LOCUS       NM_001413769            5346 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus MCF.2 cell line derived transforming
            sequence-like (Mcf2l), transcript variant 2, mRNA.
ACCESSION   NM_001413769 XM_039094161
VERSION     NM_001413769.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Bohm,D., Keller,K., Pieter,J., Boehm,N., Wolters,D., Siggelkow,W.,
            Lebrecht,A., Schmidt,M., Kolbl,H., Pfeiffer,N. and Grus,F.H.
  TITLE     Comparison of tear protein levels in breast cancer patients and
            healthy controls using a de novo proteomic approach
  JOURNAL   Oncol Rep 28 (2), 429-438 (2012)
   PUBMED   22664934
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Kostenko,E.V., Olabisi,O.O., Sahay,S., Rodriguez,P.L. and
            Whitehead,I.P.
  TITLE     Ccpg1, a novel scaffold protein that regulates the activity of the
            Rho guanine nucleotide exchange factor Dbs
  JOURNAL   Mol Cell Biol 26 (23), 8964-8975 (2006)
   PUBMED   17000758
REFERENCE   3  (bases 1 to 5346)
  AUTHORS   Ueda,S., Kataoka,T. and Satoh,T.
  TITLE     Role of the Sec14-like domain of Dbl family exchange factors in the
            regulation of Rho family GTPases in different subcellular sites
  JOURNAL   Cell Signal 16 (8), 899-906 (2004)
   PUBMED   15157669
REFERENCE   4  (bases 1 to 5346)
  AUTHORS   Rossman,K.L., Cheng,L., Mahon,G.M., Rojas,R.J., Snyder,J.T.,
            Whitehead,I.P. and Sondek,J.
  TITLE     Multifunctional roles for the PH domain of Dbs in regulating Rho
            GTPase activation
  JOURNAL   J Biol Chem 278 (20), 18393-18400 (2003)
   PUBMED   12637522
REFERENCE   5  (bases 1 to 5346)
  AUTHORS   Horii,Y., Beeler,J.F., Sakaguchi,K., Tachibana,M. and Miki,T.
  TITLE     A novel oncogene, ost, encodes a guanine nucleotide exchange factor
            that potentially links Rho and Rac signaling pathways
  JOURNAL   EMBO J 13 (20), 4776-4786 (1994)
   PUBMED   7957046
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000016.1.
            
            On Nov 8, 2022 this sequence version replaced XM_039094161.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360176.2251050.1,
                                           SRR26360194.71027.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-144               JAXUCZ010000016.1  83314619-83314762   c
            145-228             JAXUCZ010000016.1  83267188-83267271   c
            229-343             JAXUCZ010000016.1  83262140-83262254   c
            344-434             JAXUCZ010000016.1  83249388-83249478   c
            435-554             JAXUCZ010000016.1  83240294-83240413   c
            555-671             JAXUCZ010000016.1  83237659-83237775   c
            672-821             JAXUCZ010000016.1  83237000-83237149   c
            822-946             JAXUCZ010000016.1  83235698-83235822   c
            947-1061            JAXUCZ010000016.1  83233502-83233616   c
            1062-1181           JAXUCZ010000016.1  83230331-83230450   c
            1182-1373           JAXUCZ010000016.1  83229715-83229906   c
            1374-1565           JAXUCZ010000016.1  83229163-83229354   c
            1566-1725           JAXUCZ010000016.1  83228337-83228496   c
            1726-1799           JAXUCZ010000016.1  83227393-83227466   c
            1800-1876           JAXUCZ010000016.1  83227089-83227165   c
            1877-1943           JAXUCZ010000016.1  83225399-83225465   c
            1944-2059           JAXUCZ010000016.1  83224422-83224537   c
            2060-2129           JAXUCZ010000016.1  83223458-83223527   c
            2130-2222           JAXUCZ010000016.1  83220802-83220894   c
            2223-2315           JAXUCZ010000016.1  83220596-83220688   c
            2316-2441           JAXUCZ010000016.1  83219854-83219979   c
            2442-2663           JAXUCZ010000016.1  83218794-83219015   c
            2664-2756           JAXUCZ010000016.1  83218509-83218601   c
            2757-2835           JAXUCZ010000016.1  83217406-83217484   c
            2836-2902           JAXUCZ010000016.1  83217213-83217279   c
