LOCUS       NM_031622               4194 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Rattus norvegicus mitogen-activated protein kinase 6 (Mapk6), mRNA.
ACCESSION   NM_031622
VERSION     NM_031622.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Wang,R. and Xu,B.
  TITLE     TGF-beta1-modified MSC-derived exosomal miR-135b attenuates
            cartilage injury via promoting M2 synovial macrophage polarization
            by targeting MAPK6
  JOURNAL   Cell Tissue Res 384 (1), 113-127 (2021)
   PUBMED   33404840
  REMARK    GeneRIF: TGF-beta1-modified MSC-derived exosomal miR-135b
            attenuates cartilage injury via promoting M2 synovial macrophage
            polarization by targeting MAPK6.
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Zhang,Y., Su,Z., Liu,H.L., Li,L., Wei,M., Ge,D.J. and Zhang,Z.J.
  TITLE     Effects of miR-26a-5p on neuropathic pain development by targeting
            MAPK6 in in CCI rat models
  JOURNAL   Biomed Pharmacother 107, 644-649 (2018)
   PUBMED   30118880
  REMARK    GeneRIF: miR-26a-5p could act as a negative regulator of
            neuropathic pain development through targeting MAPK6
REFERENCE   3  (bases 1 to 4194)
  AUTHORS   Tan,J., Yang,L., Liu,C. and Yan,Z.
  TITLE     MicroRNA-26a targets MAPK6 to inhibit smooth muscle cell
            proliferation and vein graft neointimal hyperplasia
  JOURNAL   Sci Rep 7, 46602 (2017)
   PUBMED   28429763
  REMARK    GeneRIF: analysis revealed that the effects of miR-26a in VSMCs
            were mediated by targeting MAPK6 at the mRNA and protein levels.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4194)
  AUTHORS   Walder,R.Y., Wattiez,A.S., White,S.R., Marquez de Prado,B.,
            Hamity,M.V. and Hammond,D.L.
  TITLE     Validation of four reference genes for quantitative mRNA expression
            studies in a rat model of inflammatory injury
  JOURNAL   Mol Pain 10, 55 (2014)
   PUBMED   25187167
  REMARK    GeneRIF: Thsi study validated the Hprt1, Actb, Mapk6 or B2m and
            showed that any one or combination of two of them are good
            reference genes for normalization of mRNA expression in qPCR
            experiments in the spinal cord and RVM in the CFA model of
            inflammatory injury.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4194)
  AUTHORS   Parmar,M.S., Jaumotte,J.D., Wyrostek,S.L., Zigmond,M.J. and
            Cavanaugh,J.E.
  TITLE     Role of ERK1, 2, and 5 in dopamine neuron survival during aging
  JOURNAL   Neurobiol Aging 35 (3), 669-679 (2014)
   PUBMED   24411019
  REMARK    GeneRIF: It plays distinct role in proliferation, differentiation,
            and neuronal viability.
REFERENCE   6  (bases 1 to 4194)
  AUTHORS   Seternes,O.M., Mikalsen,T., Johansen,B., Michaelsen,E.,
            Armstrong,C.G., Morrice,N.A., Turgeon,B., Meloche,S., Moens,U. and
            Keyse,S.M.
  TITLE     Activation of MK5/PRAK by the atypical MAP kinase ERK3 defines a
            novel signal transduction pathway
  JOURNAL   EMBO J 23 (24), 4780-4791 (2004)
   PUBMED   15577943
  REMARK    Erratum:[EMBO J. 2005 Feb 23;24(4):873]
REFERENCE   7  (bases 1 to 4194)
  AUTHORS   Schumacher,S., Laass,K., Kant,S., Shi,Y., Visel,A., Gruber,A.D.,
            Kotlyarov,A. and Gaestel,M.
  TITLE     Scaffolding by ERK3 regulates MK5 in development
  JOURNAL   EMBO J 23 (24), 4770-4779 (2004)
   PUBMED   15538386
REFERENCE   8  (bases 1 to 4194)
  AUTHORS   Bind,E., Kleyner,Y., Skowronska-Krawczyk,D., Bien,E., Dynlacht,B.D.
            and Sanchez,I.
