LOCUS       NM_012600               3075 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus malic enzyme 1 (Me1), mRNA.
ACCESSION   NM_012600
VERSION     NM_012600.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Piao,L., Fang,Y.H., Parikh,K., Ryan,J.J., Toth,P.T. and Archer,S.L.
  TITLE     Cardiac glutaminolysis: a maladaptive cancer metabolism pathway in
            the right ventricle in pulmonary hypertension
  JOURNAL   J Mol Med (Berl) 91 (10), 1185-1197 (2013)
   PUBMED   23794090
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Jiang,P., Du,W., Mancuso,A., Wellen,K.E. and Yang,X.
  TITLE     Reciprocal regulation of p53 and malic enzymes modulates metabolism
            and senescence
  JOURNAL   Nature 493 (7434), 689-693 (2013)
   PUBMED   23334421
REFERENCE   3  (bases 1 to 3075)
  AUTHORS   Heart,E., Cline,G.W., Collis,L.P., Pongratz,R.L., Gray,J.P. and
            Smith,P.J.
  TITLE     Role for malic enzyme, pyruvate carboxylation, and mitochondrial
            malate import in glucose-stimulated insulin secretion
  JOURNAL   Am J Physiol Endocrinol Metab 296 (6), E1354-E1362 (2009)
   PUBMED   19293334
  REMARK    GeneRIF: although ME1 overexpression augments anaplerosis and
            glucose stimulated insulin secretion in INS-1 832/13 cells, it is
            not likely involved in methyl succinate and glucose stimulated
            insulin secretion in pancreatic islets
REFERENCE   4  (bases 1 to 3075)
  AUTHORS   Xu,J., Han,J., Long,Y.S., Lock,J., Weir,G.C., Epstein,P.N. and
            Liu,Y.Q.
  TITLE     Malic enzyme is present in mouse islets and modulates insulin
            secretion
  JOURNAL   Diabetologia 51 (12), 2281-2289 (2008)
   PUBMED   18802677
REFERENCE   5  (bases 1 to 3075)
  AUTHORS   Ronnebaum,S.M., Jensen,M.V., Hohmeier,H.E., Burgess,S.C.,
            Zhou,Y.P., Qian,S., MacNeil,D., Howard,A., Thornberry,N.,
            Ilkayeva,O., Lu,D., Sherry,A.D. and Newgard,C.B.
  TITLE     Silencing of cytosolic or mitochondrial isoforms of malic enzyme
            has no effect on glucose-stimulated insulin secretion from rodent
            islets
  JOURNAL   J Biol Chem 283 (43), 28909-28917 (2008)
   PUBMED   18755687
  REMARK    GeneRIF: cytosolic or mitochondrial isoforms of malic enzyme do not
            affect glucose-stimulated insulin secretion from rodent islets
REFERENCE   6  (bases 1 to 3075)
  AUTHORS   Strait,K.A., Kinlaw,W.B., Mariash,C.N. and Oppenheimer,J.H.
  TITLE     Kinetics of induction by thyroid hormone of the two hepatic mRNAs
            coding for cytosolic malic enzyme in the hypothyroid and euthyroid
            states. Evidence against an obligatory role of S14 protein in malic
            enzyme gene expression
  JOURNAL   J Biol Chem 264 (33), 19784-19789 (1989)
   PUBMED   2584194
REFERENCE   7  (bases 1 to 3075)
  AUTHORS   Morioka,H., Magnuson,M.A., Mitsuhashi,T., Song,M.K., Rall,J.E. and
            Nikodem,V.M.
  TITLE     Structural characterization of the rat malic enzyme gene
  JOURNAL   Proc Natl Acad Sci U S A 86 (13), 4912-4916 (1989)
   PUBMED   2740332
REFERENCE   8  (bases 1 to 3075)
  AUTHORS   Nikodem,V.M., Magnuson,M.A., Dozin,B. and Morioka,H.
  TITLE     Coding nucleotide sequence of rat malic enzyme mRNA and tissue
            specific regulation by thyroid hormone
  JOURNAL   Endocr Res 15 (4), 547-564 (1989)
   PUBMED   2699453
  REMARK    Review article
REFERENCE   9  (bases 1 to 3075)
  AUTHORS   Morioka,H., Tennyson,G.E. and Nikodem,V.M.
  TITLE     Structural and functional analysis of the rat malic enzyme gene
            promoter
  JOURNAL   Mol Cell Biol 8 (8), 3542-3545 (1988)
   PUBMED   3211151
REFERENCE   10 (bases 1 to 3075)
  AUTHORS   Dozin,B., Magnuson,M.A. and Nikodem,V.M.
