LOCUS       NP_001094179             509 aa            linear   ROD 14-FEB-2025
DEFINITION  proto-oncogene tyrosine-protein kinase LCK [Rattus norvegicus].
ACCESSION   NP_001094179 XP_001061667 XP_001061773 XP_001062229 XP_232763
VERSION     NP_001094179.1
DBSOURCE    REFSEQ: accession NM_001100709.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 509)
  AUTHORS   Xuan,J., Zhang,J., He,J., Zhao,K., Miao,S., Chen,G. and Wei,S.
  TITLE     RNA-Sequencing Analysis and Expression of Lymphocyte-Specific
            Protein Tyrosine Kinase, Linker for Activation of T Cells, and
            70-kDa T-Cell Receptor Zeta-Chain Associated Protein Kinase in Rat
            Liver Transplant Rejection
  JOURNAL   Exp Clin Transplant 21 (12), 961-972 (2023)
   PUBMED   38263783
  REMARK    GeneRIF: RNA-Sequencing Analysis and Expression of
            Lymphocyte-Specific Protein Tyrosine Kinase, Linker for Activation
            of T Cells, and 70-kDa T-Cell Receptor Zeta-Chain Associated
            Protein Kinase in Rat Liver Transplant Rejection.
REFERENCE   2  (residues 1 to 509)
  AUTHORS   Ono,A., Hirooka,K., Nakano,Y., Nitta,E., Nishiyama,A. and
            Tsujikawa,A.
  TITLE     Gene expression changes in the retina after systemic administration
            of aldosterone
  JOURNAL   Jpn J Ophthalmol 62 (4), 499-507 (2018)
   PUBMED   29713904
REFERENCE   3  (residues 1 to 509)
  AUTHORS   Kochl,R., Thelen,F., Vanes,L., Brazao,T.F., Fountain,K., Xie,J.,
            Huang,C.L., Lyck,R., Stein,J.V. and Tybulewicz,V.L.
  TITLE     WNK1 kinase balances T cell adhesion versus migration in vivo
  JOURNAL   Nat Immunol 17 (9), 1075-1083 (2016)
   PUBMED   27400149
  REMARK    Erratum:[Nat Immunol. 2017 Jan 19;18(2):246. doi:
            10.1038/ni0217-246a.. PMID: 28102214]
REFERENCE   4  (residues 1 to 509)
  AUTHORS   Li,J.P., Yang,C.Y., Chuang,H.C., Lan,J.L., Chen,D.Y., Chen,Y.M.,
            Wang,X., Chen,A.J., Belmont,J.W. and Tan,T.H.
  TITLE     The phosphatase JKAP/DUSP22 inhibits T-cell receptor signalling and
            autoimmunity by inactivating Lck
  JOURNAL   Nat Commun 5, 3618 (2014)
   PUBMED   24714587
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 509)
  AUTHORS   Hui,E. and Vale,R.D.
  TITLE     In vitro membrane reconstitution of the T-cell receptor proximal
            signaling network
  JOURNAL   Nat Struct Mol Biol 21 (2), 133-142 (2014)
   PUBMED   24463463
REFERENCE   6  (residues 1 to 509)
  AUTHORS   Alai,M., Mui,A.L., Cutler,R.L., Bustelo,X.R., Barbacid,M. and
            Krystal,G.
  TITLE     Steel factor stimulates the tyrosine phosphorylation of the
            proto-oncogene product, p95vav, in human hemopoietic cells
  JOURNAL   J Biol Chem 267 (25), 18021-18025 (1992)
   PUBMED   1381360
REFERENCE   7  (residues 1 to 509)
  AUTHORS   Straus,D.B. and Weiss,A.
  TITLE     Genetic evidence for the involvement of the lck tyrosine kinase in
            signal transduction through the T cell antigen receptor
  JOURNAL   Cell 70 (4), 585-593 (1992)
   PUBMED   1505025
REFERENCE   8  (residues 1 to 509)
  AUTHORS   Molina,T.J., Kishihara,K., Siderovski,D.P., van Ewijk,W.,
            Narendran,A., Timms,E., Wakeham,A., Paige,C.J., Hartmann,K.U.,
            Veillette,A. et al.
  TITLE     Profound block in thymocyte development in mice lacking p56lck
  JOURNAL   Nature 357 (6374), 161-164 (1992)
   PUBMED   1579166
REFERENCE   9  (residues 1 to 509)
  AUTHORS   Barber,E.K., Dasgupta,J.D., Schlossman,S.F., Trevillyan,J.M. and
            Rudd,C.E.
  TITLE     The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase
            (p56lck) that phosphorylates the CD3 complex
  JOURNAL   Proc Natl Acad Sci U S A 86 (9), 3277-3281 (1989)
   PUBMED   2470098
REFERENCE   10 (residues 1 to 509)
  AUTHORS   Levinson,A.D., Oppermann,H., Levintow,L., Varmus,H.E. and
            Bishop,J.M.
