LOCUS       NM_001012177            2220 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Rattus norvegicus methylcrotonyl-CoA carboxylase subunit 2 (Mccc2),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001012177 XM_342181
VERSION     NM_001012177.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Chu,C.H. and Cheng,D.
  TITLE     Expression, purification, characterization of human
            3-methylcrotonyl-CoA carboxylase (MCCC)
  JOURNAL   Protein Expr Purif 53 (2), 421-427 (2007)
   PUBMED   17360195
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Stadler,S.C., Polanetz,R., Meier,S., Mayerhofer,P.U.,
            Herrmann,J.M., Anslinger,K., Roscher,A.A., Roschinger,W. and
            Holzinger,A.
  TITLE     Mitochondrial targeting signals and mature peptides of
            3-methylcrotonyl-CoA carboxylase
  JOURNAL   Biochem Biophys Res Commun 334 (3), 939-946 (2005)
   PUBMED   16023992
REFERENCE   3  (bases 1 to 2220)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   4  (bases 1 to 2220)
  AUTHORS   Gallardo,M.E., Desviat,L.R., Rodriguez,J.M., Esparza-Gordillo,J.,
            Perez-Cerda,C., Perez,B., Rodriguez-Pombo,P., Criado,O., Sanz,R.,
            Morton,D.H., Gibson,K.M., Le,T.P., Ribes,A., de Cordoba,S.R.,
            Ugarte,M. and Penalva,M.A.
  TITLE     The molecular basis of 3-methylcrotonylglycinuria, a disorder of
            leucine catabolism
  JOURNAL   Am J Hum Genet 68 (2), 334-346 (2001)
   PUBMED   11170888
REFERENCE   5  (bases 1 to 2220)
  AUTHORS   Oei,J. and Robinson,B.H.
  TITLE     Simultaneous preparation of the three biotin-containing
            mitochondrial carboxylases from rat liver
  JOURNAL   Biochim Biophys Acta 840 (1), 1-5 (1985)
   PUBMED   3995077
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC083581.1.
            
            On Feb 11, 2005 this sequence version replaced XM_342181.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC083581.1, SRR8487231.54669.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q12"
     gene            1..2220
                     /gene="Mccc2"
                     /note="methylcrotonyl-CoA carboxylase subunit 2"
                     /db_xref="GeneID:361884"
                     /db_xref="RGD:1310279"
     exon            1..267
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..1830
                     /gene="Mccc2"
                     /EC_number="6.4.1.4"
                     /note="MCCase subunit beta; 3-methylcrotonyl-CoA
                     carboxylase 2; 3-methylcrotonyl-CoA:carbon dioxide ligase
                     subunit beta; 3-methylcrotonyl-CoA carboxylase
                     non-biotin-containing subunit; methylcrotonoyl-Coenzyme A
                     carboxylase 2 (beta); methylcrotonoyl-CoA carboxylase 2
                     (beta)"
                     /codon_start=1
                     /product="methylcrotonoyl-CoA carboxylase beta chain,
                     mitochondrial"
                     /protein_id="NP_001012177.1"
                     /db_xref="GeneID:361884"
                     /db_xref="RGD:1310279"
                     /translation="MWGALRSVLRPCSRASVPRQRAYHGDAVARLGTQPDSGSSTYQE
                     NYEQMKALVNQLHERAQYVRLGGSEKARARHTSRGKLLPRDRIDNLIDPGSPFLEFSQ
                     FAGYKLYGEEEVPAGGIITGIGRVSGVECMIVANDATVKGGTYYPVTVKKHVRAQEIA
                     LQNRLPCIYLVDSGGANLPRQADTFPDRDHFGRIFYNQAIMSSKNITQIAVVMGSCTA
                     GGAYVPAMADENIIVQRQGTIFLAGPPLVKAATGEEVSAEDLGGADLHCRRSGVTDHY
                     ALDDHHALHLTRKVVRSLNYQKKLDVTVEPSEEPLFPADELYGIVGANLKRSFDVREV
                     IARIVDGSRFNEFKALYGDTLVTGFARIFGYPVGIIGNNGVLFSESAKKGAHFVQLCC
                     QRNIPLLFLQNITGFMVGKDYEAEGIAKDGAKMVAAVSCAKVPKITVIIGGSYGAGNY
                     GMCGRAYSPRFLYMWPNARISVMGGEQAATVLATVARDQRAREGKQFSSAEEAALKEP
                     IIKRFEEEGNPYYSSARLWDDGIIDPVDTRLVLGLSISAALNAPIQRTDFGIFRM"
     exon            268..334
