LOCUS       NM_001399687             889 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus mediator complex subunit 30 (Med30), transcript
            variant 1, mRNA.
ACCESSION   NM_001399687 XM_006241646
VERSION     NM_001399687.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Poss,Z.C., Ebmeier,C.C. and Taatjes,D.J.
  TITLE     The Mediator complex and transcription regulation
  JOURNAL   Crit Rev Biochem Mol Biol 48 (6), 575-608 (2013)
   PUBMED   24088064
  REMARK    Review article
REFERENCE   2  (bases 1 to 889)
  AUTHORS   Kagey,M.H., Newman,J.J., Bilodeau,S., Zhan,Y., Orlando,D.A., van
            Berkum,N.L., Ebmeier,C.C., Goossens,J., Rahl,P.B., Levine,S.S.,
            Taatjes,D.J., Dekker,J. and Young,R.A.
  TITLE     Mediator and cohesin connect gene expression and chromatin
            architecture
  JOURNAL   Nature 467 (7314), 430-435 (2010)
   PUBMED   20720539
  REMARK    Erratum:[Nature. 2011 Apr 14;472(7342):247]
REFERENCE   3  (bases 1 to 889)
  AUTHORS   Malik,S., Guermah,M., Yuan,C.X., Wu,W., Yamamura,S. and Roeder,R.G.
  TITLE     Structural and functional organization of TRAP220, the
            TRAP/mediator subunit that is targeted by nuclear receptors
  JOURNAL   Mol Cell Biol 24 (18), 8244-8254 (2004)
   PUBMED   15340084
  REMARK    Erratum:[Mol Cell Biol. 2022 Nov 17;42(11):e0035922. PMID:
            36255214]
REFERENCE   4  (bases 1 to 889)
  AUTHORS   Wang,Q., Sharma,D., Ren,Y. and Fondell,J.D.
  TITLE     A coregulatory role for the TRAP-mediator complex in androgen
            receptor-mediated gene expression
  JOURNAL   J Biol Chem 277 (45), 42852-42858 (2002)
   PUBMED   12218053
REFERENCE   5  (bases 1 to 889)
  AUTHORS   Brower,C.S., Sato,S., Tomomori-Sato,C., Kamura,T., Pause,A.,
            Stearman,R., Klausner,R.D., Malik,S., Lane,W.S., Sorokina,I.,
            Roeder,R.G., Conaway,J.W. and Conaway,R.C.
  TITLE     Mammalian mediator subunit mMED8 is an Elongin BC-interacting
            protein that can assemble with Cul2 and Rbx1 to reconstitute a
            ubiquitin ligase
  JOURNAL   Proc Natl Acad Sci U S A 99 (16), 10353-10358 (2002)
   PUBMED   12149480
REFERENCE   6  (bases 1 to 889)
  AUTHORS   Kang,Y.K., Guermah,M., Yuan,C.X. and Roeder,R.G.
  TITLE     The TRAP/Mediator coactivator complex interacts directly with
            estrogen receptors alpha and beta through the TRAP220 subunit and
            directly enhances estrogen receptor function in vitro
  JOURNAL   Proc Natl Acad Sci U S A 99 (5), 2642-2647 (2002)
   PUBMED   11867769
REFERENCE   7  (bases 1 to 889)
  AUTHORS   Naar,A.M., Beaurang,P.A., Zhou,S., Abraham,S., Solomon,W. and
            Tjian,R.
  TITLE     Composite co-activator ARC mediates chromatin-directed
            transcriptional activation
  JOURNAL   Nature 398 (6730), 828-832 (1999)
   PUBMED   10235267
REFERENCE   8  (bases 1 to 889)
  AUTHORS   Ito,M., Yuan,C.X., Malik,S., Gu,W., Fondell,J.D., Yamamura,S.,
            Fu,Z.Y., Zhang,X., Qin,J. and Roeder,R.G.