            2903-3018           JAXUCZ010000016.1  83216682-83216797   c
            3019-3111           JAXUCZ010000016.1  83216337-83216429   c
            3112-3186           JAXUCZ010000016.1  83215480-83215554   c
            3187-3308           JAXUCZ010000016.1  83212608-83212729   c
            3309-3421           JAXUCZ010000016.1  83211874-83211986   c
            3422-5346           JAXUCZ010000016.1  83209280-83211204   c
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16q12.5"
     gene            1..5346
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /note="MCF.2 cell line derived transforming sequence-like"
                     /db_xref="GeneID:117020"
                     /db_xref="RGD:619782"
     exon            1..144
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     CDS             66..3593
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /note="isoform 2 is encoded by transcript variant 2;
                     guanine nucleotide exchange factor DBS; OST oncogene;
                     DBL's big sister; MCF2-transforming sequence-like protein;
                     mcf.2 transforming sequence-like"
                     /codon_start=1
                     /product="guanine nucleotide exchange factor DBS isoform
                     2"
                     /protein_id="NP_001400698.1"
                     /db_xref="GeneID:117020"
                     /db_xref="RGD:619782"
                     /translation="MRFWLRNEEMALEEMVQRLNAVSKNTDEIMHQDIVPLCAADIQE
                     QLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFI
                     LVIDRRQDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDEFKM
                     KVPVMMLSSVPELHGYIDKSQLTEDLGGTLDYCHSRWLCHRTAIESFALMVKQTAQML
                     QAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLALTEGNSILESLREPLAES
                     IVHSVNQDQLDNQATVKRLLTQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVK
                     TALDSMSQKIAAFTDVGNSLAHVQHLLKDLTTFEEKSSVAVDKARALSLEGQQLIENR
                     HYAVDSIHPKCEELQHLCDHFASEVTRRRDLLSKSLELHSLLETSMKWSDEGIFLLAS
                     QPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYKEYECILNQDLLEHVQKVFQ
                     KQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALTKSPSPSPGSWRSSENSSSEG
                     NALRRGPYRRAKSEMSEPRQGRTSSTGEEEESLAILRRHVMNELLDTERAYVEELLCV
                     LEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFLRELESCIDCPELVG
                     RCFLERMEEFQIYEKYCQNKPRSESLWRQCSDCPFFQECQKKLDHKLSLDSYLLKPVQ
                     RITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDL
                     GKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKA
                     PSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEVYIIQAPTPEIKAAWVNEIRKV
                     LTSQLQACREASQHRALEQSHSLPLPTPASTSPTKGSTRNVKKLEDRKTDPLCLEGCV
                     SSSLPKPPEKGKDDAVPSSTSESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEV
                     KSDPTPFGLRGWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPRKLVPGKYTV
                     LMDGEKGGSDTLAMRSGDMVEVVEEGTEGLWYVRDLTSSKEGWVPASSLATLLGKSSS
                     AQCLSSSGKTHCARQLCPEPAKILSPEPV"
     exon            145..228
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            229..343
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            344..434
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            435..554
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            555..671
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            672..821
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            822..946
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1061
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1181
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            1182..1373
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1565
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1725
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1799
