LOCUS       NM_001401453            5441 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus mitogen activated protein kinase kinase kinase 4
            (Map3k4), transcript variant 1, mRNA.
ACCESSION   NM_001401453 XM_039110556
VERSION     NM_001401453.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Huang,L. and Wang,L.
  TITLE     Upregulation of miR-183 represses neuropathic pain through
            inhibiton of MAP3K4 in CCI rat models
  JOURNAL   J Cell Physiol 235 (4), 3815-3822 (2020)
   PUBMED   31602666
  REMARK    GeneRIF: Upregulation of miR-183 represses neuropathic pain through
            inhibiton of MAP3K4 in CCI rat models.
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Cerone,M.A., Burgess,D.J., Naceur-Lombardelli,C., Lord,C.J. and
            Ashworth,A.
  TITLE     High-throughput RNAi screening reveals novel regulators of
            telomerase
  JOURNAL   Cancer Res 71 (9), 3328-3340 (2011)
   PUBMED   21531765
REFERENCE   3  (bases 1 to 5441)
  AUTHORS   Cuadrado,A. and Nebreda,A.R.
  TITLE     Mechanisms and functions of p38 MAPK signalling
  JOURNAL   Biochem J 429 (3), 403-417 (2010)
   PUBMED   20626350
  REMARK    Review article
REFERENCE   4  (bases 1 to 5441)
  AUTHORS   Abell,A.N., Granger,D.A., Johnson,N.L., Vincent-Jordan,N.,
            Dibble,C.F. and Johnson,G.L.
  TITLE     Trophoblast stem cell maintenance by fibroblast growth factor 4
            requires MEKK4 activation of Jun N-terminal kinase
  JOURNAL   Mol Cell Biol 29 (10), 2748-2761 (2009)
   PUBMED   19289495
REFERENCE   5  (bases 1 to 5441)
  AUTHORS   Johnson,G.L. and Nakamura,K.
  TITLE     The c-jun kinase/stress-activated pathway: regulation, function and
            role in human disease
  JOURNAL   Biochim Biophys Acta 1773 (8), 1341-1348 (2007)
   PUBMED   17306896
  REMARK    Review article
REFERENCE   6  (bases 1 to 5441)
  AUTHORS   Luo,W., Ng,W.W., Jin,L.H., Ye,Z., Han,J. and Lin,S.C.
  TITLE     Axin utilizes distinct regions for competitive MEKK1 and MEKK4
            binding and JNK activation
  JOURNAL   J Biol Chem 278 (39), 37451-37458 (2003)
   PUBMED   12878610
REFERENCE   7  (bases 1 to 5441)
  AUTHORS   Takekawa,M. and Saito,H.
  TITLE     A family of stress-inducible GADD45-like proteins mediate
            activation of the stress-responsive MTK1/MEKK4 MAPKKK
  JOURNAL   Cell 95 (4), 521-530 (1998)
   PUBMED   9827804
REFERENCE   8  (bases 1 to 5441)
  AUTHORS   Takekawa,M., Posas,F. and Saito,H.
  TITLE     A human homolog of the yeast Ssk2/Ssk22 MAP kinase kinase kinases,
            MTK1, mediates stress-induced activation of the p38 and JNK
            pathways
  JOURNAL   EMBO J 16 (16), 4973-4982 (1997)
   PUBMED   9305639
REFERENCE   9  (bases 1 to 5441)
  AUTHORS   Fanger,G.R., Johnson,N.L. and Johnson,G.L.
  TITLE     MEK kinases are regulated by EGF and selectively interact with
            Rac/Cdc42
  JOURNAL   EMBO J 16 (16), 4961-4972 (1997)
   PUBMED   9305638
REFERENCE   10 (bases 1 to 5441)
  AUTHORS   Washburn,L.L. and Eicher,E.M.
