LOCUS       NM_001024782            3328 bp    mRNA    linear   ROD 08-DEC-2024
DEFINITION  Rattus norvegicus leucine rich repeat containing 8 VRAC subunit A
            (Lrrc8a), mRNA.
ACCESSION   NM_001024782 XM_231120
VERSION     NM_001024782.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Li,X.Y., Lv,X.F., Huang,C.C., Sun,L., Ma,M.M., Liu,C. and Guan,Y.Y.
  TITLE     LRRC8A is essential for volume-regulated anion channel in smooth
            muscle cells contributing to cerebrovascular remodeling during
            hypertension
  JOURNAL   Cell Prolif 54 (12), e13146 (2021)
   PUBMED   34725866
  REMARK    GeneRIF: LRRC8A is essential for volume-regulated anion channel in
            smooth muscle cells contributing to cerebrovascular remodeling
            during hypertension.
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Zhang,H., Liu,Y., Li,H., Li,J., Luo,Y. and Yan,X.
  TITLE     Novel insights into the role of LRRC8A in ameliorating alveolar
            fluid clearance in LPS induced acute lung injury
  JOURNAL   Eur J Pharmacol 861, 172613 (2019)
   PUBMED   31421089
  REMARK    GeneRIF: LRRC8A plays an essential role in the progression of AFC
            in LPS-induced acute lung injury
REFERENCE   3  (bases 1 to 3328)
  AUTHORS   Deneka,D., Sawicka,M., Lam,A.K.M., Paulino,C. and Dutzler,R.
  TITLE     Structure of a volume-regulated anion channel of the LRRC8 family
  JOURNAL   Nature 558 (7709), 254-259 (2018)
   PUBMED   29769723
REFERENCE   4  (bases 1 to 3328)
  AUTHORS   Schober,A.L., Wilson,C.S. and Mongin,A.A.
  TITLE     Molecular composition and heterogeneity of the LRRC8-containing
            swelling-activated osmolyte channels in primary rat astrocytes
  JOURNAL   J Physiol 595 (22), 6939-6951 (2017)
   PUBMED   28833202
REFERENCE   5  (bases 1 to 3328)
  AUTHORS   Syeda,R., Qiu,Z., Dubin,A.E., Murthy,S.E., Florendo,M.N.,
            Mason,D.E., Mathur,J., Cahalan,S.M., Peters,E.C., Montal,M. and
            Patapoutian,A.
  TITLE     LRRC8 Proteins Form Volume-Regulated Anion Channels that Sense
            Ionic Strength
  JOURNAL   Cell 164 (3), 499-511 (2016)
   PUBMED   26824658
REFERENCE   6  (bases 1 to 3328)
  AUTHORS   Voss,F.K., Ullrich,F., Munch,J., Lazarow,K., Lutter,D., Mah,N.,
            Andrade-Navarro,M.A., von Kries,J.P., Stauber,T. and Jentsch,T.J.
  TITLE     Identification of LRRC8 heteromers as an essential component of the
            volume-regulated anion channel VRAC
  JOURNAL   Science 344 (6184), 634-638 (2014)
   PUBMED   24790029
REFERENCE   7  (bases 1 to 3328)
  AUTHORS   Qiu,Z., Dubin,A.E., Mathur,J., Tu,B., Reddy,K., Miraglia,L.J.,
            Reinhardt,J., Orth,A.P. and Patapoutian,A.
  TITLE     SWELL1, a plasma membrane protein, is an essential component of
            volume-regulated anion channel
  JOURNAL   Cell 157 (2), 447-458 (2014)
   PUBMED   24725410
REFERENCE   8  (bases 1 to 3328)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   9  (bases 1 to 3328)
  AUTHORS   Sawada,A., Takihara,Y., Kim,J.Y., Matsuda-Hashii,Y., Tokimasa,S.,
            Fujisaki,H., Kubota,K., Endo,H., Onodera,T., Ohta,H., Ozono,K. and
            Hara,J.
  TITLE     A congenital mutation of the novel gene LRRC8 causes
            agammaglobulinemia in humans
  JOURNAL   J Clin Invest 112 (11), 1707-1713 (2003)
   PUBMED   14660746
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC097371.1.
