LOCUS       NM_001415972            5583 bp    mRNA    linear   ROD 22-SEP-2024
DEFINITION  Rattus norvegicus malic enzyme 3 (Me3), mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_001415972 XM_039082375
VERSION     NM_001415972.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Hasan,N.M., Longacre,M.J., Stoker,S.W., Kendrick,M.A. and
            MacDonald,M.J.
  TITLE     Mitochondrial malic enzyme 3 is important for insulin secretion in
            pancreatic beta-cells
  JOURNAL   Mol Endocrinol 29 (3), 396-410 (2015)
   PUBMED   25594249
  REMARK    GeneRIF: The data suggest that ME3, far more than ME1 or ME2, is
            necessary for insulin release.
REFERENCE   3  (bases 1 to 5583)
  AUTHORS   Loeber,G., Maurer-Fogy,I. and Schwendenwein,R.
  TITLE     Purification, cDNA cloning and heterologous expression of the human
            mitochondrial NADP(+)-dependent malic enzyme
  JOURNAL   Biochem J 304 (Pt 3) (Pt 3), 687-692 (1994)
   PUBMED   7818469
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Jan 6, 2023 this sequence version replaced XM_039082375.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643289.2192.1,
                                           SRR26643296.2018.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-141               JAXUCZ010000001.1  152946628-152946768
            142-339             JAXUCZ010000001.1  152947086-152947283
            340-473             JAXUCZ010000001.1  153036777-153036910
            474-623             JAXUCZ010000001.1  153040595-153040744
            624-699             JAXUCZ010000001.1  153077414-153077489
            700-861             JAXUCZ010000001.1  153087496-153087657
            862-965             JAXUCZ010000001.1  153096604-153096707
            966-1075            JAXUCZ010000001.1  153120546-153120655
            1076-1173           JAXUCZ010000001.1  153138374-153138471
            1174-1287           JAXUCZ010000001.1  153138762-153138875
            1288-1393           JAXUCZ010000001.1  153140589-153140694
            1394-1536           JAXUCZ010000001.1  153141288-153141430
            1537-1710           JAXUCZ010000001.1  153141968-153142141
            1711-1809           JAXUCZ010000001.1  153142847-153142945
            1810-5583           JAXUCZ010000001.1  153144253-153148026
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q32"
     gene            1..5583
                     /gene="Me3"
                     /note="malic enzyme 3"
                     /db_xref="GeneID:361602"
                     /db_xref="RGD:1311070"
     exon            1..141
                     /gene="Me3"
                     /inference="alignment:Splign:2.1.0"
     exon            142..339
                     /gene="Me3"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..1971
                     /gene="Me3"
                     /EC_number="1.1.1.40"
                     /note="mitochondrial malic enzyme 3; malic enzyme 3,
                     NADP(+)-dependent, mitochondrial"
                     /codon_start=1
                     /product="NADP-dependent malic enzyme, mitochondrial"
                     /protein_id="NP_001402901.1"
                     /db_xref="GeneID:361602"
                     /db_xref="RGD:1311070"
                     /translation="MGAALGTGARLTSLPRLACGSLRRQALSAPAQGCHSKSGPPRPV
                     PLKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYENQQS
                     DLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFI
                     TIHDKGHIATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGV
                     NPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVRGKEYDDLLDEFMQAVTDKFGINCL
                     IQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNRLSNHVFVF
                     QGAGEAAMGIAHLLVMALEKEGIPKTEAVRKIWMVDSKGLIVKGRSHLNHEKEMFAQE
                     HPEVNSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAEC
                     TAEKCYQVTEGRGIFASGSPFKSVTLEDGRTFIPGQGNNAYVFPGVALGIIAGGIRHV
                     PDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAVKVLDYAYKHNLASYYP
                     EPKDKEAFIKSLIYTPDYDSFSLDTYTWPKEAMSVQKV"
