LOCUS       NM_173328               4648 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus leucine-rich repeat-containing G protein-coupled
            receptor 4 (Lgr4), mRNA.
ACCESSION   NM_173328
VERSION     NM_173328.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Wang,M., Liu,J., Zhu,G. and Chen,X.
  TITLE     Low levels of cadmium exposure affect bone by inhibiting Lgr4
            expression in osteoblasts and osteoclasts
  JOURNAL   J Trace Elem Med Biol 73, 127025 (2022)
   PUBMED   35772369
  REMARK    GeneRIF: Low levels of cadmium exposure affect bone by inhibiting
            Lgr4 expression in osteoblasts and osteoclasts.
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Hsu,P.J., Wu,F.J., Kudo,M., Hsiao,C.L., Hsueh,A.J. and Luo,C.W.
  TITLE     A naturally occurring Lgr4 splice variant encodes a soluble
            antagonist useful for demonstrating the gonadal roles of Lgr4 in
            mammals
  JOURNAL   PLoS One 9 (9), e106804 (2014)
   PUBMED   25188337
  REMARK    GeneRIF: The endogenously expressed Lgr4 may act as an antagonist
            molecule that helps to fine-tune the R-spondin/norrin-mediated
            Lgr4-Wnt signaling during gonadal development.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4648)
  AUTHORS   Kinzel,B., Pikiolek,M., Orsini,V., Sprunger,J., Isken,A.,
            Zietzling,S., Desplanches,M., Dubost,V., Breustedt,D., Valdez,R.,
            Liu,D., Theil,D., Muller,M., Dietrich,B., Bouwmeester,T.,
            Ruffner,H. and Tchorz,J.S.
  TITLE     Functional roles of Lgr4 and Lgr5 in embryonic gut, kidney and skin
            development in mice
  JOURNAL   Dev Biol 390 (2), 181-190 (2014)
   PUBMED   24680895
REFERENCE   4  (bases 1 to 4648)
  AUTHORS   Wang,F., Zhang,X., Wang,J., Chen,M., Fan,N., Ma,Q., Liu,R.,
            Wang,R., Li,X., Liu,M. and Ning,G.
  TITLE     LGR4 acts as a link between the peripheral circadian clock and
            lipid metabolism in liver
  JOURNAL   J Mol Endocrinol 52 (2), 133-143 (2014)
   PUBMED   24353284
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4648)
  AUTHORS   Li,J.Y., Chai,B., Zhang,W., Fritze,D.M., Zhang,C. and
            Mulholland,M.W.
  TITLE     LGR4 and its ligands, R-spondin 1 and R-spondin 3, regulate food
            intake in the hypothalamus of male rats
  JOURNAL   Endocrinology 155 (2), 429-440 (2014)
   PUBMED   24280058
  REMARK    GeneRIF: These data indicate that Rspo1 and Rspo3 and their
            receptor LGR4 form novel circuits in the brain to regulate energy
            homeostasis.
REFERENCE   6  (bases 1 to 4648)
  AUTHORS   Carmon,K.S., Gong,X., Lin,Q., Thomas,A. and Liu,Q.
  TITLE     R-spondins function as ligands of the orphan receptors LGR4 and
            LGR5 to regulate Wnt/beta-catenin signaling
  JOURNAL   Proc Natl Acad Sci U S A 108 (28), 11452-11457 (2011)
   PUBMED   21693646
REFERENCE   7  (bases 1 to 4648)
  AUTHORS   Mohri,Y., Oyama,K., Akamatsu,A., Kato,S. and Nishimori,K.
  TITLE     Lgr4-deficient mice showed premature differentiation of ureteric
            bud with reduced expression of Wnt effector Lef1 and Gata3
  JOURNAL   Dev Dyn 240 (6), 1626-1634 (2011)
   PUBMED   21523854
REFERENCE   8  (bases 1 to 4648)
  AUTHORS   Luo,J., Zhou,W., Zhou,X., Li,D., Weng,J., Yi,Z., Cho,S.G., Li,C.,
            Yi,T., Wu,X., Li,X.Y., de Crombrugghe,B., Hook,M. and Liu,M.
  TITLE     Regulation of bone formation and remodeling by G-protein-coupled
            receptor 48
  JOURNAL   Development 136 (16), 2747-2756 (2009)
   PUBMED   19605502
REFERENCE   9  (bases 1 to 4648)
  AUTHORS   Mendive,F., Laurent,P., Van Schoore,G., Skarnes,W., Pochet,R. and
            Vassart,G.
