LOCUS       NM_012595               1345 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus lactate dehydrogenase B (Ldhb), transcript
            variant 1, mRNA.
ACCESSION   NM_012595
VERSION     NM_012595.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Feng,H., Wu,J., Chen,P., Wang,J., Deng,Y., Zhu,G., Xian,J.,
            Huang,L. and Ouyang,W.
  TITLE     MicroRNA-375-3p inhibitor suppresses angiotensin II-induced
            cardiomyocyte hypertrophy by promoting lactate dehydrogenase B
            expression
  JOURNAL   J Cell Physiol 234 (8), 14198-14209 (2019)
   PUBMED   30618075
  REMARK    GeneRIF: miR-375-3p inhibitor suppresses Ang II-induced
            cardiomyocyte hypertrophy by promoting LDHB expression.
REFERENCE   3  (bases 1 to 1345)
  AUTHORS   Wei,X., Das,J., Fragoza,R., Liang,J., Bastos de Oliveira,F.M.,
            Lee,H.R., Wang,X., Mort,M., Stenson,P.D., Cooper,D.N., Lipkin,S.M.,
            Smolka,M.B. and Yu,H.
  TITLE     A massively parallel pipeline to clone DNA variants and examine
            molecular phenotypes of human disease mutations
  JOURNAL   PLoS Genet 10 (12), e1004819 (2014)
   PUBMED   25502805
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1345)
  AUTHORS   Stiebler,A.C., Freitag,J., Schink,K.O., Stehlik,T., Tillmann,B.A.,
            Ast,J. and Bolker,M.
  TITLE     Ribosomal readthrough at a short UGA stop codon context triggers
            dual localization of metabolic enzymes in Fungi and animals
  JOURNAL   PLoS Genet 10 (10), e1004685 (2014)
   PUBMED   25340584
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1345)
  AUTHORS   Schueren,F., Lingner,T., George,R., Hofhuis,J., Dickel,C.,
            Gartner,J. and Thoms,S.
  TITLE     Peroxisomal lactate dehydrogenase is generated by translational
            readthrough in mammals
  JOURNAL   Elife 3, e03640 (2014)
   PUBMED   25247702
  REMARK    GeneRIF: The readthrough-extended lactate dehydrogenase subunit
            LDHBx can also co-import LDHA, the other LDH subunit, into
            peroxisomes.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1345)
  AUTHORS   Beier,H. and Grimm,M.
  TITLE     Misreading of termination codons in eukaryotes by natural nonsense
            suppressor tRNAs
  JOURNAL   Nucleic Acids Res 29 (23), 4767-4782 (2001)
   PUBMED   11726686
  REMARK    Review article
REFERENCE   7  (bases 1 to 1345)
  AUTHORS   Tsuji,S., Qureshi,M.A., Hou,E.W., Fitch,W.M. and Li,S.S.
  TITLE     Evolutionary relationships of lactate dehydrogenases (LDHs) from
            mammals, birds, an amphibian, fish, barley, and bacteria: LDH cDNA
            sequences from Xenopus, pig, and rat
  JOURNAL   Proc Natl Acad Sci U S A 91 (20), 9392-9396 (1994)
   PUBMED   7937776
REFERENCE   8  (bases 1 to 1345)
  AUTHORS   LeVan,K.M. and Goldberg,E.
  TITLE     Properties of human testis-specific lactate dehydrogenase expressed
            from Escherichia coli
  JOURNAL   Biochem J 273 (Pt 3) (Pt 3), 587-592 (1991)
   PUBMED   1996957
REFERENCE   9  (bases 1 to 1345)
  AUTHORS   Yasue,M., Serikawa,T. and Yamada,J.
  TITLE     Chromosomal assignments of 23 biochemical loci of the rat by using
            rat x mouse somatic cell hybrids
  JOURNAL   Cytogenet Cell Genet 57 (2-3), 142-148 (1991)
   PUBMED   1914521
REFERENCE   10 (bases 1 to 1345)
  AUTHORS   Beebee,T.J. and Carty,D.S.
  TITLE     Purification and radioimmunoassay of rat lactate dehydrogenase A
            and B subunits
  JOURNAL   Biochem J 205 (2), 313-320 (1982)
   PUBMED   7138505
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from FM054345.1, U07181.1 and
            BU759234.1.
            
            On Oct 14, 2015 this sequence version replaced NM_012595.1.
            
            Summary: This gene encodes the B subunit of lactate dehydrogenase
            enzyme, which catalyzes the interconversion of pyruvate and lactate
            with concomitant interconversion of NADH and NAD+ in a
            post-glycolysis process. Alternatively spliced transcript variants
            have also been found for this gene. Recent studies have shown that
            a C-terminally extended isoform is produced by use of an
            alternative in-frame translation termination codon via a stop codon
            readthrough mechanism, and that this isoform is localized in the
            peroxisomes. Pseudogenes have been identified on chromosomes X and
            9. [provided by RefSeq, Feb 2016].
            