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1876
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            1877..1943
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            1944..2059
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..2129
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            2130..2222
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            2223..2315
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            2316..2441
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            2442..2663
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            2664..2756
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            2757..2835
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            2836..2902
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            2903..3018
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            3019..3111
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            3112..3186
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            3187..3308
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            3309..3421
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
     exon            3422..5346
                     /gene="Mcf2l"
                     /gene_synonym="mcf 2l; Ost"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acccgacagc acctctggtg cagcggagga cgatcttctg gggatcggtt ttgttgagtc
       61 ggagaatgag gttttggctg agaaatgaag agatggccct agaagaaatg gtgcagagat
      121 taaacgcggt ttccaagaac actgatgaaa tcatgcacca ggacatcgtc ccactctgtg
      181 ccgctgacat ccaggagcag ctgaagaagc gctttgctta cctgtctggt gggcggggac
      241 aggacggaag cccagtcatc accttcccag actacccagc cttcagcgag atcccggaca
      301 aggagttcca gaatgtcatg acctatctca ccagcatccc cagtttgcaa gatgctggca
      361 tcgggttcat cttggtcata gaccgaagac aggacaaatg gacttctgtg aaggcgtcag
      421 tcctgcgaat agccgcatca ttcccagcca acctgcagct cgtcctcgtt ctccgaccca
      481 cgggcttctt ccagaggact ctctcggaca tagccttcaa gttcaacaga gatgagttta
      541 agatgaaagt accggtcatg atgctaagct cagtgccaga actacatggc tacattgaca
      601 agtcacagct gaccgaggac cttgggggga ccctggacta ctgccactcc aggtggctgt
      661 gccaccgcac agcgattgaa agcttcgccc tgatggtgaa gcagacagcc cagatgctgc
      721 aggcctttgg gaccgagctg gccgagactg agctgcccaa cgacgtccag tcaaccagcc
      781 tggtgctcag cgcacacaca gagaagaagg ctaaagtgaa ggaggatctg cagctggcgc
      841 tgacggaggg gaacagcatc cttgagagcc tcagggagcc gctggctgag agcatagtcc
      901 acagtgtgaa ccaggaccag ctggacaatc aggccactgt gaagaggctc ctgacccagc
      961 tgaatgagac cgaggctgcc tttgacgagt tctgggcaaa gcatcagcag aagctggaac
     1021 agtgcctaca gctgcgacat tttgaacaag gtttccggga ggtcaaaacc gccttggatt
     1081 ccatgtccca gaagatagcc gcgttcacag atgttggcaa cagcctggcc catgtgcagc
     1141 acctcctgaa ggacttgacc acctttgagg agaagtccag cgtggccgtg gacaaggcca
     1201 gagccctgtc cctggagggg cagcagctga ttgagaaccg gcactatgct gtagactcta
     1261 tccatccgaa gtgtgaggaa ctccagcacc tctgtgacca cttcgcctct gaggtcacca
     1321 ggaggcggga ccttctcagc aagtccttgg agctgcatag cctcctggag acgtccatga
     1381 agtggagcga cgagggcatc ttcctgctgg cctctcagcc tgtggacaaa tgccaatctc
     1441 aggatggtgc ggaggcagcc ctgcaggaga tcgagaagtt tctggagacc ggtgcagaaa
     1501 acaagattca ggagctcaat aagatttaca aggaatatga atgcatcctc aaccaggacc
     1561 ttctggaaca cgtgcaaaaa gtctttcaga agcaagagag caccgaggag atgttccacc
     1621 gcaggcaggc tagcctgaag aaactggcag ccaagcagac acggcctgtg cagcccgtgg
     1681 ccccccgacc agaggcgcta accaagtccc cctccccctc gccaggctcc tggcgaagct
     1741 ctgagaactc cagctctgag ggcaacgccc tccgcagagg gccctacagg agagccaaga
     1801 gcgaaatgag tgagccccgg cagggccgca ccagctccac aggagaagag gaggagagcc
     1861 tggccatcct gcgcagacac gtgatgaatg aacttctgga cacggagcgg gcctacgtgg
     1921 aggagctgct ctgtgtctta gagggctatg ctgcagagat ggacaacccc ttgatggcac
     1981 acctcatctc aacaggcctg caaaacaaga agaacattct gtttggaaac atggaggaaa
     2041 tctatcactt ccacaacaga atattcctgc gggagctgga gagctgcatc gactgcccag
