LOCUS       NM_001305274            6258 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus membrane associated ring-CH-type finger 6
            (Marchf6), mRNA.
ACCESSION   NM_001305274 XM_001065952 XM_215517
VERSION     NM_001305274.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Zhang,X., Jiang,Y., Han,W., Liu,A., Xie,X., Han,C., Fan,C.,
            Wang,H., Zhang,H., Ding,S., Shan,Z. and Teng,W.
  TITLE     Effect of Prolonged Iodine Overdose on Type 2 Iodothyronine
            Deiodinase Ubiquitination-Related Enzymes in the Rat Pituitary
  JOURNAL   Biol Trace Elem Res 174 (2), 377-386 (2016)
   PUBMED   27156111
  REMARK    GeneRIF: High iodine intake had no discernible effects on USP20.
            Temporary increases in USP33 and USP19 mRNA levels were observed.
            The enzymes related to D2 ubiquitination change with prolonged high
            iodine intake. Increased D2 ubiquitination suppresses the activity
            of D2, causing a decrease in negative feedback of the
            hypothalamic-pituitary-thyroid axis.
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Nakamura,N., Harada,K., Kato,M. and Hirose,S.
  TITLE     Ubiquitin-specific protease 19 regulates the stability of the E3
            ubiquitin ligase MARCH6
  JOURNAL   Exp Cell Res 328 (1), 207-216 (2014)
   PUBMED   25088257
REFERENCE   3  (bases 1 to 6258)
  AUTHORS   Zelcer,N., Sharpe,L.J., Loregger,A., Kristiana,I., Cook,E.C.,
            Phan,L., Stevenson,J. and Brown,A.J.
  TITLE     The E3 ubiquitin ligase MARCH6 degrades squalene monooxygenase and
            affects 3-hydroxy-3-methyl-glutaryl coenzyme A reductase and the
            cholesterol synthesis pathway
  JOURNAL   Mol Cell Biol 34 (7), 1262-1270 (2014)
   PUBMED   24449766
REFERENCE   4  (bases 1 to 6258)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   5  (bases 1 to 6258)
  AUTHORS   Zavacki,A.M., Arrojo E Drigo,R., Freitas,B.C., Chung,M.,
            Harney,J.W., Egri,P., Wittmann,G., Fekete,C., Gereben,B. and
            Bianco,A.C.
  TITLE     The E3 ubiquitin ligase TEB4 mediates degradation of type 2
            iodothyronine deiodinase
  JOURNAL   Mol Cell Biol 29 (19), 5339-5347 (2009)
   PUBMED   19651899
  REMARK    GeneRIF: TEB4 interacts with and mediates loss of type 2
            iodothyronine deiodinase (D2)activity, indicating that D2
            ubiquitination and degradation can be tissue specific, depending on
            WSB-1 and TEB4 expression levels.
REFERENCE   6  (bases 1 to 6258)
  AUTHORS   Kreft,S.G., Wang,L. and Hochstrasser,M.
  TITLE     Membrane topology of the yeast endoplasmic reticulum-localized
            ubiquitin ligase Doa10 and comparison with its human ortholog TEB4
            (MARCH-VI)
  JOURNAL   J Biol Chem 281 (8), 4646-4653 (2006)
   PUBMED   16373356
REFERENCE   7  (bases 1 to 6258)
  AUTHORS   Hassink,G., Kikkert,M., van Voorden,S., Lee,S.J., Spaapen,R., van
            Laar,T., Coleman,C.S., Bartee,E., Fruh,K., Chau,V. and Wiertz,E.
  TITLE     TEB4 is a C4HC3 RING finger-containing ubiquitin ligase of the
            endoplasmic reticulum
  JOURNAL   Biochem J 388 (Pt 2), 647-655 (2005)
   PUBMED   15673284
REFERENCE   8  (bases 1 to 6258)
  AUTHORS   Florea,L., Di Francesco,V., Miller,J., Turner,R., Yao,A.,
            Harris,M., Walenz,B., Mobarry,C., Merkulov,G.V., Charlab,R.,
            Dew,I., Deng,Z., Istrail,S., Li,P. and Sutton,G.
