LOCUS       NM_001398993            3237 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus mitogen-activated protein kinase kinase kinase 20
            (Map3k20), transcript variant 1, mRNA.
ACCESSION   NM_001398993 XM_039106371
VERSION     NM_001398993.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Spielmann,M., Kakar,N., Tayebi,N., Leettola,C., Nurnberg,G.,
            Sowada,N., Lupianez,D.G., Harabula,I., Flottmann,R., Horn,D.,
            Chan,W.L., Wittler,L., Yilmaz,R., Altmuller,J., Thiele,H., van
            Bokhoven,H., Schwartz,C.E., Nurnberg,P., Bowie,J.U., Ahmad,J.,
            Kubisch,C., Mundlos,S. and Borck,G.
  TITLE     Exome sequencing and CRISPR/Cas genome editing identify mutations
            of ZAK as a cause of limb defects in humans and mice
  JOURNAL   Genome Res 26 (2), 183-191 (2016)
   PUBMED   26755636
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Hsieh,Y.L., Tsai,Y.L., Shibu,M.A., Su,C.C., Chung,L.C., Pai,P.,
            Kuo,C.H., Yeh,Y.L., Viswanadha,V.P. and Huang,C.Y.
  TITLE     ZAK induces cardiomyocyte hypertrophy and brain natriuretic peptide
            expression via p38/JNK signaling and GATA4/c-Jun transcriptional
            factor activation
  JOURNAL   Mol Cell Biochem 405 (1-2), 1-9 (2015)
   PUBMED   25869677
  REMARK    GeneRIF: The role of ZAK and/or the ZAK downstream events such as
            JNK and p38 phosphorylation, c-Jun, and GATA-4 nuclear
            translocation in cardiac hypertrophy.
REFERENCE   3  (bases 1 to 3237)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   4  (bases 1 to 3237)
  AUTHORS   Cuadrado,A. and Nebreda,A.R.
  TITLE     Mechanisms and functions of p38 MAPK signalling
  JOURNAL   Biochem J 429 (3), 403-417 (2010)
   PUBMED   20626350
  REMARK    Review article
REFERENCE   5  (bases 1 to 3237)
  AUTHORS   Cheng,Y.C., Kuo,W.W., Wu,H.C., Lai,T.Y., Wu,C.H., Hwang,J.M.,
            Wang,W.H., Tsai,F.J., Yang,J.J., Huang,C.Y. and Chu,C.H.
  TITLE     ZAK induces MMP-2 activity via JNK/p38 signals and reduces MMP-9
            activity by increasing TIMP-1/2 expression in H9c2 cardiomyoblast
            cells
  JOURNAL   Mol Cell Biochem 325 (1-2), 69-77 (2009)
   PUBMED   19184368
  REMARK    GeneRIF: ZAK activity inhibition may be a good strategy to prevent
            the cardiac fibrosis progression.
REFERENCE   6  (bases 1 to 3237)
  AUTHORS   Tosti,E., Waldbaum,L., Warshaw,G., Gross,E.A. and Ruggieri,R.
  TITLE     The stress kinase MRK contributes to regulation of DNA damage
            checkpoints through a p38gamma-independent pathway
  JOURNAL   J Biol Chem 279 (46), 47652-47660 (2004)
   PUBMED   15342622
REFERENCE   7  (bases 1 to 3237)
  AUTHORS   Gross,E.A., Callow,M.G., Waldbaum,L., Thomas,S. and Ruggieri,R.
  TITLE     MRK, a mixed lineage kinase-related molecule that plays a role in
            gamma-radiation-induced cell cycle arrest
  JOURNAL   J Biol Chem 277 (16), 13873-13882 (2002)
   PUBMED   11836244
REFERENCE   8  (bases 1 to 3237)
  AUTHORS   Gotoh,I., Adachi,M. and Nishida,E.
  TITLE     Identification and characterization of a novel MAP kinase kinase
            kinase, MLTK
  JOURNAL   J Biol Chem 276 (6), 4276-4286 (2001)
   PUBMED   11042189
REFERENCE   9  (bases 1 to 3237)
  AUTHORS   Liu,T.C., Huang,C.J., Chu,Y.C., Wei,C.C., Chou,C.C., Chou,M.Y.,
            Chou,C.K. and Yang,J.J.
  TITLE     Cloning and expression of ZAK, a mixed lineage kinase-like protein
            containing a leucine-zipper and a sterile-alpha motif
  JOURNAL   Biochem Biophys Res Commun 274 (3), 811-816 (2000)
   PUBMED   10924358
REFERENCE   10 (bases 1 to 3237)
  AUTHORS   Zwacka,R.M., Reuter,A., Pfaff,E., Moll,J., Gorgas,K., Karasawa,M.
            and Weiher,H.
