LOCUS       NM_001100709            2170 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Rattus norvegicus LCK proto-oncogene, Src family tyrosine kinase
            (Lck), transcript variant 2, mRNA.
ACCESSION   NM_001100709 XM_001061667 XM_001061773 XM_001062229 XM_232763
VERSION     NM_001100709.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Xuan,J., Zhang,J., He,J., Zhao,K., Miao,S., Chen,G. and Wei,S.
  TITLE     RNA-Sequencing Analysis and Expression of Lymphocyte-Specific
            Protein Tyrosine Kinase, Linker for Activation of T Cells, and
            70-kDa T-Cell Receptor Zeta-Chain Associated Protein Kinase in Rat
            Liver Transplant Rejection
  JOURNAL   Exp Clin Transplant 21 (12), 961-972 (2023)
   PUBMED   38263783
  REMARK    GeneRIF: RNA-Sequencing Analysis and Expression of
            Lymphocyte-Specific Protein Tyrosine Kinase, Linker for Activation
            of T Cells, and 70-kDa T-Cell Receptor Zeta-Chain Associated
            Protein Kinase in Rat Liver Transplant Rejection.
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Ono,A., Hirooka,K., Nakano,Y., Nitta,E., Nishiyama,A. and
            Tsujikawa,A.
  TITLE     Gene expression changes in the retina after systemic administration
            of aldosterone
  JOURNAL   Jpn J Ophthalmol 62 (4), 499-507 (2018)
   PUBMED   29713904
REFERENCE   3  (bases 1 to 2170)
  AUTHORS   Kochl,R., Thelen,F., Vanes,L., Brazao,T.F., Fountain,K., Xie,J.,
            Huang,C.L., Lyck,R., Stein,J.V. and Tybulewicz,V.L.
  TITLE     WNK1 kinase balances T cell adhesion versus migration in vivo
  JOURNAL   Nat Immunol 17 (9), 1075-1083 (2016)
   PUBMED   27400149
  REMARK    Erratum:[Nat Immunol. 2017 Jan 19;18(2):246. doi:
            10.1038/ni0217-246a.. PMID: 28102214]
REFERENCE   4  (bases 1 to 2170)
  AUTHORS   Li,J.P., Yang,C.Y., Chuang,H.C., Lan,J.L., Chen,D.Y., Chen,Y.M.,
            Wang,X., Chen,A.J., Belmont,J.W. and Tan,T.H.
  TITLE     The phosphatase JKAP/DUSP22 inhibits T-cell receptor signalling and
            autoimmunity by inactivating Lck
  JOURNAL   Nat Commun 5, 3618 (2014)
   PUBMED   24714587
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2170)
  AUTHORS   Hui,E. and Vale,R.D.
  TITLE     In vitro membrane reconstitution of the T-cell receptor proximal
            signaling network
  JOURNAL   Nat Struct Mol Biol 21 (2), 133-142 (2014)
   PUBMED   24463463
REFERENCE   6  (bases 1 to 2170)
  AUTHORS   Alai,M., Mui,A.L., Cutler,R.L., Bustelo,X.R., Barbacid,M. and
            Krystal,G.
  TITLE     Steel factor stimulates the tyrosine phosphorylation of the
            proto-oncogene product, p95vav, in human hemopoietic cells
  JOURNAL   J Biol Chem 267 (25), 18021-18025 (1992)
   PUBMED   1381360
REFERENCE   7  (bases 1 to 2170)
  AUTHORS   Straus,D.B. and Weiss,A.
  TITLE     Genetic evidence for the involvement of the lck tyrosine kinase in
            signal transduction through the T cell antigen receptor
  JOURNAL   Cell 70 (4), 585-593 (1992)
   PUBMED   1505025
REFERENCE   8  (bases 1 to 2170)
  AUTHORS   Molina,T.J., Kishihara,K., Siderovski,D.P., van Ewijk,W.,
            Narendran,A., Timms,E., Wakeham,A., Paige,C.J., Hartmann,K.U.,
            Veillette,A. et al.
  TITLE     Profound block in thymocyte development in mice lacking p56lck
  JOURNAL   Nature 357 (6374), 161-164 (1992)
   PUBMED   1579166
REFERENCE   9  (bases 1 to 2170)
  AUTHORS   Barber,E.K., Dasgupta,J.D., Schlossman,S.F., Trevillyan,J.M. and
            Rudd,C.E.
