LOCUS       NM_022226               1907 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus legumain (Lgmn), mRNA.
ACCESSION   NM_022226
VERSION     NM_022226.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Fang,Y., Duan,C., Chen,S., Xie,P., Ai,W., Wang,L., Liu,R. and
            Fang,H.
  TITLE     Increased Legumain/Smad3 expression in atherosclerotic plaque of
            rat thoracic aorta
  JOURNAL   Biomed Pharmacother 119, 109353 (2019)
   PUBMED   31521890
  REMARK    GeneRIF: Legumain and Smad3 expression is highly expressed in
            mainly atherosclerotic plaque macrophages.
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Bai,P., Lyu,L., Yu,T., Zuo,C., Fu,J., He,Y., Wan,Q., Wan,N., Jia,D.
            and Lyu,A.
  TITLE     Macrophage-Derived Legumain Promotes Pulmonary Hypertension by
            Activating the MMP (Matrix Metalloproteinase)-2/TGF (Transforming
            Growth Factor)-beta1 Signaling
  JOURNAL   Arterioscler Thromb Vasc Biol 39 (4), e130-e145 (2019)
   PUBMED   30676070
  REMARK    GeneRIF: Lgmn was upregulated in lung tissues of PAH rats
            challenged with monocrotaline. The synthetic specific Lgmn
            inhibitor RR-11a protects against PAH.
REFERENCE   3  (bases 1 to 1907)
  AUTHORS   Zhang,Z., Song,M., Liu,X., Kang,S.S., Kwon,I.S., Duong,D.M.,
            Seyfried,N.T., Hu,W.T., Liu,Z., Wang,J.Z., Cheng,L., Sun,Y.E.,
            Yu,S.P., Levey,A.I. and Ye,K.
  TITLE     Cleavage of tau by asparagine endopeptidase mediates the
            neurofibrillary pathology in Alzheimer's disease
  JOURNAL   Nat Med 20 (11), 1254-1262 (2014)
   PUBMED   25326800
REFERENCE   4  (bases 1 to 1907)
  AUTHORS   Herskowitz,J.H., Gozal,Y.M., Duong,D.M., Dammer,E.B., Gearing,M.,
            Ye,K., Lah,J.J., Peng,J., Levey,A.I. and Seyfried,N.T.
  TITLE     Asparaginyl endopeptidase cleaves TDP-43 in brain
  JOURNAL   Proteomics 12 (15-16), 2455-2463 (2012)
   PUBMED   22718532
REFERENCE   5  (bases 1 to 1907)
  AUTHORS   Overbye,A., Saetre,F., Hagen,L.K., Johansen,H.T. and Seglen,P.O.
  TITLE     Autophagic activity measured in whole rat hepatocytes as the
            accumulation of a novel BHMT fragment (p10), generated in
            amphisomes by the asparaginyl proteinase, legumain
  JOURNAL   Autophagy 7 (9), 1011-1027 (2011)
   PUBMED   21610319
  REMARK    GeneRIF: Full-length (45 kDa) endogenous BHMT was found to be
            cleaved in an autophagy-dependent (3-methyladenine-sensitive)
            manner in isolated rat hepatocytes to generate a novel N-terminal
            10-kDa fragment (p10), at the cleavage site of legumain.
REFERENCE   6  (bases 1 to 1907)
  AUTHORS   Shirahama-Noda,K., Yamamoto,A., Sugihara,K., Hashimoto,N.,
            Asano,M., Nishimura,M. and Hara-Nishimura,I.
  TITLE     Biosynthetic processing of cathepsins and lysosomal degradation are
            abolished in asparaginyl endopeptidase-deficient mice
  JOURNAL   J Biol Chem 278 (35), 33194-33199 (2003)
   PUBMED   12775715
REFERENCE   7  (bases 1 to 1907)
  AUTHORS   Halfon,S., Patel,S., Vega,F., Zurawski,S. and Zurawski,G.
