LOCUS       NM_001107204            6756 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus leucine rich repeat containing 8 VRAC subunit B
            (Lrrc8b), mRNA.
ACCESSION   NM_001107204 XM_001064039 XM_006250557 XM_223154
VERSION     NM_001107204.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Lutter,D., Ullrich,F., Lueck,J.C., Kempa,S. and Jentsch,T.J.
  TITLE     Selective transport of neurotransmitters and modulators by distinct
            volume-regulated LRRC8 anion channels
  JOURNAL   J Cell Sci 130 (6), 1122-1133 (2017)
   PUBMED   28193731
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   Voss,F.K., Ullrich,F., Munch,J., Lazarow,K., Lutter,D., Mah,N.,
            Andrade-Navarro,M.A., von Kries,J.P., Stauber,T. and Jentsch,T.J.
  TITLE     Identification of LRRC8 heteromers as an essential component of the
            volume-regulated anion channel VRAC
  JOURNAL   Science 344 (6184), 634-638 (2014)
   PUBMED   24790029
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000014.1.
            
            On or before Oct 3, 2022 this sequence version replaced
            XM_006250557.3, NM_001107204.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643284.16869.1, FQ217980.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760434 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-101               JAXUCZ010000014.1  4720976-4721076     c
            102-154             JAXUCZ010000014.1  4677630-4677682     c
            155-253             JAXUCZ010000014.1  4669058-4669156     c
            254-2415            JAXUCZ010000014.1  4663749-4665910     c
            2416-6756           JAXUCZ010000014.1  4655273-4659613     c
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14p22"
     gene            1..6756
                     /gene="Lrrc8b"
                     /gene_synonym="RGD1563429"
                     /note="leucine rich repeat containing 8 VRAC subunit B"
                     /db_xref="GeneID:305135"
                     /db_xref="RGD:1563429"
     exon            1..101
                     /gene="Lrrc8b"
                     /gene_synonym="RGD1563429"
                     /inference="alignment:Splign:2.1.0"
     exon            102..154
                     /gene="Lrrc8b"
                     /gene_synonym="RGD1563429"
                     /inference="alignment:Splign:2.1.0"
     exon            155..253
                     /gene="Lrrc8b"
                     /gene_synonym="RGD1563429"
                     /inference="alignment:Splign:2.1.0"
     exon            254..2415
                     /gene="Lrrc8b"
                     /gene_synonym="RGD1563429"
                     /inference="alignment:Splign:2.1.0"
     CDS             277..2688
                     /gene="Lrrc8b"
                     /gene_synonym="RGD1563429"
                     /note="leucine-rich repeat-containing protein 8B; leucine
                     rich repeat containing 8 family, member B"
                     /codon_start=1
                     /product="volume-regulated anion channel subunit LRRC8B"
                     /protein_id="NP_001100674.1"
                     /db_xref="GeneID:305135"
                     /db_xref="RGD:1563429"
                     /translation="MITLTELKCLADAQSSYHILKPWWDVFWYYLTLIMLLVAVLAGA
