LOCUS       NM_031643               2151 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Rattus norvegicus mitogen activated protein kinase kinase 1
            (Map2k1), mRNA.
ACCESSION   NM_031643
VERSION     NM_031643.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Sun,M.H., Chen,X.C., Han,M., Yang,Y.N., Gao,X.M., Ma,X., Huang,Y.,
            Li,X.M., Gai,M.T., Liu,F., Ma,Y.T. and Chen,B.D.
  TITLE     Cardioprotective effects of constitutively active MEK1 against
            H2O2-induced apoptosis and autophagy in cardiomyocytes via the
            ERK1/2 signaling pathway
  JOURNAL   Biochem Biophys Res Commun 512 (1), 125-130 (2019)
   PUBMED   30876692
  REMARK    GeneRIF: used a constitutively active MEK1 gene (CaMEK)
            transfection strategy to investigate whether CaMEK provides a
            protective effect against apoptosis and autophagy induced by
            hydrogen peroxide (H2O2) in neonatal rat cardiac ventricular
            cardiomyocytes (NCMs) and the underlying mechanisms
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Giguere,H., Dumont,A.A., Berthiaume,J., Oliveira,V., Laberge,G. and
            Auger-Messier,M.
  TITLE     ADAP1 limits neonatal cardiomyocyte hypertrophy by reducing
            integrin cell surface expression
  JOURNAL   Sci Rep 8 (1), 13605 (2018)
   PUBMED   30206251
  REMARK    GeneRIF: Biotin labeling of cell surface proteins from
            cardiomyocytes overexpressing ADAP1 revealed that it reduces the
            surface expression of b1-integrin, an effect that is strongly
            potentiated by Mek1ca overexpression.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2151)
  AUTHORS   Lavoie,H., Sahmi,M., Maisonneuve,P., Marullo,S.A., Thevakumaran,N.,
            Jin,T., Kurinov,I., Sicheri,F. and Therrien,M.
  TITLE     MEK drives BRAF activation through allosteric control of KSR
            proteins
  JOURNAL   Nature 554 (7693), 549-553 (2018)
   PUBMED   29433126
REFERENCE   4  (bases 1 to 2151)
  AUTHORS   Zhang,C., Zhang,B., Wang,H., Tao,Q., Ge,S. and Zhai,Z.
  TITLE     Tumor necrosis factor alpha-stimulated gene-6 (TSG-6) inhibits the
            inflammatory response by inhibiting the activation of P38 and JNK
            signaling pathway and decreases the restenosis of vein grafts in
            rats
  JOURNAL   Heart Vessels 32 (12), 1536-1545 (2017)
   PUBMED   28975447
  REMARK    GeneRIF: TSG-6 can inhibit the inflammatory response of
            transplanted vein grafts in rats and reduce vascular injury by
            downregulation of P38 and JNK signaling pathway.
REFERENCE   5  (bases 1 to 2151)
  AUTHORS   Ciribilli,Y. and Borlak,J.
  TITLE     Oncogenomics of c-Myc transgenic mice reveal novel regulators of
            extracellular signaling, angiogenesis and invasion with clinical
            significance for human lung adenocarcinoma
  JOURNAL   Oncotarget 8 (60), 101808-101831 (2017)
   PUBMED   29254206
  REMARK    Erratum:[Oncotarget. 2018 Dec 14;9(98):37269. doi:
            10.18632/oncotarget.26489.. PMID: 30647859]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2151)
  AUTHORS   Catling,A.D., Schaeffer,H.J., Reuter,C.W., Reddy,G.R. and
            Weber,M.J.
  TITLE     A proline-rich sequence unique to MEK1 and MEK2 is required for raf
            binding and regulates MEK function
  JOURNAL   Mol Cell Biol 15 (10), 5214-5225 (1995)
   PUBMED   7565670
REFERENCE   7  (bases 1 to 2151)
  AUTHORS   Duan,R.D., Zheng,C.F., Guan,K.L. and Williams,J.A.
