LOCUS       NM_001005884            2589 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Rattus norvegicus leucine zipper and EF-hand containing
            transmembrane protein 1 (Letm1), mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_001005884 XM_223541
VERSION     NM_001005884.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Shao,J., Fu,Z., Ji,Y., Guan,X., Guo,S., Ding,Z., Yang,X., Cong,Y.
            and Shen,Y.
  TITLE     Leucine zipper-EF-hand containing transmembrane protein 1 (LETM1)
            forms a Ca2+/H+ antiporter
  JOURNAL   Sci Rep 6, 34174 (2016)
   PUBMED   27669901
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Hou,T., Jian,C., Xu,J., Huang,A.Y., Xi,J., Hu,K., Wei,L., Cheng,H.
            and Wang,X.
  TITLE     Identification of EFHD1 as a novel Ca(2+) sensor for mitoflash
            activation
  JOURNAL   Cell Calcium 59 (5), 262-270 (2016)
   PUBMED   26975899
REFERENCE   3  (bases 1 to 2589)
  AUTHORS   Doonan,P.J., Chandramoorthy,H.C., Hoffman,N.E., Zhang,X.,
            Cardenas,C., Shanmughapriya,S., Rajan,S., Vallem,S., Chen,X.,
            Foskett,J.K., Cheung,J.Y., Houser,S.R. and Madesh,M.
  TITLE     LETM1-dependent mitochondrial Ca2+ flux modulates cellular
            bioenergetics and proliferation
  JOURNAL   FASEB J 28 (11), 4936-4949 (2014)
   PUBMED   25077561
  REMARK    GeneRIF: Reconstitution of LETM1 or antioxidant overexpression
            rescued mitochondrial Ca(2+) transport and bioenergetics
            Erratum:[FASEB J. 2015 Aug;29(8):3593. doi:
            10.1096/fj.14-256453ERR.. PMID: 26232296]
REFERENCE   4  (bases 1 to 2589)
  AUTHORS   Zhang,X., Chen,G., Lu,Y., Liu,J., Fang,M., Luo,J., Cao,Q. and
            Wang,X.
  TITLE     Association of mitochondrial letm1 with epileptic seizures
  JOURNAL   Cereb Cortex 24 (10), 2533-2540 (2014)
   PUBMED   23645710
  REMARK    GeneRIF: Letm1 expression is decreased in patients with intractable
            TLE and a rat model of epilepsy. Down-regulation of Letm1 leads to
            increases mitochondrial swelling and decreased MT-CYB expression,
            which is associated with susceptibility to seizures.
REFERENCE   5  (bases 1 to 2589)
  AUTHORS   Jiang,D., Zhao,L. and Clapham,D.E.
  TITLE     Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+
            antiporter
  JOURNAL   Science 326 (5949), 144-147 (2009)
   PUBMED   19797662
REFERENCE   6  (bases 1 to 2589)
  AUTHORS   Tamai,S., Iida,H., Yokota,S., Sayano,T., Kiguchiya,S., Ishihara,N.,
            Hayashi,J., Mihara,K. and Oka,T.
  TITLE     Characterization of the mitochondrial protein LETM1, which
            maintains the mitochondrial tubular shapes and interacts with the
            AAA-ATPase BCS1L
  JOURNAL   J Cell Sci 121 (Pt 15), 2588-2600 (2008)
   PUBMED   18628306
REFERENCE   7  (bases 1 to 2589)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   8  (bases 1 to 2589)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   9  (bases 1 to 2589)
  AUTHORS   Endele,S., Fuhry,M., Pak,S.J., Zabel,B.U. and Winterpacht,A.
  TITLE     LETM1, a novel gene encoding a putative EF-hand Ca(2+)-binding
            protein, flanks the Wolf-Hirschhorn syndrome (WHS) critical region
            and is deleted in most WHS patients
  JOURNAL   Genomics 60 (2), 218-225 (1999)
   PUBMED   10486213
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC083642.1.
