LOCUS       NM_024135               3455 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus LIM domain kinase 2 (Limk2), mRNA.
ACCESSION   NM_024135
VERSION     NM_024135.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Park,J., Son,H., Chai,J.S., Kim,S.W., Paick,J.S. and Cho,M.C.
  TITLE     Chronic administration of LIMK2 inhibitors alleviates cavernosal
            veno-occlusive dysfunction through suppression of cavernosal
            fibrosis in a rat model of erectile dysfunction after cavernosal
            nerve injury
  JOURNAL   PLoS One 14 (3), e0213586 (2019)
   PUBMED   30870492
  REMARK    GeneRIF: chronic inhibition of LIMK2 can improve cavernosal
            veno-occlusive dysfunction and ED by alleviating cavernosal
            fibrosis via normalizing the LIMK2/Cofilin pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Palmer,K., Fairfield,H., Borgeia,S., Curtain,M., Hassan,M.G.,
            Dionne,L., Yong Karst,S., Coombs,H., Bronson,R.T., Reinholdt,L.G.,
            Bergstrom,D.E., Donahue,L.R., Cox,T.C. and Murray,S.A.
  TITLE     Discovery and characterization of spontaneous mouse models of
            craniofacial dysmorphology
  JOURNAL   Dev Biol 415 (2), 216-227 (2016)
   PUBMED   26234751
REFERENCE   3  (bases 1 to 3455)
  AUTHORS   Ko,A.R., Hyun,H.W., Min,S.J., Kim,J.E. and Kang,T.C.
  TITLE     Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal
            death independent of NOS activity
  JOURNAL   Mol Brain 8, 58 (2015)
   PUBMED   26438559
  REMARK    GeneRIF: ET-1 may be one of the upstream effectors for programmed
            neuronal necrosis through abnormal LIMK2 over-expression by ROCK1.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3455)
  AUTHORS   Kim,J.E., Ryu,H.J., Kim,M.J. and Kang,T.C.
  TITLE     LIM kinase-2 induces programmed necrotic neuronal death via
            dysfunction of DRP1-mediated mitochondrial fission
  JOURNAL   Cell Death Differ 21 (7), 1036-1049 (2014)
   PUBMED   24561342
  REMARK    GeneRIF: Inhibition of LIMK2 expression and rescue of DRP1 function
            attenuates programmed necrotic neuronal death induced by status
            epilepticus.
REFERENCE   5  (bases 1 to 3455)
  AUTHORS   Li,Y., Hu,F., Chen,H.J., Du,Y.J., Xie,Z.Y., Zhang,Y., Wang,J. and
            Wang,Y.
  TITLE     LIMK-dependent actin polymerization in primary sensory neurons
            promotes the development of inflammatory heat hyperalgesia in rats
  JOURNAL   Sci Signal 7 (331), ra61 (2014)
   PUBMED   24962708
  REMARK    GeneRIF: Data indicate that LIM motif-containing protein kinases
            LIMK1 and LIMK2-dependent acin rearrangement is involved in the
            development of complete Freund's adjuvant (CFA)-induced
            inflammatory pain.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3455)
  AUTHORS   Sumi,T., Matsumoto,K., Takai,Y. and Nakamura,T.
  TITLE     Cofilin phosphorylation and actin cytoskeletal dynamics regulated
            by rho- and Cdc42-activated LIM-kinase 2
  JOURNAL   J Cell Biol 147 (7), 1519-1532 (1999)
   PUBMED   10613909
REFERENCE   7  (bases 1 to 3455)
  AUTHORS   Rosok,O., Pedeutour,F., Ree,A.H. and Aasheim,H.C.
  TITLE     Identification and characterization of TESK2, a novel member of the
            LIMK/TESK family of protein kinases, predominantly expressed in
            testis
  JOURNAL   Genomics 61 (1), 44-54 (1999)
   PUBMED   10512679
REFERENCE   8  (bases 1 to 3455)
  AUTHORS   Mori,T., Okano,I., Mizuno,K., Tohyama,M. and Wanaka,A.
  TITLE     Comparison of tissue distribution of two novel serine/threonine
            kinase genes containing the LIM motif (LIMK-1 and LIMK-2) in the
            developing rat
  JOURNAL   Brain Res Mol Brain Res 45 (2), 247-254 (1997)
   PUBMED   9149099
REFERENCE   9  (bases 1 to 3455)
  AUTHORS   Smolich,B., Vo,M., Buckley,S., Plowman,G. and Papkoff,J.