     2101 agctggtggg gagatgcttt ctggagagga tggaggagtt ccagatctat gagaagtact
     2161 gtcagaacaa gccacgctcc gagagcctgt ggagacagtg ctctgactgt cccttcttcc
     2221 aggagtgcca gaagaagctg gaccacaagc tgagcctcga ctcctacctg ctgaagcctg
     2281 tgcagaggat aaccaagtac cagctgctgc tcaaggaaat gctgaagtac agcaagcact
     2341 gtgaaggggc cgaggacctg caagaggcac tgagctccat cctgggcatc ctcaaggcag
     2401 tgaacgactc catgcacctc atagccatca ctggctatga tggaaacctt ggggacctgg
     2461 ggaagctgct gatgcaaggc tccttcagcg tgtggacgga ccacaagaag ggccacacca
     2521 aggtgaagga gctagccagg ttcaagccca tgcagcggca cctcttcctt cacgagaagg
     2581 ccgtcctctt ctgcaagaag agggaggaga atggggaggg gtacgagaag gctccttcct
     2641 acagctacaa gcagtccttg aatatgactg ctgttggcat cacagagaat gtgaagggtg
     2701 acaccaagaa gttcgagatc tggtacaacg caagggagga ggtttacatc attcaggcac
     2761 caactcccga gattaaagca gcgtgggtga atgagattcg gaaggtgctg accagccaac
     2821 tgcaggcctg ccgggaagcc agccagcacc gagccctgga gcagtcccac agccttccct
     2881 tgcccacgcc agccagcacc agtcccacaa aagggagcac aagaaatgtc aagaagctcg
     2941 aagatcggaa gacggacccg ctctgcctgg aaggctgcgt gagctcatca ttaccaaagc
     3001 cccccgagaa gggcaaagac gatgcggtcc ctagttctac ctctgaaagc tctgcgcttt
     3061 ccagaaagcg cttcacgctg cagggcttgg ctaacctcaa aggtcagaaa gcctctccga
     3121 ccagtcctga caaaaaagct aagcggcatg aagtaaagag cgacccgact ccctttggtt
     3181 tgcgaggctg gagcaaaaca tcccactcac tggaggcccc tgaggaggac ggaggctggt
     3241 ccagtgctga ggagctcatc aattcatccg atgctgagga agacggagga gtgggcccta
     3301 ggaagctggt tccaggtaaa tacacagtgc tgatggatgg tgagaaaggg ggctctgaca
     3361 cccttgccat gaggagtgga gatatggtgg aggttgtgga agaaggaact gagggcctct
     3421 ggtatgttcg ggacctgacc agcagcaagg agggatgggt gccagccagc agcctagcca
     3481 ccctcctggg caaatccagc tcagcccagt gtctgagcag ctcaggtaag acccactgtg
     3541 cgcgccagct gtgccctgag cctgccaaga tcctcagccc agagcctgtt taaaagcaga
     3601 ggggtttcta ggtatgcaga gaaaagggcc agagccgaag cctgtctcca ctggtttccc
     3661 gcagactcca gcccagggtc agccgtgctg agcaactcat ccagctgcag cgagggctac
     3721 cctactccct tctccgagct ccaggggtag tggccaggcc tctgtggcag gcgctcaagg
     3781 ccaccgtgca gtagaccagc atgcaaatct ctaagttttt tctttttgct tgggcttgct
     3841 tagtttggga ggggatggtg ctccaagata tctggaaaca ggaggaccta tcaaccatgg
     3901 gcttcagagg acactggggc cttggaacca ggactctgct gcatatgcag aaggcaggag
     3961 acaacagttc accaccaaag cttcttcaga gactccaggg tggacagagg cagggctatg
     4021 ggtcaggaga ccccttgcag aaagatggga gacggccctt ttccacacat gcacagccag
     4081 cagcccctgc tatacagcct ctccacggtg tcctggcctc tctggtgctt tctacgggaa
     4141 catcatcctg aggaagccac ttctaaccgg agtaaagagt ttgctaccaa cagcctaaga
     4201 aggggatcaa gggactcttg tcttttataa gtttttgact ttttttttaa aaaaaacaaa
     4261 caaacaggga ttaatcccat ggccattttt ctgtggtact ctggcctcaa atcctaaacc
     4321 aagaatcact gtgtttacat gaaatgacaa tttgatactg tatttgatat aaaactattt
     4381 tttgttactg gggtttacat caaagcacgt tgtttataca actgactgat ttctggggca
     4441 ctctcactga gacctggcag cacaggtgct ggaaggcctg gtaagccagg gtcctgtcct
     4501 cctggtaagc ccagggcgtg tcctcttagc cagggggcgt gtcctcctgg ttagccaggg
     4561 aggggcgtgt cctcctggta agacaggggg cgtgtcctct tagccagggg gtgtgtcctc
     4621 ctggttagcc agggaggggc gtgtcctcca ctaaaagcgt tggaaatcac tgagagtctt
     4681 gcatctgcat aggtttcatt ttcacaagaa atgtaaagta taatttaaaa tattagcctt
     4741 cttttctaca atataaagga gactctctgt ttttttaaaa gaacatcatt aaattaggat
     4801 atttttatta tttttaaaga aaatgtaaaa ctccatgctg tcagccatcc tcagcaactc
     4861 aggcacaggc tgcctctcca tgtcagggca ggcccgactt cccctccccg agtcccaccc
     4921 acctctgccc tacagcagga ggggctgcaa ctgagctcat gggggggggg ggcctgcagc
     4981 ctccattcaa gtcaggcaga actgccacag actcttgttt ccccctttta tgtgtgtgtt
     5041 tcagaaatcg attctgttct ttggaaactc tgtgccgagt agggcaggta tttttatact
     5101 tgtgtatata tgttcacgtg tgtacatatg atctggtgta aaggatgtat ttatggtctc
     5161 acgctgctga acatgtgtca ctttacggtc cctccctgtc tcctcacccc gctgccatgg
     5221 gaggccaccg aggctcgttc accactgtat gtccaggaaa accaccctct tccctgccct
     5281 atggcttgca aagccattac tggctacacc ttcagagaat ggtaattaaa taaaaacgtg
     5341 caaaga
//