  TITLE     Evidence that the transforming gene of avian sarcoma virus encodes
            a protein kinase associated with a phosphoprotein
  JOURNAL   Cell 15 (2), 561-572 (1978)
   PUBMED   214242
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On or before May 31, 2008 this sequence version replaced
            XP_001061773.1, XP_001061667.1, XP_232763.2, XP_001062229.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC160881.1, SRR26643284.7729.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     Protein         1..509
                     /product="proto-oncogene tyrosine-protein kinase LCK"
                     /EC_number="2.7.10.2"
                     /note="lymphocyte-specific protein tyrosine kinase;
                     lymphocyte cell-specific protein-tyrosine kinase;
                     lymphocyte protein tyrosine kinase"
                     /calculated_mol_wt=57759
     Region          2..72
                     /region_name="Interactions with CD4 and CD8.
                     /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (Q01621.3)"
     Region          65..118
                     /region_name="SH3_Lck"
                     /note="Src homology 3 domain of Lck Protein Tyrosine
                     Kinase; cd12005"
                     /db_xref="CDD:212938"
     Site            order(70,72,75..76,79,95..97,110,112,114..115)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212938"
     Site            102
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P06239; propagated from
                     UniProtKB/Swiss-Prot (Q01621.3)"
     Region          123..223
                     /region_name="SH2_Src_Lck"
                     /note="Src homology 2 (SH2) domain in lymphocyte cell
                     kinase (Lck); cd10362"
                     /db_xref="CDD:198225"
     Site            order(134,154,156..159,164,180,182,215)
                     /site_type="other"
                     /note="autoinhibitory site [polypeptide binding]"
                     /db_xref="CDD:198225"
     Site            order(134,154,156..158,164,180..182)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198225"
     Region          154..242
                     /region_name="Interaction with PTPRH.
                     /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (Q01621.3)"
     Site            159
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:P06239; propagated from
                     UniProtKB/Swiss-Prot (Q01621.3)"
     Site            162
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P06239; propagated from
                     UniProtKB/Swiss-Prot (Q01621.3)"
     Site            order(181,193..194,196,209,214..216)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198225"
     Site            192
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q01621.3)"
     Site            194
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P06239; propagated from
                     UniProtKB/Swiss-Prot (Q01621.3)"
     Region          237..500
                     /region_name="PTKc_Lck_Blk"
                     /note="Catalytic domain of the Protein Tyrosine Kinases,
                     Lymphocyte-specific kinase and Blk; cd05067"
                     /db_xref="CDD:270652"
     Site            order(251,253..255,259,271,273,288,316..319,323,364,
                     368..369,371,382,399..403,412,446)
                     /site_type="active"
                     /db_xref="CDD:270652"
     Site            order(251,254,259,271,273,288,316..319,323,364,366,369,
                     371,382)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270652"
     Site            order(265,267,299,302,304,317,343,347,375,495)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270652"
     Site            order(353,356..357,420,482..484,486,488..489,492,496,
                     499..500)
                     /site_type="other"
                     /note="CSK binding interface [polypeptide binding]"
                     /db_xref="CDD:270652"
     Site            order(364,368,399..403,412,446)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270652"
     Site            order(381..390,392..405)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270652"
     Site            394
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine, by autocatalysis.
                     /evidence=ECO:0000250|UniProtKB:P06239; propagated from
                     UniProtKB/Swiss-Prot (Q01621.3)"
     Site            505
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q01621.3)"
     CDS             1..509
                     /gene="Lck"
                     /gene_synonym="Lck1; Lcktkr; p56-LCK"
                     /coded_by="NM_001100709.2:220..1749"
                     /db_xref="GeneID:313050"
                     /db_xref="RGD:2994"
ORIGIN      
        1 mgcvcssnpe ddwmenidvc enchypivpl dskstlpirt gsevrdplvt yegslppasp
       61 lqdnlvialh syepshdgdl gfekgeqlri leqsgewwka qslttgqegf ipfnfvakan
      121 slepepwffk nlsrkdaerq llapgnthgs fliresesta gsfslsvrdf dqnqgevvkh
      181 ykirnldngg fyispritfp glhdlvrhyt nasdglctkl srpcqtqkpq kpwwedewev
      241 pretlklver lgagqfgevw mgyynghtkv avkslkqgsm spdaflaean lmkqlqhprl
      301 vrlyavvtqe piyiiteyme ngslvdflkt psgiklnvnk lldmaaqiae gmafieeqny
      361 ihrdlraani lvsdtlscki adfglarlie dneytarega kfpikwtape ainygtftik
      421 sdvwsfgill teivthgrip ypgmtnpevi qnlekgyrmv rpdncpeely hlmmlcwker
      481 pedrptfdyl rsvlddffta tegqyqpqp
//
