LOCUS       XM_006253424            5564 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus MCF.2 cell line derived transforming
            sequence-like (Mcf2l), transcript variant X10, mRNA.
ACCESSION   XM_006253424
VERSION     XM_006253424.4
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051351.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Feb 22, 2024 this sequence was replaced by
            XM_006253424.5.
            
            On Jan 21, 2021 this sequence version replaced XM_006253424.3.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..5564
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /note="MCF.2 cell line derived transforming sequence-like;
                     Derived by automated computational analysis using gene
                     prediction method: Gnomon. Supporting evidence includes
                     similarity to: 14 ESTs, 11 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 41 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:117020"
                     /db_xref="RGD:619782"
     CDS             421..3960
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /codon_start=1
                     /product="guanine nucleotide exchange factor DBS isoform
                     X10"
                     /protein_id="XP_006253486.1"
                     /db_xref="GeneID:117020"
                     /db_xref="RGD:619782"
                     /translation="MTLSLISVLSQDVTALWLLLKTSADEIMHQDIVPLCAADIQEQL
                     KKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILV
                     IDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKMKV
                     PVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQMLQA
                     FGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALTEGNSILESLREPLAESIV
                     HSVNQDQLDNQATVKRLLTQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKTA
                     LDSMSQKIAAFTDVGNSLAHVQHLLKDLTTFEEKSSVAVDKARALSLEGQQLIENRHY
                     AVDSIHPKCEELQHLCDHFASEVTRRRDLLSKSLELHSLLETSMKWSDEGIFLLASQP
                     VDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQKQ
                     ESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEGNA
                     LRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCVLE
                     GYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRC
                     FLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQRI
                     TKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGK
                     LLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPS
                     YSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKVLT
                     SQLQACREASQHRALEQSHSLPLPTPASTSPTKGSTRNVKKLEDRKTDPLCLEGCVSS
                     SLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKS
                     DPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPRKLVPGKYTVLM
                     DGEKGGSDTLAMRSGDMVEVVEEGTEGLWYVRDLTSSKEGWVPASSLATLLGKSSSAQ
                     CLSSSDSSPGSAVLSNSSSCSEGYPTPFSELQG"
ORIGIN      
        1 ctaaggctta ggtccctgtg ccctgctgtc cctgtggatg gaacctgact gaggtggggc
       61 ccctgcaggg agggtccaga caaacaacaa ggcgcctggc tctgcagcca gcaggactca
      121 gcggctgcca ggctctcaac agttaaccat ctgcagcagg ataagtgagc tgattccagc
      181 tccttaatca ggacattgaa tccgaatctg gggccctgca aggagcagca gccgctgctg