  TITLE     The glomerulosclerosis gene Mpv17 encodes a peroxisomal protein
            producing reactive oxygen species
  JOURNAL   EMBO J 13 (21), 5129-5134 (1994)
   PUBMED   7957077
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Dec 29, 2021 this sequence version replaced XM_039106371.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360186.254217.1,
                                           SRR26643285.11869.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-125               JAXUCZ010000003.1  77538146-77538270
            126-318             JAXUCZ010000003.1  77550298-77550490
            319-406             JAXUCZ010000003.1  77605187-77605274
            407-508             JAXUCZ010000003.1  77613431-77613532
            509-574             JAXUCZ010000003.1  77617181-77617246
            575-603             JAXUCZ010000003.1  77621141-77621169
            604-741             JAXUCZ010000003.1  77621285-77621422
            742-828             JAXUCZ010000003.1  77627986-77628072
            829-903             JAXUCZ010000003.1  77633633-77633707
            904-1010            JAXUCZ010000003.1  77638793-77638899
            1011-1146           JAXUCZ010000003.1  77647804-77647939
            1147-1191           JAXUCZ010000003.1  77659462-77659506
            1192-1222           JAXUCZ010000003.1  77661162-77661192
            1223-1362           JAXUCZ010000003.1  77661286-77661425
            1363-1425           JAXUCZ010000003.1  77666493-77666555
            1426-1518           JAXUCZ010000003.1  77667891-77667983
            1519-1635           JAXUCZ010000003.1  77687798-77687914
            1636-1710           JAXUCZ010000003.1  77690199-77690273
            1711-1861           JAXUCZ010000003.1  77693243-77693393
            1862-3237           JAXUCZ010000003.1  77696165-77697540
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q23"
     gene            1..3237
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /note="mitogen-activated protein kinase kinase kinase 20"
                     /db_xref="GeneID:311743"
                     /db_xref="RGD:1561394"
     exon            1..125
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            126..318
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     CDS             160..2568
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /EC_number="2.7.11.25"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mitogen-activated protein kinase kinase kinase MLT;
                     sterile alpha motif and leucine zipper containing kinase
                     AZK"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase
                     20 isoform 1"
                     /protein_id="NP_001385922.1"
                     /db_xref="GeneID:311743"
                     /db_xref="RGD:1561394"
                     /translation="MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAV
                     KKLLKIEKEAEILSVLSHRNVIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEM
                     DMEHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNH
                     TTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLV
                     VEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTNLPDQCNSF
                     LHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSF
                     EIGAWTEEDVYFWVQQLVGKGESSVEMSGYASLFKENNITGKRLLLLEEEDLKDMGIV
                     SKGHIIHFKSAIEKLTHDYLNLFHFPPLIKDSGEEPEENEEKIVNLELVFGFHLKPGT
                     GPQDCKWKMYMEMDGDEVAIAYIKDVTFNTSLPDAEILKMTKPPFVMEKWIVGIVKDQ
                     TVECTVTYESDVRTPKFTKHVHSIQWDRAKPQDEVKAVQLAIQTLFSSSEGNPGSRSD
                     SSADCQWLDTLRMRQIASHSSLQRSQSNPILGSPFFQYFVNQDSYAAAVRRTQTPVKY
                     QQITPSINPSRSSSPTQYGLTKNFSSLHLNSRDSGFSSLNDSSSERGRYSDRSRNKYY
                     RGSVSLNSSPKGRYGGKSQHSTPSRERYPGKFYRLPQSALNTHQSPDFKRSPNDHDRR
                     APKTIPGMPLHPETAPKAGEEESRVSEGGWTKVEYRKKTHRQLSAKTSKDRTRGNYRG
                     RRNF"
     exon            319..406
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            407..508
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            509..574
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            575..603
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            604..741
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            742..828
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            829..903
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1010
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1011..1146
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1191
