LOCUS       NM_001398673            1168 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus mitochondrial calcium uniporter dominant negative
            subunit beta (Mcub), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001398673 XM_006233280
VERSION     NM_001398673.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Sancak,Y., Markhard,A.L., Kitami,T., Kovacs-Bogdan,E., Kamer,K.J.,
            Udeshi,N.D., Carr,S.A., Chaudhuri,D., Clapham,D.E., Li,A.A.,
            Calvo,S.E., Goldberger,O. and Mootha,V.K.
  TITLE     EMRE is an essential component of the mitochondrial calcium
            uniporter complex
  JOURNAL   Science 342 (6164), 1379-1382 (2013)
   PUBMED   24231807
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Raffaello,A., De Stefani,D., Sabbadin,D., Teardo,E., Merli,G.,
            Picard,A., Checchetto,V., Moro,S., Szabo,I. and Rizzuto,R.
  TITLE     The mitochondrial calcium uniporter is a multimer that can include
            a dominant-negative pore-forming subunit
  JOURNAL   EMBO J 32 (17), 2362-2376 (2013)
   PUBMED   23900286
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On Dec 21, 2021 this sequence version replaced XM_006233280.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360181.1648088.1,
                                           SRR26360176.2371749.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN16676784 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-125               JAXUCZ010000002.1  221223690-221223814 c
            126-201             JAXUCZ010000002.1  221175939-221176014 c
            202-372             JAXUCZ010000002.1  221174226-221174396 c
            373-477             JAXUCZ010000002.1  221167755-221167859 c
            478-638             JAXUCZ010000002.1  221157239-221157399 c
            639-842             JAXUCZ010000002.1  221155468-221155671 c
            843-959             JAXUCZ010000002.1  221154250-221154366 c
            960-1168            JAXUCZ010000002.1  221153525-221153733 c
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q43"
     gene            1..1168
                     /gene="Mcub"
                     /gene_synonym="Ccdc109b; RGD1305326"
                     /note="mitochondrial calcium uniporter dominant negative
                     subunit beta"
                     /db_xref="GeneID:295462"
                     /db_xref="RGD:1305326"
     exon            1..125
                     /gene="Mcub"
                     /gene_synonym="Ccdc109b; RGD1305326"
                     /inference="alignment:Splign:2.1.0"
     CDS             30..1037
                     /gene="Mcub"
                     /gene_synonym="Ccdc109b; RGD1305326"
                     /note="coiled-coil domain-containing protein 109B;
                     coiled-coil domain containing 109B; mitochondrial calcium
                     uniporter dominant negative beta subunit"
                     /codon_start=1
                     /product="calcium uniporter regulatory subunit MCUb,
                     mitochondrial"
                     /protein_id="NP_001385602.1"
                     /db_xref="GeneID:295462"
                     /db_xref="RGD:1305326"
                     /translation="MLPSRLCLGRWRLMPIRTHGRWCPRALPPTPQVSGMKLYGNPEY
                     HQALHYGTVTSQDEITVNYRHGLPFVTLTLPSRKERCQFVVKPMLSTVGSFLQDLQNE
                     DKGIKTAAMVTADGSEIPAATLMDTLLMKDFKLVINNLPYDIRRHEKERPSEEHRTQL
                     ENTKCLVHRLFTILHLEEVQKRRERHLMAKIDHLQEQLRPLEQVKAGIEARSEAKTSS
                     LLWAGLALLSVQGGALAWLTWWVYSWDIMEPVTFFLTFANSMVFFAYFIVTRQNYTYE
                     SLRSRQFLQFFHRKSRRQCFDVERYNKLKEDLAEATESLENVRHSLCLRLQGQEVSKK
                     N"
     exon            126..201
                     /gene="Mcub"
                     /gene_synonym="Ccdc109b; RGD1305326"
                     /inference="alignment:Splign:2.1.0"
     exon            202..372
                     /gene="Mcub"
                     /gene_synonym="Ccdc109b; RGD1305326"
                     /inference="alignment:Splign:2.1.0"
     exon            373..477
                     /gene="Mcub"
                     /gene_synonym="Ccdc109b; RGD1305326"
                     /inference="alignment:Splign:2.1.0"
     exon            478..638
                     /gene="Mcub"
                     /gene_synonym="Ccdc109b; RGD1305326"
                     /inference="alignment:Splign:2.1.0"
     exon            639..842
                     /gene="Mcub"
                     /gene_synonym="Ccdc109b; RGD1305326"
                     /inference="alignment:Splign:2.1.0"
     exon            843..959
                     /gene="Mcub"
                     /gene_synonym="Ccdc109b; RGD1305326"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1168
                     /gene="Mcub"
                     /gene_synonym="Ccdc109b; RGD1305326"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tagtgccagg agctcggacc ggcaggagga tgctcccgag ccggctctgt ctgggtcggt
       61 ggcgactgat gccgatccga acccacggcc gctggtgtcc gagggctctg ccacccacgc
      121 cccaggtttc aggtatgaaa ctctatggaa atccagaata ccaccaagca ctccattatg
      181 gaacagtgac gtcacaggac gaaataacag ttaattacag acatggtctt ccctttgtaa
      241 ccctcaccct gccatccaga aaggaacgct gccagttcgt ggtgaagcca atgctgtcga
      301 cagtaggctc gttccttcag gatttacaga atgaagataa aggcatcaaa accgcagcca
      361 tggtcacggc agacggcagc gagattccag ccgcaacatt gatggacact ttgctaatga
      421 aagactttaa gctcgtcatc aacaacctac catatgacat ccggcgtcac gagaaagaaa
      481 ggccaagcga agagcacagg acccagctgg aaaacacgaa gtgcttggtt cacagactct
      541 ttaccatcct gcatttagaa gaggtccaga agagacgaga gcgccatctg atggcgaaga
      601 tcgaccacct gcaggaacag ctgcggcccc tggagcaggt caaagctgga attgaagctc
      661 ggtcagaagc caaaaccagc agtctcctgt gggcaggctt agctctgctg tctgtgcagg
      721 gtggggcact ggcctggctt acttggtggg tgtactcctg ggatatcatg gagccagtta
      781 cgttcttcct tacgtttgcc aattccatgg tcttttttgc atacttcatc gtaactcggc
      841 agaactacac ttacgaatcc cttcggagca ggcagtttct ccagttcttc cacaggaagt
      901 cacgtcggca gtgctttgat gtggaacgat acaacaagct aaaggaggac ctggctgagg
      961 ctacagaatc cctggagaac gtgcgccact ccctctgctt gcgacttcaa ggacaggaag
     1021 tcagcaagaa gaactagcct cacactttta gtcatcaaca ggctatgtgc tggttttata
     1081 gcttaccaag tggatgtttc taaggtcgtg ttcatttctc ttgacgtttt cagatacagt
     1141 ttggttaata aatttgtgtg aaacagaa
//
