LOCUS       NM_001108673             648 bp    mRNA    linear   ROD 21-MAR-2023
DEFINITION  Rattus norvegicus mediator complex subunit 8 (Med8), mRNA.
ACCESSION   NM_001108673 XM_001072348 XM_342894
VERSION     NM_001108673.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Tsai KL, Tomomori-Sato C, Sato S, Conaway RC, Conaway JW and
            Asturias FJ.
  TITLE     Subunit architecture and functional modular rearrangements of the
            transcriptional mediator complex
  JOURNAL   Cell 157 (6), 1430-1444 (2014)
   PUBMED   24882805
  REMARK    Erratum:[Cell. 2014 Jul 17;158(2):463]
REFERENCE   2  (bases 1 to 648)
  AUTHORS   Krebs AR, Demmers J, Karmodiya K, Chang NC, Chang AC and Tora L.
  TITLE     ATAC and Mediator coactivators form a stable complex and regulate a
            set of non-coding RNA genes
  JOURNAL   EMBO Rep 11 (7), 541-547 (2010)
   PUBMED   20508642
REFERENCE   3  (bases 1 to 648)
  AUTHORS   Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens
            L, Zybailov B, Washburn MP, Brower CS, Conaway RC and Conaway JW.
  TITLE     A mammalian mediator subunit that shares properties with
            Saccharomyces cerevisiae mediator subunit Cse2
  JOURNAL   J Biol Chem 279 (7), 5846-5851 (2004)
   PUBMED   14638676
REFERENCE   4  (bases 1 to 648)
  AUTHORS   Brower CS, Sato S, Tomomori-Sato C, Kamura T, Pause A, Stearman R,
            Klausner RD, Malik S, Lane WS, Sorokina I, Roeder RG, Conaway JW
            and Conaway RC.
  TITLE     Mammalian mediator subunit mMED8 is an Elongin BC-interacting
            protein that can assemble with Cul2 and Rbx1 to reconstitute a
            ubiquitin ligase
  JOURNAL   Proc Natl Acad Sci U S A 99 (16), 10353-10358 (2002)
   PUBMED   12149480
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH474008.2.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_342894.3, XM_001072348.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CB758415.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN06680412, SAMN06680419
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..648
                     /gene="Med8"
                     /note="mediator complex subunit 8"
                     /db_xref="GeneID:362575"
                     /db_xref="RGD:1305552"
     exon            1..265
                     /gene="Med8"
                     /inference="alignment:Splign:2.1.0"
     CDS             222..410
                     /gene="Med8"
                     /note="mediator of RNA polymerase II transcription,
                     subunit 8 homolog"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 8"
                     /protein_id="NP_001102143.1"
                     /db_xref="GeneID:362575"
                     /db_xref="RGD:1305552"
                     /translation="MAHVTYLSFLEVHSRENAKWNKNQHQVSFNASLPAVSVDGSLHS
                     QVICAELSNEIIFCSRLI"
     exon            266..648
                     /gene="Med8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atcacggaat aacaagctgg cagttctgca gtccttcctg gatccttccc tgcaccgagc
       61 ccctgtcccc tggcctcggc ttcctgcctt ctctcgtgtg catttctgtt tactgtgaga
      121 gcactctggt ggggttcatg gaggcagctt cgaagctctg tgggcagctg tctagggaga
      181 aggacaactg agaacacagt tctgcttccc tgtgacattg aatggcccac gtgacttacc
      241 tgtcttttct agaagtccat tctagggaaa atgccaagtg gaataaaaac caacatcaag
      301 tcagcttcaa tgcatcccta ccagcggtga gtgtggacgg cagcctccac tctcaggtga
      361 tctgtgctga gttgagcaat gaaattatct tttgctcaag gcttatttag atgggtacaa
      421 taaaagatgc aggctttcag caagagacag atctcagctc cactgtgaat agccatggaa
      481 cttgggaaaa tgacctattt tttcctgagc cgtgttcttg tctgtaaata gtgataatac
      541 ctacctcata gggttgttgt gaggattgaa atgggagagt gaatgtaaga cacttagcac
      601 ggtatacaaa atactgggtc ttgaataaat aatagtttcc tacttttc
//