            
            On Oct 23, 2004 this sequence version replaced XM_223541.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC083642.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14q21"
     gene            1..2589
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /note="leucine zipper and EF-hand containing transmembrane
                     protein 1"
                     /db_xref="GeneID:305457"
                     /db_xref="RGD:1359678"
     exon            1..278
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..2419
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /note="LETM1 and EF-hand domain-containing protein 1,
                     mitochondrial; leucine zipper-EF-hand-containing
                     transmembrane protein 1; mitochondrial proton/calcium
                     exchanger protein; electroneutral mitochondrial
                     K(+)/H(+)exchanger"
                     /codon_start=1
                     /product="mitochondrial proton/calcium exchanger protein
                     precursor"
                     /protein_id="NP_001005884.1"
                     /db_xref="GeneID:305457"
                     /db_xref="RGD:1359678"
                     /translation="MASILLRSCRGRGPARLASPRAASPRGSLRDRACLSCTRTVGLT
                     SHESVLSRCCTPANPVYLYFKSEPLSCWTQRPECQGTVARAAWTPASARLVVTGPQYL
                     PVRGWHSSSPLGEDSMIEKSLKSLKDKNKKLEEGGPVYSPPAQVVVKKSLGQKILDEL
                     KHYYHGFRLLWIDTKIAARMLWRILNGHTLTRRERRQFLRICADLFRLVPFLVFVVVP
                     FMEFLLPVVVKLFPNMLPSTFETQSIKEERLKKELRVKLELAKFLQDTIEEMALKNKA
                     AKGNATKDFSAFFQKIRETGERPSNEEIMRFSKLFEDELTLDNLTRPQLVALCKLLEL
                     QSIGTNNFLRFQLTMRLRSIKADDKLISEEGVDSLTVKELQAACRARGMRALGVTEDR
                     LKGQLKQWLDLHLYHEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVK
                     AAEVEGEQVDNKAKLEATLQEEAAIQQEHLEELKRAAETAKDIQPEVAEATVPGRPGA
                     ELQPKMVDVIPPSEILKDTAPVLEGLKGEEITKEEIDILSDACSKLKEQKKSLTKEKE
                     ELELLKEDVQDYSEDLQEIKKELSKTGDEKYIEESTASKRLSKRVQQMIGQIDGLITQ
                     LETTQQNGKLDPAAASSPTGESVISVDELISAMKQIKHIPEHKLISLTSALDENKDGN
                     INIDDLVKVIDLVNKEDVQISTTQVAEIVATLEKEEKVEEKEKAKEKAEKEAAEVKN"
     exon            279..339
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /inference="alignment:Splign:2.1.0"
     exon            340..790
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /inference="alignment:Splign:2.1.0"
     exon            791..934
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1072
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1276
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1396
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1528
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1669
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /inference="alignment:Splign:2.1.0"
     exon            1670..1804
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1939
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /inference="alignment:Splign:2.1.0"
     exon            1940..2130
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2269
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /inference="alignment:Splign:2.1.0"
     exon            2270..2565
                     /gene="Letm1"