  TITLE     Activation of MAP kinase kinase (MEK) and Ras by cholecystokinin in
            rat pancreatic acini
  JOURNAL   Am J Physiol 268 (6 Pt 1), G1060-G1065 (1995)
   PUBMED   7611406
REFERENCE   8  (bases 1 to 2151)
  AUTHORS   Suzuki,T., Okumura-Noji,K. and Nishida,E.
  TITLE     ERK2-type mitogen-activated protein kinase (MAPK) and its
            substrates in postsynaptic density fractions from the rat brain
  JOURNAL   Neurosci Res 22 (3), 277-285 (1995)
   PUBMED   7478291
REFERENCE   9  (bases 1 to 2151)
  AUTHORS   Wu,J., Michel,H., Rossomando,A., Haystead,T., Shabanowitz,J.,
            Hunt,D.F. and Sturgill,T.W.
  TITLE     Renaturation and partial peptide sequencing of mitogen-activated
            protein kinase (MAP kinase) activator from rabbit skeletal muscle
  JOURNAL   Biochem J 285 (Pt 3) (Pt 3), 701-705 (1992)
   PUBMED   1379797
REFERENCE   10 (bases 1 to 2151)
  AUTHORS   Williams,A.F., Barclay,A.N., Letarte-Muirhead,M. and Morris,R.J.
  TITLE     Rat thy-1 antigens from thymus and brain: their tissue
            distribution, purification, and chemical composition
  JOURNAL   Cold Spring Harb Symp Quant Biol 41 Pt 1, 51-61 (1977)
   PUBMED   70317
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AI603089.1 and BC089772.1.
            
            On Mar 23, 2010 this sequence version replaced NM_031643.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC089772.1, D13341.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN16676785 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 AI603089.1         1-6
            7-2151              BC089772.1         1-2145
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q24"
     gene            1..2151
                     /gene="Map2k1"
                     /gene_synonym="Mek1"
                     /note="mitogen activated protein kinase kinase 1"
                     /db_xref="GeneID:170851"
                     /db_xref="RGD:70495"
     exon            1..127
                     /gene="Map2k1"
                     /gene_synonym="Mek1"
                     /inference="alignment:Splign:2.1.0"
     CDS             48..1229
                     /gene="Map2k1"
                     /gene_synonym="Mek1"
                     /EC_number="2.7.12.2"
                     /note="MAPKK 1; MAPK/ERK kinase 1; MAP kinase kinase 1;
                     ERK activator kinase 1; MAP kinase/Erk kinase 1"
                     /codon_start=1
                     /product="dual specificity mitogen-activated protein
                     kinase kinase 1"
                     /protein_id="NP_113831.1"
                     /db_xref="GeneID:170851"
                     /db_xref="RGD:70495"
                     /translation="MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDE
                     QQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLE
                     IKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGR
                     IPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDS
                     MANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELLFG
                     CQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEF
                     QDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAS
                     I"
     exon            128..338
                     /gene="Map2k1"
                     /gene_synonym="Mek1"
                     /inference="alignment:Splign:2.1.0"
     exon            339..485
                     /gene="Map2k1"
                     /gene_synonym="Mek1"
                     /inference="alignment:Splign:2.1.0"
     exon            486..563
                     /gene="Map2k1"
                     /gene_synonym="Mek1"
                     /inference="alignment:Splign:2.1.0"
