LOCUS       NM_001277054            4727 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Rattus norvegicus mediator complex subunit 23 (Med23), mRNA.
ACCESSION   NM_001277054 XM_001053256 XM_002725463 XM_002728640 XM_003748671
            XM_003753148 XM_220093
VERSION     NM_001277054.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Kasper,L.H., Fukuyama,T. and Brindle,P.K.
  TITLE     T-cells null for the MED23 subunit of mediator express decreased
            levels of KLF2 and inefficiently populate the peripheral lymphoid
            organs
  JOURNAL   PLoS One 9 (7), e102076 (2014)
   PUBMED   25054639
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Poss,Z.C., Ebmeier,C.C. and Taatjes,D.J.
  TITLE     The Mediator complex and transcription regulation
  JOURNAL   Crit Rev Biochem Mol Biol 48 (6), 575-608 (2013)
   PUBMED   24088064
  REMARK    Review article
REFERENCE   3  (bases 1 to 4727)
  AUTHORS   Krebs,A.R., Demmers,J., Karmodiya,K., Chang,N.C., Chang,A.C. and
            Tora,L.
  TITLE     ATAC and Mediator coactivators form a stable complex and regulate a
            set of non-coding RNA genes
  JOURNAL   EMBO Rep 11 (7), 541-547 (2010)
   PUBMED   20508642
REFERENCE   4  (bases 1 to 4727)
  AUTHORS   Malik,S., Guermah,M., Yuan,C.X., Wu,W., Yamamura,S. and Roeder,R.G.
  TITLE     Structural and functional organization of TRAP220, the
            TRAP/mediator subunit that is targeted by nuclear receptors
  JOURNAL   Mol Cell Biol 24 (18), 8244-8254 (2004)
   PUBMED   15340084
  REMARK    Erratum:[Mol Cell Biol. 2022 Nov 17;42(11):e0035922. doi:
            10.1128/mcb.00359-22.. PMID: 36255214]
REFERENCE   5  (bases 1 to 4727)
  AUTHORS   Brower,C.S., Sato,S., Tomomori-Sato,C., Kamura,T., Pause,A.,
            Stearman,R., Klausner,R.D., Malik,S., Lane,W.S., Sorokina,I.,
            Roeder,R.G., Conaway,J.W. and Conaway,R.C.
  TITLE     Mammalian mediator subunit mMED8 is an Elongin BC-interacting
            protein that can assemble with Cul2 and Rbx1 to reconstitute a
            ubiquitin ligase
  JOURNAL   Proc Natl Acad Sci U S A 99 (16), 10353-10358 (2002)
   PUBMED   12149480
REFERENCE   6  (bases 1 to 4727)
  AUTHORS   Ryu,S., Zhou,S., Ladurner,A.G. and Tjian,R.
  TITLE     The transcriptional cofactor complex CRSP is required for activity
            of the enhancer-binding protein Sp1
  JOURNAL   Nature 397 (6718), 446-450 (1999)
   PUBMED   9989412
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On or before Mar 2, 2013 this sequence version replaced
            XM_220093.6, XM_002728640.2, XM_003748671.1, XM_001053256.3,
            XM_002725463.2, XM_003753148.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643293.306.1, SRR26643299.276.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-128               JAXUCZ010000001.1  22356627-22356754   c
            129-160             JAXUCZ010000001.1  22356303-22356334   c
            161-248             JAXUCZ010000001.1  22356057-22356144   c
            249-373             JAXUCZ010000001.1  22354200-22354324   c
            374-485             JAXUCZ010000001.1  22353009-22353120   c
            486-584             JAXUCZ010000001.1  22351924-22352022   c
            585-686             JAXUCZ010000001.1  22350131-22350232   c
            687-756             JAXUCZ010000001.1  22342138-22342207   c
            757-869             JAXUCZ010000001.1  22340743-22340855   c
            870-965             JAXUCZ010000001.1  22338204-22338299   c
            966-1166            JAXUCZ010000001.1  22332390-22332590   c
            1167-1310           JAXUCZ010000001.1  22330894-22331037   c
            1311-1456           JAXUCZ010000001.1  22330286-22330431   c
            1457-1702           JAXUCZ010000001.1  22329095-22329340   c
            1703-1845           JAXUCZ010000001.1  22327510-22327652   c
            1846-2020           JAXUCZ010000001.1  22325972-22326146   c
