LOCUS       NM_199404               3150 bp    mRNA    linear   ROD 23-MAR-2023
DEFINITION  Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1),
            mRNA.
ACCESSION   NM_199404 XM_341658
VERSION     NM_199404.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Lu H, Ma F, Wang H, Geng PS, Wang SS, Wang JG, Wu CC and Zhao BY.
  TITLE     The effects of swainsonine on the activity and expression of
            alpha-mannosidase in BRL-3A cells
  JOURNAL   Toxicon 99, 44-50 (2015)
   PUBMED   25797317
  REMARK    GeneRIF: Data suggest that hepatocyte expression of Man2a1 (Golgi
            apparatus alpha-mannosidase) and Man2b1 (lysosome
            alpha-mannosidase) is down-regulated by biological toxin
            swainsonine as part of locoweed/Oxytropis kansuensis hepatotoxic
            damage.
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Thaysen-Andersen M, Venkatakrishnan V, Loke I, Laurini C, Diestel
            S, Parker BL and Packer NH.
  TITLE     Human neutrophils secrete bioactive paucimannosidic proteins from
            azurophilic granules into pathogen-infected sputum
  JOURNAL   J Biol Chem 290 (14), 8789-8802 (2015)
   PUBMED   25645918
REFERENCE   3  (bases 1 to 3150)
  AUTHORS   D'Hooge R, Lullmann-Rauch R, Beckers T, Balschun D, Schwake M,
            Reiss K, von Figura K and Saftig P.
  TITLE     Neurocognitive and psychotiform behavioral alterations and enhanced
            hippocampal long-term potentiation in transgenic mice displaying
            neuropathological features of human alpha-mannosidosis
  JOURNAL   J Neurosci 25 (28), 6539-6549 (2005)
   PUBMED   16014715
REFERENCE   4  (bases 1 to 3150)
  AUTHORS   Stinchi S, Lullmann-Rauch R, Hartmann D, Coenen R, Beccari T,
            Orlacchio A, von Figura K and Saftig P.
  TITLE     Targeted disruption of the lysosomal alpha-mannosidase gene results
            in mice resembling a mild form of human alpha-mannosidosis
  JOURNAL   Hum Mol Genet 8 (8), 1365-1372 (1999)
   PUBMED   10400983
REFERENCE   5  (bases 1 to 3150)
  AUTHORS   Beccari T, Appolloni MG, Costanzi E, Stinchi S, Stirling JL, Della
            Fazia MA, Servillo G, Viola MP and Orlacchio A.
  TITLE     Lysosomal alpha-mannosidases of mouse tissues: characteristics of
            the isoenzymes, and cloning and expression of a full-length cDNA
  JOURNAL   Biochem J 327 (Pt 1) (Pt 1), 45-49 (1997)
   PUBMED   9355733
REFERENCE   6  (bases 1 to 3150)
  AUTHORS   Merkle RK, Zhang Y, Ruest PJ, Lal A, Liao YF and Moremen KW.
  TITLE     Cloning, expression, purification, and characterization of the
            murine lysosomal acid alpha-mannosidase
  JOURNAL   Biochim Biophys Acta 1336 (2), 132-146 (1997)
   PUBMED   9305783
REFERENCE   7  (bases 1 to 3150)
  AUTHORS   Liao YF, Lal A and Moremen KW.
  TITLE     Cloning, expression, purification, and characterization of the
            human broad specificity lysosomal acid alpha-mannosidase
  JOURNAL   J Biol Chem 271 (45), 28348-28358 (1996)
   PUBMED   8910458
REFERENCE   8  (bases 1 to 3150)
  AUTHORS   Sleat DE, Sohar I, Lackland H, Majercak J and Lobel P.
  TITLE     Rat brain contains high levels of mannose-6-phosphorylated
            glycoproteins including lysosomal enzymes and palmitoyl-protein
            thioesterase, an enzyme implicated in infantile neuronal
            lipofuscinosis
  JOURNAL   J Biol Chem 271 (32), 19191-19198 (1996)
   PUBMED   8702598
REFERENCE   9  (bases 1 to 3150)
  AUTHORS   Rajashree S and Puvanakrishnan R.
  TITLE     Alterations in certain lysosomal glycohydrolases and cathepsins in
            rats on dexamethasone administration
  JOURNAL   Mol Cell Biochem 154 (2), 165-170 (1996)
   PUBMED   8717430
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC061819.1.