     exon            564..615
                     /gene="Map2k1"
                     /gene_synonym="Mek1"
                     /inference="alignment:Splign:2.1.0"
     exon            616..740
                     /gene="Map2k1"
                     /gene_synonym="Mek1"
                     /inference="alignment:Splign:2.1.0"
     exon            741..942
                     /gene="Map2k1"
                     /gene_synonym="Mek1"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1007
                     /gene="Map2k1"
                     /gene_synonym="Mek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1069
                     /gene="Map2k1"
                     /gene_synonym="Mek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1115
                     /gene="Map2k1"
                     /gene_synonym="Mek1"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..2131
                     /gene="Map2k1"
                     /gene_synonym="Mek1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggccgcgcg ctccctgctg agttgcaggc tgtttcccgg ctgcaagatg cccaagaaga
       61 agccgacgcc catccagctg aacccggccc ccgatggctc cgcggttaac gggaccagct
      121 cggccgagac caacctggag gccttgcaga agaagctgga ggagctggag ctggacgagc
      181 agcagcggaa gcgccttgag gcctttctga cgcagaagca gaaggtggga gagttgaagg
      241 atgatgactt tgagaagatc agtgaactgg gggctggcaa tggtggagtg gtgttcaagg
      301 tctcccacaa gccatctggc ctggttatgg ctaggaagct aattcacctg gagatcaaac
      361 ccgcaatccg gaaccagatc atccgggagc tgcaggtgct gcatgagtgc aactccccgt
      421 acatagtggg cttctacggg gccttctaca gtgacggcga gatcagcatc tgcatggagc
      481 acatggatgg tgggtccttg gatcaagtgc tgaagaaagc tggaagaatt cctgagcaaa
      541 ttttaggaaa agtcagcatc gctgtgataa aaggcctgac atatctacga gagaagcaca
      601 agattatgca cagagatgtc aagccttcca acattctagt gaactcacgt ggggagatca
      661 aactctgcga ttttggggtc agcgggcagc taattgactc catggccaac tccttcgtgg
      721 gaacaaggtc ctacatgtcg cctgagagac tccaggggac tcactactct gtgcagtcgg
      781 acatctggag catggggctc tctctggtgg agatggcagt tggaagatac cccattcctc
      841 ctcctgatgc caaggagctg gagctgctgt ttggatgcca ggtggaagga gacgcggccg
      901 aaacgccacc caggccaagg acccctggga ggcccctcag ctcatatgga atggatagcc
      961 gacctcccat ggcaattttt gagttgttgg attacatcgt caatgagcct cctccaaaac
     1021 tgcccagtgg agtattcagt ctggaatttc aggattttgt gaataagtgc ttaataaaga
     1081 accctgcaga gagagcagat ctgaagcagc tcatggtaca tgctttcatc aagagatctg
     1141 atgccgagga ggtagacttc gcaggctggc tctgctccac cattgggctt aaccagccca
     1201 gcacaccaac ccacgctgcc agcatctgag cctttgggaa gcagcagaga ggaatcctct
     1261 gcccagtggc atgccatgtt gctttcaggc ctctcccatg cttgtctatg ttcagatgtg
     1321 catctcatct gtgacaaagg atgaagaaca cagcatgtgc caaatcgtac ttgtgtcatt
     1381 tttaatattg tctttatcgc tatggttact cccctaagtg gattggcttt gtgcttgggg
     1441 ctatttgtct gttcatcaaa tacatgccag gttgaactac agtgaaaccc tggtgacctg
     1501 ggtggtcttc ttactgatgt ttgcgctgct gttcatcgtg actcactagc tggctgcctg
     1561 tattgtcagg attctcggac ccttggtact tcactcttgc tggtgacctc tcagtctgag
     1621 gagagggggc cttctgagac ccttcacagg cagtgcatgc atgaaaagca tgctttgctg
     1681 ctactgaaat gagcaccaga acgtgtacat catggtattt tatttttgct tttggtatag
     1741 aactcagcag ttcccattta aaaaaaaaat ctaaccagag cccatcactg ccatgatagc
     1801 tggggcttca gtctgtctac tgtggtgatt tttagacttc tggttgtatt tctatattta
     1861 tttttaaata tactgtgtgg gatacttagt ggtatatgtc tctgagtttg gattagtgtt
     1921 tctaaattgg tagttatttt gaatgtcaca aatggattaa ggaatcaacg tatcaagagt
     1981 tctatctttc ttccagtcta agtaccaatg ctattgtaaa cgtgtatagt gcctacaaat
     2041 tgtatgaaaa cccttttaac cactttactc aagatgttta tcaaatctaa tctcttattc
     2101 taataaaaat actatcaagt taaagtaaaa aaaaaaaaaa aaaaaaaaaa a
//