            2021-2184           JAXUCZ010000001.1  22324783-22324946   c
            2185-2309           JAXUCZ010000001.1  22322607-22322731   c
            2310-2515           JAXUCZ010000001.1  22321300-22321505   c
            2516-2696           JAXUCZ010000001.1  22321029-22321209   c
            2697-2867           JAXUCZ010000001.1  22319683-22319853   c
            2868-3084           JAXUCZ010000001.1  22319118-22319334   c
            3085-3321           JAXUCZ010000001.1  22317468-22317704   c
            3322-3487           JAXUCZ010000001.1  22315949-22316114   c
            3488-3560           JAXUCZ010000001.1  22315258-22315330   c
            3561-3775           JAXUCZ010000001.1  22314055-22314269   c
            3776-3896           JAXUCZ010000001.1  22312888-22313008   c
            3897-4028           JAXUCZ010000001.1  22311921-22312052   c
            4029-4727           JAXUCZ010000001.1  22309613-22310311   c
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1p12"
     gene            1..4727
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /note="mediator complex subunit 23"
                     /db_xref="GeneID:309565"
                     /db_xref="RGD:1307671"
     exon            1..128
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..4193
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /note="CRSP complex subunit 3; cofactor required for Sp1
                     transcriptional activation subunit 3"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 23"
                     /protein_id="NP_001263983.1"
                     /db_xref="GeneID:309565"
                     /db_xref="RGD:1307671"
                     /translation="MVPMETQLQSIFEEVVKTEIIEEAFPGMFMDTPEDEKTKLISCL
                     AAFRQFWNGLSQESHEQCVQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRMVCE
                     SLINSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVV
                     QQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPT
                     ARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYV
                     LEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLW
                     QHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNAL
                     ADFLPVMKLFDLLYPEKECIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPI
                     PHSLKLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIM
                     RVPLPGTSCLASASVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHTKSSVALAPAL
                     VETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSHRMHHIQPHYRV
                     QLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAE
                     SEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMTFTPHNWASHTLSCFPA
                     PLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMTDENEIITQFSMQGFPPLFLCLLWK
                     MLLETDHISQIGYKVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDM
                     VWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEH
                     WLQSDWHTKHMSYHKKYPEKLYFEGLAEQVDPPVPIQSPYLPIYFGNVCLRFLPVFDI
                     VIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMCLRNRDHLKRKLV
                     HAIIGSLKDNRPQGWCLSDTYLKHAMNAREDNPWVPEDSYYCKLIGRLVDTMAGKSPG
                     PFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENIT
                     AWMNAIGLIITALPEPYWIVLHDRIVSVISSSSLTSETEWVGYPFRLFDFTACHQSYS
                     EMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPVVKTEFQLLYVYHLVGPFLQRF
                     QQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKI
                     ICNLKPALKLRLRFITHISKMEPAVPPQALSSGSPAPQANQVPTALPVTQ"
     exon            129..160
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            161..248
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            249..373