  TITLE     The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase
            (p56lck) that phosphorylates the CD3 complex
  JOURNAL   Proc Natl Acad Sci U S A 86 (9), 3277-3281 (1989)
   PUBMED   2470098
REFERENCE   10 (bases 1 to 2170)
  AUTHORS   Levinson,A.D., Oppermann,H., Levintow,L., Varmus,H.E. and
            Bishop,J.M.
  TITLE     Evidence that the transforming gene of avian sarcoma virus encodes
            a protein kinase associated with a phosphoprotein
  JOURNAL   Cell 15 (2), 561-572 (1978)
   PUBMED   214242
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Jan 4, 2023 this sequence version replaced NM_001100709.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC160881.1, SRR26643284.7729.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-324               JAXUCZ010000005.1  147183327-147183650 c
            325-406             JAXUCZ010000005.1  147182532-147182613 c
            407-497             JAXUCZ010000005.1  147182268-147182358 c
            498-596             JAXUCZ010000005.1  147181904-147182002 c
            597-700             JAXUCZ010000005.1  147181505-147181608 c
            701-850             JAXUCZ010000005.1  147181148-147181297 c
            851-1003            JAXUCZ010000005.1  147180719-147180871 c
            1004-1183           JAXUCZ010000005.1  147180401-147180580 c
            1184-1260           JAXUCZ010000005.1  147178276-147178352 c
            1261-1414           JAXUCZ010000005.1  147178041-147178194 c
            1415-1546           JAXUCZ010000005.1  147177829-147177960 c
            1547-2170           JAXUCZ010000005.1  147172642-147173265 c
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..2170
                     /gene="Lck"
                     /gene_synonym="Lck1; Lcktkr; p56-LCK"
                     /note="LCK proto-oncogene, Src family tyrosine kinase"
                     /db_xref="GeneID:313050"
                     /db_xref="RGD:2994"
     exon            1..324
                     /gene="Lck"
                     /gene_synonym="Lck1; Lcktkr; p56-LCK"
                     /inference="alignment:Splign:2.1.0"
     CDS             220..1749
                     /gene="Lck"
                     /gene_synonym="Lck1; Lcktkr; p56-LCK"
                     /EC_number="2.7.10.2"
                     /note="lymphocyte-specific protein tyrosine kinase;
                     lymphocyte cell-specific protein-tyrosine kinase;
                     lymphocyte protein tyrosine kinase"
                     /codon_start=1
                     /product="proto-oncogene tyrosine-protein kinase LCK"
                     /protein_id="NP_001094179.1"
                     /db_xref="GeneID:313050"
                     /db_xref="RGD:2994"
                     /translation="MGCVCSSNPEDDWMENIDVCENCHYPIVPLDSKSTLPIRTGSEV
                     RDPLVTYEGSLPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQS
                     LTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTA
                     GSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHDLVRHYTNASDGLCT
                     KLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLK
                     QGSMSPDAFLAEANLMKQLQHPRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGI
                     KLNVNKLLDMAAQIAEGMAFIEEQNYIHRDLRAANILVSDTLSCKIADFGLARLIEDN
                     EYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVI
                     QNLEKGYRMVRPDNCPEELYHLMMLCWKERPEDRPTFDYLRSVLDDFFTATEGQYQPQ
                     P"
     exon            325..406
                     /gene="Lck"
                     /gene_synonym="Lck1; Lcktkr; p56-LCK"
                     /inference="alignment:Splign:2.1.0"
     exon            407..497
                     /gene="Lck"
                     /gene_synonym="Lck1; Lcktkr; p56-LCK"
                     /inference="alignment:Splign:2.1.0"
     exon            498..596
                     /gene="Lck"
                     /gene_synonym="Lck1; Lcktkr; p56-LCK"
                     /inference="alignment:Splign:2.1.0"