  TITLE     Sex reversal in XY mice caused by dominant mutation on chromosome
            17
  JOURNAL   Nature 303 (5915), 338-340 (1983)
   PUBMED   6855886
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Jan 3, 2023 this sequence version replaced NM_001401453.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643296.456.1, SRR26643298.893.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-284               JAXUCZ010000001.1  50979600-50979883
            285-475             JAXUCZ010000001.1  51016396-51016586
            476-1833            JAXUCZ010000001.1  51023727-51025084
            1834-2076           JAXUCZ010000001.1  51032441-51032683
            2077-2223           JAXUCZ010000001.1  51035050-51035196
            2224-2381           JAXUCZ010000001.1  51036600-51036757
            2382-2498           JAXUCZ010000001.1  51039214-51039330
            2499-2598           JAXUCZ010000001.1  51040488-51040587
            2599-2682           JAXUCZ010000001.1  51040671-51040754
            2683-2949           JAXUCZ010000001.1  51042059-51042325
            2950-3099           JAXUCZ010000001.1  51043433-51043582
            3100-3261           JAXUCZ010000001.1  51045179-51045340
            3262-3395           JAXUCZ010000001.1  51045792-51045925
            3396-3474           JAXUCZ010000001.1  51046913-51046991
            3475-3541           JAXUCZ010000001.1  51047670-51047736
            3542-3638           JAXUCZ010000001.1  51049989-51050085
            3639-3800           JAXUCZ010000001.1  51050780-51050941
            3801-3901           JAXUCZ010000001.1  51053675-51053775
            3902-3982           JAXUCZ010000001.1  51054303-51054383
            3983-4174           JAXUCZ010000001.1  51055627-51055818
            4175-4245           JAXUCZ010000001.1  51056785-51056855
            4246-4362           JAXUCZ010000001.1  51057882-51057998
            4363-4522           JAXUCZ010000001.1  51058694-51058853
            4523-4645           JAXUCZ010000001.1  51060713-51060835
            4646-4752           JAXUCZ010000001.1  51061223-51061329
            4753-4932           JAXUCZ010000001.1  51065197-51065376
            4933-5441           JAXUCZ010000001.1  51066609-51067117
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q11"
     gene            1..5441
                     /gene="Map3k4"
                     /note="mitogen activated protein kinase kinase kinase 4"
                     /db_xref="GeneID:308106"
                     /db_xref="RGD:1311192"
     exon            1..284
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     CDS             154..4953
                     /gene="Map3k4"
                     /EC_number="2.7.11.25"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase 4
                     isoform 1"
                     /protein_id="NP_001388382.1"
                     /db_xref="GeneID:308106"
                     /db_xref="RGD:1311192"
                     /translation="MRDAIAEPVPPPALAVNPAAAMEEPRPAPPPQPEPESECCPAAR
                     QEHMLGESARKSLESDPEDFSDETNTENLYGTSPPSTPRQMKRLSAKHQRNSAGRPAS
                     RANLKEKMNTPSQSPHKDLGKGVEAVEEYSYKQGKKTRATLRTTERDHKKNAQCSFML
                     DSVAGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSMPMPIARTARQTSRTDCPADRLKF
                     FETLRLLLKLTSVSKKKDREQRGQENTAAFWFNRSNELIWLELQAWHAGRTINDQDLF
                     LYTARQAIPDIINEILTFKVNYGSAAFSRNGAGFNGTSLEGQCRTLHGTNSLGCSTYH
                     EHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYERYAAKDFEDRVQALCLWLNI
