LOCUS       NP_620784               1470 aa            linear   ROD 30-MAR-2024
DEFINITION  membrane-associated guanylate kinase, WW and PDZ domain-containing
            protein 3 [Rattus norvegicus].
ACCESSION   NP_620784 XP_001068223
VERSION     NP_620784.3
DBSOURCE    REFSEQ: accession NM_139084.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 1470)
  AUTHORS   Xu,M. and Yu,T.
  TITLE     MiR-20b-5p contributes to the dysfunction of vascular smooth muscle
            cells by targeting MAGI3 in hypertension
  JOURNAL   J Mol Histol 53 (2), 187-197 (2022)
   PUBMED   34985721
  REMARK    GeneRIF: MiR-20b-5p contributes to the dysfunction of vascular
            smooth muscle cells by targeting MAGI3 in hypertension.
REFERENCE   2  (residues 1 to 1470)
  AUTHORS   He,J., Bellini,M., Inuzuka,H., Xu,J., Xiong,Y., Yang,X.,
            Castleberry,A.M. and Hall,R.A.
  TITLE     Proteomic analysis of beta1-adrenergic receptor interactions with
            PDZ scaffold proteins
  JOURNAL   J Biol Chem 281 (5), 2820-2827 (2006)
   PUBMED   16316992
REFERENCE   3  (residues 1 to 1470)
  AUTHORS   Valiente,M., Andres-Pons,A., Gomar,B., Torres,J., Gil,A.,
            Tapparel,C., Antonarakis,S.E. and Pulido,R.
  TITLE     Binding of PTEN to specific PDZ domains contributes to PTEN protein
            stability and phosphorylation by microtubule-associated
            serine/threonine kinases
  JOURNAL   J Biol Chem 280 (32), 28936-28943 (2005)
   PUBMED   15951562
REFERENCE   4  (residues 1 to 1470)
  AUTHORS   Hinojos,C.A., Boerwinkle,E., Fornage,M. and Doris,P.A.
  TITLE     Combined genealogical, mapping, and expression approaches to
            identify spontaneously hypertensive rat hypertension candidate
            genes
  JOURNAL   Hypertension 45 (4), 698-704 (2005)
   PUBMED   15710778
REFERENCE   5  (residues 1 to 1470)
  AUTHORS   Meyer,G., Varoqueaux,F., Neeb,A., Oschlies,M. and Brose,N.
  TITLE     The complexity of PDZ domain-mediated interactions at glutamatergic
            synapses: a case study on neuroligin
  JOURNAL   Neuropharmacology 47 (5), 724-733 (2004)
   PUBMED   15458844
REFERENCE   6  (residues 1 to 1470)
  AUTHORS   Yao,R., Natsume,Y. and Noda,T.
  TITLE     MAGI-3 is involved in the regulation of the JNK signaling pathway
            as a scaffold protein for frizzled and Ltap
  JOURNAL   Oncogene 23 (36), 6023-6030 (2004)
   PUBMED   15195140
REFERENCE   7  (residues 1 to 1470)
  AUTHORS   Adamsky,K., Arnold,K., Sabanay,H. and Peles,E.
  TITLE     Junctional protein MAGI-3 interacts with receptor tyrosine
            phosphatase beta (RPTP beta) and tyrosine-phosphorylated proteins
  JOURNAL   J Cell Sci 116 (Pt 7), 1279-1289 (2003)
   PUBMED   12615970
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On Nov 27, 2020 this sequence version replaced NP_620784.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF255614.1, SRR26643288.11040.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q34"
     Protein         1..1470
                     /product="membrane-associated guanylate kinase, WW and PDZ
                     domain-containing protein 3"
                     /note="MAGI-3; scaffolding-like protein;
                     membrane-associated guanylate kinase inverted 3;
                     scaffolding protein SLIPR; membrane-associated guanylate
                     kinase-related (MAGI-3)"
                     /calculated_mol_wt=160426
     Region          14..106
                     /region_name="PDZ0_MAGI-1_3-like"
                     /note="PDZ domain 0 of membrane-associated guanylate
                     kinase inverted 1 (MAGI-1), MAGI-2, and MAGI-3, and
                     related domains; cd06730"
                     /db_xref="CDD:467212"
     Region          18..108
                     /region_name="Interaction with ADRB1 and TGFA.