                     LQLTQSRVLCCLPCKVEFDNHCAVPWDLLKASENTSSNSGLPLPLPLRIQNDLHRQQY
                     SYIDAVCYEKQLHWFAKFFPYLVLLHTLIFAACSNFWLHYPSTSSRLEHFVSILHKCF
                     DSPWTTRALSETVAEQSVRPLKLSKSKTLLSTSGGSADIDASKQSLPYPQPGLESPGI
                     ESPTSSVLDKKEGEQAKAIFEKVKRFRLHVEQRDIIYRVYLKQIIVKVILFVLIITYV
                     PYFLSYITLEIDCSIDVQAFTGYKRYQCVYSLAEIFKVLASFYVILVMLYGLTSSYSL
                     WWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIFLSEVS
                     ENKLKQINLNNEWTVEKLKSKLVKNSQDKVELHLFMLNGLPDNVFELTDMEVLSLELI
                     PEVKLPAAASQLVNLRELRVYHSSLVVDHPALAFLEENLKILRLKFTEMGKIPRWVFH
                     LKNLKELYLSGCVLPEQLSSMQPEGFQDLKNLRTLYLKSSLSRIPQVVTDLLPSLQKL
                     SLDNEGSKLVVLNNLKKMVNLKSLELLSCDLERIPHSIFSLNNLHELDLKENNLKTVE
                     EIISFQHLPSLSCLKLWHNNIAYIPAQIGALSNLEQLYLGHNNIESLPLQLFLCTKLH
                     YLDLSYNHLTFIPEEIQYLTNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGRNSLTDL
                     SPLVGELSNLTHLELIGNYLETLPVELEGCQSLKRSCLIVEDSLLNSLPLPVTERLQT
                     CLDKC"
     exon            2416..6756
                     /gene="Lrrc8b"
                     /gene_synonym="RGD1563429"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcttcccgct gagccgcagc cgcctgcgga gtgcgggcgc tggccgctct gctcaccccg
       61 gcaggccggg tagcgcgggc cctgggggct ccagcgcaaa ggcattccgc agatcaaaac
      121 gaatcgcagt ttccggttga ccagaattca taggaatgaa gacaaagtca gagatggtgt
      181 gtttgagaaa cttcaaaagg tgtagacctc ctgactgaag cataattgat gtgactcatt
      241 tcttttcata atatttctcc tctctccacg ggcgtcatga ttacactaac agagttgaaa
      301 tgcttagcgg atgcccagtc atcctaccac atactgaagc cgtggtggga cgtcttctgg
      361 tattacctca ccctcatcat gctgttggtg gctgtgctgg ccggagccct gcagcttacg
      421 cagagcaggg ttctgtgctg tcttccctgc aaggtggaat ttgacaatca ctgcgctgtg
      481 ccttgggacc tcctgaaagc cagtgagaac acgtcttcta actcaggcct gccactcccg
      541 ctgccgctcc ggatccagaa cgacctccac cgacagcagt actcctacat cgacgccgtc
      601 tgctacgaga aacagctcca ttggttcgct aagtttttcc cctatctggt gcttctgcac
      661 actctaatct ttgcagcctg cagcaacttc tggcttcact accccagcac cagctccagg
      721 ctggagcact ttgtgtccat cctccacaag tgcttcgatt ctccatggac cactcgcgcc
      781 ctgtcagaga cagtggccga acagtcagtg aggcccctga aactctccaa gtccaaaacc
      841 ttgctctcaa cctcaggggg ctctgccgac atcgacgcca gcaagcagtc attgccctac
      901 ccacagccag gcttggagtc acctggcata gaaagcccaa cttctagcgt cctggacaag
      961 aaggagggtg aacaagccaa agccatcttc gaaaaagtga agaggtttcg cctgcatgtg
     1021 gagcagaggg acatcatcta ccgggtgtac ctgaagcaga taatcgtcaa agtcattctg
     1081 tttgttctca ttataactta cgtcccgtat tttctcagct acataactct ggaaatcgac
     1141 tgctcgatcg acgtgcaggc attcacaggc tataagcgct accagtgcgt ctactccttg
     1201 gcagagatct ttaaggtgtt ggcttcattt tatgtcattt tggtgatgct ttatggtctc
     1261 acgtcgtcct atagcctgtg gtggatgttg aggagttcct tgaagcagta ctcctttgag
     1321 gctctcagag agaaaagcaa ctacagcgat atccctgatg tcaagaacga cttcgccttc
     1381 atcctccatc tggccgacca gtacgacccc ctgtactcca agcgcttctc catcttcctg
     1441 tcggaggtca gtgagaacaa actgaagcag atcaacctca acaacgagtg gaccgtggag
     1501 aaactgaaga gcaagcttgt gaaaaactcc caggacaagg tcgagctgca tctcttcatg