  TITLE     Identity between TRAP and SMCC complexes indicates novel pathways
            for the function of nuclear receptors and diverse mammalian
            activators
  JOURNAL   Mol Cell 3 (3), 361-370 (1999)
   PUBMED   10198638
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Jan 12, 2022 this sequence version replaced XM_006241646.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CA504616.1, CF109457.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-259               JAXUCZ010000007.1  85894638-85894896
            260-418             JAXUCZ010000007.1  85901809-85901967
            419-523             JAXUCZ010000007.1  85905130-85905234
            524-889             JAXUCZ010000007.1  85916008-85916373
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q31"
     gene            1..889
                     /gene="Med30"
                     /gene_synonym="Thrap6"
                     /note="mediator complex subunit 30"
                     /db_xref="GeneID:299905"
                     /db_xref="RGD:1309369"
     exon            1..259
                     /gene="Med30"
                     /gene_synonym="Thrap6"
                     /inference="alignment:Splign:2.1.0"
     CDS             83..619
                     /gene="Med30"
                     /gene_synonym="Thrap6"
                     /note="isoform 1 is encoded by transcript variant 1;
                     thyroid hormone receptor associated protein 6; mediator of
                     RNA polymerase II transcription subunit 30"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 30 isoform 1"
                     /protein_id="NP_001386616.1"
                     /db_xref="GeneID:299905"
                     /db_xref="RGD:1309369"
                     /translation="MSTPPLAPTGMASGPFGGPQAQQAAREVNTATLCRIGQETVQDI
                     VYRTMEIFQLLRNMQLPNGVTYHTGTYQDRLTKLQDHLRQLSILFRKLRLVYDKCNEN
                     CGGMDPIPVEQLIPYVDEDGSKNDDRAGPPRFASEERREIAEVNKKLKQKNQQLKQIM
                     DQLRNLIWDINAMLAMRN"
     exon            260..418
                     /gene="Med30"
                     /gene_synonym="Thrap6"
                     /inference="alignment:Splign:2.1.0"
     exon            419..523
                     /gene="Med30"
                     /gene_synonym="Thrap6"
                     /inference="alignment:Splign:2.1.0"
     exon            524..889
                     /gene="Med30"
                     /gene_synonym="Thrap6"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgtcgcggc aggcggaagt ggcgccgctt gtttgaaatt ggcagagtag gctccggccg
       61 gacccgagcc gctgggggtg ccatgtccac ccctccgctg gcgcccacgg gcatggcgtc
      121 cggacccttc ggcggcccgc aggcgcagca ggccgcgcgc gaggtcaaca cggccacgct
      181 gtgccgcatc gggcaggaga ccgtgcaaga catcgtgtac cgcaccatgg agatcttcca
      241 gctgctcagg aacatgcagc tgccaaatgg cgtcacttac catactggaa cttaccaaga
      301 ccggttaaca aagctacagg accatcttcg gcaactttct attctcttca ggaagctacg
      361 actggtctat gacaagtgta atgagaactg tggtggaatg gaccccatcc ctgtggagca
      421 actgattcca tatgtggatg aagacggctc aaagaatgat gaccgggctg gtccacctcg
      481 ttttgctagt gaagagagac gagaaattgc agaagtaaat aagaaactca aacagaagaa
      541 tcaacagctg aagcagatta tggatcaatt acggaatctc atctgggaca taaatgccat
      601 gttggcaatg aggaactaaa ctgatattta aatctcctgc tcatgtgata cgattgtctc
      661 ccccctccct tgagtattcc tccctttgaa aaacagtatt tatgtcttta ttttaacagc
      721 tagcactaaa gtttttcttt tcactttaaa gtatttacta gctttttttt ttttaatgct
      781 gtgggtttta tgaaagatta ttataatacc tttgataggg tgtgagcttt ggttaatctt
      841 cataaattga atgaattaaa aaatacaaat aaaaatttat acataacta
//