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            374..485
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            486..584
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            585..686
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            687..756
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            757..869
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            870..965
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1166
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1310
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1311..1456
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1457..1702
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1845
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..2020
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..2184
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            2185..2309
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            2310..2515
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            2516..2696
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            2697..2867
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            2868..3084
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            3085..3321
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            3322..3487
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            3488..3560
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            3561..3775
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            3776..3896
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            3897..4028
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
     exon            4029..4727
                     /gene="Med23"
                     /gene_synonym="Crsp3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctccgcccc ctcgctccat tcttcccagg tctaggggtt cacccggtgg ttgccgggta
       61 ccccggaagg ccgggactcg atggtaccaa tggagacgca actgcagagc attttcgagg
      121 aggtggtgaa aacagaaatt atagaagagg ctttcccagg catgtttatg gatacccccg
      181 aggatgaaaa aacaaaactt attagctgtt tggcagcctt ccggcagttt tggaatggac
      241 tttctcagga gtctcatgaa cagtgtgtcc agtggattgt taaatttatt cacggccaac
      301 atagcccgaa aaggatttcc tttctctatg actgcttagc catggcagtt gagactggtc
      361 ttcttccacc caggatggtt tgtgaatctt tgataaactc tgacactctt gaatgggaaa
      421 gaacacagct ttgggcctta acatttaagc tggttcggaa aatcattgga ggagtggatt
      481 ataagggtgt tcgagacctc ctgaaagtga ttttggagaa aatcttgaca atcccgaata
      541 ctgtaagctc tgctgttgtc cagcagcttc tggcagcaag agaggttata gcgtatatct
      601 tggaacgaaa tgcctgcctg ttgccagcct actttgcagt cacagagatc agaaaattat
      661 atcccgaggg aaaacttcca cactggttac taggaaactt ggtatcagac tttgtggata
      721 ccttcaggcc cacagcgagg ataaactcca tttgtggccg ttgcagtctt ctgccagttg
      781 tgaacaactc gggagccatc tgtaactcat ggaaactgga ccctgcaact ctccgctttc
      841 ccttgaaagg ccttttgcca tacgataagg atctgtttga accccagact gctttgttga
      901 gatatgtact ggagcagcct tactccaggg atatggtctg caatatgcta ggtttaaaca
      961 agcagcacaa gcagcgctgc cctgtgctgg aggaccagtt ggtggacctg gtggtgtacg
     1021 ccatggagag gtctgagacc gaggagaagt ttgacgatgg gggaaccagc cagctcctgt
     1081 ggcagcacct ttccagccag ctcattttct ttgtgctttt ccagtttgcc agtttcccac
     1141 atatggttct gtctctccac cagaagttag cagggcgagg actgattaaa gggcgagacc
     1201 atctgatgtg ggttctgctg cagttcatct cgggaagtat tcagaaaaat gcgcttgctg
     1261 acttcctccc tgtcatgaag ctctttgacc tgctgtaccc agagaaggaa tgtatcccag
     1321 ttcctgatat taacaaacca cagtcaacac atgcctttgc aatgacttgt atttggattc
     1381 atcttaatag aaaagctcaa aatgacaact ccaagctgca gatccccata ccccactccc
     1441 tgaagctgca ccacgagttc ctacagcaga gtctgagaaa caaaagtttg cagatgaatg
     1501 actataagat cgccctgctg tgtaatgcgt attctacaaa ttcagagtgt ttcaccttac
     1561 ctatgggcgc tctggtagag accatttatg gcaatggaat tatgagagtg cctctccccg