      241 tgctgcgcct cagcctgtgg cactcagagg acggcaggac tggactgagc agcgaagcca
      301 gggagacagt atccccactc agccgcccaa gatggagctc cactggcttc ctttgtgtga
      361 cccaagccgg cttgccagca aacaagtcat tgggtcctga ccagtgtgga gggtgacagc
      421 atgactttga gcctcatctc cgtgctgtcc caggacgtca cagccctgtg gctcctgctg
      481 aagacaagtg cagatgaaat catgcaccag gacatcgtcc cactctgtgc cgctgacatc
      541 caggagcagc tgaagaagcg ctttgcttac ctgtctggtg ggcggggaca ggacggaagc
      601 ccagtcatca ccttcccaga ctacccagcc ttcagcgaga tcccggacaa ggagttccag
      661 aatgtcatga cctatctcac cagcatcccc agtttgcaag atgctggcat cgggttcatc
      721 ttggtcatag accgaagaca ggacaaatgg acttctgtga aggcgtcagt cctgcgaata
      781 gccgcatcat tcccagccaa cctgcagctc gtcctcgttc tccgacccac gggcttcttc
      841 cagaggactc tctcggacat agccttcaag ttcaacagag atgagtttaa gatgaaagta
      901 ccggtcatga tgctaagctc agtgccagaa ctacatggct acattgacaa gtcacagctg
      961 accgaggacc ttggggggac cctggactac tgccactcca ggtggctgtg ccaccgcaca
     1021 gcgattgaaa gcttcgccct gatggtgaag cagacagccc agatgctgca ggcctttggg
     1081 accgagctgg ccgagactga gctgcccaac gacgtccagt caaccagcct ggtgctcagc
     1141 gcacacacag agaagaaggc taaagtgaag gaggatctgc agctggcgct gacggagggg
     1201 aacagcatcc ttgagagcct cagggagccg ctggctgaga gcatagtcca cagtgtgaac
     1261 caggaccagc tggacaatca ggccactgtg aagaggctcc tgacccagct gaatgagacc
     1321 gaggctgcct ttgacgagtt ctgggcaaag catcagcaga agctggaaca gtgcctacag
     1381 ctgcgacatt ttgaacaagg tttccgggag gtcaaaaccg ccttggattc catgtcccag
     1441 aagatagccg cgttcacaga tgttggcaac agcctggccc atgtgcagca cctcctgaag
     1501 gacttgacca cctttgagga gaagtccagc gtggccgtgg acaaggccag agccctgtcc
     1561 ctggaggggc agcagctgat tgagaaccgg cactatgctg tagactctat ccatccgaag
     1621 tgtgaggaac tccagcacct ctgtgaccac ttcgcctctg aggtcaccag gaggcgggac
     1681 cttctcagca agtccttgga gctgcatagc ctcctggaga cgtccatgaa gtggagcgac
     1741 gagggcatct tcctgctggc ctctcagcct gtggacaaat gccaatctca ggatggtgcg
     1801 gaggcagccc tgcaggagat cgagaagttt ctggagaccg gtgcagaaaa caagattcag
     1861 gagctcaata agatttacaa ggaatatgaa tgcatcctca accaggacct tctggaacac
     1921 gtgcaaaaag tctttcagaa gcaagagagc accgaggaga tgttccaccg caggcaggct
     1981 agcctgaaga aactggcagc caagcagaca cggcctgtgc agcccgtggc cccccgacca
     2041 gaggcgctaa ccaagtcccc ctccccctcg ccaggctcct ggcgaagctc tgagaactcc
     2101 agctctgagg gcaacgccct ccgcagaggg ccctacagga gagccaagag cgaaatgagt
     2161 gagccccggc agggccgcac cagctccaca ggagaagagg aggagagcct ggccatcctg
     2221 cgcagacacg tgatgaatga acttctggac acggagcggg cctacgtgga ggagctgctc
     2281 tgtgtcttag agggctatgc tgcagagatg gacaacccct tgatggcaca cctcatctca
     2341 acaggcctgc aaaacaagaa gaacattctg tttggaaaca tggaggaaat ctatcacttc
     2401 cacaacagaa tattcctgcg ggagctggag agctgcatcg actgcccaga gctggtgggg
     2461 agatgctttc tggagaggat ggaggagttc cagatctatg agaagtactg tcagaacaag
     2521 ccacgctccg agagcctgtg gagacagtgc tctgactgtc ccttcttcca ggagtgccag
     2581 aagaagctgg accacaagct gagcctcgac tcctacctgc tgaagcctgt gcagaggata
     2641 accaagtacc agctgctgct caaggaaatg ctgaagtaca gcaagcactg tgaaggggcc
     2701 gaggacctgc aagaggcact gagctccatc ctgggcatcc tcaaggcagt gaacgactcc
     2761 atgcacctca tagccatcac tggctatgat ggaaaccttg gggacctggg gaagctgctg
     2821 atgcaaggct ccttcagcgt gtggacggac cacaagaagg gccacaccaa ggtgaaggag
