LOCUS       NM_001013178            1964 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Rattus norvegicus mediator complex subunit 20 (Med20), transcript
            variant 1, mRNA.
ACCESSION   NM_001013178 XM_236917
VERSION     NM_001013178.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Poss,Z.C., Ebmeier,C.C. and Taatjes,D.J.
  TITLE     The Mediator complex and transcription regulation
  JOURNAL   Crit Rev Biochem Mol Biol 48 (6), 575-608 (2013)
   PUBMED   24088064
  REMARK    Review article
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Rajan,S., Chu Pham Dang,H., Djambazian,H., Zuzan,H., Fedyshyn,Y.,
            Ketela,T., Moffat,J., Hudson,T.J. and Sladek,R.
  TITLE     Analysis of early C2C12 myogenesis identifies stably and
            differentially expressed transcriptional regulators whose
            knock-down inhibits myoblast differentiation
  JOURNAL   Physiol Genomics 44 (2), 183-197 (2012)
   PUBMED   22147266
REFERENCE   3  (bases 1 to 1964)
  AUTHORS   Krebs,A.R., Demmers,J., Karmodiya,K., Chang,N.C., Chang,A.C. and
            Tora,L.
  TITLE     ATAC and Mediator coactivators form a stable complex and regulate a
            set of non-coding RNA genes
  JOURNAL   EMBO Rep 11 (7), 541-547 (2010)
   PUBMED   20508642
REFERENCE   4  (bases 1 to 1964)
  AUTHORS   Malik,S., Guermah,M., Yuan,C.X., Wu,W., Yamamura,S. and Roeder,R.G.
  TITLE     Structural and functional organization of TRAP220, the
            TRAP/mediator subunit that is targeted by nuclear receptors
  JOURNAL   Mol Cell Biol 24 (18), 8244-8254 (2004)
   PUBMED   15340084
  REMARK    Erratum:[Mol Cell Biol. 2022 Nov 17;42(11):e0035922. doi:
            10.1128/mcb.00359-22.. PMID: 36255214]
REFERENCE   5  (bases 1 to 1964)
  AUTHORS   Brower,C.S., Sato,S., Tomomori-Sato,C., Kamura,T., Pause,A.,
            Stearman,R., Klausner,R.D., Malik,S., Lane,W.S., Sorokina,I.,
            Roeder,R.G., Conaway,J.W. and Conaway,R.C.
  TITLE     Mammalian mediator subunit mMED8 is an Elongin BC-interacting
            protein that can assemble with Cul2 and Rbx1 to reconstitute a
            ubiquitin ligase
  JOURNAL   Proc Natl Acad Sci U S A 99 (16), 10353-10358 (2002)
   PUBMED   12149480
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000009.1 and FN804985.1.
            
            On Apr 5, 2013 this sequence version replaced NM_001013178.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC083734.1, FQ183942.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN12840105,
                                           SAMN12840108 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                JAXUCZ010000009.1  20879974-20880011   c
            39-799              FN804985.1         1-761
            800-1964            JAXUCZ010000009.1  20867681-20868845   c
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q12"
     gene            1..1964
                     /gene="Med20"
                     /gene_synonym="Trfp; Usp49"
                     /note="mediator complex subunit 20"
                     /db_xref="GeneID:316209"
                     /db_xref="RGD:1306415"
     exon            1..127
                     /gene="Med20"
                     /gene_synonym="Trfp; Usp49"
                     /inference="alignment:Splign:2.1.0"
     CDS             114..752
                     /gene="Med20"
                     /gene_synonym="Trfp; Usp49"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mediator of RNA polymerase II transcription subunit 20;
                     Trf (TATA binding protein-related factor)-proximal protein