  TITLE     A novel mechanism for mitogen-activated protein kinase localization
  JOURNAL   Mol Biol Cell 15 (10), 4457-4466 (2004)
   PUBMED   15269285
  REMARK    GeneRIF: These data suggest a novel mechanism for the localization
            of an MAPK family member, ERK3, in which cell cycle-regulated,
            site-specific proteolysis generates the nuclear form of the
            protein.
REFERENCE   9  (bases 1 to 4194)
  AUTHORS   Coulombe,P., Rodier,G., Pelletier,S., Pellerin,J. and Meloche,S.
  TITLE     Rapid turnover of extracellular signal-regulated kinase 3 by the
            ubiquitin-proteasome pathway defines a novel paradigm of
            mitogen-activated protein kinase regulation during cellular
            differentiation
  JOURNAL   Mol Cell Biol 23 (13), 4542-4558 (2003)
   PUBMED   12808096
  REMARK    GeneRIF: ERK3 biological activity is regulated by its cellular
            abundance through the control of protein stability
REFERENCE   10 (bases 1 to 4194)
  AUTHORS   Boulton,T.G., Nye,S.H., Robbins,D.J., Ip,N.Y., Radziejewska,E.,
            Morgenbesser,S.D., DePinho,R.A., Panayotatos,N., Cobb,M.H. and
            Yancopoulos,G.D.
  TITLE     ERKs: a family of protein-serine/threonine kinases that are
            activated and tyrosine phosphorylated in response to insulin and
            NGF
  JOURNAL   Cell 65 (4), 663-675 (1991)
   PUBMED   2032290
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB742965.1, BC109380.1 and BE104187.1.
            
            On Nov 29, 2005 this sequence version replaced NM_031622.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC109380.1, SRR8487231.2287.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                CB742965.1         60-108
            50-4034             BC109380.1         1-3985
            4035-4194           BE104187.1         1-160               c
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q24"
     gene            1..4194
                     /gene="Mapk6"
                     /gene_synonym="ERK3"
                     /note="mitogen-activated protein kinase 6"
                     /db_xref="GeneID:58840"
                     /db_xref="RGD:62087"
     exon            1..145
                     /gene="Mapk6"
                     /gene_synonym="ERK3"
                     /inference="alignment:Splign:2.1.0"
     exon            146..1316
                     /gene="Mapk6"
                     /gene_synonym="ERK3"
                     /inference="alignment:Splign:2.1.0"
     CDS             762..2924
                     /gene="Mapk6"
                     /gene_synonym="ERK3"
                     /EC_number="2.7.11.24"
                     /note="ERK-3; p55-MAPK; extracellular signal-regulated
                     kinase 3; MAPK 6; MAP kinase 6"
                     /codon_start=1
                     /product="mitogen-activated protein kinase 6"
                     /protein_id="NP_113810.2"
                     /db_xref="GeneID:58840"
                     /db_xref="RGD:62087"
                     /translation="MAEKFESLMNIHGFDLGSRYMDLKPLGCGGNGLVFSAVDNDCDK
                     RVAIKKIVLTDPQSVKHALREIKIIRRLDHDNIVKVFEILGPSGSQLTDDVGSLTELN
                     SVYIVQEYMETDLANVLEQGPLLEEHARLFMYQLLRGLKYIHSANVLHRDLKPANLFI