                     /gene_synonym="KHE"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cccggcctgt cccgccctgc gcctccgaac gtctgtgccg ggacatggcc gctgctgatg
       61 ccgggtcctt gagctgagcg cctactgccg gagccaacct ctgcggtcaa ccgtccgcac
      121 gatctgggcc caggagccgg gagggcgaaa atcagccgag gaagctgagg ctgccatagg
      181 acgccgccgc ggcgggcgca tggcgtccat cttgctcagg agctgccgcg gccgggggcc
      241 tgcccgcctc gcgtcgcccc gggccgcttc cccgcggggt agtctgaggg atcgagcttg
      301 tctcagttgt accaggaccg tggggttgac gagccatgag agtgttctgt cccgctgttg
      361 tactccagcc aaccctgtgt acctctactt caaaagtgag cccctcagct gttggactca
      421 gaggcctgag tgccagggca ccgtggcaag ggcagcatgg acacctgcct ctgcgaggtt
      481 ggttgttacg ggacctcagt accttcctgt gcgtggctgg cactcatcat ccccactagg
      541 agaggactct atgatagaga aatcccttaa gtctttaaag gacaagaata agaagctgga
      601 agaaggtggc cccgtgtaca gcccgccagc tcaggtggta gtgaagaagt ccctggggca
      661 gaagatactg gatgagctga agcactacta ccatggcttc cgcctgctgt ggatcgacac
      721 caagatagcc gcccgtatgc tgtggcgcat cctgaatggc catacgctga cccgccggga
      781 gcgcaggcag tttctccgga tttgtgcaga cctcttccgt ctagtaccct tcctggtgtt
      841 cgtggtggta cccttcatgg aattcctgct gcctgtagtt gtgaagctct tccccaatat
      901 gctgccctcc acatttgaga cccagtctat caaggaggaa aggctgaaga aggagcttag
      961 ggtgaagctg gagctagcca agtttctcca agacaccata gaggaaatgg ccctgaagaa
     1021 caaggcagcc aaagggaatg ccaccaagga tttctctgcc tttttccaga agatccggga
     1081 gacaggggag agacccagca atgaagaaat catgcgtttt tccaaattat ttgaggatga
     1141 actgaccctg gataacctaa cgaggcctca actggtagca ctgtgcaagc tgctggagct
     1201 tcagtccatt ggtaccaaca acttcttgcg tttccagctc accatgcggc tgaggtctat
     1261 aaaggctgat gataagctga tttctgagga aggggtagac agtctgactg tgaaggaatt
     1321 gcaggcagcg tgtcgagcac ggggcatgcg agcacttggt gtcacagaag accgtctgaa
     1381 gggccagctg aaacagtggc tggacttgca cctgtatcac gagatcccta catcattgct
     1441 catactgtcc cgggccatgt acctcccaga caccctctca cctgccgacc agctgaagtc
     1501 caccttgcag acccttccag aaattgtggc aaaggaggct caggtaaaag cggctgaggt
     1561 ggagggcgaa caggtagaca acaaggcgaa gctggaggcc acactgcagg aagaggccgc
     1621 catccagcag gagcacctgg aagaactaaa gagagccgct gagactgcga aggacatcca
     1681 gccagaagta gcagaagcca ctgtccctgg gaggccaggc gccgagcttc agcccaagat
     1741 ggttgatgtg atcccgccct cagagatact aaaggataca gcacctgttc tggaaggttt
     1801 gaagggagaa gagataacca aggaggaaat tgacatcctc agtgacgcct gctccaagct
     1861 aaaggagcag aagaagtccc tgaccaagga gaaggaggag ctggagctgc tgaaagagga
     1921 tgtccaggac tacagtgagg acttgcagga gatcaagaag gaactttcca agacgggtga
     1981 tgagaaatac atagaagagt ctacagccag caagaggctg tccaagcggg tgcagcagat
     2041 gatcgggcag atcgatggcc tcatcacaca gctggagacc acgcaacaga acggcaagct
     2101 ggaccccgct gctgcgagct ctcccacagg ggagagtgtc atcagcgtcg acgagctcat
     2161 cagcgccatg aagcaaatca agcacattcc agaacacaag ctgatcagtt tgacctcagc
     2221 cctggatgaa aataaggatg gcaatatcaa catcgatgat cttgttaagg taatcgactt
     2281 ggtgaacaaa gaagatgtcc agatctctac cacccaggta gccgagattg tggccacact
     2341 agagaaggaa gaaaaggtgg aagagaagga aaaggccaag gaaaaggctg agaaggaggc
     2401 ggcagaagtg aagaactaga gctgccgggc tcttggcccc acggggcctg ccttcatgtc
     2461 atacccacca tggtgacacg gcacacgagt gattgctctg aggtgatttt tagtggcaaa
     2521 tctaatattt tgtctgtgga ataaatcaga aacttccaca atgaaaaaaa aaaaaaaaaa
     2581 aaaaaaaaa
//
