LOCUS       NP_076473                648 aa            linear   ROD 01-JUN-2024
DEFINITION  cell surface glycoprotein MUC18 isoform 1 precursor [Rattus
            norvegicus].
ACCESSION   NP_076473 XP_346824
VERSION     NP_076473.2
DBSOURCE    REFSEQ: accession NM_023983.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 648)
  AUTHORS   Yamchi,N.N., Rahbarghazi,R., Bedate,A.M., Mahdipour,M., Nouri,M.
            and Khanbabaee,R.
  TITLE     Menstrual blood CD146+ mesenchymal stem cells reduced fibrosis rate
            in the rat model of premature ovarian failure
  JOURNAL   Cell Biochem Funct 39 (8), 998-1008 (2021)
   PUBMED   34477225
  REMARK    GeneRIF: Menstrual blood CD146(+) mesenchymal stem cells reduced
            fibrosis rate in the rat model of premature ovarian failure.
REFERENCE   2  (residues 1 to 648)
  AUTHORS   Marr,N., Meeson,R., Kelly,E.F., Fang,Y., Peffers,M.J.,
            Pitsillides,A.A., Dudhia,J. and Thorpe,C.T.
  TITLE     CD146 Delineates an Interfascicular Cell Sub-Population in Tendon
            That Is Recruited during Injury through Its Ligand Laminin-alpha4
  JOURNAL   Int J Mol Sci 22 (18), 9729 (2021)
   PUBMED   34575887
  REMARK    GeneRIF: CD146 Delineates an Interfascicular Cell Sub-Population in
            Tendon That Is Recruited during Injury through Its Ligand
            Laminin-alpha4.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 648)
  AUTHORS   Obara,M., Sato,S., Takahashi,K., Kondo,Y., Hirose,M., Nata,K. and
            Taira,E.
  TITLE     Expression of cell adhesion molecule, Gicerin/CD146 during the
            formation of heart and in the cardiac hypertrophy
  JOURNAL   Mol Cell Biochem 476 (5), 2021-2028 (2021)
   PUBMED   33515199
  REMARK    GeneRIF: Expression of cell adhesion molecule, Gicerin/CD146 during
            the formation of heart and in the cardiac hypertrophy.
REFERENCE   4  (residues 1 to 648)
  AUTHORS   Barallobre-Barreiro,J., Oklu,R., Lynch,M., Fava,M., Baig,F.,
            Yin,X., Barwari,T., Potier,D.N., Albadawi,H., Jahangiri,M.,
            Porter,K.E., Watkins,M.T., Misra,S., Stoughton,J. and Mayr,M.
  TITLE     Extracellular matrix remodelling in response to venous
            hypertension: proteomics of human varicose veins
  JOURNAL   Cardiovasc Res 110 (3), 419-430 (2016)
   PUBMED   27068509
REFERENCE   5  (residues 1 to 648)
  AUTHORS   Liu,G., Sun,X., Bian,J., Wu,R., Guan,X., Ouyang,B., Huang,Y.,
            Xiao,H., Luo,D., Atala,A., Zhang,Y. and Deng,C.
  TITLE     Correction of diabetic erectile dysfunction with adipose derived
            stem cells modified with the vascular endothelial growth factor
            gene in a rodent diabetic model
  JOURNAL   PLoS One 8 (8), e72790 (2013)
   PUBMED   24023647
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 648)
  AUTHORS   Taira,E., Kohama,K., Tsukamoto,Y., Okumura,S. and Miki,N.
  TITLE     Gicerin/CD146 is involved in neurite extension of NGF-treated PC12
            cells
  JOURNAL   J Cell Physiol 204 (2), 632-637 (2005)
   PUBMED   15880440
  REMARK    GeneRIF: Increase of gicerin expression by NGF promotes the
            gicerin-gicerin homophilic interaction resulting in the neurite
            extension.
REFERENCE   7  (residues 1 to 648)
  AUTHORS   Taira,E., Kohama,K., Tsukamoto,Y., Okumura,S. and Miki,N.
  TITLE     Characterization of Gicerin/MUC18/CD146 in the rat nervous system
  JOURNAL   J Cell Physiol 198 (3), 377-387 (2004)
   PUBMED   14755543
REFERENCE   8  (residues 1 to 648)
  AUTHORS   Okumura,S., Kohama,K., Kim,S., Iwao,H., Miki,N. and Taira,E.
  TITLE     Induction of gicerin/CD146 in the rat carotid artery after balloon
            injury
  JOURNAL   Biochem Biophys Res Commun 313 (4), 902-906 (2004)
   PUBMED   14706627
  REMARK    GeneRIF: expression of gicerin was increased in the rat carotid
            arteries after balloon injury. Gicerin might be involved in the
            arteriosclerotic neointima formation in the artery.
REFERENCE   9  (residues 1 to 648)
  AUTHORS   Tsukamoto,Y., Egawa,M., Hiroi,S., Furuya,M., Tsuchiya,S.,
            Sasaki,F., Miki,N. and Taira,E.
  TITLE     Gicerin, an Ig-superfamily cell adhesion molecule, promotes the
            invasive and metastatic activities of a mouse fibroblast cell line
  JOURNAL   J Cell Physiol 197 (1), 103-109 (2003)
   PUBMED   12942546
REFERENCE   10 (residues 1 to 648)
  AUTHORS   Li,B.S., Su,Q.N., Kiyama,H., Miki,N., Robinow,D.R. and Zhang,L.