                     NTEDLVLKIGDFGLARIMDPHYSHKGHLSEGLVTKWYRSPRLLLSPNNYTKAIDMWAA
                     GCIFAEMLTGKTLFAGAHELEQMQLILESIPVVHEEDRQELLSVIPVYIRNDMTEPHK
                     PLTQLLPGISREALDFLEQILTFSPMDRLTAEEALSHPYMSIYSFPTDEPISSHPFHI
                     EDEVDDILLMDETHSHIYNWERYHDCQFSEHDWPIHNNFDIDEVQLDPRALSDVTDEE
                     EVQVDPRKYLDGDREKYLEDPAFDTSYSAEPCWQYPDHHENKYCDLECSHTCNYKTRS
                     PSYLDNLVWRESEVNHYYEPKLIIDLSNWKEQSKDKSDKRGKSKCERNGLVKAQIALE
                     EASQQLAERERGQGFDFDAFIAGTVQLSAQRESADVVDKLNDLNSSVSQLEMKSLISK
                     SVSREKQEKGRANLAQLGALYQPSWESQFVSGGEECFLISQFCCEVRKDEHVEKENTY
                     TSYLDKFFSRKEDSEMLETEPVEEGKRGERGREAGLLSSGGEFLLSRQLESIGTPQFH
                     SPGGSPLKSIQATLTPSAMKSSPQIPHKTYSNILKHLN"
     exon            1317..1461
                     /gene="Mapk6"
                     /gene_synonym="ERK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1626
                     /gene="Mapk6"
                     /gene_synonym="ERK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1828
                     /gene="Mapk6"
                     /gene_synonym="ERK3"
                     /inference="alignment:Splign:2.1.0"
     exon            1829..4180
                     /gene="Mapk6"
                     /gene_synonym="ERK3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tctggcgggt cggggttaca tggcggcgac tgcggcaaag cgagagcctc ggaaacgccg
       61 ctgccgccag cacagccgga gacctgagcc gaccttgggg gctatccgcg ggccccgcac
      121 tctctcgatg agtcggagaa gtcccgttgt atcagagtaa gatggatggt agctttgact
      181 gtgactgtgg tgagctggag ccacctgatc actaagaaga gacacctgtc agccaacatc
      241 accagggctt cctgttgaaa cagcaacgag gaagcgagga agcggaggga acgagtgctt
      301 cccagcgcct taggatgctg ctcaggacca gtccaacact gaatgtatct gcactgtgag
      361 aggatgtcca cggaggcctg ccgggcgcat atttattcac agttttgtcc catgttaagt
      421 cggttagcat agtgaatctg agtgcatagt atgtcatttc attccgttga gtttctcgag
      481 tgttttcttt aaatgtctgc agagtcgcta cccttccttg aactatgagc actgcaatct
      541 tcttaattct cagtatgaag agagattttt gagctttaag tctgagggga actcagcagg
      601 cctggttggc gtctgcaatg aacatcaaga aaccatcgtg ctgtgggaat gtgatcgttt
      661 ttctcccttt ttgagagatc tttccttttg atgccagttt tcttccttgt ttacacaagt
      721 tcaacaattt gaaaggaaaa ggcaattgta agggttttaa aatggcagag aaatttgaaa
      781 gtctcatgaa cattcatggc tttgatctgg gttccaggta catggactta aaaccattgg
      841 gctgtggagg caatggcttg gttttttctg ctgtagacaa tgactgtgac aaaagagtag
      901 ccatcaagaa aattgtcctc accgatcccc agagtgtcaa acatgccctc cgtgaaatca
      961 aaattattag aagacttgac cacgataaca ttgtgaaagt gtttgaaatt cttggtccca
     1021 gtggaagcca gctgacagac gatgtgggct ctctaacaga gctgaatagc gtctacattg
     1081 ttcaggagta catggagaca gacttggcga acgtgctgga gcagggccct ttactggagg
     1141 agcatgccag gctcttcatg taccagctgc tgcgtgggct caagtacatc cactctgcaa
     1201 acgtgctgca cagggatctc aagccggcca accttttcat taacactgaa gacttggtgc
     1261 tgaagattgg tgactttggc ctggcccgga tcatggatcc tcattattcc cataagggtc
     1321 atctttctga aggattggtt accaaatggt acagatctcc acggctttta ctttctccta
     1381 ataactatac taaagccatt gacatgtggg ctgcaggctg catctttgct gaaatgctga
     1441 ctggtaaaac cctctttgca ggtgcacatg aacttgaaca gatgcagctg atcttggagt
     1501 ctatccctgt tgtgcacgag gaagatcggc aggagcttct cagcgtgatt ccagtttaca