  TITLE     Tissue-specific regulation of two functional malic enzyme mRNAs by
            triiodothyronine
  JOURNAL   Biochemistry 24 (20), 5581-5586 (1985)
   PUBMED   2416344
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Feb 11, 2021 this sequence version replaced NM_012600.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments and orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ228526.1, SRR26360181.2253743.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760393 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-78                JAXUCZ010000008.1  96540167-96540244   c
            79-212              JAXUCZ010000008.1  96524385-96524518   c
            213-362             JAXUCZ010000008.1  96522551-96522700   c
            363-438             JAXUCZ010000008.1  96498628-96498703   c
            439-600             JAXUCZ010000008.1  96494959-96495120   c
            601-704             JAXUCZ010000008.1  96490154-96490257   c
            705-814             JAXUCZ010000008.1  96464620-96464729   c
            815-912             JAXUCZ010000008.1  96458214-96458311   c
            913-1026            JAXUCZ010000008.1  96456571-96456684   c
            1027-1132           JAXUCZ010000008.1  96443163-96443268   c
            1133-1275           JAXUCZ010000008.1  96442125-96442267   c
            1276-1449           JAXUCZ010000008.1  96440523-96440696   c
            1450-1548           JAXUCZ010000008.1  96433843-96433941   c
            1549-3075           JAXUCZ010000008.1  96429057-96430583   c
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q31"
     gene            1..3075
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /note="malic enzyme 1"
                     /db_xref="GeneID:24552"
                     /db_xref="RGD:3074"
     CDS             1..1719
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /EC_number="1.1.1.40"
                     /note="malate dehydrogenase (oxaloacetate-decarboxylating)
                     (NADP+); malic enzyme 1, soluble; NADP-ME; cytosolic malic
                     enzyme 1; malic enzyme 1, NADP(+)-dependent, cytosolic"
                     /codon_start=1
                     /product="NADP-dependent malic enzyme"
                     /protein_id="NP_036732.3"
                     /db_xref="GeneID:24552"
                     /db_xref="RGD:3074"
                     /translation="MDPRAPRRRHTHQRGYLLTRDPHLNKDLAFTLEERQQLNIHGLL
                     PPCIVNQEIQVLRVIKNFERLNSDFDRYLLLMDLQDRNEKLFYSVLMSNVEKFMPIVY
                     TPTVGLACQQYSLAFRKPRGLFISIHDKGHIASVLNAWPEDVVKAIVVTDGERILGLG
                     DLGCNGMGIPVGKLALYTACGGVNPQQCLPITLDVGTENEELLKDPLYIGLRHRRVRG
                     PEYDAFLDEFMEAASSKYGMNCLIQFEDFANLNAFRLLNKYRNKYCTFNDDIQGTASV
                     AVAGLLAALRITKNKLSDQTVLFQGAGEAALGIAHLIVMAMEKEGLSKEKARQKIWLV
                     DSKGLIVKGRASLTEEKEVFAHEHEEMKNLEAIVQKIKPTALIGVAAIGGAFTEQILK
                     DMAAFNERPIIFALSNPTSKAECSAEECYKVTKGRAIFASGSPFDPVTLPDGRTLFPG
                     QGNNSYVFPGVALGVVACGLRHINDSVFLTTAEVISQQVSDKHLEEGRLYPPLNTIRD
                     VSLKIAVKIVQDAYKEKMATVYPEPQNKEEFVSSQMYSTNYDQILPDCYSWPEEVQKI
                     QTKVNQ"
     exon            1..78
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /inference="alignment:Splign:2.1.0"
     exon            79..212
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /inference="alignment:Splign:2.1.0"
     exon            213..362
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /inference="alignment:Splign:2.1.0"
     exon            363..438
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /inference="alignment:Splign:2.1.0"
     exon            439..600
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /inference="alignment:Splign:2.1.0"
     exon            601..704
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /inference="alignment:Splign:2.1.0"
     exon            705..814
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /inference="alignment:Splign:2.1.0"
     exon            815..912
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1026
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1132
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1275
