LOCUS       NM_001107895             791 bp    mRNA    linear   ROD 23-MAR-2023
DEFINITION  Rattus norvegicus mediator complex subunit 21 (Med21), mRNA.
ACCESSION   NM_001107895 XM_001074060 XM_232531
VERSION     NM_001107895.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Poss ZC, Ebmeier CC and Taatjes DJ.
  TITLE     The Mediator complex and transcription regulation
  JOURNAL   Crit Rev Biochem Mol Biol 48 (6), 575-608 (2013)
   PUBMED   24088064
  REMARK    Review article
REFERENCE   2  (bases 1 to 791)
  AUTHORS   Kagey MH, Newman JJ, Bilodeau S, Zhan Y, Orlando DA, van Berkum NL,
            Ebmeier CC, Goossens J, Rahl PB, Levine SS, Taatjes DJ, Dekker J
            and Young RA.
  TITLE     Mediator and cohesin connect gene expression and chromatin
            architecture
  JOURNAL   Nature 467 (7314), 430-435 (2010)
   PUBMED   20720539
  REMARK    Erratum:[Nature. 2011 Apr 14;472(7342):247]
REFERENCE   3  (bases 1 to 791)
  AUTHORS   Krebs AR, Demmers J, Karmodiya K, Chang NC, Chang AC and Tora L.
  TITLE     ATAC and Mediator coactivators form a stable complex and regulate a
            set of non-coding RNA genes
  JOURNAL   EMBO Rep 11 (7), 541-547 (2010)
   PUBMED   20508642
REFERENCE   4  (bases 1 to 791)
  AUTHORS   Malik S, Guermah M, Yuan CX, Wu W, Yamamura S and Roeder RG.
  TITLE     Structural and functional organization of TRAP220, the
            TRAP/mediator subunit that is targeted by nuclear receptors
  JOURNAL   Mol Cell Biol 24 (18), 8244-8254 (2004)
   PUBMED   15340084
  REMARK    Erratum:[Mol Cell Biol. 2022 Nov 17;42(11):e0035922. PMID:
            36255214]
REFERENCE   5  (bases 1 to 791)
  AUTHORS   Brower CS, Sato S, Tomomori-Sato C, Kamura T, Pause A, Stearman R,
            Klausner RD, Malik S, Lane WS, Sorokina I, Roeder RG, Conaway JW
            and Conaway RC.
  TITLE     Mammalian mediator subunit mMED8 is an Elongin BC-interacting
            protein that can assemble with Cul2 and Rbx1 to reconstitute a
            ubiquitin ligase
  JOURNAL   Proc Natl Acad Sci U S A 99 (16), 10353-10358 (2002)
   PUBMED   12149480
REFERENCE   6  (bases 1 to 791)
  AUTHORS   Ito M, Okano HJ, Darnell RB and Roeder RG.
  TITLE     The TRAP100 component of the TRAP/Mediator complex is essential in
            broad transcriptional events and development
  JOURNAL   EMBO J 21 (13), 3464-3475 (2002)
   PUBMED   12093747
REFERENCE   7  (bases 1 to 791)
  AUTHORS   Ge K, Guermah M, Yuan CX, Ito M, Wallberg AE, Spiegelman BM and
            Roeder RG.
  TITLE     Transcription coactivator TRAP220 is required for PPAR gamma
            2-stimulated adipogenesis
  JOURNAL   Nature 417 (6888), 563-567 (2002)
   PUBMED   12037571
REFERENCE   8  (bases 1 to 791)
  AUTHORS   Tudor M, Murray PJ, Onufryk C, Jaenisch R and Young RA.
  TITLE     Ubiquitous expression and embryonic requirement for RNA polymerase
            II coactivator subunit Srb7 in mice
  JOURNAL   Genes Dev 13 (18), 2365-2368 (1999)
   PUBMED   10500093
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            FM087109.1 and CN544042.1.
            
            On May 26, 2012 this sequence version replaced NM_001107895.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FM087109.1, FM118367.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132263
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-524               FM087109.1         1-524
            525-791             CN544042.1         1-267               c
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q44"
     gene            1..791
                     /gene="Med21"
                     /gene_synonym="Surb7"
                     /note="mediator complex subunit 21"
                     /db_xref="GeneID:312849"
                     /db_xref="RGD:1309836"
     exon            1..71
                     /gene="Med21"
                     /gene_synonym="Surb7"
                     /inference="alignment:Splign:2.1.0"
     CDS             30..464
                     /gene="Med21"
                     /gene_synonym="Surb7"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 21"
                     /protein_id="NP_001101365.2"
                     /db_xref="GeneID:312849"
                     /db_xref="RGD:1309836"
                     /translation="MADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFSNIQTAINK
                     DQPANPTEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAAT
                     CLEDVVYRGDMLLEKIQSALADIAQSQLKTRSVTHSQSLPDS"
     exon            72..186
                     /gene="Med21"
                     /gene_synonym="Surb7"
                     /inference="alignment:Splign:2.1.0"
     exon            187..287
                     /gene="Med21"
                     /gene_synonym="Surb7"
                     /inference="alignment:Splign:2.1.0"
     exon            288..774
                     /gene="Med21"
                     /gene_synonym="Surb7"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttttgcgcac cgctgcgtgc gcggagaaca tggcggatag actcacgcaa ctgcaggacg
       61 ctgtgaactc gcttgcagat cagttttgca atgccattgg tgtattacag cagtgtggtc
      121 cccctgcctc ttttagtaac attcagacag caattaataa agatcaacca gccaatccta
      181 cagaagagta tgcccagctt tttgcagcac tgattgcacg aacagcaaaa gatattgatg
      241 ttttgattga ttccttaccc agtgaagagt ctacagctgc tttacaggct gctagcttat
      301 ataagctaga agaagaaaat cacgaagctg ctacgtgtct agaggatgtt gtttatcggg
      361 gagacatgct tctggagaag atccaaagtg cacttgctga cattgcacag tcacagctga
      421 agaccagaag tgttacccat agtcagtctc tcccagactc atagcatcaa tggctgagat
      481 gtgacggaga agagaactgt ttgaatggca ttaagaatgc cgcatcagat ggaagcccta
      541 cggacagctg tagaacatca tatgctatga cggtgttgcc tttttaagct agttttcatt
      601 gtttgctgtt ttcactcttg ataagtaaaa gactttacaa ttataacgga atcatcttca
      661 aacatcatta tgccatcgtt ttttatctga ctagaatttt caggacagat actgtactaa
      721 ggttcatgat ataattaaac agatatacaa taaagctctt tgctgttttg tgtaaaaaaa
      781 aaaaaaaaaa a
//