     1621 ggacaagctg tctggcctca gcatcagtaa ctcccttacc aatgaacctc ctggattctc
     1681 tgacagtgca tgccaagatg agccttattc acagcattgc aaccagagtg ataaaactgg
     1741 ctcatacaaa atccagtgtc gcattggctc cagccctggt ggaaacttac agtcgtttac
     1801 tggtctacat ggaaatagaa tctttgggca tcaaaggatt tatcagtcag ctcctgccca
     1861 ctgtcttcaa gtcccatgcc tggggcatcc tgcacacgct gctggagatg ttcagccacc
     1921 ggatgcacca cattcagcca cattaccgag ttcagctcct gagccatctc cacaccctgg
     1981 ctgcagttgc acaaaccaac cagaaccagc tccatctgtg cgtagaaagt actgcgctga
     2041 ggctcatcac ggcgctgggg agctccgagg tccagccgca gtttacacgc ttcctcaacg
     2101 atcccaaaac agtgctgtcc gcagaatctg aagagctgaa ccgagccttg atcctgacct
     2161 tggccagagc aacccacgtc acagattttt ttacaggatc tgattcaata cagggaactt
     2221 ggtgtaaaga tatccttcag accatcatga cttttactcc tcataactgg gcttcgcaca
     2281 cccttagctg ttttccagcc cctctgcagg cattcttcaa gcaaaataat gtacctcagg
     2341 aaagccgttt taatctgaaa aaaaacgtgg aagaagagta caggaagtgg aagtcaatga
     2401 ctgatgaaaa cgagatcatc acccagttct ccatgcaagg cttccctccg ctcttcctct
     2461 gtctcctctg gaaaatgctc ctggaaacgg atcacattag tcagattggg tacaaagtac
     2521 tagagagaat tggggccagg gccttggtgg cccacgtgag aacctttgca gatttcttgg
     2581 tgtatgagtt ctctacatca gcaggaggtc agcaactcaa taaatgcatt gagattctca
     2641 atgacatggt gtggaagtac aacattgtca cactggacag actaattctc tgcctggcta
     2701 tgcgtagtca tgaaggaaat gaagctcagg tttgttattt cataattcag ttgctattgc
     2761 taaaaccaaa tgacttccga aaccgagtga gtgactttgt gaaggagaac tccccagagc
     2821 actggctgca gagcgactgg cacaccaaac acatgagcta ccacaagaaa tatccagaga
     2881 agttgtactt tgaaggcctg gcagaacagg ttgaccctcc tgtaccaatt cagtccccgt
     2941 atctgcccat ctactttgga aacgtgtgtc tccggtttct tccagtattt gatatagtga
     3001 tccacaggtt tttagagttg ctgccagtgt ccaaatcact agagactctc ctggatcacc
     3061 tagggggctt gtacaaattc cacgaccggc cggtgactta cctgtataac actttacact
     3121 attatgaaat gtgcctgaga aaccgtgacc atctcaaacg aaaactcgtc catgccatca
     3181 ttggctccct caaagataac cggccacagg gctggtgtct cagtgacacc tacctgaagc
     3241 atgctatgaa tgcacgggag gacaacccct gggttccaga ggactcctac tactgcaagc
     3301 tgattggccg gctggtagac accatggctg gcaagtctcc gggacccttc ccaaactgtg
     3361 actggagatt caatgagttc cccaaccctg ctgcccatgc tctgcatgtg acgtgtgtgg
     3421 aactcatggc cttggcagtt ccaggcaaag atgtcgggaa tgcccttctc aatgttgtcc
     3481 tgaaaagcca gcccttagtg ccaagggaga acatcacagc atggatgaat gcaattggtc
     3541 tgatcatcac ggccctccca gaaccatact ggattgtgct tcatgaccga atcgtgagtg
     3601 taatcagcag ttctagcctg acgtcagaga cagagtgggt tggttatccg ttccgcctct
     3661 tcgacttcac cgcttgtcac cagtcctact ctgagatgag ctgcagctac acgttagcgc
     3721 tggctcacgc cgtgtggcac cactccagca tcggacagct ctcgcttatc ccgaagtttc
     3781 tcactgaagt gcttcttccc gtggtgaaga ctgaattcca gttgctttac gtgtaccatc
     3841 tcgtagggcc atttttacag cgattccagc aggagagaac ccggtgcatg atcgagattg
     3901 gagtggcatt ttatgacatg ctgctgaatg tagaccagtg cagcacccac ctcaattaca
     3961 tggaccccat ctgcgacttc ctataccata tgaagtatat gttcactgga gacagtgtga
     4021 aggagcaggt agagaagatc atctgtaatt taaagccagc tttgaagctt cgtcttcggt
     4081 tcatcacgca catcagcaag atggagccgg cagtgccacc ccaggccctc agcagcgggt
     4141 ctccagcccc tcaggccaac caggtgccca cagctctgcc cgtgactcag tgagacctgg
     4201 ggcactgtga cgaagcagag gaggggactc acggctaaat ctgcgaaacc cgtcagcggc
     4261 catgtccaga ttggtttatt ctgattcaga tactgaatct ctttacacct tcaaatccct
     4321 tctccctgtc tatgttttat ctctaagaga ttaagtaagc tctgtgttgt tcatattcct
     4381 atcatttatt ggggccctgg gaatgtgggt gtttctgtag tcacgtggct ttgaccaggc
     4441 actcccaggc ttcctgtctc agccacttgt aaattattgt acgtgacact tcacattttc
     4501 tatacatttc cttgcagaca caacacgtgt gcaatggtca ttgcaaaccc ttttatacat
     4561 aaaatgtcct tccaaataca ttaattacag ggccttatac ttcacttgga gtacaggaca
     4621 aatgaagttt catagttttt tttatttaac tccctataaa ttttttattg taaattttaa
     4681 atttccaaat agttccaaat aaatgatgta aagtttctac caaagta
//
