LOCUS       NM_172044                978 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus mast cell protease 1 (Mcpt1), mRNA.
ACCESSION   NM_172044
VERSION     NM_172044.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Fu,Z., Thorpe,M. and Hellman,L.
  TITLE     rMCP-2, the Major Rat Mucosal Mast Cell Protease, an Analysis of
            Its Extended Cleavage Specificity and Its Potential Role in
            Regulating Intestinal Permeability by the Cleavage of Cell Adhesion
            and Junction Proteins
  JOURNAL   PLoS One 10 (6), e0131720 (2015)
   PUBMED   26114959
  REMARK    GeneRIF: Phage display analysis showed that rMCP-2 is a chymase
            with a specificity similar to the corresponding enzyme in mice,
            mMCP-1, with a preference for Phe or Tyr in the P1 position, and a
            general preference for aliphatic amino acids both upstream and
            downstream of the cleavage site.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  AUTHORS   Abril-Gil,M., Garcia-Just,A., Perez-Cano,F.J., Franch,A. and
            Castell,M.
  TITLE     Development and characterization of an effective food allergy model
            in Brown Norway rats
  JOURNAL   PLoS One 10 (4), e0125314 (2015)
   PUBMED   25923134
  REMARK    GeneRIF: These results show both an effective and relatively rapid
            model of FA assessed by means of specific antibody titres and the
            high production of RMCP-II and its intestinal gene expression
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 978)
  AUTHORS   Blum,L.K., Thrasher,S.M., Gagliardo,L.F., Fabre,V. and
            Appleton,J.A.
  TITLE     Expulsion of secondary Trichinella spiralis infection in rats
            occurs independently of mucosal mast cell release of mast cell
            protease II
  JOURNAL   J Immunol 183 (9), 5816-5822 (2009)
   PUBMED   19812197
  REMARK    GeneRIF: Rapid expulsion of Trichinella spiralis first-stage larvae
            from rat intestine can occur in the absence of either intestinal
            mastocytosis or mast cell protease II release.
REFERENCE   4  (bases 1 to 978)
  AUTHORS   Remington,S.J., Woodbury,R.G., Reynolds,R.A., Matthews,B.W. and
            Neurath,H.
  TITLE     The structure of rat mast cell protease II at 1.9-A resolution
  JOURNAL   Biochemistry 27 (21), 8097-8105 (1988)
   PUBMED   3233198
REFERENCE   5  (bases 1 to 978)
  AUTHORS   Benfey,P.N., Yin,F.H. and Leder,P.
  TITLE     Cloning of the mast cell protease, RMCP II. Evidence for
            cell-specific expression and a multi-gene family
  JOURNAL   J Biol Chem 262 (11), 5377-5384 (1987)
   PUBMED   3549719
REFERENCE   6  (bases 1 to 978)
  AUTHORS   Woodbury,R.G., Katunuma,N., Kobayashi,K., Titani,K., Neurath,H.,
            Anderson,W.F. and Matthews,B.W.
  TITLE     Covalent structure of a group-specific protease from rat small
            intestine. Appendix: crystallographic data for a group specific
            protease from rat intestine
  JOURNAL   Biochemistry 17 (5), 811-819 (1978)
   PUBMED   629933
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000015.1.
