LOCUS       NM_001034139            2997 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus leucine rich repeat containing 8 VRAC subunit E
            (Lrrc8e), mRNA.
ACCESSION   NM_001034139 XM_221814
VERSION     NM_001034139.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Lutter,D., Ullrich,F., Lueck,J.C., Kempa,S. and Jentsch,T.J.
  TITLE     Selective transport of neurotransmitters and modulators by distinct
            volume-regulated LRRC8 anion channels
  JOURNAL   J Cell Sci 130 (6), 1122-1133 (2017)
   PUBMED   28193731
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Syeda,R., Qiu,Z., Dubin,A.E., Murthy,S.E., Florendo,M.N.,
            Mason,D.E., Mathur,J., Cahalan,S.M., Peters,E.C., Montal,M. and
            Patapoutian,A.
  TITLE     LRRC8 Proteins Form Volume-Regulated Anion Channels that Sense
            Ionic Strength
  JOURNAL   Cell 164 (3), 499-511 (2016)
   PUBMED   26824658
REFERENCE   3  (bases 1 to 2997)
  AUTHORS   Voss,F.K., Ullrich,F., Munch,J., Lazarow,K., Lutter,D., Mah,N.,
            Andrade-Navarro,M.A., von Kries,J.P., Stauber,T. and Jentsch,T.J.
  TITLE     Identification of LRRC8 heteromers as an essential component of the
            volume-regulated anion channel VRAC
  JOURNAL   Science 344 (6184), 634-638 (2014)
   PUBMED   24790029
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000012.1.
            
            On Nov 27, 2020 this sequence version replaced NM_001034139.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC105779.1, DN948377.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN16093042, SAMN16093045
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                JAXUCZ010000012.1  7376147-7376213
            68-210              JAXUCZ010000012.1  7380972-7381114
            211-2997            JAXUCZ010000012.1  7384456-7387242
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12p12"
     gene            1..2997
                     /gene="Lrrc8e"
                     /gene_synonym="RGD1311979"
                     /note="leucine rich repeat containing 8 VRAC subunit E"
                     /db_xref="GeneID:304203"
                     /db_xref="RGD:1311979"
     exon            1..67
                     /gene="Lrrc8e"
                     /gene_synonym="RGD1311979"
                     /inference="alignment:Splign:2.1.0"
     exon            68..210
                     /gene="Lrrc8e"
                     /gene_synonym="RGD1311979"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..2460
                     /gene="Lrrc8e"
                     /gene_synonym="RGD1311979"
                     /note="leucine-rich repeat-containing protein 8E; leucine
                     rich repeat containing 8 family, member E"
                     /codon_start=1
                     /product="volume-regulated anion channel subunit LRRC8E"
                     /protein_id="NP_001029311.1"
                     /db_xref="GeneID:304203"
                     /db_xref="RGD:1311979"
                     /translation="MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCT
                     LQVTQDKIICLPSHESRENSSEAPCQQLLPQGISEQIGGLRELSGLKNNLDLQQYSFI
                     NQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSP
                     WTTRALSEVSGENHKGPAAGRATVTTVTTVGTGTGKVGEGEKEKVLIEPEKVVTEPPA
                     VTLLDKKEGEQAKALFEKVKKFRVHVEEGDILYSMYIRQTVLKVCKFFAILVYNLVYV
                     EKISFLVACRVETSEITGYASFCCNHTKAHLFSKLAFCYISFVCVYGITCLYTLYWLF
                     HRPLKEYSFRSVREETGMNDIPDVRNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRL
                     KQLNLNHEWTPDKLRQKLQRNARGRLELSLCMLPGLPDTVFELSEVEALRLEAICDIS
                     FPPGLSQLVNLQELSLLHSPARLPFSSQIFLRDRLKVICVKFEELREVPLWVFGLRGL
                     EELHLEGLFPPEMARGATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHLQRLSLHN
                     DGARLLALNSLKKLAVLRELELVACGLERIPHAIFSLGALQELDLKDNHLRSIEEILS
                     FQHCRKLVILRLWHNQIAYVPEHVRKLRSLEQLYLSHNKLETLPAQLGQCFGLRLLDV
                     SHNGLRSLPPELGLLQSLQHLALSYNALESLPDELFFCHKLRTLLLGYNHLTQFSPHV