            Transcript Variant: This variant (1) encodes two isoforms, which
            result from the use of alternative in-frame translation termination
            codons. The shorter isoform (Ldhb) results from translation
            termination at the upstream UGA stop codon, while the longer
            isoform (Ldhbx) results from UGA stop codon readthrough to the
            downstream UAG termination codon. This RefSeq represents the
            shorter isoform (Ldhb), which is localized in the cytosol. Variants
            1 and 2 encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ212899.1, BC059149.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN06621351, SAMN11044071
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-108               FM054345.1         1-108
            109-1325            U07181.1           1-1217
            1326-1345           BU759234.1         1-20                c
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q44"
     gene            1..1345
                     /gene="Ldhb"
                     /gene_synonym="Ldh2"
                     /note="lactate dehydrogenase B"
                     /db_xref="GeneID:24534"
                     /db_xref="RGD:2997"
     exon            1..127
                     /gene="Ldhb"
                     /gene_synonym="Ldh2"
                     /inference="alignment:Splign:2.1.0"
     exon            128..262
                     /gene="Ldhb"
                     /gene_synonym="Ldh2"
                     /inference="alignment:Splign:2.1.0"
     CDS             134..1138
                     /gene="Ldhb"
                     /gene_synonym="Ldh2"
                     /EC_number="1.1.1.27"
                     /note="isoform Ldhb is encoded by transcript variant 1;
                     L-lactate dehydrogenase B chain; LDH-B; LDH-H; LDH heart
                     subunit; Lactate dehydrogenease B"
                     /codon_start=1
                     /product="L-lactate dehydrogenase B chain isoform Ldhb"
                     /protein_id="NP_036727.1"
                     /db_xref="GeneID:24534"
                     /db_xref="RGD:2997"
                     /translation="MATLKEKLIAPVADDETAVPNNKITVVGVGQVGMACAISILGKS
                     LADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQ
                     EGESRLNLVQRNVNVFKFIIPQIVKYSPDCTIIVVSNPVDILTYVTWKLSGLPKHRVI
                     GSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPE
                     MGTDNDSENWKEVHKMVVDSAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVST
                     MVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLRKSADTLWDIQKDLKDL
                     "
     exon            263..380
                     /gene="Ldhb"
                     /gene_synonym="Ldh2"
                     /inference="alignment:Splign:2.1.0"
     exon            381..554
                     /gene="Ldhb"
                     /gene_synonym="Ldh2"
                     /inference="alignment:Splign:2.1.0"
     exon            555..728
                     /gene="Ldhb"
                     /gene_synonym="Ldh2"
                     /inference="alignment:Splign:2.1.0"
     exon            729..846
                     /gene="Ldhb"
                     /gene_synonym="Ldh2"
                     /inference="alignment:Splign:2.1.0"
     exon            847..970
                     /gene="Ldhb"
                     /gene_synonym="Ldh2"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1330
                     /gene="Ldhb"
                     /gene_synonym="Ldh2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1306..1311
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ldhb"
                     /gene_synonym="Ldh2"
     polyA_site      1330
                     /gene="Ldhb"
                     /gene_synonym="Ldh2"
ORIGIN      
        1 cagggcggca gccccgcctc ctcctcctcc tcttcttgta gagccagagt cggctgtccg
       61 cagagcagcc tgctgacttt gcagtggttc ccctgcctca gcgcgccgca gagcctcctc
      121 ttgtctggac aagatggcaa cccttaagga aaagctcatt gcgccagtcg cagacgacga
      181 gactgccgtc ccgaacaaca agattactgt agtaggcgtt ggacaagttg gaatggcttg
      241 tgctatcagc attctgggga agtctctggc tgatgagctt gccctggtgg atgtcttgga
      301 agacaagctc aaaggagaaa tgatggatct gcagcacggg agcttatttc tccagactcc
      361 gaaaatcgtg gctgataaag attactccgt gacagccaat tctaagattg tggtggtgac
      421 cgcgggagtc cgccagcagg agggggagag tcggctcaac ctggtgcaga gaaacgtcaa
      481 tgtattcaag ttcattattc ctcagatcgt caagtacagc cccgactgca ccatcatcgt
      541 ggtttccaac ccagtggata ttcttaccta tgtcacctgg aagctgagcg ggctacctaa
      601 gcaccgcgtg attggaagtg gatgcaatct ggattctgct cggtttcgtt acctcatggc
      661 cgaaaagctt ggtattcatc ccagcagctg ccacggctgg atcctgggcg agcacgggga
      721 ctccagtgtg gcagtgtgga gcggggtgaa tgtggcagga gtctccctcc aggaactgaa
      781 cccagagatg ggaacggaca atgacagcga gaactggaag gaggtgcata agatggtggt
      841 ggacagtgcc tatgaagtca tcaagctaaa aggctacacc aactgggcca tcggcctaag
      901 tgtggctgac ctcatcgaat ccatgctgaa aaacctctct cggattcacc ccgtgtctac
      961 aatggtgaag ggaatgtacg gcatcgagaa cgaagtcttc ctcagtctcc cgtgcatcct
     1021 taatgctcgg ggactgacca gcgtcatcaa ccagaagctg aaggacgatg aggtcgctca
     1081 gctcaggaag agtgcggaca ccctgtggga tatccagaaa gacctcaagg acctgtgact
     1141 gccaggcgcc aggctgtaga aatccaaacc tccaatgtga ctaagtgaac ctttagtctt
     1201 cggccttgta cgtaggtcac agtttgcttc ttccctaaca tgtgataatg agctcacaga
     1261 tcaaaaccag gagtgtttga tgtttgcact aggagctcct gaacaaataa agtttagcaa
     1321 ttgcagcata aaaaaaaaaa aaaaa
//
