LOCUS       NM_001106095            3192 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus DNA ligase 4 (Lig4), mRNA.
ACCESSION   NM_001106095 XM_001067682 XM_225045
VERSION     NM_001106095.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Hong,Y., Sonneville,R., Wang,B., Scheidt,V., Meier,B., Woglar,A.,
            Demetriou,S., Labib,K., Jantsch,V. and Gartner,A.
  TITLE     LEM-3 is a midbody-tethered DNA nuclease that resolves chromatin
            bridges during late mitosis
  JOURNAL   Nat Commun 9 (1), 728 (2018)
   PUBMED   29463814
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Felgentreff,K., Baxi,S.N., Lee,Y.N., Dobbs,K., Henderson,L.A.,
            Csomos,K., Tsitsikov,E.N., Armanios,M., Walter,J.E. and
            Notarangelo,L.D.
  TITLE     Ligase-4 Deficiency Causes Distinctive Immune Abnormalities in
            Asymptomatic Individuals
  JOURNAL   J Clin Immunol 36 (4), 341-353 (2016)
   PUBMED   27063650
REFERENCE   3  (bases 1 to 3192)
  AUTHORS   Craxton,A., Somers,J., Munnur,D., Jukes-Jones,R., Cain,K. and
            Malewicz,M.
  TITLE     XLS (c9orf142) is a new component of mammalian DNA double-stranded
            break repair
  JOURNAL   Cell Death Differ 22 (6), 890-897 (2015)
   PUBMED   25941166
REFERENCE   4  (bases 1 to 3192)
  AUTHORS   Oh,S., Harvey,A., Zimbric,J., Wang,Y., Nguyen,T., Jackson,P.J. and
            Hendrickson,E.A.
  TITLE     DNA ligase III and DNA ligase IV carry out genetically distinct
            forms of end joining in human somatic cells
  JOURNAL   DNA Repair (Amst) 21, 97-110 (2014)
   PUBMED   24837021
REFERENCE   5  (bases 1 to 3192)
  AUTHORS   Kuhmann,C., Li,C., Kloor,M., Salou,M., Weigel,C., Schmidt,C.R.,
            Ng,L.W., Tsui,W.W., Leung,S.Y., Yuen,S.T., Becker,N.,
            Weichenhan,D., Plass,C., Schmezer,P., Chan,T.L. and Popanda,O.
  TITLE     Altered regulation of DNA ligase IV activity by aberrant promoter
            DNA methylation and gene amplification in colorectal cancer
  JOURNAL   Hum Mol Genet 23 (8), 2043-2054 (2014)
   PUBMED   24282031
REFERENCE   6  (bases 1 to 3192)
  AUTHORS   Frank,K.M., Sekiguchi,J.M., Seidl,K.J., Swat,W., Rathbun,G.A.,
            Cheng,H.L., Davidson,L., Kangaloo,L. and Alt,F.W.
  TITLE     Late embryonic lethality and impaired V(D)J recombination in mice
            lacking DNA ligase IV
  JOURNAL   Nature 396 (6707), 173-177 (1998)
   PUBMED   9823897
REFERENCE   7  (bases 1 to 3192)
  AUTHORS   Grawunder,U., Zimmer,D., Fugmann,S., Schwarz,K. and Lieber,M.R.
  TITLE     DNA ligase IV is essential for V(D)J recombination and DNA
            double-strand break repair in human precursor lymphocytes
  JOURNAL   Mol Cell 2 (4), 477-484 (1998)
   PUBMED   9809069
REFERENCE   8  (bases 1 to 3192)
  AUTHORS   Nagaki,S., Yamamoto,M., Yumoto,Y., Shirakawa,H., Yoshida,M. and
            Teraoka,H.
  TITLE     Non-histone chromosomal proteins HMG1 and 2 enhance ligation
            reaction of DNA double-strand breaks
  JOURNAL   Biochem Biophys Res Commun 246 (1), 137-141 (1998)
   PUBMED   9600082
REFERENCE   9  (bases 1 to 3192)
  AUTHORS   Grawunder,U., Wilm,M., Wu,X., Kulesza,P., Wilson,T.E., Mann,M. and
            Lieber,M.R.
  TITLE     Activity of DNA ligase IV stimulated by complex formation with
            XRCC4 protein in mammalian cells
  JOURNAL   Nature 388 (6641), 492-495 (1997)
   PUBMED   9242410
REFERENCE   10 (bases 1 to 3192)
  AUTHORS   Robins,P. and Lindahl,T.
  TITLE     DNA ligase IV from HeLa cell nuclei
  JOURNAL   J Biol Chem 271 (39), 24257-24261 (1996)
   PUBMED   8798671
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473970.2.