  TITLE     Autocatalytic activation of human legumain at aspartic acid
            residues
  JOURNAL   FEBS Lett 438 (1-2), 114-118 (1998)
   PUBMED   9821970
REFERENCE   8  (bases 1 to 1907)
  AUTHORS   Chen,J.M., Dando,P.M., Stevens,R.A., Fortunato,M. and Barrett,A.J.
  TITLE     Cloning and expression of mouse legumain, a lysosomal endopeptidase
  JOURNAL   Biochem J 335 (Pt 1) (Pt 1), 111-117 (1998)
   PUBMED   9742219
REFERENCE   9  (bases 1 to 1907)
  AUTHORS   Chen,J.M., Dando,P.M., Rawlings,N.D., Brown,M.A., Young,N.E.,
            Stevens,R.A., Hewitt,E., Watts,C. and Barrett,A.J.
  TITLE     Cloning, isolation, and characterization of mammalian legumain, an
            asparaginyl endopeptidase
  JOURNAL   J Biol Chem 272 (12), 8090-8098 (1997)
   PUBMED   9065484
REFERENCE   10 (bases 1 to 1907)
  AUTHORS   Friedman,J. and Razin,A.
  TITLE     Studies on the biological role of DNA methylation. II. Role of
            phiX174 DNA methylation in the process of viral progeny DNA
            synthesis
  JOURNAL   Nucleic Acids Res 3 (10), 2665-2675 (1976)
   PUBMED   136644
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            On Nov 30, 2020 this sequence version replaced NM_022226.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643292.27077.1,
                                           SRR26643284.9232.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760433 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-86                JAXUCZ010000006.1  127347243-127347328 c
            87-259              JAXUCZ010000006.1  127334706-127334878 c
            260-357             JAXUCZ010000006.1  127321919-127322016 c
            358-439             JAXUCZ010000006.1  127321090-127321171 c
            440-525             JAXUCZ010000006.1  127319504-127319589 c
            526-601             JAXUCZ010000006.1  127318652-127318727 c
            602-664             JAXUCZ010000006.1  127317814-127317876 c
            665-731             JAXUCZ010000006.1  127317054-127317120 c
            732-850             JAXUCZ010000006.1  127315585-127315703 c
            851-940             JAXUCZ010000006.1  127315402-127315491 c
            941-1141            JAXUCZ010000006.1  127313640-127313840 c
            1142-1312           JAXUCZ010000006.1  127310446-127310616 c
            1313-1380           JAXUCZ010000006.1  127309690-127309757 c
            1381-1907           JAXUCZ010000006.1  127308913-127309439 c
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q32"
     gene            1..1907
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /note="legumain"
                     /db_xref="GeneID:63865"
                     /db_xref="RGD:619832"
     exon            1..86
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            87..259
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..1423
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /EC_number="3.4.22.34"
                     /function="cysteine endopeptidase"
                     /function="asparagine-specific endoprotease"
                     /note="protease, cysteine, 1 (legumain); protease,
                     cysteine 1; asparaginyl endopeptidase"
                     /codon_start=1
                     /product="legumain precursor"
                     /protein_id="NP_071562.3"
                     /db_xref="GeneID:63865"
                     /db_xref="RGD:619832"
                     /translation="MIWKVAVLLSLVLGAGAVHIGVDDPEDGGKHWVVIVAGSNGWYN
                     YRHQADACHAYQIIHRNGIPDEQIIVMMYDDIANNEENPTPGVVINRPNGTDVYKGVP
                     KDYTGEDVTPENFLAVLRGDEEAVKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPN
