LOCUS       NM_001106514            3226 bp    mRNA    linear   ROD 17-JUL-2024
DEFINITION  Rattus norvegicus minichromosome maintenance 8 homologous
            recombination repair factor (Mcm8), transcript variant 2, mRNA.
ACCESSION   NM_001106514 XM_001079873 XM_215825
VERSION     NM_001106514.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Traver,S., Coulombe,P., Peiffer,I., Hutchins,J.R., Kitzmann,M.,
            Latreille,D. and Mechali,M.
  TITLE     MCM9 Is Required for Mammalian DNA Mismatch Repair
  JOURNAL   Mol Cell 59 (5), 831-839 (2015)
   PUBMED   26300262
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Lee,K.Y., Im,J.S., Shibata,E., Park,J., Handa,N.,
            Kowalczykowski,S.C. and Dutta,A.
  TITLE     MCM8-9 complex promotes resection of double-strand break ends by
            MRE11-RAD50-NBS1 complex
  JOURNAL   Nat Commun 6, 7744 (2015)
   PUBMED   26215093
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3226)
  AUTHORS   Park,J., Long,D.T., Lee,K.Y., Abbas,T., Shibata,E., Negishi,M.,
            Luo,Y., Schimenti,J.C., Gambus,A., Walter,J.C. and Dutta,A.
  TITLE     The MCM8-MCM9 complex promotes RAD51 recruitment at DNA damage
            sites to facilitate homologous recombination
  JOURNAL   Mol Cell Biol 33 (8), 1632-1644 (2013)
   PUBMED   23401855
REFERENCE   4  (bases 1 to 3226)
  AUTHORS   Lutzmann,M., Grey,C., Traver,S., Ganier,O., Maya-Mendoza,A.,
            Ranisavljevic,N., Bernex,F., Nishiyama,A., Montel,N., Gavois,E.,
            Forichon,L., de Massy,B. and Mechali,M.
  TITLE     MCM8- and MCM9-deficient mice reveal gametogenesis defects and
            genome instability due to impaired homologous recombination
  JOURNAL   Mol Cell 47 (4), 523-534 (2012)
   PUBMED   22771120
REFERENCE   5  (bases 1 to 3226)
  AUTHORS   Nishimura,K., Ishiai,M., Horikawa,K., Fukagawa,T., Takata,M.,
            Takisawa,H. and Kanemaki,M.T.
  TITLE     Mcm8 and Mcm9 form a complex that functions in homologous
            recombination repair induced by DNA interstrand crosslinks
  JOURNAL   Mol Cell 47 (4), 511-522 (2012)
   PUBMED   22771115
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473949.1.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_001079873.1, XM_215825.4.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132296,
                              SAMD00132304 [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q36"
     gene            1..3226
                     /gene="Mcm8"
                     /note="minichromosome maintenance 8 homologous
                     recombination repair factor"
                     /db_xref="GeneID:296178"
                     /db_xref="RGD:1305218"
     exon            1..266
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            267..429
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     CDS             291..2783
                     /gene="Mcm8"
                     /EC_number="3.6.4.12"
                     /note="isoform a is encoded by transcript variant 2;
                     minichromosome maintenance deficient 8; DNA replication
                     licensing factor MCM8; DNA helicase MCM8; minichromosome
                     maintenance complex component 8"
                     /codon_start=1
                     /product="DNA helicase MCM8 isoform a"