  TITLE     Gene and alternative splicing annotation with AIR
  JOURNAL   Genome Res 15 (1), 54-66 (2005)
   PUBMED   15632090
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On or before Mar 7, 2015 this sequence version replaced
            XM_215517.8, XM_001065952.5.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments and orthologous data.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643290.7632.1,
                                           SRR26643284.2733.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-225               JAXUCZ010000002.1  84243568-84243792   c
            226-322             JAXUCZ010000002.1  84221342-84221438   c
            323-396             JAXUCZ010000002.1  84220251-84220324   c
            397-540             JAXUCZ010000002.1  84217257-84217400   c
            541-613             JAXUCZ010000002.1  84214171-84214243   c
            614-782             JAXUCZ010000002.1  84211807-84211975   c
            783-972             JAXUCZ010000002.1  84209294-84209483   c
            973-1034            JAXUCZ010000002.1  84207042-84207103   c
            1035-1067           JAXUCZ010000002.1  84206133-84206165   c
            1068-1119           JAXUCZ010000002.1  84204255-84204306   c
            1120-1178           JAXUCZ010000002.1  84202683-84202741   c
            1179-1259           JAXUCZ010000002.1  84200729-84200809   c
            1260-1328           JAXUCZ010000002.1  84200437-84200505   c
            1329-1403           JAXUCZ010000002.1  84200279-84200353   c
            1404-1538           JAXUCZ010000002.1  84198994-84199128   c
            1539-1658           JAXUCZ010000002.1  84197754-84197873   c
            1659-1759           JAXUCZ010000002.1  84197457-84197557   c
            1760-1897           JAXUCZ010000002.1  84195355-84195492   c
            1898-2099           JAXUCZ010000002.1  84193586-84193787   c
            2100-2169           JAXUCZ010000002.1  84184706-84184775   c
            2170-2351           JAXUCZ010000002.1  84180755-84180936   c
            2352-2486           JAXUCZ010000002.1  84178872-84179006   c
            2487-2576           JAXUCZ010000002.1  84177393-84177482   c
            2577-2709           JAXUCZ010000002.1  84174954-84175086   c
            2710-2845           JAXUCZ010000002.1  84172279-84172414   c
            2846-6258           JAXUCZ010000002.1  84166570-84169982   c
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q23"
     gene            1..6258
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /note="membrane associated ring-CH-type finger 6"
                     /db_xref="GeneID:294862"
                     /db_xref="RGD:1565757"
     exon            1..225
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     CDS             207..2936
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /EC_number="2.3.2.27"
                     /note="E3 ubiquitin-protein ligase MARCH6;
                     membrane-associated ring finger (C3HC4) 6, E3 ubiquitin
                     protein ligase"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase MARCHF6"
                     /protein_id="NP_001292203.1"
                     /db_xref="GeneID:294862"
                     /db_xref="RGD:1565757"
                     /translation="MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWL
                     KHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFA
                     WLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISL
                     VWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPVGGNGAENPAADQPANPAGENAVL