            
            On Jun 10, 2005 this sequence version replaced XM_231120.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC097371.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3p12"
     gene            1..3328
                     /gene="Lrrc8a"
                     /gene_synonym="Lrrc8"
                     /note="leucine rich repeat containing 8 VRAC subunit A"
                     /db_xref="GeneID:311846"
                     /db_xref="RGD:1311690"
     exon            1..46
                     /gene="Lrrc8a"
                     /gene_synonym="Lrrc8"
                     /inference="alignment:Splign:2.1.0"
     exon            47..155
                     /gene="Lrrc8a"
                     /gene_synonym="Lrrc8"
                     /inference="alignment:Splign:2.1.0"
     exon            156..2320
                     /gene="Lrrc8a"
                     /gene_synonym="Lrrc8"
                     /inference="alignment:Splign:2.1.0"
     CDS             164..2596
                     /gene="Lrrc8a"
                     /gene_synonym="Lrrc8"
                     /note="leucine-rich repeat-containing protein 8A;
                     leucine-rich repeat-containing 8; leucine rich repeat
                     containing 8A; leucine rich repeat containing 8 family,
                     member A; swelling protein 1 SWELL1"
                     /codon_start=1
                     /product="volume-regulated anion channel subunit LRRC8A"
                     /protein_id="NP_001019953.1"
                     /db_xref="GeneID:311846"
                     /db_xref="RGD:1311690"
                     /translation="MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGT
                     LQVTQDKMICLPCKWVTKDSCNDSFRGWGASSPEPTYPNSTVLPTPDTGPTGIKYDLD
                     RHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSIL
                     LKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVPMLQRTKSR
                     IEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFF
                     LIICYTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGL
                     ICMYTLWWMLRRSLKKYSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAV
                     FLSEVSENKLRQLNLNNEWTLDKLRQRLTKNAQDKLELHLFMLSGIPDTVFDLLELEV
                     LKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFTDIKEI
                     PLWIYSLKTLEELHLTGNLSAENNRYIVIDGLRELKRLKVLRLKSNLSKLPQVVTDVG
                     VHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDN
                     NLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLF
                     YCRKLRYLDLSHNNLTLLPADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGN
                     NVLQSLPSRVGELTNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFSTLPPEV
                     KERLWRADKEQA"
     exon            2321..3315
                     /gene="Lrrc8a"
                     /gene_synonym="Lrrc8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggagggccaa ggctgcctgc ccggcggctg cgagcggcga gcccagggca gcagcagcgt
       61 ccatggagca aaaggaatgt caggaccctg tgcatccaga cggggaccag cctcagcacc
      121 aacagccaac acaaagatag cagagccatc tcagggttaa accatgattc cggtgacaga
      181 gctccgctac ttcgcggaca cgcagccagc ataccggatc ctgaagccgt ggtgggatgt
      241 gttcactgac tacatctcca ttgtcatgct gatgattgct gtctttgggg ggacactgca
      301 agtcacccag gacaagatga tctgcctacc ttgtaagtgg gtcaccaaag actcctgcaa
      361 cgactccttc cggggctggg gggcctccag cccagagccc acttacccca actccacagt
      421 cctgcctact cctgacacgg gccccacagg tatcaagtat gacctagatc gccaccagta
