LOCUS       XM_008773576            4650 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus MCF.2 cell line derived transforming
            sequence (Mcf2), mRNA.
ACCESSION   XM_008773576
VERSION     XM_008773576.3
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051356.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Jan 22, 2024 this sequence was replaced by
            NM_001427408.1.
            
            On Jan 21, 2021 this sequence version replaced XM_008773576.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..4650
                     /gene="Mcf2"
                     /note="MCF.2 cell line derived transforming sequence;
                     Derived by automated computational analysis using gene
                     prediction method: Gnomon. Supporting evidence includes
                     similarity to: 2 ESTs, 23 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 3 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:317598"
                     /db_xref="RGD:1566098"
     CDS             581..3913
                     /gene="Mcf2"
                     /codon_start=1
                     /product="proto-oncogene DBL"
                     /protein_id="XP_008771798.1"
                     /db_xref="GeneID:317598"
                     /db_xref="RGD:1566098"
                     /translation="MAEARSPPTGPSPAGPPPAGSPPAPALASPWLAFRNLPKAASRA
                     PRTRKRQLNPDNGTFVPFTQTIPVQVDMAMALHRVRIAEKYGKNYLCLLQAVFLAETG
                     LLLQLKDINHFLMQDIAFLSGGRGRDNAWIITFPENCNFRCIPEEVIAKVLNYLTSIA
                     RQSGSDSRFTIILDRRMDTWSSLKISLQKISASFPGNLHLVLVLRPTSFLQRTFTDIG
                     FRFSQEDFMLKLPVVMLSSVSDLLTYIDDKQLTPELGGTLQYCHSEWIIFRNAIEKFA
                     VTVKEMAQMLQSFGTELAETELPDDIPSIEEILAGRAERYHLLKNDLTTVTKEGKVLL
                     MNLQVPDTEENLSSSHECPQHINGDWQTIKKLLAQVHDMETAFDGFWEKHQLKMEQYL
                     QLWKFEQEFQEVVTQVEFLLSQQRELGDVTGNLAQVKQRLKKIEILDDKSQELLAKGR
                     IVILHGHKLASNHHYALDLICQRCNELRYLSDILVNEIRSKRIQLSRTFKMHRLLQQA
                     RQCCDQGECLLANRGMDKFQTKEDAQKALQDVDNFLQMAMPFINYDIENLQFEFDVLL
                     SPELKAQMKNIQRKLENIRSVFENQQNAYKISKDRPEGAVQRMIPAPESVMRARTIFF
                     SPKHVGIGYSFFQACKIFSKVKKSWRQIQAQSNVKVQATQDSQEKNFDQPPKLDSSLD
                     ILKNHVLNELIQTERAYVRELFTVLLGYRSEMDNPQMFDLMPPLLRNKKDVLFGNMAE
                     IYEFHNNIFMSRLEDCSDAPERVGPCFLERKEDFQMYAKYCQNKPRSELIWRKYSECA
                     FFQECQRKLKHRLGLDSYLLKPVQRITKYQLLLKELLKYSKEGEGTTKLKEALDSMLE
                     LLKSVNDSMHQTAINGYVGNMNDLGKMILQGAFSVWVGHRKGATKMKDFARFKPMQRH
                     LFLYEKAVMFCKRRFESGEGADRYPSYSFKHCLKMEEAGITEHVKGDNRKFEIWYGEK
                     EEIYIVQAPNIDVKMLWLKEIRNILLKQQELLTAKTQQDQLSDQDQLPPQQQNAEKQE
                     ETLLNAVEAGAEVEQASAMMEVREAALLVQAEANCTTWNGMLPCTEGTAATAEHPCNS
                     LSPNSDNGEDRSQMRSMSEVQF"
ORIGIN      
        1 ctatagatcc agccctttcc gacttgttgc tttgctgcta ccagcaaagc actccagtgt