                     homolog; ubiquitin specific protease 49; Trf-proximal
                     protein homolog-like"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 20 isoform 1"
                     /protein_id="NP_001013196.1"
                     /db_xref="GeneID:316209"
                     /db_xref="RGD:1306415"
                     /translation="MGVTCVSQMPVAEGKSVQQTVELLTKKLEMLGAEKQGTFCVDCE
                     TYHTAASTLGSQGQAGKLMYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFF
                     QSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISVEVEYGPCVVASDCWSLLLEF
                     LQSFLGSHTPGAPTVFGNRHDAVYGPADTMVQYMELFNKVRKQQQVPVAGIR"
     exon            128..282
                     /gene="Med20"
                     /gene_synonym="Trfp; Usp49"
                     /inference="alignment:Splign:2.1.0"
     exon            283..536
                     /gene="Med20"
                     /gene_synonym="Trfp; Usp49"
                     /inference="alignment:Splign:2.1.0"
     exon            537..1964
                     /gene="Med20"
                     /gene_synonym="Trfp; Usp49"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tttccggagg ccggtcaacg accgcttcct gtgtttaggg ggctgcctct cgtgggaagg
       61 tctggattct ctgagtttga gtatttggca gtaccacctt ttgacccttc gccatgggag
      121 tgacgtgtgt gtcccagatg cctgtggctg agggcaagag tgtccagcag acagtggagc
      181 tcctcaccaa gaaattggag atgcttggag cagagaaaca aggaacattt tgtgtggact
      241 gtgaaactta ccacacagct gcctccaccc tgggcagcca aggccaggct gggaagctga
      301 tgtacgtgat gcataactct gagtacccac tgagctgctt tgccctcttt gagaatggcc
      361 cttgccttat tgctgacacc aacttcgatg tgctcatggt gaagctcaag ggctttttcc
      421 agagtgccaa ggccagcaag attgagaccc ggggtactcg ctaccagtat tgtgacttcc
      481 tggtgaaggt gggcacggtc acaatggggc ccagtgcccg tggcatctct gtggaggtgg
      541 agtatggccc ttgtgtggta gctagtgact gctggagcct cctgcttgaa ttcctacaga
      601 gttttctagg tagccacaca ccaggggcgc ccacagtgtt tgggaacaga catgatgcgg
      661 tctacgggcc agcagatacc atggtacagt acatggagct cttcaacaag gtccggaaac
      721 agcagcaagt gcctgtggct gggatccgct agtgactggc agctgccgcc tgctgagctg
      781 tcaccatggc actgtcaagg accacgtgct ggacttgggt cccagctgta gcttgtgttc
      841 cagggctttg gactcccctc ctgaccacat cacagctgtt ctttacttca tgctttaact
      901 ggatttggct ggtcctcaag aatggtaatt gtgaagaatg gaaaggcttg ggcctttcct
      961 cctgtaggca aaaggtagga cagggttgtg cccaccagtg ctagagctgt gtgtccccag
     1021 gtgctgagca tttcgaatag gctgtatgcc tgtgctgctt tcgcttgagg atgtggctgg
     1081 caggacggga ttgttggctg cgagagtgtt gctttcattc tttggacact gatgtcttcc
     1141 cctactgttt tgaattatgt gaacctgcag gccaagttgc tgtagcagaa ccaaaagcag
     1201 agggctctta ctgcacagct tatttttcgt taatagttca gaggcattca gttccacaga
     1261 acagaatgaa ctccacaggg ttaccaggaa cttggcttct tatgtcttac tgttccttct
     1321 atcatactgt ccccagaatg ctttcctcac cacaggatca aagcacgctc agtatctccg
     1381 tgcccacagt ctgctgagga aagagatagg gatgtttttc aaagttgatt agatcactgt
     1441 ggacacacat tcctgacact caacactcag ttttctcagg gggcttccta gtttgccgtc
     1501 ttggctggct tgagccttga actcactgac attgctcacc tgcctcacac ctgttacaga
     1561 aacatttaac acgtagacag gcttgggaaa gctgttcata tacaaagtgt agggtggcag
     1621 tgtttgtgca gcagctctga ggaggtgggt cattcttgtg tcctagcttt ggtgggcagg
     1681 acagctcatc acaccttcct ggttatcctg ttacttcctc ttgcttttgt tgttactctt
     1741 ttattgttgc tgtgtaaaaa ctgaggtgtt ttagatacca caataaaatt gacaaacact
     1801 acagtttcgg ccttctgttc tgtgtttggt cttgttgcag gctgtctact ttgtactttg
     1861 tactggtcgc ttgaaaacac agatgttaag tgtggtaaat atggactctc agttcctaca
     1921 taagaaggga tcagtttcta aaaaataaag agaagcacaa acaa
//
