LOCUS       NM_032613               3382 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus LIM and SH3 protein 1 (Lasp1), mRNA.
ACCESSION   NM_032613
VERSION     NM_032613.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,X., Zhang,H., Yin,H., Geng,S., Liu,Y., Liu,C., Zhao,J., Liu,Y.,
            Wang,X. and Wang,Y.
  TITLE     Up-regulated microRNA-218-5p ameliorates the damage of dopaminergic
            neurons in rats with Parkinson's disease via suppression of LASP1
  JOURNAL   Brain Res Bull 166, 92-101 (2021)
   PUBMED   33144090
  REMARK    GeneRIF: Up-regulated microRNA-218-5p ameliorates the damage of
            dopaminergic neurons in rats with Parkinson's disease via
            suppression of LASP1.
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Pollitt,S.L., Myers,K.R., Yoo,J. and Zheng,J.Q.
  TITLE     LIM and SH3 protein 1 localizes to the leading edge of protruding
            lamellipodia and regulates axon development
  JOURNAL   Mol Biol Cell 31 (24), 2718-2732 (2020)
   PUBMED   32997597
  REMARK    GeneRIF: LIM and SH3 protein 1 localizes to the leading edge of
            protruding lamellipodia and regulates axon development.
REFERENCE   3  (bases 1 to 3382)
  AUTHORS   Nicholson,L., Lindsay,L. and Murphy,C.R.
  TITLE     Change in distribution of cytoskeleton-associated proteins, lasp-1
            and palladin, during uterine receptivity in the rat endometrium
  JOURNAL   Reprod Fertil Dev 30 (11), 1482-1490 (2018)
   PUBMED   29739492
  REMARK    GeneRIF: Lasp-1 was increased in the uterus at the time of
            implantation compared with the time of fertilization.
REFERENCE   4  (bases 1 to 3382)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3382)
  AUTHORS   Stolting,M., Wiesner,C., van Vliet,V., Butt,E., Pavenstadt,H.,
            Linder,S. and Kremerskothen,J.
  TITLE     Lasp-1 regulates podosome function
  JOURNAL   PLoS One 7 (4), e35340 (2012)
   PUBMED   22514729
  REMARK    GeneRIF: Data indicate that Lasp-1 is a novel component of
            podosomes and is involved in the regulation of podosomal function.
REFERENCE   6  (bases 1 to 3382)
  AUTHORS   Kuo,J.C., Han,X., Hsiao,C.T., Yates,J.R. 3rd and Waterman,C.M.
  TITLE     Analysis of the myosin-II-responsive focal adhesion proteome
            reveals a role for beta-Pix in negative regulation of focal
            adhesion maturation
  JOURNAL   Nat Cell Biol 13 (4), 383-393 (2011)
   PUBMED   21423176
REFERENCE   7  (bases 1 to 3382)
  AUTHORS   Gray,C.H., McGarry,L.C., Spence,H.J., Riboldi-Tunnicliffe,A. and
            Ozanne,B.W.
  TITLE     Novel beta-propeller of the BTB-Kelch protein Krp1 provides a
            binding site for Lasp-1 that is necessary for pseudopodial
            extension
  JOURNAL   J Biol Chem 284 (44), 30498-30507 (2009)
   PUBMED   19726686
  REMARK    GeneRIF: binding sites necessary for Krp1-Lasp1 interaction in
            vitro and function in vivo
REFERENCE   8  (bases 1 to 3382)
  AUTHORS   Keicher,C., Gambaryan,S., Schulze,E., Marcus,K., Meyer,H.E. and
            Butt,E.
  TITLE     Phosphorylation of mouse LASP-1 on threonine 156 by cAMP- and
            cGMP-dependent protein kinase
  JOURNAL   Biochem Biophys Res Commun 324 (1), 308-316 (2004)
   PUBMED   15465019
REFERENCE   9  (bases 1 to 3382)
  AUTHORS   Phillips,G.R., Anderson,T.R., Florens,L., Gudas,C., Magda,G.,
            Yates,J.R. 3rd and Colman,D.R.
  TITLE     Actin-binding proteins in a postsynaptic preparation: Lasp-1 is a
            component of central nervous system synapses and dendritic spines
  JOURNAL   J Neurosci Res 78 (1), 38-48 (2004)
   PUBMED   15372503
  REMARK    GeneRIF: Lasp-1 is strongly expressed by central nervous system
            neurons and is concentrated at synaptic sites.
REFERENCE   10 (bases 1 to 3382)
  AUTHORS   Chew,C.S., Parente,J.A. Jr., Chen,X., Chaponnier,C. and
            Cameron,R.S.