     1561 ctcaacggtc ttccagataa cgtcttcgag ttaacagaca tggaagtgct gagcctggag
     1621 ctcatccccg aggtcaagct gcccgccgcc gcctctcagc ttgtcaacct cagggagctc
     1681 cgtgtgtacc actcgtcctt ggtggtcgac catcccgccc tggcctttct tgaggagaac
     1741 ttaaaaatcc tccgcctgaa atttaccgag atgggaaaga tcccacgctg ggtgttccac
     1801 ctgaagaacc tcaaggaact gtacctgtca ggctgtgtcc tcccggagca gctgagctcc
     1861 atgcagccgg agggcttcca ggatctgaag aacctgagga ccctctactt gaagagcagc
     1921 ctgtcccgga tccctcaggt ggtgacagac ctgctgccct ccctgcagaa gctgtccctc
     1981 gataacgagg ggagcaaact ggtcgtgctg aacaacctga agaagatggt caatctgaaa
     2041 agcctggagc tcctcagctg cgacctggag cgcatccccc actccatttt cagcctgaac
     2101 aacctgcacg agttggacct gaaggaaaac aacctcaaga cggtggagga gatcatcagc
     2161 ttccagcatc tcccgagcct ctcctgcctg aagctctggc acaataacat cgcttacatc
     2221 cccgcacaga tcggagcgct gtccaacctg gagcaactct acctgggtca caacaacatc
     2281 gagagcctgc ccttgcagct ttttctatgc accaaactac actacttgga tctaagctat
     2341 aaccatctga ccttcattcc cgaggaaatc cagtacctga ccaacctgca gtacttcgcc
     2401 gtgaccaaca ataacatcga gatgctacca gacgggctgt tccagtgcaa gaaacttcag
     2461 tgtctactct tgggaagaaa cagcctaaca gatttgtccc ctctggttgg ggagctgtca
     2521 aacctcaccc acctggaact cattggcaac tacctagaga cactgcctgt ggagctggaa
     2581 gggtgtcagt ccctgaaacg gagctgtctg attgtggagg acagcttgct taattctctt
     2641 ccgcttcctg tgacggagcg tttgcagact tgcttagata aatgttaacc taaagatcag
     2701 actcaagggg aaaaaaaaaa aaaaaagcga ttctaaagca gggccccagg aaaagagaac
     2761 gaactttcca aagttataaa gatgagagac ggcctgcaat tatgacacac cataaccaaa
     2821 aaatcttacc aaatgatcca gggtctgaat caagtaataa aaagatatca acactggggt
     2881 tttgtgaaca accaatgttt aaattatttg tgagatgtaa aaagtatacg ttgagggtgg
     2941 attgagaggc gtcgtcgtgg aaaccttacc tttcatgttt tgtcttcagg actctgtttc
     3001 caccactccc cctgccccct caacccatta cacgtttgca tacatgtttt catgtgacct
     3061 tgtaacctaa tatttaactc catgaagact catcagtata aactccttga tgtgtgctca
     3121 ccactggctt agatttatat tggtcttttt ttgttgttgt ttttttaagc agcgtgtatc
     3181 acactgggca gaattccatc ttggctgact tctctgctcc tttctcattt cgttggcatc
     3241 ttcatctggg tttctctgat agtcctggga tagggcttac acttggcatt tgcctagaac
     3301 gagagccagg atttctagga cgtgtgatct gctgagcagc ctaaaaatca atgcattctc
     3361 gaaatgtctt cttgtgtcat caaggccttc ttttactatg ttaaggattt ttatatgaaa
     3421 tatagaatat tttctactgt atgatctcag atgctacatc tgcgagaata cttttttttt
     3481 tttttgtaag cagtgaacat gtgcatcgtt gtgataattt aatgattctt ggtttgggct
     3541 gattatgatt cccgtgtttg tatgcactct gctccaaaaa cttttgtact cttgcaacta
     3601 attatggcaa ccaatttgga tgacaaaagc cttgtgtttt gctccctaac atctttgagg
     3661 gtttcctcat gagaggacag gagggccgct gaatcaccta cctctccttg ctgctgtcca
     3721 agcatttggg tacaacccct cagttacttt aaaactgtgt ttgaggggag aaggcaagat
     3781 ctagattaga aaagtgactg gaaaatacgt tgtagaaatt tagtaagcat taagtaagat
     3841 gaaggggtcc ccccccccat ctgcatgatc ccaaaatact attgtgatgg aagctgtcta
     3901 ttcagaacct gagcccatta aactgacatc catctcatag gctgccttgt gacctgagat
     3961 acgtcatgct cctcttgaca gagagctttg tgtttggccc tttgtcatca tgcgtattgc
     4021 tgaataaaac ggactggctc gctcacactg gggaaataga cattgtagtg tgtttttctc
     4081 tcaaaggtct aggcattatg aactcacctc tttttgggaa tagctgtcaa attatccatt
     4141 aactgcatct acaaaaaaac tggaaactac tatttacgag gctcctaaac acagagctgt