  TITLE     Defective postnatal development of the male reproductive tract in
            LGR4 knockout mice
  JOURNAL   Dev Biol 290 (2), 421-434 (2006)
   PUBMED   16406039
REFERENCE   10 (bases 1 to 4648)
  AUTHORS   Hsu,S.Y., Liang,S.G. and Hsueh,A.J.
  TITLE     Characterization of two LGR genes homologous to gonadotropin and
            thyrotropin receptors with extracellular leucine-rich repeats and a
            G protein-coupled, seven-transmembrane region
  JOURNAL   Mol Endocrinol 12 (12), 1830-1845 (1998)
   PUBMED   9849958
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Dec 7, 2020 this sequence version replaced NM_173328.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF061443.1, SRR21887622.3266350.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-665               JAXUCZ010000003.1  116901968-116902632
            666-737             JAXUCZ010000003.1  116954456-116954527
            738-809             JAXUCZ010000003.1  116973158-116973229
            810-881             JAXUCZ010000003.1  116974449-116974520
            882-1097            JAXUCZ010000003.1  116986567-116986782
            1098-1169           JAXUCZ010000003.1  116987830-116987901
            1170-1238           JAXUCZ010000003.1  116989364-116989432
            1239-1310           JAXUCZ010000003.1  116990241-116990312
            1311-1382           JAXUCZ010000003.1  116990511-116990582
            1383-1451           JAXUCZ010000003.1  116990911-116990979
            1452-1523           JAXUCZ010000003.1  116992431-116992502
            1524-1589           JAXUCZ010000003.1  116993447-116993512
            1590-1661           JAXUCZ010000003.1  116994538-116994609
            1662-1733           JAXUCZ010000003.1  116996924-116996995
            1734-1859           JAXUCZ010000003.1  116997562-116997687
            1860-1975           JAXUCZ010000003.1  116998479-116998594
            1976-2059           JAXUCZ010000003.1  116998944-116999027
            2060-4648           JAXUCZ010000003.1  117001565-117004153
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q34"
     gene            1..4648
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /note="leucine-rich repeat-containing G protein-coupled
                     receptor 4"
                     /db_xref="GeneID:286994"
                     /db_xref="RGD:628615"
     exon            1..665
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     CDS             481..3336
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /note="G-protein coupled receptor 48"
                     /codon_start=1
                     /product="leucine-rich repeat-containing G-protein coupled
                     receptor 4 precursor"
                     /protein_id="NP_775450.2"
                     /db_xref="GeneID:286994"
                     /db_xref="RGD:628615"
                     /translation="MPGPLRLLCFFALGLLGSAGPSGAAPPLCAAPCSCDGDRRVDCS
                     GKGLTAVPEGLSAFTQALDISMNNITQLPEDAFKSFPFLEELQLAGNDLSFIHPKALS
                     GLKELKVLTLQNNQLRTVPSEAIHGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWL
                     DDNSLTEVPVRPLSNLPTLQALTLALNNISSIPDFAFTNLSSLVVLHLHNNKIKSLSQ
                     HCFDGLDNLETLDLNYNNLDEFPQAIKALPSLKELGFHSNSISVIPDGAFGGNPLLRT
                     IHLYDNPLSFVGNSAFHNLSDLHSLVIRGASLVQWFPNLTGTVHLESLTLTGTKISSI
                     PDDLCQNQKMLRTLDLSYNNIRDLPSFNGCRALEEISLQRNQISLIKENTFQGLTSLR
                     ILDLSRNLIREIHSGAFAKLGTITNLDVSFNELTSFPTEGLNGLNQLKLVGNFKLKDA
                     LAARDFANLRSLSVPYAYQCCAFWGCDSYANLNTEDNSPQEHSVTKEKGATDAANVTS
                     TAENEEHSQIIIHCTPSTGAFKPCEYLLGSWMIRLTVWFIFLVALLFNLLVILTVFAS
                     CSSLPASKLFIGLISVSNLLMGIYTGILTFLDAVSWGRFAEFGIWWETGSGCKVAGSL