      481 caactacgtg gatgccgtgt gttacgagaa ccgcctgcat tggtttgcca agtacttccc
      541 ctacctcgtg ctcctgcaca ccctcatctt cctggcctgc agcaacttct ggttcaagtt
      601 cccacgcacc agctcgaagc tggagcactt cgtgtctatc ctgctcaagt gctttgattc
      661 gccatggacc acacgggccc tgtcagagac agtagtggag gagagtgacc ccaagccagc
      721 cttcagcaag atgaatggct ccatggacaa gaagtcatcc acggtcagtg aggatgtgga
      781 ggccactgtg cccatgctgc agcggaccaa gtcacggatt gagcagggca tcgtggaccg
      841 ctccgagaca ggtgtgctgg acaagaagga aggggagcag gcgaaggcac tgtttgagaa
      901 ggtgaagaag ttccggaccc atgtggagga gggggacatc gtgtaccgcc tctacatgcg
      961 gcagaccatc atcaaggtga tcaagttctt cctcatcatc tgctacaccg tgtactatgt
     1021 gcacaacatc aagtttgacg tggactgcac cgtggacatc gagagcctga caggctaccg
     1081 cacctaccgc tgtgcccacc ctctggccac cctcttcaag atcctggcat ccttctacat
     1141 cagcctggtc atcttctacg gcctcatctg catgtacacc ctgtggtgga tgctccggcg
     1201 ctccctcaag aagtactcgt ttgagtcgat ccgagaggag agcagctaca gcgacatccc
     1261 agacgtcaag aatgacttcg ccttcatgtt gcacctcatt gaccagtatg accctctcta
     1321 ctcaaagcgc ttcgccgtct tcctgtctga ggtgagtgag aacaagctgc gtcagctgaa
     1381 cctcaacaac gagtggacgc tggacaagct gcgccagcgg ctcaccaaaa acgcccagga
     1441 caagctggag ctgcacctct tcatgctgag cggcatcccg gacaccgtgt ttgacctgct
     1501 ggagttagag gtcctgaagc tggaactgat ccccgacgtg accatcccgc ccagcatcgc
     1561 ccagctcaca ggcctcaagg agctctggct gtaccacacg gcagccaaga tcgaggctcc
     1621 cgccctggcc ttcctgcgag agaacctgcg ggcgctgcac atcaagttca ctgacatcaa
     1681 ggagatccca ctgtggatct acagcctgaa gacactggag gagctgcact tgacaggcaa
     1741 cctgagcgca gagaacaacc gctacatcgt cattgacgga ctgcgggagc tcaaacgcct
     1801 taaggtgctg cggctcaaaa gcaacctgag caagctgccg caggtggtca cggacgtggg
     1861 cgtgcacctg cagaagctgt ccatcaacaa tgagggcacc aagctcatcg tcctcaacag
     1921 cctcaagaag atggttaacc tgacagagct ggagctgatc cgctgcgacc tggagcgtat
     1981 ccctcactcc atctttagcc tccacaacct gcaggagatc gacctgaagg acaacaacct
     2041 caagaccatc gaggagatca tcagcttcca gcatctgcac cgcctcacct gccttaagct
     2101 gtggtacaac cacatcgcct acatccccat ccagatcggc aacctcacca accttgagcg
     2161 cctctacctg aaccgcaaca aaatcgagaa aattcccacc cagctcttct actgccgcaa
     2221 gctgcgctac ctggacctca gccacaacaa cctgaccttg ctccccgctg acattggcct
     2281 cttgcagaac ctccagaacc tggccgtcac agccaatagg atcgaggcgc tccccccgga
     2341 gctcttccag tgtcggaagc tgcgggctct acacctgggc aacaatgttc tgcagtcact
     2401 gccctcgaga gtgggtgagc tgaccaacct gacgcagatt gagcttcggg gcaaccggct
     2461 ggagtgcctg cccgtggagc tgggcgagtg tcccctgctc aagcgcagcg gcctggtggt
     2521 agaggaggac ttgttcagca cactgccgcc tgaggtgaag gaacggctct ggagagctga
     2581 caaggagcag gcctgagcac agcctggagg agcagcacag agccgcagca caccctcagg
     2641 ccagcgggca gcccagctct ccccagactg ctggacagcc agccgggccc agccaaacag
     2701 aggctgggcc agagcagaaa acggggactg aggggctggc cccttttctc ctgctgggac
     2761 atgtacaccc agggcaggca cttgctggag accagcatct cagaatgcag tgtttggaca
     2821 atcagggtct tcacgctgga gccctctctg cctcagtgct gggctgagcc agcccccaag
     2881 caaggcccag gaacagtaac ctagatctgg gtatttattt ccacttccca ccttctcctt
     2941 ccagataact tacacattcc caagagggtc gggctctgtg ggcagtgttt agcaccctgg
     3001 cagtcacctt tttctttttc gggtgatact gacagaatca aatcaagtgc ccatccgtcc
     3061 agacttgggc agggttgggt agggtgagcc agccgcagga cggttgccgt attggcactg
     3121 ggctaggctc tggcagcaaa gcgtgcggga gaccaggcct ccaggttgaa gggtcaggcc
     3181 tgggcttgcc ccatcagttt ttgaagcaat tttagatttt tttttctttt ttttaagcaa
     3241 gggttttttg ggggggaggg agttggttgt ttttttaagc tttgaaatga tgggtgaggt
     3301 atttaaaaaa aaaaaaaaaa aaaaaaaa
//