                     AALQALSRLELKGNRLEALPEELGDCKGLKKSGLLVEDTLYEGLPAEVREKMEEE"
     exon            211..2997
                     /gene="Lrrc8e"
                     /gene_synonym="RGD1311979"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgggagtcc gggtccatct actcccagtg atctaggagt tgggccccag agttccatcc
       61 cagagcaaca ggatgatccc agtggcggag ttcaagcagt tcacggagca gcagcctgcg
      121 ttcaaggtgc tcaaaccctg gtgggatgtt ttggctgagt acctcactgt ggctatgctc
      181 atgattgggg tgttcgggtg caccctccag gtgacacagg acaagatcat ctgcctgccc
      241 agccatgaat ctcgtgagaa ctcatcagag gctccttgcc agcagctgtt gcctcaaggg
      301 atctctgagc agattggagg cctacgagag ctcagcggcc tcaagaataa cctagacctc
      361 cagcaataca gctttattaa ccagctctgc tatgagactg ccctccactg gtacgccaag
      421 tacttcccct acctggtggt cattcatact cttatcttta tggtctgtac cagcttttgg
      481 ttcaaattcc ctggtaccag ctccaaaata gaacacttca tctctatcct gggcaagtgt
      541 ttcgactctc cgtggaccac tcgggccttg tctgaggtgt ctggagaaaa tcacaagggc
      601 ccagctgctg gacgggccac ggtgaccaca gtaaccacgg tggggacagg gacagggaag
      661 gtgggcgagg gtgagaagga gaaagtgctg atagagcctg agaaggtggt gactgaaccc
      721 ccagctgtaa ccctgctgga taagaaggag ggcgaacaag ctaaggccct atttgagaag
      781 gtcaaaaagt tccgagtgca cgtggaagag ggtgacatcc tctactccat gtacatccgg
      841 cagacggtgc tcaaagtgtg taagtttttt gccatcctgg tttacaacct ggtctatgtg
      901 gagaagatca gctttctggt ggcctgtagg gtggagacat cagagatcac aggctacgcc
      961 agtttctgct gcaaccacac caaggcccac ctcttttcta agctggcctt ctgctacatc
     1021 tccttcgtgt gtgtctatgg catcacctgc ctgtacacgc tctactggct cttccacagg
     1081 ccccttaaag agtactcctt ccgttctgtg cgggaggaga cagggatgaa tgatatcccg
     1141 gatgttagga atgacttcgc cttcatgctc cacctcattg accagtatga ctccctctat
     1201 tctaaacggt ttgcagtctt cctctctgaa gtcagtgaaa gccgccttaa gcagctcaac
     1261 ctcaaccacg agtggacacc tgataagctg aggcagaagc tgcagcgtaa cgcacgcggt
     1321 cggctggagc tgagcctctg tatgctccct gggcttccgg acactgtctt tgagctcagt
     1381 gaggtagagg cgctcagact ggaggccatc tgcgacatct cctttccacc agggctatcc
     1441 cagctcgtga accttcagga gctcagcctg cttcactcac cagccaggct gcccttctct
     1501 tcacagatct tcctgcgaga ccggctgaaa gtgatctgtg tcaagtttga ggagctccgc
     1561 gaggtacccc tctgggtgtt tgggctccga ggcttggaag aactacacct ggaaggactc
     1621 ttccccccag aaatggctcg gggtgccacc ctcgagagcc tccgggagct taaacagctc
     1681 aaggtactgt ctcttcgtag caatgcaggg aaggtgccag ccagcgtgac cgatgtagcc
     1741 gggcaccttc agcgactcag cctgcacaat gatggcgctc gtctgctcgc cttaaacagc
     1801 ctcaagaagc tggcggtgtt acgggagctg gagctggtgg cctgtgggct ggaacgtatc
     1861 ccccatgcaa tcttcagcct gggggctctg caggaactcg accttaagga caaccacctc
     1921 cggtctattg aggagatcct cagcttccag cactgtcgga agctggtcat tctcaggctg
     1981 tggcacaacc agatagccta tgtgcctgag catgtgagga agctccgaag cctggagcag
     2041 ctctacctca gtcacaacaa gcttgagact ctgcctgccc agcttggcca gtgctttggc
     2101 ctccgcctgc tggatgtgtc ccacaacggg cttcgttccc tgccacctga gttgggtctt
     2161 ctccagagtc tccagcatct agccctctcc tacaacgcac ttgagagcct gcctgatgaa
     2221 ctgttcttct gccataagct gcggacatta ctcttgggct acaaccacct gactcagttt
     2281 tcgccccacg tggctgccct tcaggccctc agccgtctgg agctcaaggg taacaggttg
     2341 gaggcactgc cagaagaact cggtgactgc aaggggctga aaaagtcagg gctcctggtg
     2401 gaggacaccc tttatgaggg gctgcctgca gaggttcggg agaaaatgga agaggaatga
     2461 agcctcttgg ggaacggggc aggatgacag agaggccttt atgtttctgc cccaacgttg
     2521 tattacaagg gagctgggtc cagcaggagt gggaggcagg ccagacatgt cggttttgtg
     2581 cagctcagac gagatcctgg gactggtttg tctgggggag aaacaggagg tggtggattt
     2641 ggggtgtaac gccttaggga aggattaact cagtcctagg ttcttcaacc aaaaccacac
     2701 ctgtgtcttt ggctggttgg ctcttcctct aaacattcca gcttagttag tttcctatac
     2761 aagagtgggg gacacatccc aatgggattt ccaacccttc cagcaacgat caacatatat
     2821 ttaaggagag gacacagggt ccacttccga agttctgtat cccaacttta atgccaagtc
     2881 cttatgttat aagctctttg tcacagggat gggtacttag aacaatggtt ctcacatgtg
     2941 gctatgtaat taaatcagca gaagggcccg gaagaaaata aaacctttac acgattc
//