                     /evidence=ECO:0000269|PubMed:16316992"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JK71.2)"
     Site            order(28..34,46,49,86,90,93..94)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467212"
     Region          125..>199
                     /region_name="NK"
                     /note="Nucleoside/nucleotide kinase (NK) is a protein
                     superfamily consisting of multiple families of enzymes
                     that share structural similarity and are functionally
                     related to the catalysis of the reversible phosphate group
                     transfer from nucleoside triphosphates...; cl17190"
                     /db_xref="CDD:450170"
     Region          184..266
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JK71.2)"
     Region          <211..>373
                     /region_name="HUL4"
                     /note="Ubiquitin-protein ligase [Posttranslational
                     modification, protein turnover, chaperones]; COG5021"
                     /db_xref="CDD:227354"
     Site            236
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9EQJ9; propagated from
                     UniProtKB/Swiss-Prot (Q9JK71.2)"
     Region          299..329
                     /region_name="WW"
                     /note="Two conserved tryptophans domain; also known as the
                     WWP or rsp5 domain; around 40 amino acids; functions as an
                     interaction module in a diverse set of signalling
                     proteins; binds specific proline-rich sequences but at low
                     affinities compared to other...; cd00201"
                     /db_xref="CDD:238122"
     Site            order(313,324)
                     /site_type="active"
                     /note="binding pocket [active]"
                     /db_xref="CDD:238122"
     Region          345..375
                     /region_name="WW"
                     /note="Two conserved tryptophans domain; also known as the
                     WWP or rsp5 domain; around 40 amino acids; functions as an
                     interaction module in a diverse set of signalling
                     proteins; binds specific proline-rich sequences but at low
                     affinities compared to other...; cd00201"
                     /db_xref="CDD:238122"
     Site            order(359,370)
                     /site_type="active"
                     /note="binding pocket [active]"
                     /db_xref="CDD:238122"
     Region          411..495
                     /region_name="PDZ1_MAGI-1_3-like"
                     /note="PDZ domain 1 of membrane-associated guanylate
                     kinase inverted 1 (MAGI-1), MAGI-2, and MAGI-3, and
                     related domains; cd06731"
                     /db_xref="CDD:467213"
     Site            order(411,432..436,438,459,461,467,469..470,492,494..495)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467213"
     Region          413..495
                     /region_name="Interaction with PTEN.
                     /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JK71.2)"
     Site            order(422..428,440,443,473,477,480..481)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467213"
     Region          550..575
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JK71.2)"
     Region          577..658
                     /region_name="PDZ2_MAGI-1_3-like"
                     /note="PDZ domain 2 of membrane-associated guanylate
                     kinase inverted 1 (MAGI-1), MAGI-2, and MAGI-3, and
                     related domains; cd06732"
                     /db_xref="CDD:467214"
     Site            order(590..596,605,608,635,639,642..643)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467214"
     Site            598
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q5TCQ9; propagated from
                     UniProtKB/Swiss-Prot (Q9JK71.2)"
     Region          665..700
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JK71.2)"
     Site            702
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JK71.2)"
     Region          727..811
                     /region_name="PDZ3_MAGI-1_3-like"
                     /note="PDZ domain 3 of membrane-associated guanylate
                     kinase inverted 1 (MAGI-1), MAGI-2, and MAGI-3, and
                     related domains; cd06733"
                     /db_xref="CDD:467215"
     Region          729..811
                     /region_name="Interaction with ADGRB1.
                     /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JK71.2)"
     Site            order(738..744,756,759,789,793,796..797)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467215"
     Region          818..847
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JK71.2)"
     Site            833
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JK71.2)"
     Region          851..936
                     /region_name="PDZ4_MAGI-1_3-like"
                     /note="PDZ domain 4 of membrane-associated guanylate
                     kinase inverted 1 (MAGI-1), MAGI-2, and MAGI-3, and
                     related domains; cd06734"
                     /db_xref="CDD:467216"
     Region          852..939
                     /region_name="Interaction with LPAR2 and GRIN2B.