  TITLE     Expression of gicerin, a novel cell adhesion molecule, is
            upregulated in the astrocytes after hypoglossal nerve injury in
            rats
  JOURNAL   Neurosci Lett 260 (3), 149-152 (1999)
   PUBMED   10076889
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC070916.1 and AB035506.1.
            
            On Oct 6, 2005 this sequence version replaced NP_076473.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB035506.1, SRR8487230.91818.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Rattus norvegicus"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q22"
     Protein         1..648
                     /product="cell surface glycoprotein MUC18 isoform 1
                     precursor"
                     /note="cell surface glycoprotein MUC18; gicerin;
                     melanoma-associated antigen MUC18; l-gicerin"
                     /calculated_mol_wt=68951
     sig_peptide     1..23
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2394
     mat_peptide     24..648
                     /product="Cell surface glycoprotein MUC18.
                     /id=PRO_0000045461"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9EPF2.2)"
                     /calculated_mol_wt=68951
     Region          43..120
                     /region_name="CD19_double_Ig"
                     /note="CD19 (Cluster of Differentiation 19), a unique
                     double immunoglobulin (Ig)-fold domain; cl49627"
                     /db_xref="CDD:483967"
     Region          43..50
                     /region_name="strand B"
                     /note="strand B [structural motif]"
                     /db_xref="CDD:467824"
     Site            order(45,47,49,81,102,104,106,111)
                     /site_type="other"
                     /note="CD81 binding site [polypeptide binding]"
                     /db_xref="CDD:467824"
     Region          51..60
                     /region_name="CDR1"
                     /note="CDR1 [structural motif]"
                     /db_xref="CDD:467824"
     Site            58
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9EPF2.2)"
     Region          60..67
                     /region_name="strand C"
                     /note="strand C [structural motif]"
                     /db_xref="CDD:467824"
     Site            order(67,117)
                     /site_type="other"
                     /note="antibody binding site [polypeptide binding]"
                     /db_xref="CDD:467824"
     Region          76..80
                     /region_name="strand C'"
                     /note="strand C' [structural motif]"
                     /db_xref="CDD:467824"
     Region          81..90
                     /region_name="CDR2"
                     /note="CDR2 [structural motif]"
                     /db_xref="CDD:467824"
     Region          90..120
                     /region_name="protodomain 2+4"
                     /note="protodomain 2+4 [structural motif]"
                     /db_xref="CDD:467824"
     Region          90..94
                     /region_name="strand D"
                     /note="strand D [structural motif]"
                     /db_xref="CDD:467824"
     Region          101..106
                     /region_name="strand E"
                     /note="strand E [structural motif]"
                     /db_xref="CDD:467824"
     Region          112..120
                     /region_name="strand F"
                     /note="strand F [structural motif]"
                     /db_xref="CDD:467824"
     Region          141..236
                     /region_name="C2-set_2"
                     /note="CD80-like C2-set immunoglobulin domain; pfam08205"
                     /db_xref="CDD:400489"
     Region          173..177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          264..323
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          282..304
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9EPF2.2)"
     Region          348..412
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          <452..508
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          463..467
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          488..491
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          499..503
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Site            510
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9EPF2.2)"
     Region          527..554
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9EPF2.2)"
     Site            561..581
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9EPF2.2)"
     Site            608
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8R2Y2; propagated from
                     UniProtKB/Swiss-Prot (Q9EPF2.2)"
     Site            616
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P43121; propagated from
                     UniProtKB/Swiss-Prot (Q9EPF2.2)"
     Region          626..648
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9EPF2.2)"
     CDS             1..648
                     /gene="Mcam"
                     /gene_synonym="CD146; Muc18"
                     /coded_by="NM_023983.3:19..1965"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /db_xref="GeneID:78967"
                     /db_xref="RGD:620463"
ORIGIN      
        1 mglprlvcaf lfaaccccrs atgvpgeekq ptptpdpvev evgntallkc gpahpsgnfs
       61 qvewflihke rqipifrvhq gkgqsepgey ehrlslhgpg atlalsqvtp hddrmflcks
      121 kqprpqdhyv qlqvykapee ptiqanvlgi hvdiqelkev atcvgrngyp ipqviwykng
      181 rplqeeenrv hiqssqtves sglytlksvl sarlvkedkd aqfycelsyr lpsgnrmkes
      241 kevtvpvlyp aekvwvevep vgllkegdhv kircltdgnp qphftinkkn psteemeees
      301 tdengllsle paqkhhsgvy qcqsldlett vmlssdplel lvnyvsdvqv dptapevqeg
      361 dsltltckae snqdlefewl rdktgqllgk gpilqlnnvk reaggrylcv asvpsvpgln
      421 rtrrvsvgif gspwmaaker kvwaqenaml nlsceasghp qptiswning satewnpdpq
      481 tvvstlnvlv tpelletgae ctasnslgsn ttviilklvt lttltpdssq ttglstptvs
      541 phsranstst ekklpqqesk gvvivavivc tlvlavlgat lyyfykkgkl pcgrsgkqei
      601 tlpptrksef vvevksdklp eemallqgsn gdkrapgdqg ekyidlrh
//