  TITLE     The LIM and SH3 domain-containing protein, lasp-1, may link the
            cAMP signaling pathway with dynamic membrane restructuring
            activities in ion transporting epithelia
  JOURNAL   J Cell Sci 113 (Pt 11), 2035-2045 (2000)
   PUBMED   10806114
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On Nov 27, 2020 this sequence version replaced NM_032613.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC099791.1, SRR26643299.45321.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-155               JAXUCZ010000010.1  83291518-83291672
            156-250             JAXUCZ010000010.1  83298958-83299052
            251-335             JAXUCZ010000010.1  83307922-83308006
            336-443             JAXUCZ010000010.1  83317143-83317250
            444-600             JAXUCZ010000010.1  83326257-83326413
            601-704             JAXUCZ010000010.1  83326741-83326844
            705-3165            JAXUCZ010000010.1  83329292-83331752
            3166-3382           JAXUCZ010000010.1  83331771-83331987
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q31"
     gene            1..3382
                     /gene="Lasp1"
                     /note="LIM and SH3 protein 1"
                     /db_xref="GeneID:29278"
                     /db_xref="RGD:68408"
     exon            1..155
                     /gene="Lasp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..878
                     /gene="Lasp1"
                     /note="LASP-1"
                     /codon_start=1
                     /product="LIM and SH3 domain protein 1"
                     /protein_id="NP_116002.1"
                     /db_xref="GeneID:29278"
                     /db_xref="RGD:68408"
                     /translation="MNPNCARCGKIVYPTEKVNCLDKFWHKACFHCETCKMTLNMKNY
                     KGYEKKPYCNAHYPKQSFTMVADTPENLRLKQQSELQSQVRYKEEFEKNKGKGFSVVA
                     DTPELQRIKKTQDQISNIKYHEEFEKSRMGPSGGEGIEPERREAQDSSSYRRPTEQQQ
                     PQPHHIPTSAPVYQQPQQQQVTPSYGGYKEPAAPVSIQRSAPGGGGKRYRAVYDYSAA
                     DEDEVSFQDGDTIVNVQQIDDGWMYGTVERTGDTGMLPANYVEAI"
     exon            156..250
                     /gene="Lasp1"
                     /inference="alignment:Splign:2.1.0"
     exon            251..335
                     /gene="Lasp1"
                     /inference="alignment:Splign:2.1.0"
     exon            336..443
                     /gene="Lasp1"
                     /inference="alignment:Splign:2.1.0"
     exon            444..600
                     /gene="Lasp1"
                     /inference="alignment:Splign:2.1.0"
     exon            601..704
                     /gene="Lasp1"
                     /inference="alignment:Splign:2.1.0"
     exon            705..3382
                     /gene="Lasp1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gccgccgcct cctcctacca gctcgcccaa ggaaaaggga acgcgcgcga gcacgggcgt
       61 tcccgcccca gtctttcccc ggaaccatga accctaactg tgcccggtgc ggcaaaatcg
      121 tgtaccccac ggagaaggtg aactgtctgg ataagttctg gcataaagca tgctttcact
      181 gcgagacctg caagatgact ttgaacatga agaactacaa gggttacgag aagaagcctt
      241 actgcaatgc acactacccc aagcagtcct tcaccatggt ggccgacact ccggagaacc
      301 tccgcctgaa gcaacagagc gagctgcaga gtcaggtgcg ttacaaggag gagtttgaga
      361 agaacaaggg caaaggcttc agtgtggtgg cagacacgcc tgaactgcag agaatcaaga
      421 agacccagga ccagatcagc aatatcaaat accatgagga gtttgagaag agccgcatgg
      481 ggcccagcgg aggtgaaggc atagagccag agcgccgaga agcccaggac agcagcagct
      541 accggaggcc cacggagcag caacagccac agccccacca tatcccgacc agtgccccag
