LOCUS       NM_001107376            2899 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus malic enzyme 2 (Me2), mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_001107376 XM_001053085 XM_225729
VERSION     NM_001107376.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Liu,F., Jiang,L.J., Zhang,Y.X., Xu,S.T., Liu,S.L., Ye,J.T. and
            Liu,P.Q.
  TITLE     Inhibition of miR-214-3p attenuates ferroptosis in myocardial
            infarction via regulating ME2
  JOURNAL   Biochem Biophys Res Commun 661, 64-74 (2023)
   PUBMED   37087800
  REMARK    GeneRIF: Inhibition of miR-214-3p attenuates ferroptosis in
            myocardial infarction via regulating ME2.
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Piao,L., Fang,Y.H., Parikh,K., Ryan,J.J., Toth,P.T. and Archer,S.L.
  TITLE     Cardiac glutaminolysis: a maladaptive cancer metabolism pathway in
            the right ventricle in pulmonary hypertension
  JOURNAL   J Mol Med (Berl) 91 (10), 1185-1197 (2013)
   PUBMED   23794090
REFERENCE   3  (bases 1 to 2899)
  AUTHORS   Jiang,P., Du,W., Mancuso,A., Wellen,K.E. and Yang,X.
  TITLE     Reciprocal regulation of p53 and malic enzymes modulates metabolism
            and senescence
  JOURNAL   Nature 493 (7434), 689-693 (2013)
   PUBMED   23334421
REFERENCE   4  (bases 1 to 2899)
  AUTHORS   MacDonald,M.J., Longacre,M.J. and Kendrick,M.A.
  TITLE     Mitochondrial malic enzyme (ME2) in pancreatic islets of the human,
            rat and mouse and clonal insulinoma cells
  JOURNAL   Arch Biochem Biophys 488 (2), 100-104 (2009)
   PUBMED   19691144
  REMARK    GeneRIF: Me2 activity in Islets of Langerhans cells was measured by
            a spectrophotometric enzyme assay by utilizing the distinct kinetic
            properties of Me2.
REFERENCE   5  (bases 1 to 2899)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000018.1, CB556640.1, EV771591.1, CB794492.1, BF522605.1
            and CV796911.1.
            
            On Jan 24, 2009 this sequence version replaced NM_001107376.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643429.54663.1,
                                           SRR26643292.60225.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760389,
                                           SAMEA5760393 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-54                JAXUCZ010000018.1  69676165-69676218   c
            55-739              CB556640.1         1-685
            740-928             EV771591.1         544-732
            929-964             CB794492.1         393-428
            965-1016            JAXUCZ010000018.1  69654044-69654095   c
            1017-1338           BF522605.1         10-331
            1339-1849           CV796911.1         79-589              c
            1850-2899           JAXUCZ010000018.1  69631034-69632083   c
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="18"
                     /map="18q12.2"
     gene            1..2899
                     /gene="Me2"
                     /note="malic enzyme 2"
                     /db_xref="GeneID:307270"
                     /db_xref="RGD:1310321"
     exon            1..89
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
     exon            90..207
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
     CDS             100..1854
                     /gene="Me2"
                     /EC_number="1.1.1.38"
                     /note="malic enzyme 2, NAD(+)-dependent, mitochondrial"
                     /codon_start=1
                     /product="NAD-dependent malic enzyme, mitochondrial"
                     /protein_id="NP_001100846.2"
                     /db_xref="GeneID:307270"
                     /db_xref="RGD:1310321"
                     /translation="MFSRLRAVTTPCTLTCRRVHLKEKGKPLMLNPRTNKGMAFTLQE
                     RQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQD
                     DIESLMPIVYTPTVGLACCQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVV
                     TDGERILGLGDLGVYGMGIPVGKLCLYTACAGIQPEKCLPVCIDVGTDNMALLKDPFY
                     MGLYQKRDRSQLYDDLMDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLKKYQEKYCTF
                     NDDIQGTAAVALSGLLAAQRVINKPISEHRILFLGAGEAALGIAKLIVISMMESGLSE
                     EEAQRKIWMFDKNGLLVKGRTARIDSNQEPYAHAASENIPATFEDAVNKLKPSVIIGV
                     AGAGPLFTPGVIKAMAAINERPIIFALSNPTAQAECTAEDAYTLTEGRCLFASGSPFE
                     PVRLQDGRVFTPGQGNNAYIFPGVALAVILCEARHISDTVFLEAAKALTSQLTDEELA
                     QGRLYPSLANIQEVSANIAIKIAEYLYANKMAFRYPEPEDKAKYVKERIWRSNYVSLL
                     PDVYDWPESSLKPPQIPE"
     exon            208..341