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1449
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1548
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..3075
                     /gene="Me1"
                     /gene_synonym="MOD1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atggatcccc gagccccccg ccgccgacac acccaccagc gcggctacct gctgacgcgg
       61 gacccgcatc tcaacaagga cttggctttt actctggaag agaggcaaca gctgaacatt
      121 catggcttgt tgccaccctg cattgtcaac caggagatcc aggtccttag agtaattaag
      181 aatttcgagc gtctgaactc tgacttcgac aggtatcttc tgttaatgga tctgcaagat
      241 aggaatgaga agctcttcta cagtgtgctt atgtctaatg ttgaaaagtt catgcctatc
      301 gtttacactc ccaccgtggg tcttgcatgc caacaataca gtttggcatt ccggaagcca
      361 agaggcctct ttatcagtat ccacgacaaa gggcatattg cttcagttct taacgcatgg
      421 ccagaagatg ttgtcaaggc tattgtggtg actgatggag agcgaatcct cggcttgggc
      481 gaccttggtt gtaacgggat gggcatccct gtgggtaaac tggccctgta cacagcgtgc
      541 ggaggggtga atccacaaca gtgtctaccc atcactttgg acgtgggcac agaaaatgag
      601 gagttactta aagatccctt gtatattggg ctgcggcaca ggcgagtgag aggccctgaa
      661 tatgatgcgt ttttggatga attcatggag gcagcgtctt ccaaatatgg catgaattgc
      721 cttattcagt ttgaagattt tgccaatctg aatgcatttc gtctcctgaa caagtatcga
      781 aacaagtatt gcacatttaa cgatgatatt caaggaacag cgtctgtggc agttgccggc
      841 cttcttgctg ctcttcggat aaccaagaac aagctctctg atcagacagt gctgttccag
      901 ggagccggcg aggctgcctt ggggattgct catctgattg ttatggccat ggagaaggaa
      961 ggtttatcaa aggagaaagc tagacaaaag atatggttgg ttgactcaaa aggattaata
     1021 gttaaggggc gtgcttctct cacagaagag aaagaggtgt ttgcccatga acatgaagaa
     1081 atgaagaacc tagaagccat tgttcagaag ataaaaccaa ccgctctcat aggagttgct
     1141 gcaattggtg gtgctttcac agaacaaatt ctcaaggata tggctgcctt caacgagcgg
     1201 cccatcatct ttgctttgag taatccgacc agcaaagctg agtgttctgc agaggagtgc
     1261 tataaagtga ccaagggccg tgcgatcttt gccagcggca gtccttttga tccagtcact
     1321 cttccagatg gacggactct gtttcctggc caaggcaaca actcctatgt gttccctgga
     1381 gttgctcttg gggtagtggc ctgtggactg agacacatca atgattcggt cttcctcacc
     1441 acggctgagg tcatatccca gcaagtgtca gataaacacc tagaagaagg ccggctctat
     1501 cctcctttga acaccatccg agatgtttcc ttgaaaatcg cagtaaagat tgtgcaagat
     1561 gcatacaaag aaaagatggc cactgtttat cctgaacccc aaaacaaaga agaatttgtc
     1621 tcctcccaga tgtacagcac taattatgac cagatcctac ctgattgtta ttcgtggcct
     1681 gaagaagtcc agaaaataca gaccaaagtc aatcagtaac acaacagcta gaatttttaa
     1741 ctttattaat aagatcttga agttttcatg atttttaaga gtcagaatct tttgtgatga
     1801 ttcacagtac gcttagaata aggtgatttt tgtttagccc acagactcat gggagtagat
     1861 tagtgtaagt taggatgaac ttcaccctag acaggtttgt ttcacttact gtggcagttt
     1921 atgttttcac tgtgattatt ctctttacga attctgttta aaagctactg tacctgctgc
     1981 tgagaaagtc ctcactgcta tgtaggaagc taatggaaga cccacactag taataaatta
     2041 atatagcata acttgattac attaaatgcc tacagttctt tcttgactat tttgttaaaa
     2101 tctcctaagc agtaaagata atcacaaact taggcagagt gaacttttac taaaccgaaa
     2161 cactactttg ttgcctaagg aaaaaatctt ctcaggactt tattccaggc ctccgttagc
     2221 tttgttcttt ttgtacgcct gaactctaac tcctctgaga aagctcactg ctgtttacag
     2281 tccttgtgca gccttagatc ataagtctcc ttcactgtta catcccctag agaaaagctc
     2341 tcgttgcctt gacttgggtc ccagacactc cgtagaaaca ccctttctca tctctgacca
     2401 gctcctggac ctgctcagat actggggtgt ttttgttagg gtagcctggg cgctgagaaa
     2461 ggctgcaagg aggaggggcc cctgaggacc cacctctgag acaagggtgt cagatgcaag
     2521 accatggttc ccagacaagc tcgggctcca tgagggtcac ctgccctaat gtccctgggc
     2581 ttagcttaga tgactgagag ctcagggcct gagtaaatac atctcaaacg cctacctttc
     2641 tatcacatat taaaataggt taattactaa aactattctg tgagaaaaag tcagcctttc
     2701 ccaggttgta ttaatttatt ggacaagttg ataacggcat gactagaaac agccttaatt
     2761 cctaagctca ggttggagaa cactctgtct atctagacac actcctggtt ttgaagtcgc
     2821 tttaaagctt gtgtgggttt taccaagggc agctctataa agtgaccccg tgaaggggaa
     2881 gacacggact ctttcaggcg tgacttacag tgtagtcgtt ctaatggtga ctctcactaa
     2941 gggtttcctc agggtagtta tctgggggag agtcaaaaga tctatctttt cttgtctgtt
     3001 tctatgttgt caggtagttg taaatgaatg tatttaccta tgcaaagatt tattaaagcc
     3061 tagagatatg aataa
//