                     EDLHVKDLNKTIRYMYEHKMYQKMVFYIEACESGSMMNHLPDDIDVYATTAANPNESS
                     YACYYDEERSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKS
                     ISTMKVMQFQGMKHRASSPISLPPVTHLDLTPSPDVPLTILKRKLLRTNNMKESQVLV
                     GQIQHLLDARHIIEKSVQKIVSLLAGFGETAQKHLSERAMLTAHDCHQEAVTHFRTHC
                     FNWHSVMYEHALRYLYVLANLCEKPYPIDRIKMAMDKVCLSHY"
     exon            260..357
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            358..439
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            440..525
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            526..601
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            602..664
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            665..731
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            732..850
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            851..940
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1141
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1312
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1380
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1381..1907
                     /gene="Lgmn"
                     /gene_synonym="Prsc1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcggactcta gaggtggctg ttctgagtct gcgcgacgca cggaattccc acggttctgc
       61 agtcaccgcg gcgatcaccc agccagtctt ctgaagcgga cactgtgggt gcagaatgat
      121 ctggaaagtg gctgtgcttc tcagcctagt gctgggtgct ggtgctgttc acatcggtgt
      181 ggatgaccct gaggacggtg gcaagcactg ggtggtgatt gtggcgggct ccaatggctg
      241 gtataattac cgacaccagg cagacgcatg ccacgcctac cagatcatcc accggaacgg
      301 gattcctgac gagcagatca tagtgatgat gtatgacgac atcgccaaca atgaagaaaa
      361 cccgactcca ggtgttgtga tcaaccgacc taacggcaca gacgtgtaca agggagtccc
      421 gaaggactac actggagagg atgtgactcc agagaatttc ctcgcagtgc tgagaggcga
      481 cgaagaagct gtgaagggca aagggtccgg aaaagtcttg aagagtgggc cccgggatca
      541 tgtatttgtt tacttcaccg accacggagc cactggaatc ctggtgtttc ctaacgaaga
      601 tcttcatgtc aaggacctga ataagactat tcgctacatg tatgagcaca aaatgtacca
      661 gaagatggtg ttttacattg aagcatgtga gtccggctcc atgatgaacc acctgcccga
      721 tgacatcgat gtttatgcaa ccactgccgc caaccccaac gagtcatctt acgcctgcta
      781 ctatgacgag gagaggagca cttacctggg tgactggtac agcgtcaact ggatggaaga
      841 ttccgatgtg gaggatctga ccaaagagac acttcacaag cagtaccacc tggtcaagtc
      901 ccacaccaac accagtcacg tcatgcagta tgggaacaaa tctatctcta ccatgaaagt
      961 gatgcagttt caaggaatga agcacagagc cagttcaccc atctcgctgc ctccagtcac
     1021 acaccttgac ctcaccccca gccctgacgt gcccctgacc atcctgaaaa ggaagctgct
     1081 gagaaccaac aacatgaagg aatcccaagt tctcgtcggg cagatccagc accttctgga
     1141 tgccaggcac atcattgaga agtctgtgca aaagatcgtt tccctgctgg caggatttgg
     1201 ggaaactgct cagaaacatc tgtcagagag agccatgctc acagcacatg actgccatca
     1261 ggaggccgtg acccacttcc gcacacactg ctttaactgg cactcagtca tgtacgagca
     1321 tgccttgcgg tatttgtacg tgctggccaa cctctgtgag aagccatatc cgattgacag
     1381 gataaagatg gccatggaca aagtgtgcct tagtcactac tgaacagctc tcttcccagt
     1441 gcttctagaa gagtgagcac agtacactgg aatgtgaacc aaccggagtg aaccaagcgg
     1501 agaccggaag ggcggagtca gaggcagcac ctaagccccg cccccaggga taccgtccgc
     1561 ccaccccagg gcttgctttc tgaggatacc tgcttactaa gaagccagtt tgggtctgga
     1621 ggaaggaact tttgcttctt aggaattttt gggtttgttt tgttgtccat tagctttcaa
     1681 gagcagattc actgcggatt ctgtagccag tgaaggaacc ggagaaattc tcgaagctga
     1741 aacctcttgt cgccttcaca gtgattttac cgaagagggc aaaagcaagt ccctactgga
     1801 gaattatttt tagaatatat aatttttgat tgcttttata ttttattctg taataatgga
     1861 tgtcctaaaa caaataagtg aagtgagaca tttgttcctg gactctg
//