     4201 ggcatgtcat tttctcctgc ctgttctaga aagatgaaaa tgtctctcct cttaatatat
     4261 gcctgacatt tatagaagag aattacatgg aggaagcctg agtagagtta gttacaaaga
     4321 ctataccaag gccctaagct gcctaacaca ctatggatgg caagggatag actaaagaga
     4381 cgtcttagca aagcttgtct ttgttagaat gtccaagttc tcagggaccc aggcataact
     4441 ggctctcact ggggcctcat gagcaggccc tggcttagcc aggcttctct tattcttact
     4501 gttttaataa tgtgagttga tggttgacat tttccagtaa aatcatcatg tggaccaatt
     4561 tcaaaggtga cgaattgtct gaagttttga acataaatgt gagcgagagg tttcaatact
     4621 atcatccatg cctcatatga aataaaaaaa aaaaaaatcc accctaacca gttcagactt
     4681 tctaagcctc ccctgataaa ggagaaattc cctgtgcctt gttttccagt acatgcatca
     4741 ttggctaact ggtctacctc ctcaaagcag tttcctggaa ggatcaatca tgaagtgtga
     4801 accgctttca acacacaagg ctgtcgttac tggtggaaac catattgctc ttgatacaac
     4861 attttaaagt gtcttttgta tttaacactg atgaatcatt gctgaggtag cttttaagtc
     4921 taacactaag acaacgaaca acacaccacc tgacagacga atctgtgtaa acactggctt
     4981 tgctaacata actgtcgata gtcacctaac tgaagaaaat gaaacctttc atcatgagat
     5041 taacgtctca tgataatata ttttccaaaa actaagtgta aaggatcaaa aacaaaattt
     5101 tggcctatac taaaataata tgggttcatt tgcatacaca aatacagaga catgcacatg
     5161 cctcattttt gcaaacttca gttttcaata tattttttcc caaaaattgc aagaggttat
     5221 tataggttag cctgaggaga acaacttttt taattcatta cttttaatca cgaagaattc
     5281 aatttccata aaatcaactt ttagtttaag aaggtatcca gtgttcccac aaaggtggag
     5341 ttttcccctc atctgcatca gggtggattt ctgtagcata ttgatactga atttccattt
     5401 taaccttctg tatagtacca tatttctcta ttactaataa gcaggttgaa tcatggtatg
     5461 tgaaattgct ggactcagtg ataaaggcaa cctctaattt gtgtgctaca gtgtgctaaa
     5521 gtggtttgtg cacataccgt tattctttga tcatgaaatg gttctgtcca tgtttcataa
     5581 ttctgatatc atgttgaatt aaaacagcac atattattga tgggttcatt agtcaacgct
     5641 tactctgtgg acttgatact atttcctttc attttcagca aacccatcca tcttcgtagt
     5701 cacatagcgc ccgcccatct gcgcactgtg ttcttgccct cacccacctc actgaatctg
     5761 atggacactg tacagtagct gtgtgtccgt gctgtagtgt tggcagtaat acctgagctt
     5821 ggtcatccct gtttataaga atccagcttc tggtctctgt tctcattaac tgcctattgc
     5881 attagaatat gaaaaataaa ggagactaga gagagagaaa ggaagactta ttttgcatcc
     5941 tgaaggaaaa tatatatata tttagacagt gtaaacaagc aactgctgca gaacgtcctg
     6001 tgtgtaatag tgtctcgtgt gtccccatgt tctgtgctaa atcaggggag gctgcagaac
     6061 ggttcccttc ctgtctcttg tccatggctt ctacttccag tgacaaacgt ctgctatgta
     6121 ccttttgcaa tggactttat aaactgcatt cccaaacctt ttttttttaa atgagcaatt
     6181 tcatcaacac tgaagtatta acaaattaca tgagtcagag tactgagctg agatggcttc
     6241 tagttgagct taatttcata tttaagtttt gaatttatcg caaaatacat cctagctgac
     6301 aagaacaatt acgaatgcct tctgcatgtt cgttatttct aacagacatg gtgcaatttt
     6361 ttttaaatac aaaagtatat agggttggaa tggatgcctc tgcactcata tctgttgtgt
     6421 gtcagtcttt atattaacgc tgtacggtaa gacttaaaat tctaagtttc attctaagta
     6481 aaggatgggc caaccttgag tggagatact ttgctttgtt agctcccggt ggaggctggc
     6541 atttgatctt ttatctttta ccataaactt ggaaatgtgt atctggaatg aattactaga
     6601 aaatgcacaa ttatcttcaa tgtttttggc ccttggattg attcaaaccc ctacgttcga
     6661 tagatttggg taaattttgc tgtttttaaa gtatcttttg tgtaaagaga ttacattttt
     6721 ttataataaa tggcaactat gacaaatcgc tagaaa
//
