LOCUS       NM_172043               2095 bp    mRNA    linear   ROD 04-FEB-2024
DEFINITION  Rattus norvegicus MBL associated serine protease 2 (Masp2), mRNA.
ACCESSION   NM_172043 XM_342977
VERSION     NM_172043.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Nan,R., Furze,C.M., Wright,D.W., Gor,J., Wallis,R. and Perkins,S.J.
  TITLE     Flexibility in Mannan-Binding Lectin-Associated Serine Proteases-1
            and -2 Provides Insight on Lectin Pathway Activation
  JOURNAL   Structure 25 (2), 364-375 (2017)
   PUBMED   28111019
  REMARK    GeneRIF: Flexibility in MASP-1 and MASP-2 provides insight on
            lectin pathway activation.
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Kidmose,R.T., Laursen,N.S., Dobo,J., Kjaer,T.R., Sirotkina,S.,
            Yatime,L., Sottrup-Jensen,L., Thiel,S., Gal,P. and Andersen,G.R.
  TITLE     Structural basis for activation of the complement system by
            component C4 cleavage
  JOURNAL   Proc Natl Acad Sci U S A 109 (38), 15425-15430 (2012)
   PUBMED   22949645
REFERENCE   3  (bases 1 to 2095)
  AUTHORS   Venkatraman Girija,U., Furze,C., Toth,J., Schwaeble,W.J.,
            Mitchell,D.A., Keeble,A.H. and Wallis,R.
  TITLE     Engineering novel complement activity into a pulmonary surfactant
            protein
  JOURNAL   J Biol Chem 285 (14), 10546-10552 (2010)
   PUBMED   20118239
REFERENCE   4  (bases 1 to 2095)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   5  (bases 1 to 2095)
  AUTHORS   Girija,U.V., Dodds,A.W., Roscher,S., Reid,K.B. and Wallis,R.
  TITLE     Localization and characterization of the mannose-binding lectin
            (MBL)-associated-serine protease-2 binding site in rat ficolin-A:
            equivalent binding sites within the collagenous domains of MBLs and
            ficolins
  JOURNAL   J Immunol 179 (1), 455-462 (2007)
   PUBMED   17579066
  REMARK    GeneRIF: A lysine residue (Lys56) within the conserved motif is
            essential for MASP-2 binding and probably mediates the specificity
            of the ficolin-MASP interaction.
REFERENCE   6  (bases 1 to 2095)
  AUTHORS   Wallis,R. and Dodd,R.B.
  TITLE     Interaction of mannose-binding protein with associated serine
            proteases: effects of naturally occurring mutations
  JOURNAL   J Biol Chem 275 (40), 30962-30969 (2000)
   PUBMED   10913141
REFERENCE   7  (bases 1 to 2095)
  AUTHORS   Matsushita,M., Thiel,S., Jensenius,J.C., Terai,I. and Fujita,T.
  TITLE     Proteolytic activities of two types of mannose-binding
            lectin-associated serine protease
  JOURNAL   J Immunol 165 (5), 2637-2642 (2000)
   PUBMED   10946292
REFERENCE   8  (bases 1 to 2095)
  AUTHORS   Stover,C.M., Thiel,S., Lynch,N.J. and Schwaeble,W.J.
  TITLE     The rat and mouse homologues of MASP-2 and MAp19, components of the
            lectin activation pathway of complement
  JOURNAL   J Immunol 163 (12), 6848-6859 (1999)
   PUBMED   10586086
REFERENCE   9  (bases 1 to 2095)
  AUTHORS   Stover,C.M., Thiel,S., Thelen,M., Lynch,N.J., Vorup-Jensen,T.,
            Jensenius,J.C. and Schwaeble,W.J.
  TITLE     Two constituents of the initiation complex of the mannan-binding
            lectin activation pathway of complement are encoded by a single
            structural gene
  JOURNAL   J Immunol 162 (6), 3481-3490 (1999)
   PUBMED   10092804
REFERENCE   10 (bases 1 to 2095)
  AUTHORS   Thiel,S., Vorup-Jensen,T., Stover,C.M., Schwaeble,W., Laursen,S.B.,
            Poulsen,K., Willis,A.C., Eggleton,P., Hansen,S., Holmskov,U.,
            Reid,K.B. and Jensenius,J.C.