            
            On Sep 13, 2022 this sequence version replaced NM_172044.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CF110755.1, SRR26643291.36942.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-86                JAXUCZ010000015.1  33841606-33841691
            87-237              JAXUCZ010000015.1  33842199-33842349
            238-373             JAXUCZ010000015.1  33842867-33843002
            374-628             JAXUCZ010000015.1  33843206-33843460
            629-978             JAXUCZ010000015.1  33843825-33844174
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p12"
     gene            1..978
                     /gene="Mcpt1"
                     /gene_synonym="rMCP-1; rMCP-2; rMCP-I; rMCP-II"
                     /note="mast cell protease 1"
                     /db_xref="GeneID:29266"
                     /db_xref="RGD:621058"
     exon            1..86
                     /gene="Mcpt1"
                     /gene_synonym="rMCP-1; rMCP-2; rMCP-I; rMCP-II"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..775
                     /gene="Mcpt1"
                     /gene_synonym="rMCP-1; rMCP-2; rMCP-I; rMCP-II"
                     /EC_number="3.4.21.39"
                     /note="group-specific protease; mast cell peptidase 1;
                     mast cell protease I; mast cell protease II; mast cell
                     peptidase 2"
                     /codon_start=1
                     /product="mast cell protease 1 precursor"
                     /protein_id="NP_742041.1"
                     /db_xref="GeneID:29266"
                     /db_xref="RGD:621058"
                     /translation="MQALLFLMALLLPSGAGAEEIIGGVESIPHSRPYMAHLDIVTEK
                     GLRVICGGFLISRQFVLTAAHCKGREITVILGAHDVRKRESTQQKIKVEKQIIHESYN
                     SVPNLHDIMLLKLEKKVELTPAVNVVPLPSPSDFIHPGAMCWAAGWGKTGVRDPTSYT
                     LREVELRIMDEKACVDYRYYEYKFQVCVGSPTTLRAAFMGDSGGPLLCAGVAHGIVSY
                     GHPDAKPPAIFTRVSTYVPWINAVINTSS"
     exon            87..237
                     /gene="Mcpt1"
                     /gene_synonym="rMCP-1; rMCP-2; rMCP-I; rMCP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            238..373
                     /gene="Mcpt1"
                     /gene_synonym="rMCP-1; rMCP-2; rMCP-I; rMCP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            374..628
                     /gene="Mcpt1"
                     /gene_synonym="rMCP-1; rMCP-2; rMCP-I; rMCP-II"
                     /inference="alignment:Splign:2.1.0"
     exon            629..978
                     /gene="Mcpt1"
                     /gene_synonym="rMCP-1; rMCP-2; rMCP-I; rMCP-II"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaagctcacc ccaggctgca ccactggcac aatgcaggcc ctactattcc tgatggctct
       61 tctcttgcct tctggagctg gagctgagga gattatcggt ggtgtggagt ctattcctca
      121 ctcccgccct tacatggccc atctggacat cgtcactgag aaaggtttaa gggtcatctg
      181 tggtgggttt ctcataagcc gtcaatttgt gctgactgct gcacattgta aaggaagaga
      241 aatcacagtc atccttggag cccatgatgt gagaaagcga gaatccacac agcagaagat
      301 aaaagtcgaa aaacaaatca ttcacgaaag ttacaactcc gttcccaatc ttcatgacat
      361 catgttactg aagcttgaaa aaaaagttga gttgactcct gctgtgaatg tagttcctct
      421 gcccagtccc tctgacttta tccaccctgg ggcaatgtgt tgggcagctg gatgggggaa
      481 aactggagtt agagatccta cctcgtatac actgagagag gttgaactga gaatcatgga
      541 tgaaaaggcc tgtgtggact acaggtatta tgaatacaaa ttccaggtct gcgtgggcag
      601 tcccacaact ttaagagcag cattcatggg agactctggc ggaccgctac tgtgtgctgg
      661 tgtggctcat ggtattgtat cttatgggca tccagatgca aagccccctg caatcttcac
      721 ccgagtctcc acatatgtgc cctggattaa tgcagtcatt aatacaagta gctgaaaagc
      781 ctgacctgct tgagacaccc tccaagcctg agttcatctg gtaacttttg gttttaacac
      841 agcctgtttc cagcatgtgt ctcagcctgt ccctagcctg tctccagctg tctctaagag
      901 gccctcaaag atatacaagt ctgtgatgat ggattgttcc ctgtaaagca cttcaataaa
      961 gacataactt ccaacagc
//