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1222
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1362
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1425
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1518
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1635
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..1710
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..1861
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..3237
                     /gene="Map3k20"
                     /gene_synonym="MLTK; RGD1561394; Zak"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtcttcctca ttggcgccgc gcggagaggc ggaatgttca actcctgact ccggcggaag
       61 cgtgggagcc gcgcgggccg ctgtccccga ccccggcctc tgctcgcgcc cggcccggga
      121 gcaaggtttt gtggacgttt actacttggt cattatgaga tgtcgtctct cggtgcctcc
      181 tttgtgcaaa ttaaatttga cgacttgcag ttttttgaaa actgcggtgg aggaagtttc
      241 gggagtgttt atcgagccaa atggatatcc caggacaagg aggtggctgt gaagaagtta
      301 ctcaaaatag agaaagaggc agaaatcctg agtgttctca gtcacaggaa cgtcatccag
      361 ttttacggag tgattctgga acctcccaac tatggcattg tcacagaata tgcatccctg
      421 ggatccctgt atgattacat taacagcaac aggagcgagg aaatggacat ggagcacatc
      481 atgacctggg ccaccgacgt agccaaaggg atgcactacc tgcacatgga ggctccggtc
      541 aaggtgatcc acagagacct caagtcaaga aacgttgtca ttgctgctga cggagtgctg
      601 aagatttgtg acttcggtgc atcgcggttc cataaccata caacacacat gtccttggtt
      661 ggcactttcc catggatggc tccagaagtt atccagagtc tccctgtgtc tgaaacctgt
      721 gacacatatt cctacggtgt ggttctctgg gagatgctaa caagggaggt cccctttaaa
      781 ggtttggaag gattacaagt cgcttggctt gtagtggaaa aaaacgagag attaaccatt
      841 ccaagcagtt gccccagaag ctttgctgaa ctgctacacc agtgttggga ggctgatgcc
      901 aagaaacggc catcgttcaa gcaaatcatt tcaatcctag agtccatgtc aaatgacacg
      961 aatcttcctg accagtgtaa ctcattttta cataacaagg cagaatggag gtgtgaaatt
     1021 gaggctaccc ttgagcggct gaagaagcta gagcgcgatc tcagctttaa agagcaggag
     1081 ctcaaagaaa gggagagacg cttgaagatg tgggagcaga aactgaccga gcagtccaac
     1141 accccgctgc tgccttcctt tgagattggt gcgtggaccg aagaagatgt gtatttttgg
     1201 gttcagcagc tcgtcgggaa aggcgagtcc tcagtcgaga tgagtggata cgcaagcttg
     1261 tttaaggaga acaacatcac aggcaagcgg ttgctgcttc tggaagagga agatctgaaa
     1321 gacatgggca ttgtctccaa gggacacatc attcatttta agtcagcaat tgagaagcta
     1381 acccacgatt acctgaacct gtttcacttc ccgccactaa taaaggattc aggagaggaa
     1441 cctgaagaaa atgaggaaaa aatagtgaac cttgaacttg tttttggatt tcacctgaag
     1501 ccaggcactg gcccacagga ttgcaaatgg aagatgtata tggagatgga tggagacgaa
     1561 gtggctatag catacataaa agacgtgact tttaacacga gcctccctga tgcagagatt
     1621 ttaaagatga caaagccacc gtttgtaatg gagaagtgga tcgtagggat agtgaaggat
     1681 cagacagtgg agtgcaccgt cacctatgag agtgatgtca gaacaccgaa attcactaag
     1741 cacgtgcact caatacagtg ggacagagcg aagcctcagg atgaggtgaa ggcggtccag
     1801 cttgccattc agactctgtt ctccagctca gagggcaacc ctggcagcag gtctgactca
     1861 agtgcggact gccaatggtt agacactctg cgcatgcggc agattgcatc ccactcttct
     1921 ctacagcgtt cccaaagcaa ccccatcctg ggttcccctt tcttccagta ttttgttaac
     1981 caggattcct acgccgctgc agtgaggcgg actcagactc ccgtgaagta ccagcagatt
     2041 acaccaagca tcaacccttc ccggagctcc tccccaacac agtatgggct gaccaaaaac
     2101 ttctcctccc tgcacctcaa ctccagggac agcggcttct ccagcctcaa tgacagctcc
     2161 tccgagaggg gccgctactc agaccgaagc agaaacaagt actaccgcgg tagtgtatcc
     2221 ctcaactctt ctccgaaagg aagatacggt ggaaaaagtc agcattccac accttcaaga
     2281 gaaaggtatc ctggaaagtt ctacaggctg ccccagtcag cgctgaacac tcatcagtcc
     2341 cctgacttca agaggagccc gaatgatcat gaccgtcgtg cgcccaagac catccccggg
     2401 atgcctctgc accccgagac tgctcccaaa gcaggggaag aggagagcag agtgagtgaa
     2461 gggggctgga ctaaagtgga gtatcggaag aagacgcata ggcaactttc ggccaagact
     2521 agcaaggaca gaacccgcgg caactaccgt gggcggagga acttttgaga gattcagtca
     2581 gatgcgcttt tccaagcggg ttatatagtg gtttgtgcaa tggatttttg gtagtaaaat
     2641 gtcttgagat tccagggacc aaaaaagact agtttggggg ttgggaaata cctttttact
     2701 gaaaagacaa ccaaagcatg gccataatta tggctgttag tcgactagag gtggcgatta
     2761 aacttgacaa taactgccct tgtttcatcc cccgaattaa tattgaagcg ggaaaggctg
     2821 tgccattcat tccatgctgg gaacatcttg gggctgggat aaagctagtg agaaccacag
     2881 aaaaatcaaa tttctagatg aagcaggatt cgtctgattt tccctttcat ctaaggatcc
     2941 aagatagctc agcaaagcac tgtgaacaat cctttatttt aaaatgtagg tattttgttc
     3001 atcgtgaatt tttgcatctt attttttatc tactgcgata aaatttcatt tatcttgatt
     3061 gtcgaaggat tttttttttt aaatgcctcc ctaaatgttg tgcccaaggc aagtgcctca
     3121 ctcatctcca ccgagttagt atttatttta gtgagatatt tgtgtttctg tcatggtcag
     3181 ggttgaactg atttggcttg tcatttttag gtaataaaag aagattattg aagttaa
//