            
            [WARNING] On Oct 26, 2024 this sequence was replaced by
            NM_001106095.2.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_225045.4, XM_001067682.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: CB734948.1, CO395037.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMD00132261, SAMD00132267 [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16q12.5"
     gene            1..3192
                     /gene="Lig4"
                     /note="DNA ligase 4"
                     /db_xref="GeneID:290907"
                     /db_xref="RGD:1304639"
     exon            1..253
                     /gene="Lig4"
                     /inference="alignment:Splign:2.1.0"
     exon            254..3192
                     /gene="Lig4"
                     /inference="alignment:Splign:2.1.0"
     CDS             283..3018
                     /gene="Lig4"
                     /EC_number="6.5.1.1"
                     /note="DNA ligase (ATP) 4; ligase IV, DNA, ATP-dependent"
                     /codon_start=1
                     /product="DNA ligase 4"
                     /protein_id="NP_001099565.1"
                     /db_xref="GeneID:290907"
                     /db_xref="RGD:1304639"
                     /translation="MASSQASRTVAAHVPFADLCSTLERIQKGKDRAEKIRHFKEFLD
                     SWRKFHDALHKNKKDVPDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPRE
                     GKDAQKLLNYRTPSGARSDAGDFATIAYFVLKPRCLQKGSLTIQKVNELLDLVASNNS
                     GKRKDLVKKSLLQLITQCSALEQKWLIRMIIKDLKLGVSQHTILSIFHNDAVELHNVT
                     TDLEKVCRQLHDPSVGLSDISITLFSAFRPMLAAVADVERVEKDMKQQSFYIETKLDG
                     ERMQMHKDGALYRYFSRNGYNYTDQFGESPQEGSLTPFIHNAFKTDVQVCILDGEMMA
                     YNPTTQTFMQKGVKFDIKRMVEDSDLQTCYCVFDVLMVNNKKLGRETLRKRYDILSST
                     FTPIQGRIEIVQKTQAQTKQEVVDALNDAIDKREEGIMVKHPLSIYKPDKRGEGWLKI
                     KPEYVTGLMDELDLLIVGGYWGKGSRGGMMSHFLCAVAETPPPGDKPSVFHTLCRVGS
                     GYTMKELYDLGLKLAKYWKPFQRKSPPSCILCGTEKPEVYIEPQNSVIVQIKAAEIVP
                     SDMYKTGSTLRFPRIEKIRDDKEWHDCMTLDDLEQLRGKASGKLATKHLHIGDDDEPR
                     EKRRKPISKTKKTIGIIEHLKAPNLSNINKVSNVFEDVEFCVMSGLDGYPKPDLENKI
                     AEFGGYIVQNPGPDTYCVIAGSENIRVRNIISSDRHDVVKPEWLLECFKTKTCVPWQP
                     RFMIHMCPSTKQHFAREYDCYGDSYFVDADLDQLKEVFLRIKPSEQQTPEEMAPVIAD
                     LECRYSWDHSPLSMFRHYTVYLDLYAVINDLSSKIEATRLGVTALELRFRGAKVVSRL
                     SEGVSHVIIGEDQRRVSDFKTFRRTLKKKFKILQERWVIDSVDKCELQEENLYLL"
ORIGIN      
        1 taggcaagtc gtgtggcggg ctgtcaggtc ctagcgtcac caccccggag cttctcgtgg
       61 ggcgacgcct tccgcggtgc agccggctcg ctgcggactc gtttcaggtg gcagccaccg
      121 ggtgaggaaa tcttgttctg ataattagtt gtgtacattc tgaaagtgaa gcctccctct
      181 gccgcctagc tacctaggac ggccgcactc ctccagcttc cttcggctgg actgggattt
      241 acaggttgct tagttaaaac cggaggattt gttgtcgttt ctatggcttc ctcacaagcc
      301 tcacgaactg tcgcagctca tgtccccttt gcagacttgt gctccacctt agaacgaata
      361 cagaaaggta aagaccgtgc agaaaaaatc aggcacttca aggagtttct ggattcttgg
      421 agaaaatttc atgatgccct tcataagaac aagaaggatg ttccggactc tttttaccca
      481 gcaatgagac ttattctccc tcagctagag agagagagga tggcttacgg aatcaaagaa
      541 accatgctgg ctaagcttta catcgaattg ctcaatttac caagagaagg caaggatgcc