                     AVFSSESAVFLLTLAAVERSVFAKDLMKHGKSSHLRQFQVAALLALLGAAVAGCFPLF
                     HGGQYSASPLCLPFPTGETPSLGFTVTLVLLNSLAFLLMAIIYTKLYCNLEKEDLSEN
                     SQSSVIKHVAWLIFTNCIFFCPVAFFSFAPLITAISISPEIMKSVTLIFFPLPACLNP
                     VLYVFFNPKFKEDWKLLKRRVTRKHGSVSVSISSQGGCGEQDFYYDCGMYSHLQGNLT
                     VCDCCESFLLTKPVSCKHLIKSHSCPVLTAASCQRPEAYWSDCGTQSAHSDYADEEDS
                     FVSDSSDQVQACGRACFYQSRGFPLVRYAYNLQRVRD"
     exon            666..737
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            738..809
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            810..881
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            882..1097
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1169
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1238
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1310
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            1311..1382
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1451
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            1452..1523
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1589
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1661
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1733
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..1859
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            1860..1975
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2059
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..4648
                     /gene="Lgr4"
                     /gene_synonym="Gpr48"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agaagggaaa aggacgcacg gagccggagc cgccgtgggg agagcgcgga caaccagggt
       61 gtttgtgaga acccgggcgg gggttgggac tccgggccgg catggctgga ggctgcgctc
      121 cgcaaccttg aggagccgtg cggttggagg aggcccggga caggaggagg cggcgatggc
      181 agcgcgcggc ccgggcagtc gctctgggcc gggtcggctg gcctgagcca ctaagccgcg
      241 gggctgcggg tgcgcgtcca tggagcagcg ggaagggaga aactgcggag cgccgcgtcc
      301 taacgctccg gcggcggact gctgaaggaa gccagcgcac gcggacagcc gagggacggc
      361 tcccgggccc cgagccccgc aggccggctg cccctggggc ggcgcggggc gccggagggc
      421 agcggaggcg agccgagaga ggagccgcgg gagaggcagg cgcgggcggc ggccgcagca
      481 atgccgggcc cgctgcggct gctctgcttc ttcgccctgg ggctgctcgg ctcggctggg
      541 cccagcggcg cggcgccgcc tctctgcgct gcgccctgca gctgcgacgg cgaccgtcgg
      601 gtggactgct ccggaaaggg gttgacggcc gtaccggagg gtctcagcgc cttcacccaa
      661 gcactggata tcagtatgaa caatatcacc cagttaccag aagatgcatt taagagtttc
      721 ccatttctag aggagctaca actggctggt aacgaccttt cttttatcca tccaaaagcc
      781 ttgtctgggc tgaaagaact caaagtccta acactccaga ataatcagtt gagaacagtg
      841 cccagtgaag ccattcacgg actgagtgct ttgcagtctt tacgcttaga tgccaaccat
      901 attacctcag tcccggagga cagttttgaa gggcttgtcc agttacgcca tctgtggctg
      961 gatgacaaca gcttgacgga agtgcccgtg cgtcccctca gcaacctgcc aaccctgcag
     1021 gcgctgacct tggctctcaa caacatctca agcatccctg acttcgcttt caccaacctt
     1081 tcaagcttgg tggttctgca tctgcataac aataaaatta aaagcctcag tcaacactgt
     1141 tttgatggac tagataacct ggaaaccttg gacttgaatt acaataactt ggatgagttt
     1201 cctcaggcta ttaaagccct tcccagcctt aaagagctgg gatttcacag taattctatt
     1261 tctgttattc ctgatggagc atttggtggt aatccactgc taagaactat tcatttgtat
     1321 gataatcctc tgtcttttgt ggggaactca gcatttcaca acctgtctga tctgcattcc
     1381 ttagtcattc gtggtgcaag cctggtgcag tggttcccca atctgaccgg aactgtccat
     1441 ttggagagtc taaccttgac agggacaaaa ataagcagca tacctgatga tctgtgccaa
     1501 aaccaaaaga tgctgaggac tctggactta tcttataaca atataagaga ccttccaagt
     1561 tttaatggtt gtcgtgcatt ggaagaaatt tcattgcagc gtaatcaaat ctccctaata
     1621 aaggaaaata cttttcaagg cctaacatct ctaaggattc tagatctgag tagaaacctg