                     GENPDAQDGQAEEEEEDNEEEDDAGVEDAADANNGAQDDMNWNALEWDRAAEELTWER
                     MLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLI
                     TTIVGYILLAITLIICHALATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGW
                     WLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGV
                     LWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSLLPH
                     FLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDL
                     HSYLLGDQEESENSANQQVNNNQPARNNNAVPAGEGLHAAHQAILQQGGPVGFQPYRR
                     PLNFPLRIFLLIVFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTAACGLY
                     VCWLTIRAVTVLVAWMPQGRRVIFQKVKEWSLMIMKTLIVAVLLAGVVPLLLGLLFEL
                     VIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRN
                     IDLHYIIRKLAAPVISVLLLSLCIPYVIASGVVPLLGVTAEMQNLVHRRIYPFLLMVV
                     VLMGILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGPSTPPPVSSQE"
     exon            226..322
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            323..396
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            397..540
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            541..613
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            614..782
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            783..972
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1034
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1067
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1119
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1178
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1259
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            1260..1328
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1403
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1538
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            1539..1658
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1759
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1897
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            1898..2099
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            2100..2169
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            2170..2351
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            2352..2486
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            2487..2576
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            2577..2709
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            2710..2845
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
     exon            2846..6258
                     /gene="Marchf6"
                     /gene_synonym="March6; RGD1310927; RGD1565757"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccccctcccc ggcgctcgct ttctgtcagc cgctgtccct ctccccctcg cccgagtctg
       61 gcttcctctc gcacctgagc gcaagcacct gccgggcccg cgtcctcttc ctcctcccct
      121 cctccctccc gcctttccct tcgggcccca ggctctgccg ggtcgcgggc gcctcgcggc
      181 tgcgtcaccg ccgcccccca aacaagatgg acaccgccga ggaagatata tgtagagtgt
      241 gtcggtcaga aggaacacct gagaaacctc tttaccatcc ttgtgtatgt actggcagta
      301 ttaagtttat tcatcaagaa tgcctagttc agtggttgaa acatagtcgg aaagaatact
      361 gtgaattatg caagcacaga tttgctttca caccaattta ttctccagat atgccttcac
      421 ggctaccaat ccaagacatt tttgctggac tggttacaag tattggcact gcgatacgat
      481 actggtttca ttacacactt gtggcctttg catggctggg agttgttcct cttacagcat
      541 gccgcatcta caagtgcttg tttactggct ccgtgagctc actactgaca ctgccactag