      601 tttaccagca gccccaacag cagcaggtga ccccgtccta tggtgggtac aaggagccag
      661 cagcccctgt ctccatacag cgcagtgccc caggtggcgg cgggaaacgg taccgtgcag
      721 tgtatgacta cagcgctgcc gatgaggacg aggtctcctt ccaggatggg gacaccatcg
      781 tcaatgtgca gcagatcgat gatggctgga tgtatgggac cgtagagcgc accggtgaca
      841 cggggatgct gccagccaac tatgttgagg ccatctgagc cccgtgctgc cccaccctgt
      901 cttcaatgca ttccatggca tcacatctgt cctggggcct gacccatcca cccttcagtg
      961 tctctgtctc ttaagatctt caactgcttc tttatccccg cccctcctcc agcttccttt
     1021 gccaacctaa gccttgttct gctcctgtcg tgggctcctt cctctggcag gtttcccttg
     1081 gaccaatcaa ctgattgatt tttctctctg gatggaacag gctgggcact ctgaggaggg
     1141 caggattgtt ctgaggaggg tgggatcaca gggctgggct gaaacgagag acccattaag
     1201 gagatcggtt agactgggtg tggtggcata cacctttaat cccagcagag gattcatccc
     1261 agcaaagggg ggaagaggca ggcagatctg tgactttgaa gccagtctgg tctacatcat
     1321 gagtttcatg acggccagag ctatgtagtg agaccctgtc tcgagggagg agggactggt
     1381 tagctcttag cttcacttcc caccttaggc ccgttggaac ccctggccca gctcctctgc
     1441 acccttctgc ctagagctgg ggtgagctgc atactcctca tcccaccagg tggggctcca
     1501 cacattccca gggggtgggt gttaaggcct cagttatgta aagagcaatg tgatggcctt
     1561 cccctagtcc atctctctgc ctcctcaggg catccagggg agggcagact ttctgcttca
     1621 ggctgaatcc ctgcccctct gttccttcac acactggagt gtgccattct gcccacacaa
     1681 acctagggcg gccaaagagc tccccagaaa tcccagcttg ggtcagattc ttgccttagt
     1741 tctaggggca gcagcagaaa ggagggaacg gggtgttctc ctgggcccga ttgcttccct
     1801 cctcccccag cctcctggga ctcattatct ccctggctgg gagcctgctg tgaagggtag
     1861 agcaagggaa gcctttggga cttgactgag ctgtttcttc ctgctccgct ggggaaaggt
     1921 ctctgtgtct cctatggcct tccttccctg gaacaaggct ggactagcct agagctctgc
     1981 agtgacagaa aaatggcaag agttttctgg agcacaggag gccctcccac ctccgcagtg
     2041 aggtgggaga aggcttctgc gttagatgtg tgctttcctg tctttgtgtc tgaggctaca
     2101 gagggaccaa gtcacccaca cactgtggca cctcacctga ggctcttctg tccttggagc
     2161 aaaggaggga gtccagggct ccaaggctgg cggcaccatc tctgggctgg tgctaaactc
     2221 aagttcctcc tttgcatctg gggcccggga cagtactcac tcgatttcat tcatctcggg
     2281 cttttcttgt cactcctcct gggtctcctg actcatcttt accctgagtg tgatgtcagg
     2341 tccctttaca tactcagacc ctgctgcagg tgacctttga acctgaagac cttggccagg
     2401 ctagcagtgt ggtgtcatga gggcttgcta gagtaaacca tcttcctgcc tggtcaatcc
     2461 ttttacctcc gaccctagcc tgggtgaagg caaggagtgt tctggtgggc aacatgggga
     2521 gaaccttgta ggagcatctc aggagctact ggggaggtgc cccacccact ccacttcccc
     2581 ttgatctcaa agcacaatgc ggttctgggg accagggttc aggggcgctt gctcttggag
     2641 ggcctgtgtt caggaggtgc tacaagcctc ctggttcctc tcagctgagc ctcccttccc
     2701 aacatgtcaa gtcagctgct cctttagtgc cctagaactg ggggagactt tcccacacct
     2761 gcagagggaa gaggaactac caaaggtgct gagggcccca agcctgctgg agtccatact
     2821 tttccagaag catccaaccc tagaagagca cagaatcccc aagcccatgg tctcttcctt
     2881 tgtaaaggtc ttttcagcca atcaaaaccc aggaaagagg gcgccttggc tagactgtga
     2941 acttaacttt tgtggaccaa tcctgtagcg caagaagccc cgtggtgagg agcatgagtt
     3001 cttcccatgc tgcttcctgt cccctcctca tgtccctcca gggaacattt catttcctaa
     3061 tagctggaca caagacaaaa agaaccaccc cacagagcct gtatttaaac agaaacagaa
     3121 gtgaccctgt gaagtccacc tctgtctaac atttcatccg tgtttgtgtg tgtgtgtgtg
     3181 tgtgtgtgtg tgtgcgtgtg aagccaccac tcatctttta tatcggattg tcatctttct
     3241 ttggtctgtt tggtcccctc cctcctggcc ttgtgcttgg gatcaattct ttctggcctg
     3301 ttatgatttt tgaacattga cttgaaccac aaagtgaatc tttatcctgg tgactcaaat
     3361 aaaagtataa tttttacctg cg
//