     1681 atccgtgaaa ttcacagtgg agcttttgcg aagcttggga caattactaa cctggatgta
     1741 agtttcaatg aattaacttc atttcctacg gaaggcctaa atgggctcaa tcaactaaag
     1801 cttgtgggta acttcaagct gaaagacgcc ttggcagcca gagactttgc taatctcagg
     1861 tctctatcag taccatatgc ttatcagtgt tgtgcatttt gggggtgtga ctcttatgca
     1921 aatttaaaca cagaagataa cagcccccaa gaacacagtg tgacaaaaga gaaaggtgct
     1981 acagatgcag caaatgtcac cagcactgct gagaacgaag aacatagcca aataattatc
     2041 cactgtacac cttcaacagg tgctttcaag ccctgtgaat atttactggg aagctggatg
     2101 attcgcctta cagtgtggtt cattttcctg gtcgccttgc ttttcaacct gcttgtcatt
     2161 ttaacagtgt ttgcgtcttg ttcatcactg cctgcctcca aactcttcat aggcttgatt
     2221 tctgtgtcta acttactcat gggcatctat actggcatcc ttacttttct tgatgctgtg
     2281 tcctggggcc gatttgccga atttggcatt tggtgggaaa ctggcagcgg ctgcaaggta
     2341 gccgggtctc tggcagtctt ctcctcagag agcgctgtat tcctattaac actggcagct
     2401 gtggaaagaa gcgtatttgc aaaggatttg atgaaacacg ggaagagcag tcacctcaga
     2461 cagttccagg tggccgccct cttagctttg ctgggtgccg cagtggcagg ctgcttcccc
     2521 cttttccacg gagggcaata ttctgcatcg cccttgtgtt tgccgtttcc tacaggagaa
     2581 accccatcgt taggattcac tgtgacctta gtgctattaa actcactggc atttttacta
     2641 atggccatta tctacactaa actatactgc aacttagaga aggaggacct gtcggaaaac
     2701 tcccagtcta gcgtgattaa gcacgttgcc tggctcatct tcacaaactg catcttcttc
     2761 tgccctgttg catttttctc atttgcacca ttgatcacgg caatctccat cagccccgag
     2821 ataatgaagt ctgttacact gatattcttc ccgttgcctg cttgcctgaa tccggtcctg
     2881 tatgttttct tcaacccaaa gtttaaagaa gactggaagc tactgaagcg gcgtgttacc
     2941 aggaaacacg gatctgtttc agtttccatc agcagccaag gcggttgtgg ggaacaggat
     3001 ttctactatg actgtggcat gtattcccac ttgcagggta acctgactgt ctgtgactgc
     3061 tgtgagtcat ttcttttgac aaaaccagta tcatgcaaac acttaataaa atcgcacagt
     3121 tgtcctgtat tgacagcggc ctcttgccag aggccagagg cctactggtc tgattgtggt
     3181 acacagtcag cccattctga ctatgcagat gaagaagatt cctttgtctc agacagctct
     3241 gaccaggtgc aggcctgtgg acgagcctgc ttctaccaga gtcgtggatt ccctctggtg
     3301 cgctatgctt acaatctaca gagagtcaga gactgaactc cagtgtctgc ctgcgactgt
     3361 ttttccccat caaccaaatc acagtgctgg catgggagct atctgtcttt tgacctttca
     3421 tctggaaagc acttgtagaa tcacttcctg gtgtcatgca gaagaggagg aaggtggcag
     3481 tttatttctc agatcagaca tctgtgaaga atgggtgcct aaactataaa ttgttgaaaa
     3541 aaaattgtga tgccccagta atgtgtgctc tattctaaat aaatgcaact tgaaaatggt
     3601 ttgatgtatg gcatagcaat ataaaattag ggttttattt tttttctttc tgatccatca
     3661 ggtgcaaagt tcaaactatt tctgaattaa gcacaaaata aaggacagct gttaatatat
     3721 tttaaatgaa aatgtgaatt tgtaaaacta agaaaatgcc ttgctaattt cacctaatga
     3781 ttcatcatta atctcaggat gactgacttc agggccacaa aagagactgt ctcccagcta
     3841 gagttatgaa gagttcaaac aggcatcccc ttcatgtgtt ttctgtgtcc ataaattaaa
     3901 ggttctgttc aaatgatttg taaatctaaa acctgaagat gttttgagga ctattgacag
     3961 ctgttagatt tttttttaat agcagaccat ttggttttgt tcattgtaca ttgctctggt
     4021 gaaatcatag tgatttatgc tccccctcgg tgttttacaa tcacaatact aggacaaagc
     4081 aaaaggttaa ttgctatgag ggtttagtag aggtgactga agtactaaga aacactgagc
     4141 ttttaatagt atccaagggg tttgatggtt ctaagtaatg tccccatcaa ctggcatgcc
     4201 ctaatgtcac tggctgctct tacatttcca aattcattga cgttatctat cattctgatt
     4261 atataggaag catttcttgt aattgtagga agtcattttc ttgtaattgt aagaagctga
     4321 tagtaatcaa gacaccaagc acttaagttt attttttagt gagctgttgt atgagaactt
     4381 ccatacattt ttcttttttt gcacactgta atttaaaaaa caaaaccatt cagtagctca
     4441 gatgctgtgt gctttggtta aacaagacac aggagtgggg aaatcaaagc ttgtcctatg
     4501 tctttacatt tttaagattt acttgattct tacctatagg cttctgatgt gtttctgtaa
     4561 gaagacttca gactgtgttc agtcagtagc aaacccactg ccatcttagt tctactttaa
     4621 acagaaactt aagctagatt gcagtttg
//