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     exon            335..419
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     exon            420..521
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     exon            522..649
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     exon            650..762
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     exon            763..876
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     exon            877..941
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1041
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1137
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1210
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1287
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1354
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1511
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1512..1626
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1712
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1713..2143
                     /gene="Mccc2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctggcctggg agcttggtgg accaaggctg gtggtgcaag aattctggag gaacagcggc
       61 ccggagctgc aaaactcagc tgcagggccg ctctctctct ttctctctct ctctttctct
      121 tgggtgcttg ctatcgccat gtggggcgcg ctgaggtcag ttctgcggcc ctgcagtcgg
      181 gcctccgtac cccggcagcg cgcctatcac ggggacgcgg tggcccgact gggcacccag
      241 cctgactcgg gctcctccac ctaccaggag aactatgagc agatgaaagc tttagtgaat
      301 caacttcatg aacgagccca gtatgtaaga ctgggaggga gtgagaaagc ccgggcgcga
      361 cacacatcaa gaggaaaact gttacccaga gaccgaatcg acaacctcat agacccaggg
      421 tctccctttc tggaattctc ccagttcgca ggttacaagt tatatggcga ggaggaagtc
      481 cctgcaggtg gcatcatcac aggcattggg cgagtgtctg gagtagaatg catgattgtg
      541 gctaacgatg ccaccgtcaa aggaggcacc tactacccag tgacggtgaa aaagcacgtt
      601 cgggcacaag agatcgcact gcagaacagg ctcccatgca tctatctggt tgactcagga
      661 ggggccaact tacctcggca agcagacacc tttccagacc gggaccactt tggccgcata
      721 ttctataatc aagctattat gtcttctaaa aatatcacac agatagctgt ggtcatgggc
      781 tcctgtacgg caggaggtgc atatgtgccg gccatggctg atgaaaacat cattgtacaa
      841 agacagggta ctatcttctt ggctggacca cccttggtga aggcagctac cggcgaagag
      901 gtgtccgctg aggatctcgg aggtgctgac ttgcactgca gaaggtctgg ggtcactgac
      961 cactatgctt tggatgacca ccacgccctt cacttgacaa ggaaggttgt gaggagtctc
     1021 aattatcaga agaaactgga tgttaccgtt gaaccttctg aagagccctt gttccccgca
     1081 gatgaattgt atgggatagt cggtgcgaac cttaagagga gctttgatgt ccgagaggtc
     1141 attgctagaa tcgtcgatgg aagcagattc aatgagttca aggccttgta tggagacacg
     1201 ctcgtcacag gattcgctcg aatatttggg tacccagtag gcatcattgg aaacaatgga
     1261 gttttgtttt ctgaatctgc caaaaagggc gctcacttcg tccagctgtg ctgccaaaga
     1321 aatatccccc tgctgtttct ccagaacatc actggattca tggttggtaa agactatgaa
     1381 gccgaaggga ttgccaagga tggcgccaag atggtagccg cagtatcctg tgccaaagtg
     1441 cctaagataa ctgtcataat cgggggatca tatggggctg gaaactacgg aatgtgtggc
     1501 agagcatata gtccaagatt tctctacatg tggccaaatg ctcgcatctc agtgatggga
     1561 ggagagcagg cagccaccgt gttagccacg gtggcgagag accagagagc ccgtgagggg
     1621 aagcagttct ccagtgcaga ggaggcagct ttgaaagagc ccatcatcaa gaggttcgaa
     1681 gaggaaggga acccttacta ttccagtgca aggctgtggg atgatgggat tatcgacccc
     1741 gtggacacca ggctggttct gggactcagc atcagtgcag ccctcaatgc gcccatccag
     1801 aggacagact ttggcatctt caggatgtaa cccagatacg gaacgtgttg ccacagctgt
     1861 aataaaaatt aacacgtgtg actagcctta aaacttgaga tccactgggc atttgctggc
     1921 acaggaattc ttttcttaac agtatgatgt atgggttttt ttttttttaa ttgtaaaaat
     1981 cagtgagtat tctgcttgat gagctttgat ttgtcaaaaa tttttcttac agacacttct
     2041 catggctcat tttttaccac ccataaaatg aagtgaccga ttttgcaatt atccttttga
     2101 gaaccacaaa cagtttgaaa aataaatctc tctaatctat gcaaaaaaaa aaaaaaaaaa
     2161 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//