     exon            597..700
                     /gene="Lck"
                     /gene_synonym="Lck1; Lcktkr; p56-LCK"
                     /inference="alignment:Splign:2.1.0"
     exon            701..850
                     /gene="Lck"
                     /gene_synonym="Lck1; Lcktkr; p56-LCK"
                     /inference="alignment:Splign:2.1.0"
     exon            851..1003
                     /gene="Lck"
                     /gene_synonym="Lck1; Lcktkr; p56-LCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1183
                     /gene="Lck"
                     /gene_synonym="Lck1; Lcktkr; p56-LCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1260
                     /gene="Lck"
                     /gene_synonym="Lck1; Lcktkr; p56-LCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1414
                     /gene="Lck"
                     /gene_synonym="Lck1; Lcktkr; p56-LCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..1546
                     /gene="Lck"
                     /gene_synonym="Lck1; Lcktkr; p56-LCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..2170
                     /gene="Lck"
                     /gene_synonym="Lck1; Lcktkr; p56-LCK"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagcctcctg tgaactcgtt gcttgagggc tcggagtaaa cccagtggga aactagaatt
       61 ccacttttca gctcttctgt ctacggccaa tgggggcctc tgagctgaca atctcaggta
      121 gtttttgtag tttcctgggc tgtgctctag gatgtctgat gttgggggaa ctggcttagg
      181 gggagctcct tcaggcctct ctacattcct tcagggatca tgggctgtgt ctgcagctca
      241 aaccccgaag atgactggat ggagaacatt gacgtgtgtg aaaactgcca ctatcccata
      301 gtcccactgg acagcaagag tacgctgccc atccggaccg gctctgaagt tcgggaccca
      361 ctggtcacct atgagggctc cctcccacca gcctccccgc tgcaagacaa cctagttatt
      421 gccctgcaca gctatgagcc ctcccatgat ggagacttgg gctttgaaaa gggtgaacag
      481 ctccgaatcc tggagcagag cggtgagtgg tggaaggctc agtccctgac cactggccaa
      541 gaaggcttca tccccttcaa cttcgtggcg aaagcaaaca gcctggagcc tgaaccttgg
      601 ttcttcaaaa atctgagccg taaggacgcc gagcggcagc ttttggcgcc cgggaacacg
      661 catggatcct tcctgatccg ggaaagcgaa agcaccgccg gatccttttc actgtcagtc
      721 agagacttcg accagaacca gggagaagtg gtgaaacatt acaagatccg taacctagac
      781 aacggtggct tctacatctc ccctcgtatc acttttcccg gactacacga tctggtccgc
      841 cattacacca acgcctctga tgggctgtgc acaaaattga gccgtccttg ccagacccag
      901 aagccccaga aaccatggtg ggaggacgaa tgggaagttc ccagagagac attgaagttg
      961 gttgagcggc tgggagctgg ccagttcggg gaagtgtgga tgggatacta caatggacac
     1021 acgaaggtgg cagtgaaaag tctgaagcaa gggagtatgt ctcctgatgc cttcctggca
     1081 gaggctaacc tcatgaagca gctgcagcac ccgcggctcg tccggcttta tgcagtggtc
     1141 acccaggagc ccatctacat tatcacagaa tacatggaga acgggagcct agtagatttc
     1201 ctcaagactc cctcgggcat caagttgaac gtcaacaaac ttttggacat ggcagcccag
     1261 attgcagagg gcatggcgtt catcgaagag cagaattaca tccatcgtga cctgcgggct
     1321 gccaacatcc tggtgtctga cacgctgagc tgcaaaattg cagactttgg cctggcacgt
     1381 ctgattgagg acaatgaata cacggcccgg gagggggcca agtttcccat taagtggaca
     1441 gcaccagaag ccattaacta tgggaccttt accatcaagt cagatgtgtg gtccttcggg
     1501 atcctgctga cagagatcgt cacccatggc cggatccctt acccaggaat gaccaaccct
     1561 gaagtcattc agaacctgga aaaaggctac cgaatggtgc gacctgacaa ctgcccggaa
     1621 gagctgtacc acctcatgat gctgtgctgg aaggagcgcc cagaggacag gcccacgttt
     1681 gactaccttc ggagcgttct ggatgacttc ttcacagcca cagagggcca gtaccagccc
     1741 cagccttgag gccttgcggt cccaagatcc tcccctatcc ctctggagtt cttgtgccct
     1801 atccatgcgg cctgtgcaca cgtggactcc gactcggcac gtgaagcctg tgcgtgtgcc
     1861 atacatagcc gtttttgtct catacacaca tccgtagtta tgtgggttct acacatgtgc
     1921 cttataccca tggaacatgt gagtctaagt cttagagacc acctaaggtg tctctttcca
     1981 ttccattttc tgaaaccaca gagaggagaa ggcttgtgat tgctgcatgt gtctgtctac
     2041 cactgccttt ctgggggcct ccaggaaggc cctccttgtg ggctgccatc caatctaatg
     2101 tctctgtgtg ctgtgccttg gtggtaccac acaccaggag ctcaataaaa gtctgttcaa
     2161 ttaaggctgt
//