                     TKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGYEPEEEGEDTEGELRELESG
                     TEESDEERSPNPRVPELRLSADTILDSHSQGCASRKLERLESEEDSVGWGIADCGPEA
                     SRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMNGSLQRARVALVKDDRPVEFSDFPG
                     PMWGSDYVQLSGTPPSSEQKCSAVSWEELRAMDLPSFEPAFLVLCRVLLNVIHECLKL
                     RLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLQEVLDGLEKTDCNMDAFE
                     DDLQKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGK
                     LFCDIAGMLLKSTGSFLDSGLQESCAELWISADDNGAADELRRSVIEISRALKELFHE
                     ARERASKALGFAKMLRKDLEIAAEFVLSASARELLDVLRAKQYVKVQIPGLENLHVFV
                     PDSLAEEKKIILQLLNAATGKDCSKDPDDVFVDAFLLLTKHGDRARDSEDGWSTWEAR
                     AIKIVPQVETVDTLRSMQVDNLLLVVMESAHLVLQRKAFQQSIEGLMTVHHEQTSSQP
                     VIAKALQQLKNDALELCNRISDAIDRVDHMFTLELDAEVEESESATLQQYYREAMIQG
                     YNFGFEYHKEVVRLMSGEFRQKIGDKYISFAQKWMNYVLTKCESGRGTRPRWATQGFD
                     FLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHS
                     DPPNPHLIIPTPEGFSTRSVPSDTRNHGNSVAAAAVAAAAAAATTAAGRPGPGGGDSA
                     PAKPVNTAPDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPRSDSSGST
                     RRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEERRYREMRRKNIIGQVCDTPKSYD
                     NIMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKET
                     ADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSK
                     QITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTL
                     GTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEFEHNFQIMYKVGMG
                     HKPPIPERLSPEGKDFLSHCLESDPKIRWTASQLLDHAFVKVCTDEE"
     exon            285..475
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            476..1833
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..2076
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            2077..2223
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            2224..2381
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            2382..2498
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            2499..2598
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            2599..2682
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            2683..2949
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            2950..3099
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            3100..3261
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            3262..3395
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            3396..3474
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            3475..3541
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            3542..3638
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            3639..3800
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            3801..3901
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            3902..3982
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            3983..4174
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            4175..4245
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            4246..4362
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            4363..4522
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            4523..4645
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            4646..4752
