LOCUS       NM_001401674            5234 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus membrane associated ring-CH-type finger 8
            (Marchf8), transcript variant 3, mRNA.
ACCESSION   NM_001401674 XM_017592643
VERSION     NM_001401674.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Lapaque,N., Jahnke,M., Trowsdale,J. and Kelly,A.P.
  TITLE     The HLA-DRalpha chain is modified by polyubiquitination
  JOURNAL   J Biol Chem 284 (11), 7007-7016 (2009)
   PUBMED   19117940
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Ohmura-Hoshino,M., Matsuki,Y., Aoki,M., Goto,E., Mito,M.,
            Uematsu,M., Kakiuchi,T., Hotta,H. and Ishido,S.
  TITLE     Inhibition of MHC class II expression and immune responses by c-MIR
  JOURNAL   J Immunol 177 (1), 341-354 (2006)
   PUBMED   16785530
  REMARK    Erratum:[J Immunol. 2006 Sep 1;177(5):3493]
REFERENCE   3  (bases 1 to 5234)
  AUTHORS   Bartee,E., Mansouri,M., Hovey Nerenberg,B.T., Gouveia,K. and
            Fruh,K.
  TITLE     Downregulation of major histocompatibility complex class I by human
            ubiquitin ligases related to viral immune evasion proteins
  JOURNAL   J Virol 78 (3), 1109-1120 (2004)
   PUBMED   14722266
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On Feb 17, 2022 this sequence version replaced XM_017592643.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643298.24865.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-194               JAXUCZ010000004.1  151088506-151088699
            195-295             JAXUCZ010000004.1  151088877-151088977
            296-466             JAXUCZ010000004.1  151135431-151135601
            467-517             JAXUCZ010000004.1  151175336-151175386
            518-603             JAXUCZ010000004.1  151195119-151195204
            604-1449            JAXUCZ010000004.1  151195419-151196264
            1450-1630           JAXUCZ010000004.1  151197470-151197650
            1631-1778           JAXUCZ010000004.1  151199290-151199437
            1779-5234           JAXUCZ010000004.1  151199750-151203205
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q42"
     gene            1..5234
                     /gene="Marchf8"
                     /gene_synonym="March8; Mir"
                     /note="membrane associated ring-CH-type finger 8"
                     /db_xref="GeneID:312656"
                     /db_xref="RGD:1309339"
     exon            1..194
                     /gene="Marchf8"
                     /gene_synonym="March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            195..295
                     /gene="Marchf8"
                     /gene_synonym="March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            296..466
                     /gene="Marchf8"
                     /gene_synonym="March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     CDS             374..2080
                     /gene="Marchf8"
                     /gene_synonym="March8; Mir"
                     /EC_number="2.3.2.27"
                     /note="isoform 2 is encoded by transcript variant 3;
                     c-mir, cellular modulator of immune recognition; E3
                     ubiquitin-protein ligase MARCH8; membrane-associated ring
                     finger (C3HC4) 8, E3 ubiquitin protein ligase; E3
                     ubiquitin-protein ligase MARCHF8"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase MARCHF8 isoform 2"
                     /protein_id="NP_001388603.1"
                     /db_xref="GeneID:312656"
                     /db_xref="RGD:1309339"
                     /translation="MSMPLHQISAIPSQDAISARVYRSKTKDKEQNEKTLGHSMSHPS
                     NISKAGSSPPSTTAPVSAFSRTSVTPSNQDICSSSAVFSECCHYSPVQSAVVLKAPPC
                     QSSLTQGLTVTVICKDTLQASQKNPFGSDWDQVLRPAKNTKARRALRFSKSLSDVGEK
                     TQDTLESFDYMERTCSEGKLVLPQGPSLKKNRLHHKDKGAEHCPPLSHSKHSPILSLA
                     TNHSAVSKREAGKGSVRIPLLEEKADGEARLRSQRLLRYLFSLSRGSSASSLPRFHEL