       61 tatctgcagg tgcccatact gtgtatcctt attccattcc cgccctgccc cgggaatcag
      121 gatgggaagg aaggaacctc tggctccagc tcccactgcc tagagtgcga ggtcatgctg
      181 tctcctccct acacgtgcgc cagccttcct tacccttctg tggacgtctc tgtgggggct
      241 gtcccctctg gttcctggtg tcccgcccct gttcatcccg ttggaaacta ggcggggaca
      301 tgggtgtaca tccacaggga gcagagttgc actcaactct cagtggctga ggtcagagcg
      361 ctctgcagcc agagtcctga aatctctgca agtttctggg tttcctgtcc tgggtcttgt
      421 tggtcccagc tagggggctc tagtcttctg agccacggag ctgtgtagac tgcgaccctt
      481 ctggcagcgt ctgtgcttgc acaggcgggc tgctagcgag caagagtcca gcagtggcca
      541 gttggggttg gggtcaccaa cccgcaagtc tggcttgagg atggcggaag caaggtcccc
      601 tccaacaggg ccctctccag cagggccccc tccagcagga tcccctccgg ctcccgccct
      661 tgcctcgccg tggctggcgt tcaggaactt gcctaaggct gcctcccggg cgcccaggac
      721 taggaaacgg cagctaaatc cagacaacgg tactttcgtg cctttcacgc agacgatccc
      781 ggtgcaggtg gacatggcca tggccctgca tcgcgttagg atcgcagaga agtatgggaa
      841 gaactacctc tgcttgctgc aggcagtctt cctcgcagaa actgggctgc tcctccagtt
      901 aaaggatatc aatcatttcc taatgcaaga cattgccttc ctgtctggtg gccggggaag
      961 ggacaatgct tggatcatta cgtttccaga aaactgtaac ttcagatgca taccagagga
     1021 agtcattgca aaagtgctca attacttgac atctattgca agacaaagtg gatctgactc
     1081 ccgatttact attattctgg atcgaagaat ggatacatgg tcttccctca aaatttccct
     1141 ccagaaaatc tcagcctctt tccctgggaa cttacactta gtcctggttt tacgtcctac
     1201 cagttttctt caacggactt tcacagacat tggatttcga tttagccagg aggattttat
     1261 gctcaaatta ccagttgtta tgctgagctc agttagtgat ttgctgacat acattgatga
     1321 caagcagtta acccctgagt taggcggcac cttgcagtac tgccacagtg aatggatcat
     1381 cttcagaaat gccatagaga aatttgctgt cacagtgaaa gaaatggctc agatgttgca
     1441 atcctttgga acagaactgg ctgaaacaga gctaccagat gatatccctt caatagaaga
     1501 gattctggca ggtcgtgctg aaaggtatca tctattgaag aatgacttaa ctactgtaac
     1561 caaagaagga aaagttcttc tcatgaacct gcaagtgcct gacactgaag aaaatctcag
     1621 ttcaagtcat gaatgtcccc agcatataaa tggtgactgg caaactatta aaaagttgct
     1681 ggctcaggtg catgatatgg aaactgcttt tgatggattt tgggaaaaac atcagctgaa
     1741 aatggagcaa tatctgcaac tgtggaagtt tgagcaagaa tttcaagagg ttgtgactca
     1801 agttgaattt ttattaagtc aacagagaga actgggtgat gtaacgggga acttagctca
     1861 agtaaaacaa agacttaaaa aaatagagat cttggatgac aaatctcagg aactgttagc
     1921 caagggccgg attgtgatat tgcatggcca caagctggca tcgaatcacc actatgccct
     1981 tgatttaatc tgccagaggt gtaatgagct acgctacctt tctgatattt tagttaatga
     2041 gatcagatca aagcggatcc agctgagtag gactttcaaa atgcacagac ttctacagca
     2101 ggcacgtcag tgctgtgatc aaggggaatg cctcctggct aaccggggaa tggataagtt
     2161 tcagactaaa gaagatgctc agaaagccct tcaggatgtg gacaatttcc ttcaaatggc
     2221 catgcctttc atcaattatg atatcgaaaa cctgcaattt gaatttgatg tgctcctatc
     2281 tcctgaactc aaggctcaaa tgaagaatat acaacgaaag cttgaaaata ttcgaagtgt