     2881 ctagccaggt tcaagcccat gcagcggcac ctcttccttc acgagaaggc cgtcctcttc
     2941 tgcaagaaga gggaggagaa tggggagggg tacgagaagg ctccttccta cagctacaag
     3001 cagtccttga atatgactgc tgttggcatc acagagaatg tgaagggtga caccaagaag
     3061 ttcgagatct ggtacaacgc aagggaggag gtttacatca ttcaggcacc aactcccgag
     3121 attaaagcag cgtgggtgaa tgagattcgg aaggtgctga ccagccaact gcaggcctgc
     3181 cgggaagcca gccagcaccg agccctggag cagtcccaca gccttccctt gcccacgcca
     3241 gccagcacca gtcccacaaa agggagcaca agaaatgtca agaagctcga agatcggaag
     3301 acggacccgc tctgcctgga aggctgcgtg agctcatcat taccaaagcc ccccgagaag
     3361 ggcaaagacg atgcggtccc tagttctacc tctgaaagct ctgcgctttc cagaaagcgc
     3421 ttcacgctgc agggcttggc taacctcaaa ggtcagaaag cctctccgac cagtcctgac
     3481 aaaaaagcta agcggcatga agtaaagagc gacccgactc cctttggttt gcgaggctgg
     3541 agcaaaacat cccactcact ggaggcccct gaggaggacg gaggctggtc cagtgctgag
     3601 gagctcatca attcatccga tgctgaggaa gacggaggag tgggccctag gaagctggtt
     3661 ccaggtaaat acacagtgct gatggatggt gagaaagggg gctctgacac ccttgccatg
     3721 aggagtggag atatggtgga ggttgtggaa gaaggaactg agggcctctg gtatgttcgg
     3781 gacctgacca gcagcaagga gggatgggtg ccagccagca gcctagccac cctcctgggc
     3841 aaatccagct cagcccagtg tctgagcagc tcagactcca gcccagggtc agccgtgctg
     3901 agcaactcat ccagctgcag cgagggctac cctactccct tctccgagct ccaggggtag
     3961 tggccaggcc tctgtggcag gcgctcaagg ccaccgtgca gtagaccagc atgcaaatct
     4021 ctaagttttt tctttttgct tgggcttgct tagtttggga ggggatggtg ctccaagata
     4081 tctggaaaca ggaggaccta tcaaccatgg gcttcagagg acactggggc cttggaacca
     4141 ggactctgct gcatatgcag aaggcaggag acaacagttc accaccaaag cttcttcaga
     4201 gactccaggg tggacagagg cagggctatg ggtcaggaga ccccttgcag aaagatggga
     4261 gacggccctt ttccacacat gcacagccag cagcccctgc tatacagcct ctccacggtg
     4321 tcctggcctc tctggtgctt tctacgggaa catcatcctg aggaagccac ttctaaccgg
     4381 agtaaagagt ttgctaccaa cagcctaaga aggggatcaa gggactcttg tcttttataa
     4441 gtttttgact ttttttttaa aaaaaacaaa caaacaggga ttaatcccat ggccattttt
     4501 ctgtggtact ctggcctcaa atcctaaacc aagaatcact gtgtttacat gaaatgacaa
     4561 tttgatactg tatttgatat aaaactattt tttgttactg gggtttacat caaagcacgt
     4621 tgtttataca actgactgat ttctggggca ctctcactga gacctggcag cacaggtgct
     4681 ggaaggcctg gtaagccagg gtcctgtcct cctggtaagc ccagggcgtg tcctcttagc
     4741 cagggggcgt gtcctcctgg ttagccaggg aggggcgtgt cctcctggta agacaggggg
     4801 cgtgtcctct tagccagggg gtgtgtcctc ctggttagcc agggaggggc gtgtcctcca
     4861 ctaaaagcgt tggaaatcac tgagagtctt gcatctgcat aggtttcatt ttcacaagaa
     4921 atgtaaagta taatttaaaa tattagcctt cttttctaca atataaagga gactctctgt
     4981 ttttttaaaa gaacatcatt aaattaggat atttttatta tttttaaaga aaatgtaaaa
     5041 ctccatgctg tcagccatcc tcagcaactc aggcacaggc tgcctctcca tgtcagggca
     5101 ggcccgactt cccctccccg agtcccaccc acctctgccc tacagcagga ggggctgcaa
     5161 ctgagctcat gggggggggg ggcctgcagc ctccattcaa gtcaggcaga actgccacag
     5221 actcttgttt ccccctttta tgtgtgtgtt tcagaaatcg attctgttct ttggaaactc
     5281 tgtgccgagt agggcaggta tttttatact tgtgtatata tgttcacgtg tgtacatatg
     5341 atctggtgta aaggatgtat ttatggtctc acgctgctga acatgtgtca ctttacggtc
     5401 cctccctgtc tcctcacccc gctgccatgg gaggccaccg aggctcgttc accactgtat
     5461 gtccaggaaa accaccctct tccctgccct atggcttgca aagccattac tggctacacc
     5521 ttcagagaat ggtaattaaa taaaaacgtg caaagactat cgca
//