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            4753..4932
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
     exon            4933..5441
                     /gene="Map3k4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gccgttccca agatggctgc tgcgcgcacg gctcctgcgg cgggctagag gcggaggcgg
       61 agtcgagtca ctccctcacc ccgcggctcc aggtcttccc gcaccaggct gcagctgacg
      121 acccgccgcg gtcatgcgga gcttggtgca cggatgagag acgccatcgc cgagccggtg
      181 ccccctcccg ccctcgccgt caaccctgca gccgccatgg aggagccgcg gccagcaccg
      241 ccgccacaac ccgagccgga gtcggagtgc tgcccagcgg cgaggcagga gcacatgtta
      301 ggagagtcag ctcgaaagag tctggaatct gatccagagg acttctctga tgaaacgaat
      361 acagagaatc tttacggcac ctcaccccca agcacacctc gacagatgaa acgcctgtca
      421 gccaagcacc agaggaacag tgcggggagg ccagccagcc gagcaaactt gaaagaaaaa
      481 atgaacacac cgagtcagtc tccacataaa gacttgggga agggagtgga ggccgtggag
      541 gagtacagct acaagcaggg gaagaagacg cgagcgactc tgaggacaac agagcgagac
      601 cataagaaaa atgcacagtg ctcgttcatg ttggactcag tggccgggtc tttgccaaaa
      661 aaatctattc cagatgtgga tctcaataag ccttacctca gcctcggctg tagcaacgcc
      721 aagctgccag tctctatgcc catgcctata gccagaactg cgcggcagac ttcccggacg
      781 gactgcccag cggatcggtt aaagttcttt gaaacactgc gccttttgct aaagcttacc
      841 tcagtctcga agaagaagga cagggaacag aggggacagg aaaacacggc tgctttctgg
      901 ttcaaccggt caaatgaact gatctggtta gagctccagg cctggcacgc aggccgcacc
      961 atcaatgacc aggacctctt tttatacaca gcccgccaag ccatcccaga tattatcaat
     1021 gaaatcctca cattcaaagt taattatgga agcgctgcct tctctagaaa tggagctggt
     1081 ttcaacggta cctcgctaga aggtcagtgc agaacccttc atgggacgaa cagtctaggc
     1141 tgttcaacct accacgagca cctccagcgc cagagggtct catttgagca ggtgaaacgg
     1201 ataatggagc tgctagagta catggaagca ctttatccgt ccttgcaggc tctgcagaag
     1261 gactacgaga gatacgccgc aaaggacttt gaggacagag tgcaggcact ctgcctgtgg
     1321 ctaaacatca cgaaagattt aaatcagaag ttgaggatta tgggcactgt tctgggcatc
     1381 aagaacttat cagacattgg ctggccagtg tttgaaatcc cttcccctcg gccatccaaa
     1441 ggctacgagc ctgaggagga gggcgaggac acagagggag agctgaggga gctggagagt
     1501 gggacggagg agagtgatga ggagcgaagt cccaatccga gggtgccaga gctcaggctg
     1561 tctgccgaca ccatcttgga cagtcactcc cagggctgcg cgtccaggaa acttgagagg
     1621 ctcgagtccg aggaggactc tgtaggctgg gggatagcag actgtggtcc cgaagccagt
     1681 aggcattgtt tgacttctat ctatagaccg ttcgtggaca aagcactgaa gcaaatgggg
     1741 ctgaggaagt taattttacg acttcataag ctaatgaatg gctccttgca aagggctcgt
     1801 gtagctctgg taaaggatga ccgtcctgtg gagttctctg actttccagg tcccatgtgg
     1861 ggctcggatt atgtgcagtt gtcggggacg cctccttcct cggagcagaa gtgcagtgct
     1921 gtgtcctggg aagagctgag ggccatggac ctgccctcct ttgagcccgc cttcctggtg
     1981 ctctgccggg tcctgctgaa cgtgatccat gagtgcctga agctgcggct ggaacagagg
     2041 ccggccgggg agccttccct cttgagtatc aagcagcttg tgcgagagtg taaagaggtg
     2101 ctgaagggcg ggctcctgat gaagcagtat taccagttca tgctgcagga ggtcctggac
     2161 ggcttggaga agaccgactg caacatggat gctttcgagg atgacctgca gaagatgctg
     2221 atggtgtatt ttgactacat gagaagctgg atccaaatgc tacagcagtt acctcaggct
     2281 tcccatagct taaaaaacct gctagaagaa gaatggaatt tcaccaaaga aataacccat
     2341 tacatccggg gaggagaagc acaggctgga aagcttttct gtgacattgc agggatgctg
     2401 ctgaaatcca cggggagctt tctggactct ggcctacagg agagctgtgc tgaactgtgg
     2461 atcagcgccg acgacaacgg tgctgccgac gagctaagga ggtctgtcat cgagatcagc
     2521 cgagcactca aggagctctt ccatgaggcc cgggaaagag cctccaaggc cctgggcttt
     2581 gctaaaatgc tgaggaagga cctagaaata gcagcagagt ttgttctgtc tgcgtctgcc
