LOCUS       NM_001012037            1636 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus lipoic acid synthetase (Lias), mRNA; nuclear gene
            for mitochondrial product.
ACCESSION   NM_001012037 XM_223409
VERSION     NM_001012037.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Yi,X., Kim,K., Yuan,W., Xu,L., Kim,H.S., Homeister,J.W., Key,N.S.
            and Maeda,N.
  TITLE     Mice with heterozygous deficiency of lipoic acid synthase have an
            increased sensitivity to lipopolysaccharide-induced tissue injury
  JOURNAL   J Leukoc Biol 85 (1), 146-153 (2009)
   PUBMED   18845616
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Garcia-Garcia,M.J., Eggenschwiler,J.T., Caspary,T., Alcorn,H.L.,
            Wyler,M.R., Huangfu,D., Rakeman,A.S., Lee,J.D., Feinberg,E.H.,
            Timmer,J.R. and Anderson,K.V.
  TITLE     Analysis of mouse embryonic patterning and morphogenesis by forward
            genetics
  JOURNAL   Proc Natl Acad Sci U S A 102 (17), 5913-5919 (2005)
   PUBMED   15755804
REFERENCE   3  (bases 1 to 1636)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   4  (bases 1 to 1636)
  AUTHORS   Morikawa,T., Yasuno,R. and Wada,H.
  TITLE     Do mammalian cells synthesize lipoic acid? Identification of a
            mouse cDNA encoding a lipoic acid synthase located in mitochondria
  JOURNAL   FEBS Lett 498 (1), 16-21 (2001)
   PUBMED   11389890
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC083708.1.
            
            On Feb 11, 2005 this sequence version replaced XM_223409.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC083708.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14p11"
     gene            1..1636
                     /gene="Lias"
                     /note="lipoic acid synthetase"
                     /db_xref="GeneID:305348"
                     /db_xref="RGD:1307270"
     exon            1..108
                     /gene="Lias"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..1185
                     /gene="Lias"
                     /EC_number="2.8.1.8"
                     /note="LS; lip-syn; lipoate synthase; lipoic acid
                     synthase; lipoyl synthase, mitochondrial"
                     /codon_start=1
                     /product="lipoyl synthase, mitochondrial precursor"
                     /protein_id="NP_001012037.1"
                     /db_xref="GeneID:305348"
                     /db_xref="RGD:1307270"
                     /translation="MALRCWDAARSLGSRIFGRYACSVRALSSLPDEKKKFLHNGPDL
                     QDFVSGDLADKSTWDDYKGNLKRQKGERLRLPPWLKTKIPMGKNYNKLKNTLRNLNLH
                     TVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGCRFCSVKTARNPPPLDPSEPD
                     NTARAIAEWGLDYVVLTSVDRDDVVDGGAEHIAKTVSCLKERNPKILVECLTPDFRGD
                     LRAVEKVALSGLDVYAHNVETVPELQRKVRDPRANFDQSLRVLKHAKEVQPDVVSKTS
                     IMLGLGETDEQVYATMKALRAADVDCLTLGQYMQPTKRHLKVEEYVTPEKFKYWEEVG
                     NELGFHYTASGPLVRSSYKAGEFFLKNLVAKRKTKVSKV"
     exon            109..278
                     /gene="Lias"
                     /inference="alignment:Splign:2.1.0"
     exon            279..372
                     /gene="Lias"
                     /inference="alignment:Splign:2.1.0"
     exon            373..453
                     /gene="Lias"
                     /inference="alignment:Splign:2.1.0"
     exon            454..668
                     /gene="Lias"
                     /inference="alignment:Splign:2.1.0"
     exon            669..797
                     /gene="Lias"
                     /inference="alignment:Splign:2.1.0"
     exon            798..943
                     /gene="Lias"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1014
                     /gene="Lias"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1126
                     /gene="Lias"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1587
                     /gene="Lias"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccgtcctgtt ccgggggtgc tggttcctcg gtcccttgac cctcctaaga tctaaaagag
       61 gggatggctt tacgctgctg ggatgcagct cgcagcctgg ggtcccggat atttggaaga
      121 tatgcgtgct cggttagagc attaagttct ttgccagatg agaaaaagaa atttttgcat
      181 aatgggccag accttcaaga ttttgtatct ggtgatcttg cagacaagag tacctgggat
      241 gattataaag gaaacttaaa gcgccagaaa ggagaaaggt taagactacc tccgtggtta
      301 aagacaaaga tacccatggg taaaaattac aataagctga aaaatacgtt gcggaattta
      361 aatctccaca cagtatgtga ggaagcccga tgtccaaaca tcggagagtg ttggggaggt
      421 ggggaatatg ccaccgccac agccacgatc atgttgatgg gggacacatg cacaagaggt
      481 tgcagatttt gctctgttaa gacggcaaga aatccccctc cattggatcc cagtgagccc
      541 gacaatacgg ccagagcaat cgcagagtgg ggtctggact atgttgtgct gacgtctgtg
      601 gaccgagatg atgtggtcga tgggggagct gagcacattg ccaagacagt gtcatgcttg
      661 aaggaaagga atccaaaaat cctcgtggaa tgcctcaccc ccgacttccg aggagatctg
      721 agagctgtag aaaaggtggc gctgtctgga ctagatgtgt atgcacacaa tgtagagacg
      781 gttccagaat tacagaggaa agtccgtgat ccccgggcca attttgacca gtctcttcgt
      841 gtattgaaac atgccaagga ggtgcagcct gatgttgttt ccaaaacatc aataatgctg
      901 ggcctcggtg agacagacga gcaggtctat gccacgatga aagctctccg tgcagccgat
      961 gtggactgtt taactctcgg gcagtatatg cagccgacca agcgccacct taaggttgaa
     1021 gagtacgtta ctcctgagaa gttcaaatac tgggaagaag taggaaatga acttggattt
     1081 cactacactg caagtggccc tttggtgcga tcttcatata aagcaggtga atttttcctg
     1141 aaaaatctag tggctaaaag aaaaacaaaa gtttctaaag tttagccaag accttccaga
     1201 tcattggaat gttaagattt cacttccggt tggtgtgaga gcggtgccat cctgtcagga
     1261 ttcctgtgca tgcaaaccag cacctggtct gaaatgtctg atgtctggtc caggcataat
     1321 ggcatatacc tgtaagtcca cccaggccat cctgacttcc tgcggaagac ccgatctcaa
     1381 aaaaaggaaa agaaagagaa aggaagaaag aaaaccgtct gtaagctata cacacttagt
     1441 gatgataatt catttgactt cttaccaacc tttgtcctca tccttgtcag tgatgtgatc
     1501 atggaacatc tttgaatggt ttgctgtggc cattgtaatt taaaagtgta ttaacattta
     1561 gaagatctta taaatcacag taacccaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1621 aaaaaaaaaa aaaaaa
//