     exon            340..473
                     /gene="Me3"
                     /inference="alignment:Splign:2.1.0"
     exon            474..623
                     /gene="Me3"
                     /inference="alignment:Splign:2.1.0"
     exon            624..699
                     /gene="Me3"
                     /inference="alignment:Splign:2.1.0"
     exon            700..861
                     /gene="Me3"
                     /inference="alignment:Splign:2.1.0"
     exon            862..965
                     /gene="Me3"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1075
                     /gene="Me3"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1173
                     /gene="Me3"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1287
                     /gene="Me3"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1393
                     /gene="Me3"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..1536
                     /gene="Me3"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1710
                     /gene="Me3"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..1809
                     /gene="Me3"
                     /inference="alignment:Splign:2.1.0"
     exon            1810..5583
                     /gene="Me3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtccctcgt actgcctgag tggtgcggtg ctgcctttgg ccagagtgtg acaggggaca
       61 gtagcgtctt tccctacctg cctgcagtgg ggctgcacag acttgagctt ctcacagact
      121 tgagcttctc cagttaggca ggtctgtgcc aggaccatgg gcgccgcgct gggcactggc
      181 gcacggctaa cctccctacc tcgcctagcc tgcggcagcc ttcggcgtca ggcactctcc
      241 gcgcccgccc agggatgcca ctccaagtcc ggtccgcctc gcccagtacc cctgaagaag
      301 cgcggatacg atgtaaccag gaacccccat ctcaacaagg gcatggcctt tacactggaa
      361 gagaggctgc agcttggcat ccacggcctg atccctccct gcttcctcag ccaggatgtc
      421 cagctcctcc gcatcatgag atactatgag aatcagcaga gcgacctgga caagtacatc
      481 atcctcatga ctctccaaga ccgcaatgag aagctcttct accgagtgct gacctcagac
      541 gtggagaagt tcatgcccat tgtgtacaca cccactgtgg ggctggcctg tcagcactat
      601 ggcctcacct tccgccgacc tcgtgggctg tttatcacca tccatgacaa gggccacatt
      661 gcaacaatgc tgaactcttg gccagaggac aatattaagg ctgtggtggt gactgatgga
      721 gagcgcatac tgggcttggg agacctgggc tgctacggca tgggcatccc tgtgggcaag
      781 ctggccctgt acacagcatg cggaggggtg aacccacagc agtgccttcc tgtcctgcta
      841 gatgtcggca ccaacaatga ggagcttctc agagaccctc tgtacatcgg cctgaaacac
      901 caacgtgttc gtgggaagga gtatgatgac ctgctggatg aattcatgca ggctgtgaca
      961 gataagttcg gaataaattg cctcatccag tttgaagact ttgccaatgc caatgccttc
     1021 cgtctactca acaaataccg caacaaatac tgcatgttca atgatgacat ccaaggcaca
     1081 gcctctgtcg ctgtggcagg gatcctggct gcactgagaa tcaccaagaa caggctgtcc
     1141 aatcatgtgt ttgttttcca gggtgcgggt gaggcagcca tgggcattgc ccacctcctt
     1201 gtcatggccc tggaaaagga aggcataccc aagacagagg ccgtaaggaa gatctggatg
     1261 gtggactcca agggtctgat tgtcaagggt agaagccacc taaaccatga gaaggagatg
     1321 tttgcccaag agcatcctga agtcaactcc ctggaggagg tagtaaggct ggtgaagccc
     1381 acagcaatta taggtgtcgc tgccatcgca ggagccttca cggagcagat tctgagggac
     1441 atggcctcct tccatgagcg ccctatcatc tttgccctga gcaaccccac cagcaaggct
     1501 gagtgcacag ctgagaagtg ctaccaggtc accgagggcc gaggaatctt tgccagtgga
     1561 agtcctttta agagtgtgac tctggaagat ggcaggacat tcattcctgg tcagggaaac
     1621 aatgcctatg tgtttcccgg ggtagcactg ggcatcattg ctggtgggat cagacatgtg
     1681 ccagatgaaa ttttcctcct gacagcagag caaattgccc aagaagtctc tgagcagcac
     1741 ctgtcccagg ggagactgta cccaccactc agcaccatcc gagatgtgtc tctgagaatt
     1801 gcagtcaaag tcctcgacta tgcgtacaaa cacaacctgg cctcctacta tccagagccc
     1861 aaggacaagg aggcttttat aaaatctctg atctacactc cagactatga ctccttctcg
     1921 ctagacacct acacctggcc taaggaagct atgagtgttc agaaggtctg acgccatctc
     1981 cagagattgt ttgggctcaa tgaagaaata caatataaac aggttccaaa atggccagtc
     2041 ttgtcaccat ggttacggtt gtcatttgtt ttcctttggg tgttggggta agatgctaag
     2101 gcatacagcg gtaacagcct tgttcctgaa ggacctgcag gctcaccttc ctataaccct
     2161 tttctgaatt cctttctgga tatttctttt actattaact gctaactatt aaccaaacct
     2221 tgtggggaaa ttctgcctag aatcctgcct ttgtagagct caaagtctag atcagacaca