  TITLE     A second serine protease associated with mannan-binding lectin that
            activates complement
  JOURNAL   Nature 386 (6624), 506-510 (1997)
   PUBMED   9087411
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CO557085.1 and AJ277747.1.
            
            [WARNING] On May 1, 2024 this sequence was replaced by NM_172043.2.
            
            On Oct 24, 2005 this sequence version replaced XM_342977.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC128700.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760400
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                CO557085.1         1-58
            59-2095             AJ277747.1         1-2037
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..2095
                     /gene="Masp2"
                     /gene_synonym="MAp19; MASP-2"
                     /note="MBL associated serine protease 2"
                     /db_xref="GeneID:64459"
                     /db_xref="RGD:620214"
     exon            1..42
                     /gene="Masp2"
                     /gene_synonym="MAp19; MASP-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             38..2095
                     /gene="Masp2"
                     /gene_synonym="MAp19; MASP-2"
                     /EC_number="3.4.21.104"
                     /note="mannose binding lectin-associated serine
                     protease-2; mannose-binding protein-associated serine
                     protease 2; mannan-binding lectin serine protease 2;
                     mannan-binding lectin serine peptidase 2"
                     /codon_start=1
                     /product="mannan-binding lectin serine protease 2
                     precursor"
                     /protein_id="NP_742040.1"
                     /db_xref="GeneID:64459"
                     /db_xref="RGD:620214"
                     /translation="MRLLIVLGLLWSLVATLLGSKWPEPVFGRLVSPGFPEKYGNHQD
                     RSWTLTAPPGFRLRLYFTHFNLELSYRCEYDFVKLTSGTKVLATLCGQESTDTERAPG
                     NDTFYSLGPSLKVTFHSDYSNEKPFTGFEAFYAAEDVDECRTSLGDSVPCDHYCHNYL
                     GGYYCSCRVGYILHQNKHTCSALCSGQVFTGRSGFLSSPEYPQPYPKLSSCAYNIRLE
                     EGFSITLDFVESFDVEMHPEAQCPYDSLKIQTDKREYGPFCGKTLPPRIETDSNKVTI
                     TFTTDESGNHTGWKIHYTSTAQPCPDPTAPPNGHISPVQATYVLKDSFSVFCKTGFEL
                     LQGSVPLKSFTAVCQKDGSWDRPIPECSIIDCGPPDDLPNGHVDYITGPEVTTYKAVI
                     QYSCEETFYTMSSNGKYVCEADGFWTSSKGEKSLPVCKPVCGLSTHTSGGRIIGGQPA
                     KPGDFPWQVLLLGETTAAGALIHDDWVLTAAHAVYGKTEAMSSLDIRMGILKRLSPHY
                     TQAWPEAVFIHEGYTHGAGFDNDIALIKLKNKVTINRNIMPICLPRKEAASLMKTDFV
                     GTVAGWGLTQKGFLARNLMFVDIPIVDHQKCATAYTKQPYPGAKVTVNMLCAGLDAGG
                     KDSCRGDSGGALVFLDNETQRWFVGGIVSWGSINCGGSEQYGVYTKVTNYIPWIENII
                     NNF"
     exon            43..271
                     /gene="Masp2"
                     /gene_synonym="MAp19; MASP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            272..449
                     /gene="Masp2"
                     /gene_synonym="MAp19; MASP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            450..581
                     /gene="Masp2"
                     /gene_synonym="MAp19; MASP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            582..778
                     /gene="Masp2"
                     /gene_synonym="MAp19; MASP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            779..926