                     /protein_id="NP_001099984.1"
                     /db_xref="GeneID:296178"
                     /db_xref="RGD:1305218"
                     /translation="MSGAYRGRGFGRGRFQNWKRGRGGGNFSGRWRDRTDLSKAAGNH
                     ASEQASQPLLQQSTLDQFIPYKGWKLYFSEVYSNNSPLTEKIQAFEKFFTRHIDLYDK
                     DEIERKGSILVDFKELTKDNEITNLIPDIENALRDAPEKTLACMGLAIHQVLTKDLER
                     HAAELQAQEGLCNGGGTMVNVPHIYARVYNYEPLTHLKNIRATCYGKYISIRGTVVRV
                     SNIKPLCTKMAFQCAACGEIQSFPLPDGKYNLPTKCPVPACRGRSFTPLRSSPLTVTM
                     DWQLIKIQELMSDAQREAGRIPRTIECELVHDLVDSCVPGDTVTVTGIVKVSNSEEGS
                     RSKNDKCMFLLYIEANSVSNSKGQKAQTAEDGCKHGTLMEFSLKDLYAIQEIQAEENL
                     LKLIVNSLCPVIFGHELVKAGLMLALFGGSQKYADDKNRIPIRGDPHVLIVGDPGLGK
                     SQMLQAACNVAPRGVYVCGNTATSSGLTVTLSKDSSSGDFALEAGALVLGDQGICGID
                     EFDKMGNQHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKARTVSEN
                     LKMGSALLSRFDLVFILLDTPNEQHDHLLSEHVIAIRAGKQRAVSSATVSRVLSQDSN
                     TSVLEVVSEKPLSERLKVAPGEKTDPIPHQLLRKYIGYARQYVHPRLSTEAAQALQDF
                     YLELRKQSQRVGSSPITTRQLESLIRLTEARARLELREEATKEDAEDIIEIMKHSMLG
                     TYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNSIAERTYNNIFQFHQLRQIAKELN
                     IQVADFENFIGSLNDQGYLLKKGPKIYQLQTM"
     exon            430..513
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            514..596
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            597..746
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            747..850
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            851..1049
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1135
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1287
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1483
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1514
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1655
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1797
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            1798..1993
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            1994..2213
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            2214..2423
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            2424..2500
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            2501..2690
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
     exon            2691..3226
                     /gene="Mcm8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cttcgagcga ggtcggagtt taccctgtgg tgttgtgccg ggccttcgcc gccctgcgtc
       61 tcgcctcggg cgactccgaa gacggaatgt ctgcgtaacc ctgcgtggga ctgcgtaccg
      121 cctatggctc tgtggctggc tgggagactc gggggactcc tgaaagtagg acgcgagaaa
      181 aacgcggcga gaatgaagtc agggattgcg gggggctttt ctcaggctgc ttccctatgt
      241 agttgttgga agtgcctccg ttctcaaagt gctgttgact ctcagaagac atgagtggtg
      301 catacagagg cagaggtttt ggacgaggac gattccaaaa ctggaaaaga ggaagaggtg
      361 gtgggaactt ctcaggaagg tggagagaca gaactgacct gagtaaagct gcaggaaatc
      421 atgcttctga acaagcctca caacctttac ttcaacagtc aacattggat cagtttatac
      481 cgtataaggg ctggaagctt tacttctctg aagtttacag taataactct cctcttactg
      541 agaagatcca agcatttgag aaatttttca caaggcatat cgacttatat gataaggatg