      601 cagaagctcc tgaattaccg aacccccagt ggagctcgct cagatgctgg ggactttgcc
      661 acgattgcat actttgtttt gaagccaagg tgcttacaga aaggaagctt aaccatacag
      721 aaggtgaatg aactcttaga cttagttgcc agcaataact ctggcaaaag aaaagatcta
      781 gtgaaaaaga gccttcttca gttaataacc cagtgttcag cactcgagca aaagtggctg
      841 attcgcatga ttattaagga cttaaagctt ggtgtcagtc agcacacgat attgtccatc
      901 ttccacaatg atgcagtcga gttgcacaat gtcaccacag atctggaaaa ggtctgtagg
      961 cagctacatg acccctccgt agggctcagc gacatctcca tcactttgtt ctctgccttt
     1021 aggccaatgc tagctgctgt agcagatgtg gagcgtgtgg agaaggacat gaagcagcag
     1081 agtttctaca tcgaaactaa gctcgatggc gagcgcatgc agatgcacaa ggatggcgcc
     1141 ctgtaccggt acttctccag aaatggttac aactacaccg atcagttcgg cgaatctcca
     1201 caggaaggct ctctcacccc gtttattcac aacgcattca agacagatgt gcaagtgtgc
     1261 atccttgatg gcgagatgat ggcctacaac ccaacgacac agactttcat gcagaagggg
     1321 gtcaagtttg atatcaaaag gatggtcgaa gattctgacc tacagacatg ctactgtgtt
     1381 ttcgatgtgc tgatggttaa taataagaag ctagggcgtg agactctcag gaagaggtat
     1441 gatatcctta gtagcacttt cacacccata caaggtcgaa tagagatagt gcagaaaact
     1501 caagctcaga caaagcagga agtagtggat gcattaaatg atgccataga caagagagaa
     1561 gaggggatca tggttaaaca ccctctgtcc atttacaagc cagacaaaag aggtgaaggg
     1621 tggctaaaga ttaaaccaga gtatgtcact ggattgatgg atgaattaga cctcttaatt
     1681 gttgggggct actggggtaa aggttctcga ggtggcatga tgtctcactt tctgtgtgca
     1741 gtggcagaga caccacctcc tggtgacaag ccatctgtgt tccacactct gtgccgtgtt
     1801 gggtcgggtt acacgatgaa agaactctat gacctgggct tgaaattggc aaaatactgg
     1861 aagcctttcc aaagaaaatc tccaccaagc tgtattttgt gtggcacaga gaagccagaa
     1921 gtgtacattg aaccccagaa ctctgtcatc gttcagatca aggcggcaga gatcgtcccc
     1981 agtgacatgt acaagactgg ctctaccttg cgcttcccac gcatcgagaa gatcagagat
     2041 gacaaagagt ggcatgactg tatgacgctg gatgacctgg agcagctgag ggggaaagca
     2101 tctgggaagc ttgccacaaa acaccttcac ataggtgacg atgacgaacc cagagaaaaa
     2161 aggcggaaac ccatctctaa gacgaagaaa accattggaa tcattgaaca cctaaaagcg
     2221 cctaaccttt ctaacataaa caaagtttct aatgtatttg aagatgttga gttttgtgtc
     2281 atgagtggat tagatggtta tccaaagcct gacctagaga acaaaattgc agaatttggt
     2341 ggttatatag tccagaatcc aggcccagat acatactgtg tgattgcagg ttctgagaac
     2401 attagggtca gaaacatcat ctcttcagat aggcatgatg ttgtcaagcc cgagtggctt
     2461 ttagagtgtt ttaaaacaaa aacatgtgtg ccatggcaac cccgctttat gattcacatg
     2521 tgcccatcaa caaagcagca ttttgcccgt gaatatgact gctatggtga tagctatttc
     2581 gttgatgcag atttggatca attgaaagaa gtgtttttaa gaattaaacc cagtgagcag
     2641 cagactcctg aagaaatggc ccctgtgatt gctgacctag aatgtcgtta ttcctgggat
     2701 cactctcctc tcagtatgtt tcgacactac accgtttatt tggacttgta tgctgttatt
     2761 aatgacttaa gttccaaaat cgaagccaca aggttaggtg ttacagcact tgagctgcgg
     2821 tttcgtggag caaaggtggt ttcccgctta tctgaagggg tgtctcacgt aatcatcggg
     2881 gaagatcaga gacgagtcag tgactttaaa acgttcagaa gaacgcttaa gaaaaagttt
     2941 aaaatcctgc aggaacgttg ggtgatcgat tcagtagaca agtgtgaact gcaggaagaa
     3001 aacctgtatt tgctttagag gaaaatgtta accgtgtcag acaacagcag gcagtgatga
     3061 tggaatagcc agtggccttc attttatctc ctaatacttc aaaaggatta gttaccagaa
     3121 ggcaatactt ttcaagtaga aaggaaactg aataggccaa agccaaaaaa acccttttcc
     3181 caacataact ta
//