     2341 atttgagaat caacagaatg cttataagat ctcaaaagat agacctgagg gggcagtcca
     2401 gcgtatgatt ccggcacctg aaagtgtgat gagagccaga acaatattct tttcacctaa
     2461 acatgttgga attggatatt ctttctttca agcatgtaaa attttttcaa aagtgaaaaa
     2521 gtcttggaga cagattcaag ctcagagcaa tgtgaaagtc caagccactc aggatagcca
     2581 agagaagaat tttgatcagc ctcccaaatt ggacagtagc ttggatattt taaagaacca
     2641 tgttctgaat gaactgatcc agacagagag ggcctatgtt cgagagctgt ttactgtttt
     2701 gttgggctac cgatctgaga tggacaatcc acagatgttt gatcttatgc cacctctcct
     2761 gagaaataaa aaggatgttc tctttggaaa tatggcagaa atatatgaat ttcataacaa
     2821 cattttcatg agcagactgg aagattgctc tgatgctcca gaaagagtgg gaccttgctt
     2881 cctggaaagg aaggaagatt ttcagatgta tgccaaatac tgtcagaata aacccaggtc
     2941 agagttaatt tggaggaagt attctgaatg tgcctttttc caggaatgtc aacggaaatt
     3001 gaaacatcga cttggtctgg attcctattt gctcaagcca gttcaacgaa tcactaagta
     3061 tcagttgctt ttaaaggagc ttttaaagta tagcaaagaa ggtgaaggaa ctactaaatt
     3121 gaaggaggcc cttgactcaa tgctggagct gctcaagtca gtgaatgatt ccatgcatca
     3181 gactgccatc aatggctatg ttggaaatat gaatgatctg ggaaagatga ttcttcaagg
     3241 tgccttcagt gtttgggtag gacatcggaa aggtgctacc aagatgaagg attttgcccg
     3301 gttcaaaccc atgcagagac accttttctt gtatgaaaaa gctgttatgt tttgtaagag
     3361 gcgtttcgaa tctggagaag gtgctgacag atacccatca tacagtttta agcactgcct
     3421 aaaaatggaa gaagctggta tcacagagca tgtgaaaggc gataaccgaa aatttgaaat
     3481 atggtatggt gagaaggaag aaatttatat tgttcaggct ccaaatattg atgtgaagat
     3541 gttatggcta aaagaaataa gaaacatttt gttgaagcag caggaacttt tgactgctaa
     3601 aacccagcag gatcagttaa gcgaccagga ccaacttcct ccccagcagc agaatgctga
     3661 aaagcaagaa gaaactcttt taaatgctgt ggaagcagga gcagaagtgg aacaagcgag
     3721 tgccatgatg gaggtcaggg aggcagctct gctagttcag gcagaagcaa attgcacaac
     3781 ttggaatggg atgctgccat gtacagaagg caccgctgcc actgctgagc atccatgcaa
     3841 ctctctctcc cctaacagcg acaatggaga agacaggtca caaatgagat ccatgtcgga
     3901 ggttcagttt taaagaagcc acagtgtcaa cctgtcactg ttttaaagaa gcaacagtgt
     3961 caagtcgttc tacctgcctg cttctccacc cacgtggaaa aaacaccact gaaaagtgtt
     4021 tcatcccaaa tggtgggatt tttccagatt aatatatgta aacatgcaca gaatcaacag
     4081 catagatgtc ttctttctct tcgtccactt caaagcattt tacttctagt actttttaat
     4141 ttgtaaaatt tcataagtgt gtaagacatt gtgcaggatc tcatctcaga atatttttga
     4201 cttgaaaatt gtttcttctc tgtctacttt acaaccaaat gcaatcagtg tgccttgaat
     4261 tgtttagctt actattaatt aagttaaact tagggctgta gaataattta ggccaagtga
     4321 agcacaacat tatgatttga agcgcttttt agacccagtg ctcctttaat ccatgcaaga
     4381 taatggcagc gttcagaagt cctggggggt ggggggcatt aataaattct attcagggct
     4441 aattgccttt tctatgtgcc aagatgaact tttcctgaaa gtagtttaca aattgcattc
     4501 aaaatgtaat attttcaagc ttgtttcttt ttccaaattt gggcttataa ttcatatagc
     4561 aaattagctg tgctctatta tcttctgtac atttcatgct gtaatccttt ttcccaaata
     4621 aaaatgaaac cttcaggttc tacaaattca
//
