctttggtcct gggatggggg ttgggcctag cctctttccc
     9961 agtctgatag gtcctgataa tgtgcatgtg tcagcagggc tcacatttct tttttttttt
    10021 ttttttttgg ttcttttttt tggagctggg gaccgaaccc agggccttgc gcttcctagg
    10081 taagcgctct accactgagc taaatcccca gccccagggc tcacatttct acaacaggag
    10141 ggcaactgag gcagcaataa agtgtgggga agactcggga gggcggcttg actgccttct
    10201 gtgtgccggg gatgagggat gctctgggtg agtggaacat gtgtcctggt cgcacctcca
    10261 agctgtgtat cctgagccta gctctgcccc ttctcagcaa tgcagggtgc tgcaacgggg
    10321 tctcctgctc cccggaagag ggatggcagg aacaatgggc agggacatct ttgaccgttt
    10381 ctttgtccct gccacagttc ggctcaggga gaatcagaca gtctgtgtca cagggattgt
    10441 ccttagataa gaaaagccca ggtacgtacg gaacacagaa accattgttt gaacgggagg
    10501 aattgagcac agagaactgg tagtgctggt gagggcagca gctgcaggac agtggcagct
    10561 gggtgtggac aagcattctc agaagtaagc tcgacatcaa agctgagaaa ctattacccc
    10621 cccaccccgc cttcccatcc tagccctcgg ccccggacac caaaatctgg acgcaagggc
    10681 agaggtggac ctgacatctc ggcctcagct ccacacggtt ctttgctgca gaggctgaac
    10741 agtgcatttt gtctgtatgc ttggccccaa tgccagtccc ttgcttcagt gcatggccgt
    10801 gtggcgcatg agtgacagta cgttcaggcg gtactctggc cccatgtgag ttttgctttc
    10861 atgagctgac agtgtctcct gccctgtgaa atgtgaacat aaacttggct tttaaaaaaa
    10921 gtaattttgt gtaagtatgt gtgcatgtgg gtggaggtgc cctaaggagg ccaggagaag
    10981 gcatcagatc ctctcaaact agagttgcag gcagtcatga gctgccatgt gggtgccggg
    11041 aaccaaagtg ctccttccca agggcagcca gagctcttaa ccactgagcc atctctcact
    11101 gtagtcggtt tacatattaa gagctgctac catcagaggt gaacacaagc ttggaaaggt
    11161 ttatccgttc cccaagattg cacggaaatg gtaaggtaaa gactgggtcc caggagttct
    11221 gagacccagc atgttccagt taattccttc tgcagtacgt gtcgccgggc gaaacgtcac
    11281 tgagttgaag aggtggtaat gaacttagta tgatgagcta gctcatgggg gaccaccgtt
    11341 ggtggccttc ccccaaaggc ccatcttgtc tggaagcaga tgggaggaca ggtgtggggg
    11401 atccaactcc ctttgggaac tccatgtatg tggtgtgtgc aggcacaaga cacacacaca
    11461 caattaaaat tcctttttaa agtatctgta gatactgagc gagctccgga gagcattgtt
    11521 tgaagagttt aagtctttct ttcttaatat tttacaagtt ttctcttatt tgaagcaggg
    11581 tctcaatatg cggcccaggc tgccctcatt ccatgcagcc tgggcttctc ttagacttcc
    11641 tgcctcagca tctgtagcgt ggggattgca gcaggcttgg gcgtctatcc ccagactgtc
    11701 tggttttgct tattttcagg atgtgggcag gacttcagat aggtcatctg cagcctcagg
    11761 ctaatggttc tactttgtga ggtgctccca ggaagtcctc acaggcccac ccctagcaaa
    11821 cccgtcttgt ctgatacacc tagatgtctc tctcagcaaa cctcccaatg cacccagagt
    11881 ccttccccaa gacaataaaa acttgtaggt ggggttggtt ttcg
//