     2641 cgagagctcc tagacgttct gagagcaaag cagtatgtta aggtacagat tcctgggcta
     2701 gagaatttgc acgtgtttgt ccccgacagc ctcgctgagg agaagaaaat tattttgcag
     2761 ctactcaatg ctgccacagg aaaggactgc tcaaaggatc cggacgacgt ctttgtggat
     2821 gccttcctgc tcctgaccaa gcatggggac cgagcccgtg actcagaaga tggctggagc
     2881 acatgggaag cacgagctat caaaattgtg cctcaggtgg agaccgtgga caccctgaga
     2941 agcatgcagg tggacaacct tctgctggtt gtcatggagt ctgctcacct cgtacttcag
     3001 agaaaggcct tccagcagtc cattgagggg ctgatgactg tacaccatga gcagacatcc
     3061 agccagccag tcattgccaa agctttacag cagctaaaga acgatgcgct cgagctgtgc
     3121 aacagaataa gcgatgccat cgaccgcgtg gaccacatgt tcacgctgga gttggatgcc
     3181 gaggtcgagg agtccgagtc ggccacgctg cagcagtact accgagaggc catgattcag
     3241 ggctacaact tcgggtttga gtatcataaa gaagttgttc gtttgatgtc tggagaattc
     3301 agacagaaga taggagacaa atatataagc tttgcccaga agtggatgaa ttatgtgctg
     3361 accaaatgtg agagcggcag aggcacaaga cccagatggg ccacccaagg ttttgatttc
     3421 ctacaagcca ttgaacctgc ctttatttca gctttaccag aagatgactt cttgagtttg
     3481 caagccctga tgaatgagtg cattgggcac gtcataggaa agccacacag ccctgtcaca
     3541 gctatccatc ggaacagccc ccgccctgtg aaggtgcccc gatgccacag tgaccctcct
     3601 aaccctcacc tcatcatccc aactccagag ggattcagca cccggagcgt gccttccgac
     3661 actcggaacc atggcaactc tgttgctgct gctgctgttg ctgctgccgc cgccgccgcc
     3721 accactgctg ctggccgccc tggcccaggt ggtggtgact ctgcgccggc caaacctgtc
     3781 aacactgccc ctgataccag gggttccagc gtccctgaaa atgaccgctt ggcctccata
     3841 gctgcagaac tgcagttcag gtctctgagt cggcactcga gccccacgga agagcgagac
     3901 gagccagcat atcctcggag cgactcaagt ggatcaacac ggagaagctg ggaacttcga
     3961 acactcatca gccagaccaa agactcggcc tctaagcagg ggcccataga ggccatccag
     4021 aagtcagtcc gactgtttga agagaggagg tatcgagaga tgaggaggaa gaatatcatc
     4081 ggtcaagtgt gcgatacccc taagtcctat gataacatca tgcatgttgg actgaggaag
     4141 gtgacgttta agtggcaaag aggaaacaaa atcggagaag ggcagtatgg aaaagtgtac
     4201 acctgcatca gcgtcgacac aggggagctg atggccatga aggagattcg atttcagcct
     4261 aacgaccata agactatcaa ggaaactgca gacgagttga aaatatttga aggcatcaag
     4321 caccccaacc tggtccggta ctttggtgtg gagcttcaca gggaagagat gtacatcttc
     4381 atggagtact gtgatgaagg cacactagag gaggtgtcaa ggctgggcct tcaggagcac
     4441 gtcatccggt tgtattccaa gcagatcact gtcgccatca acgtcctcca tgagcatggc
     4501 atcgttcacc gagacatcaa aggtgccaat atcttcctta catcgtctgg actaatcaaa
     4561 ctgggagatt ttggatgctc agtgaaactt aaaaacaatg ctcagaccat gcctggcgag
     4621 gtgaacagca ccctagggac agcagcttac atggcccctg aagttattac ccgagctaaa
     4681 ggagaaggcc acggacgtgc ggcagacatc tggagtctgg ggtgcgtcgt catagagatg
     4741 gtgactggca agcggccttg gcatgagttt gaacacaact ttcagattat gtacaaggtg
     4801 ggaatgggac acaagccacc aatccccgaa aggttaagcc ctgaaggaaa ggactttctc
     4861 tcgcactgcc tggaaagtga cccgaagata aggtggacag ccagccagct ccttgaccac
     4921 gcttttgtca aggtttgcac agatgaagag tgaagctcta gtccgtggcc tagcagtgtg
     4981 tggacagaat cccgtggtca ctactgtatg taatatttac ataaagactg cagtgcaggc
     5041 ggcctcttaa ccttccaaga ctgaagacta cacggtgaca agcctcactc ctgctgctcc
     5101 tggtgtctgc aatagcacag gacccccgag tgacagtgtt gacaaggtta aagaagctgc
     5161 atgttaagtg ccattactac tgtacacgga ccaccgcctc tgtctcctcc gttccccgtg
     5221 tgacatcagc atagtgttct tgagcttctg ggttcagaag aaaatgtagt gttaatcagg
     5281 tgtccaggac cttgtttccg cctcctgttt gttggctctg actgtggctc cgactgtgga
     5341 gcctccttgt tggcaagctg caggtttgtt atgcaaaggt tcataagtga aactgaagaa
     5401 aaggttcttt ttcaataaat ggtttatttt aggaaagcta a
//