      601 acatgctgtc aacggaaaat ttgttggcgg attgcttgca gggttgtttt gtggtgacat
      661 gcacactttg tgcattcatc agcctggtgt ggttacgaga gcagatagtc catgggggag
      721 caccaatttg gctggagcat gctgctccac ccttcaatgc tgctggacac caccaaaatg
      781 aggctcctgt aggaggaaat ggagcagaaa accctgctgc tgatcagcca gctaacccag
      841 caggtgagaa tgcagttctg ggtgagaacc ctgatgccca ggatggtcaa gcagaagagg
      901 aggaggagga caatgaggag gaagatgatg caggtgtaga agatgctgct gatgccaaca
      961 atggggccca agacgacatg aactggaatg ctttggagtg ggaccgggct gctgaagagc
     1021 ttacttggga aagaatgctt ggacttgatg gatcactagt ttttctggaa catgtgttct
     1081 gggtggtatc gttaaacaca ctgttcattc ttgtttttgc attttgtccc taccacattg
     1141 gtcacttctc ccttgttggc ttggggtttg aggagcatgt tcaagcatct cattttgaag
     1201 gtctaatcac gacaattgtt ggatatatac ttttagcaat aacactgata atttgtcatg
     1261 ccttggccac tcttgttaaa tttcataggt ctcgtcgctt gctgggcgtc tgctatattg
     1321 tggttaaggt ctctctgtta gttgtggtag aaattggagt gttccctctc atctgtggct
     1381 ggtggctgga tatttgttct ctggaaatgt ttgatgctac tctgaaagat cgagagctga
     1441 gctttcagtc agccccaggg accacaatgt ttctgcactg gttggtggga atggtctatg
     1501 tgttctactt tgcctccttc atcctgttac tgagagaggt acttcgacct ggtgtcttat
     1561 ggtttctgag gaatttgaat gatccagatt ttaatccagt acaggaaatg attcacttgc
     1621 ctatttatag acatcttcga agatttattt tgtcagtgat tgtctttggc tccattgtac
     1681 ttctgatgct ctggcttcct atacgtataa ttaagagcct cttgcctcat tttcttcctt
     1741 acaatgtcat gctctacagt gatgctccgg tgagtgagct gtcccttgag ctgctcctgc
     1801 tgcaggttgt cttgccagcc ctgctggagc aggggcacac caggcagtgg ctgaaggggc
     1861 ttgttcgtgc atggactgtt actgctggat acctgctgga ccttcattcc tacttacttg
     1921 gagaccagga ggaaagtgag aacagtgcaa accagcaggt caacaacaat cagcctgcgc
     1981 ggaacaataa cgccgtccct gctggggaag gtctgcatgc agctcaccaa gccatactcc
     2041 agcagggagg acctgttggc tttcagcctt accgccggcc tttaaatttc ccactcagga
     2101 tatttctgct gattgtcttc atgtgcataa cattactaat tgccagcctc atctgcctta
     2161 ccttaccagt atttgctggc cgttggttaa tgtcattttg gacggggact gccaaaatcc
     2221 atgagctgta cacagctgct tgtggtctct atgtgtgttg gctaaccata agggctgtga
     2281 cggtgctggt ggcctggatg ccccaggggc ggagagtgat tttccagaag gtcaaagagt
     2341 ggtccctcat gataatgaag actttgatag ttgctgtgct gctggctggg gttgtcccac
     2401 tgctcctggg gctcctgttt gagctggtta ttgttgctcc tctgagggtc cctcttgatc
     2461 agactcccct tttctaccct tggcaggact gggcgctggg agttctccat gccaaaatca
     2521 tcgcagctat aacacttatg ggtcctcagt ggtggttgaa gactgtcatt gaacaggttt
     2581 acgcaaatgg catccgtaac atcgaccttc actatatcat tcgtaagctg gcagctccag
     2641 tgatctctgt gctcttgctc tccctgtgta taccgtatgt catagcttct ggcgtggttc
     2701 ctttattagg tgttactgca gaaatgcaga acttagttca ccggcggatt tatccatttt
     2761 tattgatggt cgttgtgctg atgggaatcc tgtccttcca ggtccgccag tttaagcgcc
     2821 tttatgaaca cattaaaaat gacaagtacc ttgtggggca gcgcctggtg aactatgagc
     2881 gcaaatctgg caagcaaggc ccatctacgc cacctccggt gtcgtcccaa gaataaagtg
     2941 cttgtctcag ctccttgagc ttctccttgc cttttcgtgt cctttgttgt ggacttctca
     3001 cttggagatt ctcctgtgat ctcagcgtgt ttaagttaaa tgtacttgac ttgtgtcctc
     3061 agcattcaga gagcagcgca tccggcacta ctgctcctcc ctgcatctgc cgacatcact
     3121 gctgtctgag atctgtacat gtgtaaatag gagttccttg atgccctaaa accttggatt
     3181 aaacagaatg tgcattgtac atctttaaca aatgtatatt aatttattaa atctagttgt
     3241 cactttattt tggacctgct gtgatctcga caggaaatgt gccacagagc agtagtgtat
     3301 aggcaagagc gagtgacatc tttcggagcg cagtgcatgg tgttgccagc agttctcacc
     3361 aggtcactgc atcctgccct tggctggtca gtccttacca gcagccgact gagcagtggg