  TITLE     Cloning and biochemical characterization of LIMK-2, a protein
            kinase containing two LIM domains
  JOURNAL   J Biochem 121 (2), 382-388 (1997)
   PUBMED   9089416
REFERENCE   10 (bases 1 to 3455)
  AUTHORS   Nunoue,K., Ohashi,K., Okano,I. and Mizuno,K.
  TITLE     LIMK-1 and LIMK-2, two members of a LIM motif-containing protein
            kinase family
  JOURNAL   Oncogene 11 (4), 701-710 (1995)
   PUBMED   7651734
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from D31874.1.
            
            [WARNING] On Mar 18, 2024 this sequence was replaced by
            NM_024135.3.
            
            On Nov 29, 2004 this sequence version replaced NM_024135.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D31874.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14q21"
     gene            1..3455
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /note="LIM domain kinase 2"
                     /db_xref="GeneID:29524"
                     /db_xref="RGD:62056"
     exon            1..78
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..1979
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /EC_number="2.7.11.1"
                     /note="LIM motif-containing protein kinase 2; LIMK-2"
                     /codon_start=1
                     /product="LIM domain kinase 2"
                     /protein_id="NP_077049.2"
                     /db_xref="GeneID:29524"
                     /db_xref="RGD:62056"
                     /translation="MAALAGEEAWRCRGCGNYVPLSQRLYRTANEAWHSSCFRCSECQ
                     ESLTNWYYEKDGKLYCHKDYWAKFGEFCHGCSLLMTGPAMVAGEFKYHPECFACMSCK
                     VIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQDQLPYSVTLISMPATTE
                     CRRGFSVSVESASSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVE
                     EVEDAINQTSQTLQLLIEHDPVPQRLDQLRLDTRLSPHMQSSGHTLMLSTLDAKENQE
                     GTLRRRSLRRSNSISKSPGPSSPKEPLLLSRDISRSESLRCSSSYSQQIFRPCDLIHG
                     EVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFI
                     GVLYKDKKLNLLTEYIEGGTLKDFLRNVDPFPWQQKVRFAKGIASGMAYLHSMCIIHR
                     DLNSHNCLIKLDKTVVVADFGLSRLIVEERKRPPVEKAATKKRTLRKSDRKKRYTVVG
                     NPYWMAPEMLNGKSYDETVDVFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEK
                     FVPTDCPPAFFPLAAICCKLEPESRPAFSKLEDSFEALSLFLGELAIPLPAELEELDH
                     TVSMEYGLTRDSPP"
     exon            79..178
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     exon            179..314
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     exon            315..424
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     exon            425..613
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     exon            614..719
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     exon            720..916
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1103
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1190
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1322
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1379
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1445
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1620
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1676
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1834
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     exon            1835..3455
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3455
                     /gene="Limk2"
                     /gene_synonym="Limk2b; Link2"
ORIGIN      
        1 cgggcgcgga cctgaggtgc tgctgccggg tggtccttcc tgtgttgtcc gcgccctgga
       61 ccatggcggc gctggcgggt gaagaggcct ggaggtgtcg gggctgtggc aactatgttc
      121 ccctgagcca gaggctgtac aggacagcca acgaggcctg gcatagctcc tgcttccggt
      181 gttccgagtg ccaggagtcc ctcaccaact ggtactatga gaaggacggg aagctctact
      241 gccacaagga ctactgggcc aagtttggag aattctgcca cgggtgctcc ctgctgatga