                     /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JK71.2)"
     Site            order(862..868,884,887,917,921,924..925)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467216"
     Site            916
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9EQJ9; propagated from
                     UniProtKB/Swiss-Prot (Q9JK71.2)"
     Region          939..976
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JK71.2)"
     Region          1020..1102
                     /region_name="PDZ5_MAGI-1_3-like"
                     /note="PDZ domain 5 of membrane-associated guanylate
                     kinase inverted 1 (MAGI-1), MAGI-2, and MAGI-3, and
                     related domains; cd06735"
                     /db_xref="CDD:467217"
     Site            order(1031..1037,1049,1052,1082,1086,1089..1090)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467217"
     Region          1115..>1405
                     /region_name="PTZ00112"
                     /note="origin recognition complex 1 protein; Provisional"
                     /db_xref="CDD:240274"
     Region          1124..1146
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JK71.2)"
     Region          1167..1470
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JK71.2)"
     Site            1321
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JK71.2)"
     CDS             1..1470
                     /gene="Magi3"
                     /gene_synonym="Slipr"
                     /coded_by="NM_139084.3:128..4540"
                     /db_xref="GeneID:245903"
                     /db_xref="RGD:621362"
ORIGIN      
        1 msktlkkkkh wlskvqecav swagppgdlg aeirggaerg efpylgrlrd epgggggtcc
       61 vvsgkapspg dvllevngtp vsgltnrdtl avirhfrepi rlktvkpgkv inkdlrhyls
      121 lqfqkgsidh klqqvirdnl ylrtipcttr aprdgevpgv dynfisveqf kaleesgall
      181 esgtydgnfy gtpkppaeps pfqpdpvdqv lfdnefdtes qrkrttsvsk mermdsslpe
      241 eeededkeav ngsgsmetre mhsessdcwm ktvpsynqtn rsmdfrnymm rdenleplpk
      301 nwemaytdtg tiyfidhntk tttwldprlc kkakapedce dgelpygwek iedpqygtyy
      361 vdhlnqktqf enpveeakrk kqigqaeths aktdverahf trdpsqlkgv lvraslkkst
      421 mgfgftiigg drpdeflqvk nvlkdgpaaq dgkmapgdvi vdingncvlg hthadvvqmf
      481 qlvpvnqyvn ltlcrgyalp ddsedpvvdi vaatpvingq slakgeacms tqdfklgamv
      541 ldqngksgkl lssdhlngps dsneqrasla ssgssqpelv tiplvkgpkg fgfaiadspt
      601 gqkvkmilds qwcqglqkgd iikeiyhqnv qnlthlqvve vlkqfpvgad vpllilrggp
      661 csptktakmk tdtketsgsl etineptpqp mpfppsiirs gspkldpsev ylksktlyed
      721 kppntkdldv flrkqesgfg frvlggdgpd qsiyigaiip lgaaekdgrl raadelmcid
      781 gipvkgkshk qvldlmttaa rnghvlltvr rkifygekqp edespqafsq sgsprlnrte
      841 lptrsapqes ydvilqrken egfgfvilts kskpppgvip hkigrvidgs padrcgrlkv
      901 gdhisavngq sivdlshdni vqlikdagvt vtltvvaeee hhgppsgtns arqspalqhr
      961 pmgqaqathi pgdrtalege vgkdvcssyr hswsdhkhla qpdtavisvv gsrhsqslgc
     1021 ypvelergpr gfgfslrggk eynmglfilr laedgpaikd grihvgdqiv eingeptqgi
     1081 thtraieliq aggnkvllll rpgtglipdh gdwdiyspss snviydeqpp plpsshsaat
     1141 feeshvpvte dslirvqtce kaeelkdtvq ekkstlngsq pemkyqsvqk nvskkdpsrs
     1201 hghgdknlpk gengvtrrgr saspkksvnr hseehlekip rplrsdpkgk srdrslsprk
     1261 genkgqvtik agsgqdpcrk drgrsssprk qqkiggnsls ntegklseag srraaglssd
     1321 speqlpegke ksgvsrkdlk lsqlgknrtr spekrsskvd easlpskkts dtasrvvsek
     1381 ekgrkpgtge rsrdktgesv qtsakpltqe agekmalska sevtdrgker aggapesssp
     1441 vkkapitpgp wrvprankvt gtagmadkql
//