     3421 gcctgtggta agggacacgg acagagtgcc tcacagacag tcgtgtttgg gaattattaa
     3481 atgtaacacc aaagctgttg tacattttct actgcctgat tgttttcatg tgtctgtgtt
     3541 tgtaatattg tatagtatgt tgtgctaggt gaggatatta cttttaattt tgataattta
     3601 atattcctag tgatcagcat tgggagttgg ggtttttgtg cttgttaggg gcatgtctat
     3661 ccttagaaag gtaaactaca aatgaagagt tttattagcc tcctcccctt ccacgcagct
     3721 ctttcttttt gtcagcagtg gccaaaagtg tcatgccaga tgagaaatcg gaggggatag
     3781 atagcatcac caacaccacc gttgtgagag gtggaggccc taggctgcgg tgtgaggctg
     3841 actgcatcag ggaggacgag cagaacatct gcagtgctgg cagcgcactg cagctgaggc
     3901 tcggtgaatg caccaggaag ccctcttcta aagcattttg tttacaataa actgcaagta
     3961 aaaccaagtt ttatttgttt gaaaggaagc agactgagct ggccttcacc ataggaaaga
     4021 gttgcattgg agaaatgctg tgagatttta gagaatttaa tactcagagg aagcctggtt
     4081 ccccctagcc caggcttcat gtttaaagtt taaaagatga ggatgattgt acatcgtcca
     4141 agtgtttaat ggtaaaggtg tattgtaagg atagtgctag aagctttcat agactctaaa
     4201 gagttccgtg gagtattaaa cccatagttg gaaaggtctg agggttcacc ccccacctcc
     4261 aaatacaaag ccaaacaaaa gattaagagc aatgcttctt ggttttgttc tctttttgat
     4321 tttgtctttt tgtcgttctt aaattcacta agtgataagc agatactttg ttttaaagca
     4381 gcattatatt aaacagaagc agtgcttgcc atgggatcca gtgttacact gtcattgtgt
     4441 gatggccttt ctcttgtgtg aagtatgaag taagggtctt ctagcaaatc taccttcact
     4501 ctcaggcttt gtccttccac agagaattag ccttttaaaa tgcagtgccg cctgtcaatg
     4561 ggaatatgtc tttgaaacct aatctcttga ggaaaaggat ggtggtgtat tgggcaaata
     4621 acatttaggc tttattttta atttttaaag aatttatgga tctaccacaa ggttaaaaat
     4681 tttaagtgaa ccttattaga tgttttgctg aaaacaaata caaacagtac agattaccaa
     4741 agtttgcccc ctttattggc agaggtctct gctctcttac tgaggaattc ctcctgggtt
     4801 tacagttgtg ttttgtctag tggctttcag taagataata tcctcatgag gatttattgt
     4861 gcataacatt tgctagggtg acatacaggt tttattcaac aatatcataa acatttaaaa
     4921 ccctcagttc agaaggtgtt cctatttgct gcctgtcttt acaggaagtt tacagataaa
     4981 gagttttgca tcgttttgaa ttgggctaac acccagtctt caaggaagct tacaaggaaa
     5041 gatctttgtg ctttattatc gataatttga agacaaatca ccacttggga ttagttttac
     5101 atgaccagtt atcaaatggt cactttgaaa tgtatagttg ttcgttatta acaaattatg
     5161 gtttgatttt aaactacaaa ctcctaatag tttagatgaa aactaaagaa aatgccaatg
     5221 taaaatttgt gtgtagctag ccttaaagtt ccatgtttat agttgtggtc ccagccccca
     5281 ctcctgttct ctggtggggg gaaggaggta ggatatgtga gcagtcattt ttatgtcagc
     5341 attcagtatg gttgttatta ctgtgaattc tcagacaggc cttcccctcg aggaacagag
     5401 ttattgtcct ccaatgagtt tattaggtgc attgtttttt tgttgcacaa atgcttcatt
     5461 agagtgtatt cttaaattta ggttgagctg atgaaatttt aagacaaaga caaaatatgc
     5521 attggacatt tgtaccctgt tgtgtttgca gacacaggag ccagtgccat tatgcaagaa
     5581 tttggcactt gtcacggtgc tccaaattag ctccttcctc tcctctctgg gatggagaat
     5641 atcaggctcg ttactgtgat gcctcattct acattgtgat gttcaaagtt cttgttaaaa
     5701 cagtcctcta ggatcgtacc tgttgagtca ttgtcaaggg gagcgaggga acatagtacc
     5761 gctggagtgt tacagaagac ctcacctgtg ggggctttac agtctttgct aactgcccag
     5821 tctccacaca cttaacttac cctgtcaatc agatgttttg tttcagctta ctgctgttgt
     5881 gtcaacattt aaacagctgc cgtgagcaag ggcttctttc agatactctc caataatagc
     5941 tttggcgtta atataaaaat aagacagtat acatgtatat gtatgtctcc attcctgtct
     6001 gtatgagtgc ctcattttgt aatcatgaca gtcctataga accttagatg gaaacgaatg
     6061 actaaggcct aggggtgtct gttgtaggaa tgaactagaa cacttagtga tgtgggatgt
     6121 cagttttcta ttacagtaaa cacctgtacc cagatcaatt ttatgtgtat atattcaaaa
     6181 atgctccctt ttttatgtgg tcatttgttg ccttctgagt taagagaaca gaattgaata
     6241 aagtgaatgt tagttgta
//