            
            On Jan 12, 2004 this sequence version replaced XM_341658.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC061819.1, SRR8487230.54868.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760494
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q11"
     gene            1..3150
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /note="mannosidase, alpha, class 2B, member 1"
                     /db_xref="GeneID:361378"
                     /db_xref="RGD:3039"
     exon            1..184
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..3055
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /EC_number="3.2.1.24"
                     /note="mannosidase, alpha B, lysosomal; mannosidase 2,
                     alpha B1; lysosomal alpha-mannosidase"
                     /codon_start=1
                     /product="lysosomal alpha-mannosidase precursor"
                     /protein_id="NP_955436.1"
                     /db_xref="GeneID:361378"
                     /db_xref="RGD:3039"
                     /translation="MGTGSLAPGVRAGGGNTGWLWMSSCNLGLPVLSISFLIWLLLAA
                     PGAQAAGYKTCPTTKPGMLNVHLLPHTHDDVGWLKTVDQYYYGIMSDVQHASVQYILD
                     SVIYSLLNDPTRRFIYVEMAFFSRWWKQQTNVTQDAVRNLVRQGRLEFVNGGWVMNDE
                     AATHYGAIVDQMTLGLRFLQDTFGSDGLPRVAWHIDPFGHSREQASLFAQMGFDGFFL
                     GRIDYQDKFNRKRKLKMEELWRASASLKPPAADLFTGVLPNNYNPPKDLCWDVLCTDP
                     PVVDDPTSPEFNANKLVDYFLNLASSQKKYYRTNHTVMTMGSDFQYENANMWFKNMDK
                     LIRLVNEQQANGSKVHVLYSTPSCYLWELNKANLTWTVKEDDFFPYADGPHMFWTGYF
                     SSRPALKRYERLSYNFLQVCNQLEALVGPEAKVGPYGSGDSAPLNEAMAVLQHHDAVT
                     GTARQNVVNDYAKQLAAGWGPCEVLVSNALARLSLYKQNFSFCREINISICPASQTSE
                     HFRVIIYNPVGRKVDLMVRLPVSEGIFLVKDPNDRRISSNVVMVPSAYSKTYQWELLF
                     PASVPALGFSIYSVNKMSGHNHQAHNLTARPKKSKSRVLVIENKYIRATFDSDTGLLR
                     KIENLEQNISLPVRQGFFWYNASAGDEESPQASGAYIFRPSHRKPLPVSHWAQVTLVK
                     TNLVQEVHQNFSAWCSQVIRLYEGQRHLELEWTVGPIPVKDDWGKEVISRFNTPMRTR
                     GQFFTDSNGREILKRRDDFRPTWTLNQTEPVAGNYYPVNTRIYITDGHMQLTVLTDRS
                     QGGSSLLDGSLELMVHRRLLVDDERGVAEPLLETDTGDKVRGRHLVILSSVSDAAARH
                     RLLAEQEVLAPQVVLAHGGSSPYHSQAPKMQFSALRRELPPQVHLLTLARWGPKMLLL
                     RLEHQFAVKEDSNRNLSSPVTLNLQNLFKTFTINYLQETTLAANQPLSRVSRLKWMTD
                     TGPISYPAPSRLDPTSITLQPMQIRTFLASVQWQRLI"
     exon            185..287
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            288..461
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            462..655
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            656..788
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            789..934
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1051
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1134
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1255
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1334
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            1335..1444
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1552
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1669
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            1670..1855
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            1856..1953
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            1954..2071
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            2072..2190
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            2191..2292
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            2293..2380
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            2381..2461
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            2462..2686
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            2687..2842
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            2843..2945
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
     exon            2946..3138
                     /gene="Man2b1"