     1561 ttagaaacga catgactgag ccacacaaac cgctgactca gctgcttccg gggattagtc
     1621 gggaagcact ggatttcctg gaacagattc tgacgttcag tcccatggac cggctgacag
     1681 ccgaggaagc actttcccat ccttacatga gcatctactc tttcccaacg gacgagccta
     1741 tttccagcca tcctttccac atagaagacg aagtggacga cattttgcta atggatgaaa
     1801 cacacagtca catttataac tgggaaaggt accacgattg tcagttctcg gagcatgact
     1861 ggcctattca taacaacttt gatatcgatg aggttcagct tgacccgaga gctctgtctg
     1921 atgtcaccga tgaagaagaa gttcaagttg atcctcgaaa gtacttggat ggagaccgag
     1981 agaagtatct ggaggatccc gccttcgaca ccagctactc tgctgagcct tgctggcagt
     2041 acccagatca ccacgagaac aagtactgtg atctggagtg tagccacacc tgtaactaca
     2101 aaacaaggtc gccatcatac ttagataacc tggtgtggag ggagagcgag gttaaccatt
     2161 actatgagcc caagcttatt atagatcttt ccaactggaa agagcaaagt aaggacaaat
     2221 ccgacaagag aggcaagtcc aagtgtgaga ggaacgggtt ggtcaaggcg cagattgcgc
     2281 ttgaggaagc gtcccagcag ctggctgaga gggagagggg ccaaggcttt gactttgatg
     2341 ccttcatcgc aggcaccgtt cagctcagtg cccagcgtga gtctgctgac gtagttgaca
     2401 agttaaacga cttgaatagc tcagtgtccc agctagaaat gaaaagcctg atatccaagt
     2461 cagtcagccg agaaaagcaa gaaaagggaa gggctaacct ggcccagctg ggagccttgt
     2521 accagccctc ctgggagagc cagtttgtga gtggcgggga ggagtgcttc cttatcagtc
     2581 agttttgttg tgaggtcagg aaggacgaac acgtggagaa ggagaacact tacaccagct
     2641 atttggacaa gttttttagc aggaaggagg attctgaaat gctagaaact gagccagtgg
     2701 aagaagggaa gcgtggggag agaggccgtg aggcagggct tctgagcagc ggtggggagt
     2761 ttctcctgag caggcagcta gagtccatag gcaccccgca gttccacagt ccagggggat
     2821 ccccactcaa gtccatccag gccacgttaa caccttccgc tatgaaatct tcccctcaaa
     2881 tccctcacaa gacatacagc aacattctga aacatctgaa ctaaacactc agcagacact
     2941 tctttgttct tcatgaaatg tgttgtgtct tttttatcac taatgtttta agtcattttt
     3001 tttttacttg aatcagaagg tgtcattaat ttgcaaggat ttttcttggt tctcagtttg
     3061 taaaacacag agttttttct acatgtgagt tagttttcat ttgaactggc atgtcgtttg
     3121 cacacacaca aagaatagag caaaacaatg cagtgcagga ggagacaaga tgcgctagga
     3181 tggacagaca ttctcacaga ccagtgacct gcttacagga aacaaaacct tgccttgaaa
     3241 cttacacagt gagactgtac ataattgcat gaaaagatct atttttttcc tgaaacattt
     3301 ttcattcatt agtattttca agtttttcat actgtacaca tttcttaaga cacatgatac
     3361 cagcagcaac tgaaaacgaa tgccgaattt ggtacacatg tgttatctac ctcaaggtaa
     3421 caaaagtatg cggcgaaacc taacccaccc atagtgctcc gcggcatatg cacttgtatc
     3481 tagccagcgt tggccgcagt aaccaatgag actcgtccgc catttatcaa tgtcctggtg
     3541 ttcatccttt acagtgaagt gttagataca tcacatctta tttattttta gcaaatcagt
     3601 atattttctg tatttaatta taaaaggtaa cttagtttaa gtttatttgc aactgccctt
     3661 cttcccgttt ggcactatgg tttgttgcct gccgagctga tctgagaagt cagcttgtcc
     3721 cgaggctgtc catgtacgtt aagtaaagtg ctcactgtgt ataggaatct gtattttgga
     3781 ggtgcttgat ctatctacaa agaaaaaaat taggaattta ttataaaatg ctcctagaag
     3841 tcttaatggt gtttattttt taaaaccttg taatgttaga cttgtgtgca tggaagtgat
     3901 taaggtacat cattattgta gtttgaacat tgtacatgat aagccttccc ccacccccgt
     3961 ttttactgta tgtttttatt gaatgatcta ttccccatcc ctaggcaagc atgaataaaa
     4021 ttaggttaaa tgtagcatgt cactgctcag tcttttagag ttgtttcgtc tgttttattt
     4081 tgctgaatcc tgtctgtatc tttggttatc ctctttgaag gaaaaagaaa aataaagatg
     4141 gccagcaggc acggctcctg tttgactctt tagaaggaaa aaaaaaaaaa aaaa
//