                     ENHASRLHTAKSSSWLAGSMGFCSDEMGDDDVFEDASSAKLKNRVLRAPLCSVEKDSD
                     LDCPSVLSEKCTPISPVSTSGDACRICHCEGDDESPLITPCHCTGSLHFVHQACLQQW
                     IKSSDTRCCELCKYEFIMETKLKPLRKWEKLQMTASERRKIMCSVTFHVIAITCVVWS
                     LYVLIDRTAEEIKQGQVTGILEWPFWTKLVVVAIGFTGGLLFMYVQCKVYLQLWKRLK
                     AYNRVIYVQNCPETSKKNIFEKSALTEPTLENKEGHGMCHSTTNSSCTEPEDTGAEII
                     NV"
     exon            467..517
                     /gene="Marchf8"
                     /gene_synonym="March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            518..603
                     /gene="Marchf8"
                     /gene_synonym="March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            604..1449
                     /gene="Marchf8"
                     /gene_synonym="March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1630
                     /gene="Marchf8"
                     /gene_synonym="March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            1631..1778
                     /gene="Marchf8"
                     /gene_synonym="March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..5234
                     /gene="Marchf8"
                     /gene_synonym="March8; Mir"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtcggcgtc ctcccgctgg gtggtgtagg ctgcgtactg acccagccgc cctgctgcgt
       61 ccggcctgtg tcatgcagga agcgggccgc gcgtggagct gcgcgcccac ctgagctgct
      121 tgcgccgagg tgcggcgtgg cggggccgcg cgctcccggc cgccaggggt gtggccgctg
      181 ggaggccgcg acagtctcca ggtccgagat ggaagtgtgg attcgccttc tctgagtgcc
      241 tgacgtggcg tctggagggt gctgttcaag tagaagcgct ggagggaaag cgtagcttga
      301 agtcaatggc ccaaaatgac tctgctagtg gagactcact tccgtaagga ggaaaaccag
      361 gtcagaggtt gggatgagca tgccactgca ccagatctct gccatccctt ctcaggatgc
      421 catttctgct agagtctaca ggagcaagac caaagataag gagcagaatg agaagacttt
      481 gggacattcc atgagtcatc caagcaacat ttctaaggct gggagtagtc ctccatccac
      541 gaccgctcca gtgtctgcct tctctcgcac ttctgtaaca ccatccaacc aggacatctg
      601 cagttccagt gcagtgtttt ctgagtgttg tcactacagt cccgtgcagt ctgctgttgt
      661 cttgaaagct cctccatgcc agagttctct gacacaaggg ctcactgtga cagttatctg
      721 taaagacaca ttacaggcat cacagaaaaa tccctttggt tcagactggg accaagtctt
      781 gaggcctgct aaaaatacca aggccagaag agcactcaga ttctcaaagt cactaagtga
      841 tgtgggtgag aagacccagg atactttgga aagctttgac tatatggaaa gaacttgttc
      901 tgaagggaaa ttggtgcttc ctcaaggtcc atctctcaaa aagaacaggc tccatcataa
      961 agacaaagga gcagagcact gcccacccct cagccattcc aaacactctc ctatcttatc
     1021 ccttgctacc aaccattcag ctgtctcaaa gagggaagct ggcaagggaa gcgtgcgcat
     1081 cccacttttg gaggagaaag ctgatggtga ggccaggttg agaagccagc gactgctccg
     1141 gtacctgttc tcactctccc gtggctcaag tgccagcagc ctacctaggt tccatgagct
     1201 ggagaaccat gccagccgtc tgcacactgc caagtcgtcc agctggctgg cagggagcat
     1261 gggtttctgt tccgatgaaa tgggagacga cgacgtcttt gaggatgcat catctgccaa
     1321 attgaagaac agggtcctgc gtgcgcccct gtgctcagta gagaaggata gtgacctgga
     1381 ttgtccttct gtgctctctg aaaaatgcac tcctatctct cctgtgtcta cttcagggga
     1441 tgcctgcagg atctgccact gtgaaggcga tgacgagagc cctctgatca ccccctgtca
     1501 ctgtacaggg agcctacact ttgtgcatca ggcttgcctg cagcagtgga ttaagagttc
     1561 tgacacacgc tgctgtgaac tctgcaaata cgagttcatc atggagacca agctgaaacc
     1621 tttgaggaaa tgggagaagt tgcagatgac ggccagtgag cgcaggaaga tcatgtgctc
     1681 agtgacgttc catgtcattg ccatcacctg tgtggtctgg tccttgtatg tactcattga
     1741 ccgcacagca gaggagatca agcagggtca ggtgacagga atcctagagt ggcctttctg
     1801 gactaagctg gtagttgtgg ccatcggctt caccggagga cttctcttta tgtatgtcca
     1861 atgcaaggtg tacctacagt tatggaaaag actcaaggct tacaatagag tgatctatgt
     1921 tcaaaactgt ccagaaacaa gtaaaaagaa tatttttgaa aagtctgcac taacagagcc
     1981 cacccttgaa aataaagaag gacatggaat gtgtcattcc accacaaatt cttcttgcac
     2041 agagcctgaa gacactggag cagaaattat taacgtctga ccatgtggag ggtgccattt