                     /gene_synonym="Manb"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctgcggaagc agcgtcgccg gaaccatggg caccggatcg cttgcccctg gtgtccgtgc
       61 tggtggcggg aacactgggt ggctctggat gagctcctgc aacttaggtt tgccagtcct
      121 gtctatttct tttcttattt ggttgttact ggctgcgcct ggcgctcagg ccgcgggtta
      181 caagacatgt cccaccacga agccaggcat gctcaacgtg cacctgctgc ctcacacaca
      241 cgatgatgtg ggctggctga agacagtaga ccaatactac tacggcatca tgagtgacgt
      301 ccagcatgca tctgttcagt acatcctaga ctcagtgatt tactctctac tgaacgatcc
      361 cacccgtcgc ttcatatacg tggaaatggc tttcttctcc cgttggtgga agcagcagac
      421 caatgtaaca caggacgctg tacggaacct ggtgcgccag gggcgcctgg agtttgtcaa
      481 tggtggctgg gtaatgaatg atgaggcagc cactcactat ggtgctattg tggaccagat
      541 gacacttggg ctacgcttcc tgcaggacac atttggtagt gatggacttc cccgtgtggc
      601 ctggcacatt gacccttttg gccattctcg agaacaggcc tccctgtttg cccagatggg
      661 ttttgatggc ttcttcctcg ggcgcattga ctaccaagat aaatttaacc ggaagagaaa
      721 gctgaagatg gaggaactct ggagggctag cgccagcttg aagccccctg ctgctgacct
      781 ctttactggt gtgctcccca ataattacaa ccctccaaaa gacctgtgct gggatgtgct
      841 gtgtacagac cctcccgtgg tggacgatcc cacaagccct gagtttaatg ccaataagtt
      901 ggttgattac ttcctgaacc tcgcctcttc ccagaaaaag tattaccgaa ccaaccacac
      961 cgtgatgacc atgggctcag acttccagta tgagaatgcc aacatgtggt tcaaaaacat
     1021 ggacaagctt atccgcctgg tcaacgagca gcaggcaaac gggagcaagg tccacgtgct
     1081 ctactccacc ccttcgtgtt acctctggga gctgaacaag gccaatctca cttggacagt
     1141 gaaggaggac gacttctttc cttatgctga tggcccccac atgttctgga ctggctattt
     1201 ctccagtagg ccagccctca agcgctatga gcgcctcagc tacaacttcc tgcaggtgtg
     1261 caaccagctg gaagcactgg tgggtcccga ggccaaagtg ggaccctacg gctcaggaga
     1321 cagtgcaccc ctcaatgagg caatggcggt gctgcagcac cacgatgctg tcactggtac
     1381 tgcaaggcag aatgttgtca atgactatgc gaagcagctg gcggcaggct ggggaccctg
     1441 tgaggttctc gtgagcaacg ctctggcgcg actcagcctt tacaaacaga acttctcatt
     1501 ctgtcgggag atcaacatca gtatctgccc agccagccag acgtcagagc acttccgggt
     1561 aattatttac aaccccgtgg ggcggaaggt ggatctgatg gttcggctgc cggtcagtga
     1621 aggcatcttc cttgtgaagg accctaacga taggagaatt tccagtaatg tggtgatggt
     1681 tcccagtgct tacagtaaga cgtatcaatg ggagctgctg ttcccagcct cagtgcctgc
     1741 cctgggcttc agcatctact ctgtgaacaa aatgagtggc cataaccatc aggcccacaa
     1801 cctaacggcc aggcccaaga aatccaagtc ccgtgtctta gtcattgaaa ataagtacat
     1861 acgggccaca tttgactctg acacagggtt attgaggaag attgaaaacc tggaacagaa
     1921 catttcgctc cctgtgagac aaggcttctt ttggtacaat gccagcgctg gtgatgagga
     1981 gagtcctcag gcctctggtg cctacatctt cagacccagt catcgtaaac cactgcctgt
     2041 gagccactgg gctcaggtca ctttggtgaa gacaaacttg gtgcaggagg tacaccagaa
     2101 tttttcagcc tggtgttccc aagtgattcg cctgtatgaa ggacagcgtc atttggagct
     2161 ggagtggacg gtgggaccaa tcccagtgaa agatgactgg ggaaaggaag tcatcagccg
     2221 ctttaacacc ccgatgagaa ccagggggca gttcttcacg gacagcaacg gccgggaaat
     2281 cctgaagagg agggacgatt tccgacccac ctggacatta aatcagactg aaccagtggc
     2341 tggaaattac taccctgtca acactcgcat ttacatcacg gacgggcata tgcagctgac
     2401 cgtgctgacc gaccgttccc aggggggcag cagtctgctg gatggctcat tggagctcat
     2461 ggttcaccga cggctgttgg tagatgatga acgtggggtg gcggaaccct tgctggagac
     2521 agatacgggg gataaggttc gagggcgcca ccttgtgatt ttgagttctg tcagtgatgc
     2581 ggctgcaaga caccgactgc tggcggagca ggaagtcctg gcccctcagg tggtgctggc
     2641 ccacgggggt agcagtccct atcactcgca ggcaccaaaa atgcagttct ccgcacttcg
     2701 ccgggagcta cctccgcagg tgcatctgct caccctggct cgctggggtc caaagatgct
     2761 gctgctgcgc ttggagcacc agttcgctgt gaaggaagat tcaaatcgca acctgagctc
     2821 tccggtgacc ttgaacttgc agaacctgtt caagaccttc actatcaact acctgcagga
     2881 gaccacactg gcagccaacc agcccctatc cagggtttcc aggctcaagt ggatgacaga
     2941 tactggtccc atctcctacc ctgctccttc ccggttggac cctacctcaa tcacactgca
     3001 gcctatgcaa atccgtacct tcctggcctc ggttcaatgg cagagactca tctaggcctg
     3061 ccaggaggag gggcaggcct ccccttctct tgctggaacc actcacaaaa atcattaaaa
     3121 gacttctatt gagactcaaa aaaaaaaaaa
//