                     /gene="Masp2"
                     /gene_synonym="MAp19; MASP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1045
                     /gene="Masp2"
                     /gene_synonym="MAp19; MASP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1124
                     /gene="Masp2"
                     /gene_synonym="MAp19; MASP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1256
                     /gene="Masp2"
                     /gene_synonym="MAp19; MASP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1331
                     /gene="Masp2"
                     /gene_synonym="MAp19; MASP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..2095
                     /gene="Masp2"
                     /gene_synonym="MAp19; MASP-2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgacaggcgc tggacctgca gagctgggtg gcacaccatg aggctactga tcgtcctggg
       61 tctgctttgg agtttggtgg ccacactttt gggctccaag tggcctgagc ctgtattcgg
      121 gcgcctggtg tcccctggct tcccagagaa gtatggcaac catcaggatc gatcctggac
      181 gctgactgca ccccctggct tccgcctgcg cctctacttc acccacttca acctggaact
      241 ctcttaccgc tgcgagtatg actttgtcaa gttgacctca gggaccaagg tgctagccac
      301 gctgtgtggg caggagagta cagatactga gcgggcacct ggcaatgaca ccttctactc
      361 actgggtccc agcctaaagg tcaccttcca ctccgactac tccaatgaga agccattcac
      421 aggatttgag gccttctatg cagcggagga tgtggatgaa tgcagaacat ccctgggaga
      481 ctcagtccct tgtgaccatt attgccacaa ctacctgggc ggctactact gctcctgccg
      541 agtgggctac attctgcacc agaacaagca tacctgctca gccctttgtt caggccaggt
      601 gttcactggg aggtctggct ttctcagtag ccctgagtac ccacagccat accccaaact
      661 ctccagctgc gcctacaaca tccgcctgga ggaaggcttc agtatcaccc tggacttcgt
      721 ggagtccttt gatgtggaga tgcaccctga agcccagtgc ccctacgact ccctcaagat
      781 tcaaacagac aagagggaat acggcccgtt ttgtgggaag acgctgcccc ccaggattga
      841 aactgacagc aacaaggtga ccattacctt taccaccgac gagtcaggga accacacagg
      901 ctggaagata cactacacaa gcacagcaca gccctgccct gatccaacgg cgccacctaa
      961 tggtcacatt tcacctgtgc aagccacgta tgtcctgaag gacagctttt ctgtcttctg
     1021 caagactggc ttcgagcttc tgcaaggttc tgtccccctg aagtcattca ctgctgtctg
     1081 tcagaaagat ggatcttggg accggccgat accagagtgc agcattattg actgtggccc
     1141 tcccgatgac ctacccaatg gccacgtgga ctatatcaca ggccctgaag tgaccaccta
     1201 caaagctgtg attcagtaca gctgtgaaga gactttctac acaatgagca gcaatggtaa
     1261 atatgtgtgt gaggctgatg gattctggac gagctccaaa ggagaaaaat ccctcccggt
     1321 ttgcaagcct gtctgtggac tgtccacaca cacttcagga ggccgtataa ttggaggaca
     1381 gcctgcaaag cctggtgact ttccttggca agtcttgtta ctgggtgaaa ctacagcagc
     1441 aggtgctctt atacatgacg actgggtcct aacagcggct catgctgtat atgggaaaac
     1501 agaggcgatg tcctccctgg acatccgcat gggcatcctc aaaaggctct cccctcatta
     1561 cactcaagcc tggccagagg ctgtctttat ccatgaaggc tacactcacg gagctggttt
     1621 tgacaatgat atagcactga ttaaactcaa gaacaaagtc acaatcaaca gaaacatcat
     1681 gccgatttgt ctaccaagaa aagaagctgc atccttaatg aaaacagact tcgttggaac
     1741 tgtggctggc tgggggttaa cccagaaggg gtttcttgct agaaacctaa tgtttgtgga
     1801 cataccaatt gttgaccacc aaaaatgtgc tactgcgtat acaaagcagc cctacccagg
     1861 agcaaaagtg actgttaaca tgctctgtgc tggcctagac gccggtggca aggacagctg
     1921 cagaggtgac agcggagggg cattagtgtt tctagacaat gaaacacaga gatggtttgt
     1981 gggaggaata gtttcctggg gttctattaa ctgtgggggg tcagaacagt atggggtcta
     2041 cacgaaagtc acgaactata ttccctggat tgagaacata ataaataatt tctaa
//