     2281 agtcccattt cataagaaag tgtgatgtcc acgaaaaaaa aaaaagttca tgtagaagtc
     2341 agagtgctcg gagaagaatg ggcacttcct ataaagacac aaaaggagac ctggctttgt
     2401 actttgccca agagtgaagg atgggttaag tcataagcac tcaagaaatg catgcaaaac
     2461 tacatacgag aaaatgaggt ccacccatca gggaactaat ccctttcaga agaatacatt
     2521 aatttcaaga ttgagtagga catgggggct gagtggctag aggctcactg ccctttgggt
     2581 tgttgggtac tgtgcattct ttttggtcat tactaaaagg aaaactagag aattgggggt
     2641 aggaagggtc agcatgccaa aaactgagga aaacgaggag caatgagagc aaacaccgtg
     2701 gggaaggtgt ttaagtctaa gaaagacttg atgtaatgtt tcaggttcca gaacactctc
     2761 ctctccatcc ttcacacaga cacaattgag cagtttgctt ctagaggtga catcagtctg
     2821 cctttggtgt ttttaaggcc tgtttagtga aaacgtctac ctcctccatg ttcaggagtg
     2881 cacacactcc aagacaaaca gtaaagaatg gcttggagcc cctgctgtga gctgtgtcct
     2941 tttactgcat aagtcattct taagacctac cctgctgtct ccttggccag gattcctgct
     3001 gcacataatg agtaggttct atatgactgt ctctcaggtc aagccaggta aattactaaa
     3061 atccatggag aatattttgc acattcatga atcaactgcc ccttgtttgg gtcttattct
     3121 tccaatacca gcttcttggg tgctagtcct caaccaagga tatccccaaa gcatagacct
     3181 aaatgcagaa agaaaaacaa tgcatttcta gaagagaata aagcagtgag tccttgtgaa
     3241 gactccccag taaacctctg attacaatct cacatgcaat gcctgtgtcc ctggggatac
     3301 aattcacaaa agatggaaaa aactggctaa ggtttccaat tttctgatct attgcatcag
     3361 ccccacatgg ctgccaagaa tcttgttgga tttctcatct cctcaggaga aacaatctgt
     3421 ctggacccaa cctaatcctt tttattccat gtccagactc agtaaatgca cccagagaag
     3481 aaaatggctg cttatgccag aaaggatccc tccatggaat ccgatactgc tagttaatat
     3541 cttttgtccc agtgttatta ggtgtgtgag gtttagcact gttataattt atggtgaact
     3601 caaaactgta aaccttggtg atttactaaa taatgtattt gttttgtcgt gccatatctt
     3661 agatatcata tcttggaagt ctctcttgca attcattatt tattcagatg cttacttaag
     3721 cttacagagt acctaatgtt tgtaaacatt ctccagttat ttctcccaca gaactcaaac
     3781 gattttttcc caaagtgaaa atatggctgt cttaacatta aaacatagaa tcatttcatc
     3841 agcttcacgg taaaaatgat tatcaagaaa cctaatttaa atcacatcac ttgaaaaaca
     3901 taaaacatta catgtccaaa ttcttagtag aaactcttgt tcaggttgag aacacatggg
     3961 aaaatatcag actttaaagc taagtaatag taatttctaa tgtttgacca aaaaaaaaat
     4021 aggagtttca gaaactggat aattaaacac cgttttagat acaaagacaa cataactcta
     4081 cttttattta aaaaaaaatc caggggcagg ggagaaggct cagtaagtca tgagcgtgcc
     4141 atgcaagaac tgagttcaac ccctagagca tatgtaaaaa cgctgagagc agcaggatgc
     4201 acttgtaacc ccagcactga ggtagacgtc aaggagacag ggggatcctg gggctcaatg
     4261 accaaccagc cttgctcaat tggcaagctc caggtaaaag ttgagagacc ttatttcaaa
     4321 acagcaaaga agagagcaac tgaggaagcc acttgccatt tgacgttaaa cctctgctgt
     4381 gtgcatgtgt acacccacat acattcaaat acacatatga gatgcctact aaatgaaaca
     4441 ctatcccagc acagtatttt atttcacagt cttcccctag tctatcctca gccctcctcc
     4501 tctaattcat acgccaaaca tgaacattta ttatggcaag gcattcttca tcagaagaca
     4561 ttaagcacac gctgcttctg tgcagaatgg atctcgcatg agcttctatc aaagctgcct
     4621 tttctaacta gaagtttcaa aattgcatag ggtgagattt atggaaatgc acttgatcac
     4681 tgtgaaactt aaagtcccac agaagcaacc gttgcacaaa cttgacgtgc gaccagtgca
     4741 cattcaagaa ggcgagcacc gaagagcagg taggtagcta tgtgtctgca gtgtttgaag
     4801 tgacttaagg aataagccac agaaggtttg gggcgctgtt tatattttgg ggggaatttt
     4861 gtgtaactaa aaccagacaa gtactgtgtc acgtcttggc agccaatctc actaaacttc
     4921 ttgcgtatct cacatgtgac agtaacaatc ctaacaggtt gcacttttat caagcactta
     4981 gctttaatat tcctgtggaa acattattag attcactcac taagcctata ttgttgacta
     5041 aaatgtgcag taaatttggt ctaatctcag gctccacacc caaggaaaca gcataaagca
     5101 gccatcttgt attaaatgct tttttccatt ggagaccctg aaaagcagcc gcacttcaga
     5161 acactgtttc tctaagaagt tgtttgctat agaaagctct gccgcacgtc gctgtcctgt
     5221 aaaccgtagc tcctcctcat ttttcactgc acttaccgtt aatgctaaag caacaagcac
     5281 tggggctctc ccaggacctg cagcacaacg cagagcaata cagcaaccgg gctcctcccg
     5341 aagtttcatg ttttcaagac ttaaccagtc gtcatggacg gtgttgggcc atcatcaaaa
     5401 agccagccta gaacctggat accttgtttc tcaaccagag ccctatctta agttgtgtca
     5461 cacacccatg tgtgtactgc cctggtaatt ctgtagttct taaatccctc taaatctgtt
     5521 ctgggttgca ccggttgggt tatgagtggt aagaaatctc atattcctat taagagaact
     5581 cca
//