      601 aaatagaaag aaagggaagc atcttggtgg attttaaaga actgacaaaa gacaatgaaa
      661 taactaactt gataccagat atagaaaatg cactaagaga tgcccctgag aaaacactgg
      721 catgtatggg actggcaata catcaggtat taactaagga ccttgaaagg cacgcagctg
      781 aattacaagc tcaagagggg ttgtgtaatg gtggaggaac catggtaaat gtgccacata
      841 tttatgcgag agtgtacaac tatgagccct taacacacct caagaacatc cgagcaactt
      901 gctatgggaa gtacatctct ataagaggga cagtggttcg agtcagtaac ataaagcctc
      961 tgtgcaccaa aatggctttt cagtgtgctg catgtggaga gattcagagc tttcctcttc
     1021 cggatggaaa atacaatctt ccaacaaagt gtcctgtgcc tgcgtgtcga gggaggtcat
     1081 ttacgccatt gcgcagttct cctctcaccg tcacaatgga ctggcagttg atcaaaatcc
     1141 aggagctgat gtctgatgca cagagagaag ctggtcggat ccctcgaacc atagaatgtg
     1201 aacttgttca tgatcttgta gatagctgtg tcccaggaga tacggtgact gttactggaa
     1261 ttgtcaaagt ttcaaatagt gaagaaggtt ctcgatctaa gaatgataag tgcatgttcc
     1321 ttttgtacat tgaagcaaac tctgttagca acagcaaagg acagaaagcc cagactgcag
     1381 aggatggttg caagcatgga acactgatgg agttctccct taaagacctg tatgccatcc
     1441 aagagatcca ggctgaagag aacctgctca agctcattgt caactcactt tgtcctgtca
     1501 tttttggtca tgaacttgtt aaagcaggac tgatgttagc gctatttggc ggtagccaga
     1561 agtacgcaga tgacaaaaac aggattccta ttcgaggaga cccacacgtc ctgattgttg
     1621 gagatccagg cttggggaag agtcagatgc tgcaggcagc atgcaacgtg gcgccacgtg
     1681 gtgtgtatgt ttgtggaaat acggccacca gctctggtct gacggttact ctttcaaaag
     1741 acagttcctc aggagacttc gctttggaag ctggtgccct ggtacttggt gaccaaggca
     1801 tttgtggaat agatgaattt gataaaatgg ggaatcagca tcaagccttg ttagaagcca
     1861 tggaacagca gagcattagc cttgctaagg ccggtgtggt ttgcagcctc cctgcaagaa
     1921 cctccattat tgctgcggca aatccagtcg gaggacacta caataaagcg aggacagttt
     1981 ctgagaattt aaagatgggg agtgccctgc tgtccaggtt cgatttggtc tttatcttgt
     2041 tagatacacc aaatgaacag cacgaccact tactttctga acatgtgatc gcaataagag
     2101 ctgggaagca gagagcggtt agcagtgcca cagtctctcg tgtgctcagt caagactcaa
     2161 atacttctgt acttgaagtg gtttccgaga aaccgttatc agaaagacta aaggtggctc
     2221 ctggagaaaa gacagatccg attccacatc agctgctgag gaagtacata ggctatgcac
     2281 ggcagtatgt ccatccaagg ttatccacgg aagctgctca ggctcttcag gatttctacc
     2341 tggagctgcg caagcagagc cagcgcgtgg gcagctcgcc catcaccact cggcagctgg
     2401 aatctttgat ccgtctgacg gaggcacgag caaggttaga attgagagag gaagcaacta
     2461 aagaagacgc tgaagatata attgaaatta tgaagcatag catgctagga acttactcag
     2521 atgaatttgg aaacctggac tttgagcgat cccagcatgg ctctggaatg agcaacaggt
     2581 caacagctaa aagatttatt tctgctctca acagcattgc tgaaagaacg tataacaaca
     2641 tatttcaatt tcatcaactt cgtcagattg ctaaagagct aaacattcag gttgctgact
     2701 ttgaaaactt cattggatca ctgaatgacc agggttatct cctgaaaaaa ggcccaaaga
     2761 tttatcaact tcaaactatg tgaaagactt tcctatgtca gggcctcctg tagttcaaaa
     2821 aatttcagaa tcatcttaaa tataaaactg aaaataaaag ttctgaaaaa tgacacaact
     2881 cataacagaa aaaaaggtat atccataatt taccagaaga atacgaagag taaattattt
     2941 ccactatttt atctaaataa cataagcatt atttatgctt ttggcttata tttttattag
     3001 tgctttttag ttttgtatat cagatgtcta gcaggcattc agataatata taaattagta
     3061 tgtgtttaag ttttatttaa aacattaaac tttgaatctc acaaattgtt aaggtcacaa
     3121 aaactcttca tttgtttcta actttatggt tatgggcagt gggtctgact tggcttctta
     3181 tttgaaagtg gtggaaactt attgacttaa ttagaagtct tttctg
//
