LOCUS       NM_001106398            1364 bp    mRNA    linear   ROD 22-MAR-2023
DEFINITION  Rattus norvegicus mitochondrial calcium uniporter (Mcu), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_001106398 XM_001054087 XM_215428
VERSION     NM_001106398.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Hamilton S, Terentyeva R, Perger F, Hernandez Orengo B, Martin B,
            Gorr MW, Belevych AE, Clements RT, Gyorke S and Terentyev D.
  TITLE     MCU overexpression evokes disparate dose-dependent effects on
            mito-ROS and spontaneous Ca2+ release in hypertrophic rat
            cardiomyocytes
  JOURNAL   Am J Physiol Heart Circ Physiol 321 (4), H615-H632 (2021)
   PUBMED   34415186
  REMARK    GeneRIF: MCU overexpression evokes disparate dose-dependent effects
            on mito-ROS and spontaneous Ca(2+) release in hypertrophic rat
            cardiomyocytes.
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Baradaran R, Wang C, Siliciano AF and Long SB.
  TITLE     Cryo-EM structures of fungal and metazoan mitochondrial calcium
            uniporters
  JOURNAL   Nature 559 (7715), 580-584 (2018)
   PUBMED   29995857
REFERENCE   3  (bases 1 to 1364)
  AUTHORS   Zhang K, Yan J, Wang L, Tian X, Zhang T, Guo L, Li B, Wang W and
            Liu X.
  TITLE     The Pyk2/MCU pathway in the rat middle cerebral artery occlusion
            model of ischemic stroke
  JOURNAL   Neurosci Res 131, 52-62 (2018)
   PUBMED   28916471
  REMARK    GeneRIF: This study, identified the role of the Pyk2/MCU pathway in
            the regulation of mitochondrial Ca2+ uptake and apoptotic signaling
            after ischemic stroke.
REFERENCE   4  (bases 1 to 1364)
  AUTHORS   Zheng X, Chen M, Meng X, Chu X, Cai C and Zou F.
  TITLE     Phosphorylation of dynamin-related protein 1 at Ser616 regulates
            mitochondrial fission and is involved in mitochondrial calcium
            uniporter-mediated neutrophil polarization and chemotaxis
  JOURNAL   Mol Immunol 87, 23-32 (2017)
   PUBMED   28388446
REFERENCE   5  (bases 1 to 1364)
  AUTHORS   De La Fuente S, Fernandez-Sanz C, Vail C, Agra EJ, Holmstrom K, Sun
            J, Mishra J, Williams D, Finkel T, Murphy E, Joseph SK, Sheu SS and
            Csordas G.
  TITLE     Strategic Positioning and Biased Activity of the Mitochondrial
            Calcium Uniporter in Cardiac Muscle
  JOURNAL   J Biol Chem 291 (44), 23343-23362 (2016)
   PUBMED   27637331
  REMARK    GeneRIF: MCU and EMRE proteins were mainly targeting to the
            IMM-outer mitochondrial membrane.
REFERENCE   6  (bases 1 to 1364)
  AUTHORS   Yu TN, Hong H, Yang JQ, Gao Q and Xia Q.
  TITLE     [Role of mitochondrial calcium uniporter in cardioprotection
            induced by ischemic postconditioning in isolated rat heart]
  JOURNAL   Zhejiang Da Xue Xue Bao Yi Xue Ban 40 (3), 304-308 (2011)
   PUBMED   21671492
  REMARK    GeneRIF: The inhibition of mitochondrial calcium uniporter is
            involved in the mechanisms of ischemic postconditioning.
REFERENCE   7  (bases 1 to 1364)
  AUTHORS   Xu J, Wang NF, Zhang SZ, Gao Q and Xia Q.
  TITLE     [Mitochondrial calcium uniporter participates in remote
            preconditioning induced cardioprotection in rat hearts subjected to
            ischemia and reperfusion]
  JOURNAL   Zhongguo Ying Yong Sheng Li Xue Za Zhi 25 (1), 50-54 (2009)
   PUBMED   21186615
  REMARK    GeneRIF: Remote preconditioning protects myocardium against
            ischemia and reperfusion injury, possibly by inhibiting
            mitochondrial calcium uniporter opening.
REFERENCE   8  (bases 1 to 1364)
  AUTHORS   Moreau B and Parekh AB.
  TITLE     Ca2+ -dependent inactivation of the mitochondrial Ca2+ uniporter
            involves proton flux through the ATP synthase
  JOURNAL   Curr Biol 18 (11), 855-859 (2008)
   PUBMED   18514515
REFERENCE   9  (bases 1 to 1364)
  AUTHORS   Moreau B, Nelson C and Parekh AB.
  TITLE     Biphasic regulation of mitochondrial Ca2+ uptake by cytosolic Ca2+
            concentration
  JOURNAL   Curr Biol 16 (16), 1672-1677 (2006)
   PUBMED   16920631
REFERENCE   10 (bases 1 to 1364)
  AUTHORS   Ye TM, Zhang SZ and Xia Q.
  TITLE     [Role of mitochondrial calcium uniporter in myocardial
            hypoxia/reoxygenation induced injury]
  JOURNAL   Zhongguo Ying Yong Sheng Li Xue Za Zhi 22 (2), 136-140 (2006)
   PUBMED   21162222
  REMARK    GeneRIF: Inhibition of mitochondrial calcium uniporter may protect
            heart from hypoxia/reoxygenation injury via decreasing the
            production of ROS in heart mitochondria.