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
     exon            342..491
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
     exon            492..567
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
     exon            568..729
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
     exon            730..833
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
     exon            834..943
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1041
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1155
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1270
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1413
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
     exon            1414..1516
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
     exon            1517..1587
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1686
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..2899
                     /gene="Me2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggcagggcg gcgccagggc cgagcatctc tggctatagc gccgcggcct ctctgcttgc
       61 tgcactacct ggcacgggcc aggcgtccgg agagagaaga tgttttcccg gttaagagct
      121 gttaccactc cttgcacttt gacctgccgc agagtgcacc tgaaggagaa gggcaagcca
      181 ctcatgttga atccaagaac aaacaagggg atggcgttta cattacaaga acgacaaatg
      241 cttggtcttc aaggactttt acctcctaaa atagagacac aggatattca agccttacga
      301 ttccatagaa acttgaaaaa aatgactagc cctttggaaa aatacatcta tataatgggc
      361 atacaagaaa gaaacgagaa actgttttat agaatactac aggatgatat tgaaagtctg
      421 atgccaattg tgtacacacc aacagttggt cttgcctgtt gtcaatacgg acatatcttt
      481 aggagaccta agggattgtt tatttcaatc tcagacagag gtcatgttag atcaattgtg
      541 gataactggc cagaaaatca tgttaaggct gttgtggtga ctgatggaga gagaatcctg
      601 ggacttggag atttgggtgt ctatggaatg gggattccag tcgggaagct ttgcttgtat
      661 acagcatgcg caggaatcca gcctgagaaa tgcctgcctg tgtgcatcga tgtagggact
      721 gataacatgg ctctactgaa agacccgttt tatatgggct tgtatcagaa gcgagaccgc
      781 tcccagctct atgacgatct gatggatgag tttatgaagg cgatcactga cagatatggc
      841 cggaacacac ttattcagtt tgaagacttt ggaaatcata atgcattcag attcttaaaa
      901 aaataccagg aaaaatactg taccttcaat gacgacattc aagggactgc tgcagttgcc
      961 ttgtctggtc tccttgcggc ccaaagagtg attaataaac caatctcaga acacagaatc
     1021 ttattcctcg gggcaggaga ggctgcactt ggaattgcga agcttatagt catatcaatg
     1081 atggaaagtg gtctctcaga agaggaggca cagagaaaga tctggatgtt cgacaagaac
     1141 ggcttgctag ttaagggccg gactgctaga atagatagta accaggaacc atatgctcat
     1201 gctgcctcag agaacatacc tgcaaccttt gaagatgcag taaataaact taagccttca
     1261 gttataattg gagtggcagg tgctggcccg ctttttactc ctggcgtaat taaagccatg
     1321 gccgctatca acgagaggcc cataatattt gctttaagca atcctacagc acaggccgag
     1381 tgcactgccg aagacgctta cacacttaca gagggcaggt gcttgtttgc cagtggcagc
     1441 ccatttgagc cggtgagact ccaggatggg cgtgtcttca ccccgggcca agggaacaat
     1501 gcatacatct tcccaggtgt ggctttagct gttattctct gtgaagcccg acacatcagt
     1561 gacaccgttt tcttagaagc tgcaaaggca ctgacatccc aattgacaga tgaagagtta
     1621 gcccaaggga ggctttaccc atcacttgct aatattcagg aagtttctgc taacatagct
     1681 attaaaattg cagagtacct gtacgctaat aaaatggctt tccgataccc agagcccgag
     1741 gacaaggcca aatatgtgaa ggaaaggata tggcggagta attatgtttc cctgctgcca
     1801 gatgtgtatg attggccaga gtcgtcattg aagcctccgc agataccaga atagaagctt
     1861 tccctttaag acattccgtg ctgcagggac aaaaagataa tattctctga gttactgtgt
     1921 cctctggttt attcctccgt actactttgc ttggcatttt tcctgtgaat ctagctttgg
     1981 ttggggacag acatgctgac tatgctgtgg tgcccacagt ccagtactgg gaatgcttca
     2041 ttcagttcag tgggtctcct cgtgtgctct gctttgagct cgccacggta ttgttgctgt
     2101 tacgtgaatg tctctcttct tccactcagt cctctagcac tgtgcatatg agtacaacgt
     2161 gttgagaaga agctaagtgt tcaccaaatc ccaaagaaaa agttgttagc acttaaagct
     2221 gttctttttc ttttctttct tttttttttt tttttttttt tttggagctg gggaccgaac
     2281 ccagggcctt gcgcttgcta ggcaaacgct ctaccactga gctaaatccc ccaccccaag
     2341 ctgttctttt ctatatactt catttcactt tctgctctga aattcctttg tagtagacag
     2401 gtttggggaa ataggaaaac aaaaaaaaag atcccagttt cctagaacta aatcagtgct
     2461 aactgtaaca ttctagaaga atgggaggag ccactgctgc ctttagacag actgaacagc
     2521 catctgcaac agtgtacaaa caagttttac taataaaacc aattccttac gtttaattgc
     2581 ttcagttaca ttatcttatt gaccacacag aaacttaggt ttgttggtta tgacaagagt
     2641 ttcatcatct tcactcagtt aagggagtat catctatcta ccacgcatga ctaatgtcat
     2701 gtgattcgga gatggtgcct aaggggttaa ctacagccta ggtcttcccc ctgtcagtca
     2761 cgtcctttac tgtgcagtaa ttagaggatc tcctcacata cattcctttg aatattagca
     2821 gtgatggctt tctctgctga gaatgcaaaa tagattccaa gattactgga gataaataaa
     2881 gtagaatcag accaggaaa
//