     2101 ccttagtgtc catcaacagc tgaagggatt tgtttactgc caattgtgtt ccttccctcc
     2161 gtcgtttaat agcatagact gggcaggtga ctatttatag tggtctcttt ttctcagcct
     2221 tccttggtga gagtgtccta gaattcctgt ggagcaggta ccactgggcc ccattctgtc
     2281 aggcttctct gtggtctggg aaggtgggag accccatgta tccacaatga ggagctggct
     2341 gtgttccagc ccctggtctt gagtagatta tggaagtaaa aagctaaagt agaagacaga
     2401 gcagcatgag ggagatggat gatggaagat gcccagaggg cagggcagca tggtacggga
     2461 agctgctggt tgggagggtt ccctctgact aacaagagct agaggtggga agggaacagt
     2521 gcctacaaat aagcccctgc ctcgttcagc tgatgctgcc gccttgctct caggcagatg
     2581 aggccttggg gccccacatc tgctggtgtg cgattcactg agcaaacagc actttatgga
     2641 agttcttatt ttggaattcc tgtgtctaat gggagtgaca cttttaaaag cgtgtcacat
     2701 ttagaaatgt tctctttatt gcctgttatc tcttacatca aaattgaact gaaaccattt
     2761 ctgccttata tattctgagt agtagatgtc cttccatgtc aagaaaagac ttgagactta
     2821 aagcaagaca ggcaggtctg agactccatg ggaattaatg gatgccatgg gcgtaattcc
     2881 tctggttttg agtctgtttc ctgaaaagcc ctatctcacg agcccacccg tacatttcta
     2941 tgttgtgagc attgaaatgg aaggggtttg agatctaatg gatctgactc agcgtgagca
     3001 tttagaaaat gctcctttga ctgacagatg tctccagatg gagaggacac ccctgtgatc
     3061 cagctctgct gtctgctgtg ctgacaaagt tctgtggagt cactgctgaa agatacgagg
     3121 taaacaggcc agctctgggt tgaggccttg gcctgagcag tcagttaact gcatgagcca
     3181 ctgtgtcact gaccttgccc cacacccact tatcgcctta accagacttg acaggataat
     3241 ttagggtcct tagctccaca catgatctag tctgtctttg gggttacctc ctgcactttt
     3301 tttcacaaga cagacaaaac caaatcacta ttgctaaatg gcctttgccc ctttaaaaag
     3361 tctgttgaac attttttttt tcttgaaggc cattgtcaca gtgccagaca atagtcaact
     3421 cacaatgtga caaggggaac cacagaagtg tcctctccaa gtggctgctg tcggctacag
     3481 ggtcttcaga ttctcttgag gtggccaggg caatgcctag ggtctggtcc atctccccga
     3541 gctttggctg cttcacttgg cttcttcaga gaccaaggca aaccgaagat aaatctttct
     3601 gagttcctag tagaagctct gtgtttagtt ttcaggagca ggtagaggct agcaggcttg
     3661 tggaagcaag ttctgtcctg ttctcccctt gtctgctccc tggagtcatg gtttccttgt
     3721 gaagctcttc tgccagggct tccagccatt gtctaacggg cccaccctga ggtcagcctg
     3781 ctctctctcc cagaccacat aagcctcctg tgctcactgt gcctgcccca aagtgcaatt
     3841 actcacatgc agtgagggca ctgggtagct acagactgcc cactaggccc tgactattct
     3901 ttttagtaac tgctgcctct gagtctctgg tcctatccat ctgtctgtga catgatgctc
     3961 caacagtggg tgtacagtca aatgaagacg gaggcttttg tagagaagag agcttacctc
     4021 agtggcggtt gtctctgcac agctgaccag ggcttgtact cccaccatgg cattcatgta
     4081 gttcccttca tttcaggaag cctaatggta tgtcttcttt ggcttctctg atgaacgctg
     4141 tccttcagcc ttcttcctct cccagccacc ccttgcttct ccccccaccc cacctccagg
     4201 ttctctgatc tagaaaacaa ctacacatgg gcgactcagt gcaggagact ggcaaggcat
     4261 ggtatgaagg tctggcagag ctctgggctc ttccagtctt ctctagtcct gggcctctga
     4321 gtaatttgca gggctatctg tctaccccat tgctcctcac tttagcttag atgcagtggg
     4381 aagatggcat gtagttggac caagcagatc tgggatgatg ccccaggcca ggagggcttg
     4441 tgcttgtggg tcaagtgaag aagaagtagt ctcagggtgt cactggccgc cctctctaac
     4501 ttggccttta ccccgtatgt catagacccg ttcacacagc tctgacttta gctgccacaa
     4561 gtgagtagtt gggggttgtg ctcaagtgta tcacaagaat ttcctggaaa tgtaagcatt
     4621 ctcttcttag tctaggtttc tctgactctc ctttctggtt tcggtacact cttcagtcat
     4681 gtaggcttag tttctaaacc tgtgggtgtg tccaagtgga cacagatgtg caggctcagg
     4741 cagttgagga taggatagtt taaggccttg ggtcatgtct aagtgacagg agagtgaagg
     4801 ctgtgtgggc agggtgaaga tcttgtgggt gtggttagat gcacatgcat atttgaaata
     4861 ctccgtgttc aaatctggtt tgaaataatg cagctcaagg aaagcccaaa gcgtagggag
     4921 gtcaaaggca cttgtctgtg gggagtgagc gatgaaggcg cgtaactggt gcacatttag
     4981 atgcaatgtt tcaagttgat tgttaataaa aaccaaattt acactggtgt gtgtagtgta
     5041 gtttctaaaa agcacttcac attttaaatt tttcttggaa aatttctagt aatctaaatg
     5101 tctaattttt taatcttttt gtgtatgttc atgtttctca gtattactgc ttgaacgact
     5161 ctctgtacgg ggatttgtat gtgctataca cggggtgtct aaaggtagca ataaagcctt
     5221 tcgttacaaa ggga
//