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH474016.2.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_215428.4, XM_001054087.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760400,
                              SAMEA5760476 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20q11"
     gene            1..1364
                     /gene="Mcu"
                     /gene_synonym="Ccdc109a"
                     /note="mitochondrial calcium uniporter"
                     /db_xref="GeneID:294560"
                     /db_xref="RGD:1589771"
     exon            1..157
                     /gene="Mcu"
                     /gene_synonym="Ccdc109a"
                     /inference="alignment:Splign:2.1.0"
     CDS             11..1063
                     /gene="Mcu"
                     /gene_synonym="Ccdc109a"
                     /note="coiled-coil domain-containing protein 109A;
                     coiled-coil domain containing 109A; calcium uniporter
                     protein, mitochondrial"
                     /codon_start=1
                     /product="calcium uniporter protein, mitochondrial
                     precursor"
                     /protein_id="NP_001099868.1"
                     /db_xref="GeneID:294560"
                     /db_xref="RGD:1589771"
                     /translation="MAAAAGRSLLLLLCSRGGGGGAGGCGALTAGCFPGLGVSRQRPH
                     QQHRTAHQRPASWQSVGAAYCSTVVPSDDVTVVYQNGLPVISVRLPSRRERCQFTLKP
                     ISDSVGVFLRQLQEEDRGIDRVAIYSPDGVRVAASTGIDLLLLDDFKLVINDLTYHVR
                     PPKRDLLSHENAETLNDVKTLVQQLYTTLCIEQHQLNKERELVERLEDLRQQLAPLEK
                     VRIEISRKAEKRTTLVLWGGLAYMATQFGILARLTWWEYSWDIMEPVTYFITYGSAMA
                     MYAYFVMTRQEYVYPEARDRQYLLFFHKGAKKSRFDLEKYNQLKDAIAQAEMDLKRLR
                     DPLQVHLPLRQIGEKE"
     exon            158..227
                     /gene="Mcu"
                     /gene_synonym="Ccdc109a"
                     /inference="alignment:Splign:2.1.0"
     exon            228..398
                     /gene="Mcu"
                     /gene_synonym="Ccdc109a"
                     /inference="alignment:Splign:2.1.0"
     exon            399..503
                     /gene="Mcu"
                     /gene_synonym="Ccdc109a"
                     /inference="alignment:Splign:2.1.0"
     exon            504..664
                     /gene="Mcu"
                     /gene_synonym="Ccdc109a"
                     /inference="alignment:Splign:2.1.0"
     exon            665..868
                     /gene="Mcu"
                     /gene_synonym="Ccdc109a"
                     /inference="alignment:Splign:2.1.0"
     exon            869..985
                     /gene="Mcu"
                     /gene_synonym="Ccdc109a"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1364
                     /gene="Mcu"
                     /gene_synonym="Ccdc109a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagttgagag atggcggccg ccgcaggtag atcgctcctg ctgctgctct gctcccgggg
       61 cggcggcggg ggcgccggcg gctgcggagc gctgactgcc ggctgctttc ccgggctggg
      121 cgtcagccgc cagcggccgc accagcagca ccggacggca caccagaggc cggcgtcctg
      181 gcagagcgtg ggtgctgcat attgcagtac ggttgtgccc tctgatgacg tgacggtggt
      241 ttatcaaaat ggtttaccgg tgatatctgt gaggctacct tctcggcgag aacgctgcca
      301 gttcacactc aagcctatct cggactccgt cggggtgttt ctccgacaac tgcaagagga
      361 ggatcgggga atcgaccgcg ttgccatcta ttccccagat ggtgttcgag ttgctgcctc
      421 cacggggata gacctcctgc tcctcgatga ctttaagctt gtcattaatg acttaacata
      481 ccacgtacgg ccaccaaaga gagacctcct gagccatgaa aacgcagaga ccctgaacga
      541 tgtgaagacg ctggtccagc agctctacac cacactgtgc attgagcagc atcagcttaa
      601 taaggagcgg gagctcgtgg agaggttaga ggacctcaga cagcagctgg cccccctgga
      661 gaaggtacgg attgaaatta gcaggaaagc ggagaagagg accactctgg tgctatgggg
      721 aggccttgcc tacatggcca cacagtttgg cattttggcc cggcttacct ggtgggaata
      781 ctcctgggac atcatggaac cggtcaccta cttcatcacg tatgggagcg ccatggccat
      841 gtatgcgtat tttgtaatga cgcgccagga atatgtttat cctgaagcca gagacagaca
      901 atacttatta tttttccata aaggagccaa aaagtctcgt ttcgacctag agaaatacaa
      961 tcaactcaag gatgcaattg ctcaggcaga aatggatctt aagagactga gagacccgct
     1021 acaagtacat ctgcccctcc gacagatcgg agaaaaggaa tgatcagaga tgagcgtgaa
     1081 tcccggcagt cagcgcccct gtttgtaact cacgcctttt taattaaagc atggcaggtg
     1141 gatgctgggg ccgtgtgggg tagagggttt ttacctttaa ttctaaaaac aaaacacaaa
     1201 aaggatctta ggggaaaggg gatcttaaat gccgaggatc aggcttcgtg gaatccaagt
     1261 gcaaagggct ttgcgaatct cgtcttgagc acccgattct gggtgagagt gatgacgtca
     1321 cagctcgagg gggtgctggg tttgcttcgt gcatctttgc aggg
//