      301 cagggccagc catggtggct ggggagttta agtaccaccc agagtgcttt gcctgcatga
      361 gttgcaaggt gatcatcgag gatggggatg cgtacgccct ggtgcagcat gctacactct
      421 actgcgggaa gtgccacaat gaggtggtgc tggcacccat gtttgaaaga ctgtctacgg
      481 agtctgtcca ggaccagctg ccctactctg tcacgctcat ctccatgcct gccaccaccg
      541 agtgcagacg tggcttctct gtgtctgtgg aaagcgcctc ctctaactat gccaccaccg
      601 tgcaagtgaa agaggtcaac cggatgcaca tcagccccaa caaccgaaat gccatccacc
      661 ccggggaccg catcttggag atcaatggga cccctgtccg cactctccga gtagaagagg
      721 tggaggatgc gattaaccag acgagccaga cacttcaact gctgattgaa catgaccctg
      781 tcccccagcg cctggaccag ttacggctag acacccggct ttctccccac atgcagagta
      841 gtggacatac tctcatgctc agcaccttgg acgccaagga aaatcaggag ggaacactga
      901 ggagacgttc tctgaggcgc agtaacagca tctccaagtc tcctggcccc agctccccca
      961 aggagccgct gctcctgagc cgagacatca gtcgctcaga gtcccttcgc tgctccagca
     1021 gctactcaca gcagatcttc cggccctgtg acctgatcca cggggaggtc ctggggaagg
     1081 gcttcttcgg acaggccatc aaggtgactc acaaagccac aggcaaagtg atggtcatga
     1141 aggagttaat tcgctgtgat gaggaaaccc agaagacttt cctgactgag gtgaaagtga
     1201 tgcgaagcct ggaccaccct aacgtgctta agttcatcgg ggtcctgtac aaggacaaga
     1261 agctgaatct gctgacagag tacattgagg ggggcaccct gaaggacttt ctgcgcaatg
     1321 tggacccgtt tccctggcaa cagaaggtca ggtttgccaa aggcatcgct tccggaatgg
     1381 cttatttaca ctccatgtgc atcatccacc gagatctgaa ctcgcacaac tgtctcatca
     1441 aattggacaa gacggtggtg gtagcagact tcgggctgtc acgtctcata gtggaagaga
     1501 ggaaaaggcc tccagtagag aaggccgcca ccaagaaacg caccttacgc aagagtgatc
     1561 gcaagaagcg ctacactgtg gtgggaaacc cctactggat ggcccctgag atgctgaatg
     1621 gcaagagcta cgatgagact gtggatgtct tctcttttgg gattgttctc tgtgagatca
     1681 ttgggcaggt atatgctgat cccgactgcc ttccccgcac actggacttc ggcctcaacg
     1741 taaagctttt ctgggagaag tttgtcccaa cagactgtcc cccagccttc ttccccctgg
     1801 ctgctatctg ctgcaaactg gagcctgaga gcagaccggc attctcaaag ctggaggact
     1861 cattcgaggc gctctccctg ttcttgggag agttggccat cccactgcca gcagagctgg
     1921 aagagctgga ccacactgtg agcatggagt atggcctaac ccgggactcg ccaccctagc
     1981 cctggtccat ccccctgcag ggggacattc cacagccagc attgccccct ctgcgccctg
     2041 tcctcgctgt gagcgagcaa gcgggactgc ctaggcttcc tgtggattga cggcttgctt
     2101 agaagcagaa caagccatcc ctacctcccc aggaggcact tgggtgcagg gaagcaattc
     2161 tccacaggtt tgggcctagt ttctgtctgt aaatccaaca ctcacctgaa agctgtgaag
     2221 aggataaaag cctggattct agaccaagag tctgttactg gggaccgccc gggacctctg
     2281 gactgagatg ccgggagcct gtagctttac actaacctac acggcctggg cccgccgggc
     2341 cggatgagtg gactggcctg agggcctctg gttcagcaag gtgcaggact tgaagcgata
     2401 gtatgagttt cagaaacggg gccgtcaaaa cgtgtgaaat gacagagcgg tttctccttc
     2461 catcctagcc atgccctttg gagcaggttt tgaagagtct tagtagtggt aaaacctaca
     2521 ggatccaaaa aaaaaggact ggtttctgct cagcagccca gggaccacat cagtgtgtgg
     2581 ggaatttcca cttcagcacc ctgtctcaac aagcagtctc aaaaacacca aagaaggtgg
     2641 ggctggaaag aaagcggccc tgccgtctaa aagccccaca cccaggctgg tgggctcagt
     2701 cagaaactta aggtgttgcc taacaataga tgggttctgg atgccagtgt ggtttgcctc
     2761 agactttggg cctaagcagg gagaaggggg tcccattaat ttctttgcac ccttgtggca
     2821 gctactgcat accctctagg atgtctggtc caggcagcaa cttgggacag gcattggtgg
     2881 ctgctgagtc ccaaactgag atatgaagag agctccctga gctggacctt gtagagtcta
     2941 cctctgttgt gctggtccag cttacttcct gagacacact cgagggccgt gggcatggga
     3001 aactctaggg gcactttcca cccctggcta ccaggtgtcc agggactccg tagctcacca
     3061 aggttcgcca tctcctaagt gggagctcac atcatattta acacattggg aatgggtttg
     3121 gttataaata cgtgtgaggg tttgtgctgg tggggggatc cggggagagt ggctgctctt
     3181 attagctttt atctgtgggg tgatatgtga aatattttgt acatagacaa gatgaattat
     3241 gggatgggaa gtctttggcc actttacttg ctgtgtagac tgtacttgtg tgtggagttt
     3301 gcaaacgtgc cgcagaggga atcctgaact cccgacatca gtgtggttca gcgtcctgcg
     3361 gcgaattatg cacgcgctgt ccccagcgag tgtgggagtg gtgggcctgc actgggccat
     3421 gaggcagact aaataaatta agcagttaac agagc
//
