LOCUS       NM_001413842            3894 bp    mRNA    linear   ROD 22-SEP-2024
DEFINITION  Rattus norvegicus leucyl-tRNA synthetase 2, mitochondrial (Lars2),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001413842 XM_006244201
VERSION     NM_001413842.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Riley,L.G., Rudinger-Thirion,J., Schmitz-Abe,K., Thorburn,D.R.,
            Davis,R.L., Teo,J., Arbuckle,S., Cooper,S.T., Campagna,D.R.,
            Frugier,M., Markianos,K., Sue,C.M., Fleming,M.D. and
            Christodoulou,J.
  TITLE     LARS2 Variants Associated with Hydrops, Lactic Acidosis,
            Sideroblastic Anemia, and Multisystem Failure
  JOURNAL   JIMD Rep 28, 49-57 (2016)
   PUBMED   26537577
REFERENCE   3  (bases 1 to 3894)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   4  (bases 1 to 3894)
  AUTHORS   Bullard,J.M., Cai,Y.C. and Spremulli,L.L.
  TITLE     Expression and characterization of the human mitochondrial
            leucyl-tRNA synthetase
  JOURNAL   Biochim Biophys Acta 1490 (3), 245-258 (2000)
   PUBMED   10684970
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Nov 9, 2022 this sequence version replaced XM_006244201.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360186.2598892.1,
                                           SRR26643429.32756.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-168               JAXUCZ010000008.1  131887756-131887923
            169-417             JAXUCZ010000008.1  131893721-131893969
            418-546             JAXUCZ010000008.1  131900269-131900397
            547-638             JAXUCZ010000008.1  131918175-131918266
            639-699             JAXUCZ010000008.1  131920185-131920245
            700-789             JAXUCZ010000008.1  131932875-131932964
            790-933             JAXUCZ010000008.1  131935300-131935443
            934-1041            JAXUCZ010000008.1  131941401-131941508
            1042-1201           JAXUCZ010000008.1  131941868-131942027
            1202-1306           JAXUCZ010000008.1  131951081-131951185
            1307-1422           JAXUCZ010000008.1  131955405-131955520
            1423-1706           JAXUCZ010000008.1  131959567-131959850
            1707-1805           JAXUCZ010000008.1  131961515-131961613
            1806-1943           JAXUCZ010000008.1  131965162-131965299
            1944-2044           JAXUCZ010000008.1  131973045-131973145
            2045-2227           JAXUCZ010000008.1  131973554-131973736
            2228-2397           JAXUCZ010000008.1  131974814-131974983
            2398-2475           JAXUCZ010000008.1  131976255-131976332
            2476-2587           JAXUCZ010000008.1  131977224-131977335
            2588-2715           JAXUCZ010000008.1  131980721-131980848
            2716-3894           JAXUCZ010000008.1  131982688-131983866
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q32"
     gene            1..3894
                     /gene="Lars2"
                     /note="leucyl-tRNA synthetase 2, mitochondrial"
                     /db_xref="GeneID:363172"
                     /db_xref="RGD:1308429"
     exon            1..168
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            169..417
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     CDS             187..2895
                     /gene="Lars2"
                     /EC_number="6.1.1.4"
                     /note="probable leucyl-tRNA synthetase, mitochondrial;
                     probable leucine--tRNA ligase, mitochondrial"
                     /codon_start=1
                     /product="leucine--tRNA ligase, mitochondrial"
                     /protein_id="NP_001400771.1"
                     /db_xref="GeneID:363172"
                     /db_xref="RGD:1308429"
                     /translation="MASACQRLSFCVSPLKRQLVSGLPVILWERIIPGCSRSIYSATG
                     KWTKEYTLQTRKDVEKWWHQQIKVQASKISEEDKLKPKFYLLSMFPYPSGKLHMGHVR
                     VYTLSDTIARFQKMRGMQVINPMGWDAFGLPAENAAIERNLHPESWTQSNIKHMRKQL
                     DRLGLCFSWDREITTCLPDYYKWTQYLFIKLYEAGLAYQKEALVNWDPVDQTVLANEQ
                     VNEYGCSWRSGAKVEKKYLRQWFIKTTAYAKAMQDALADLPEWYGIKEMQAHWIGDCV
                     GCHLDFTLKVDGQDTGEKLTAYTATPEAIYGISHVAISPSHGLLHGCSSLKTALQKAL
                     VPGRDCLTPVMAVNMLTLQEVPVVIMAKPDLEGSLDSKIGIPSTSSEDTRLAQALGLP
                     YSEVIETSPDGTERLSGSAEFTGMTRQDAFVALTRKARGMRVGGHVTSNKLKDWLVSR
                     QRYWGTPIPIVHCPACGPVPVPLQDLPVTLPSITSLTGRGGSPLASALEWVNCSCPRC
                     KGSAKRETDTMDTFVDSAWYYFRYTDPHNTHSPFSSALADFWMPVDLYIGGKEHAVMH
                     LFYARFFSHFCHRQKMVKHREPFHKLLAQGLIKGQTFHLLSGQCLKREDIDFTGPTPV
                     CAKTKERLEVTWEKMSKSKHNGVDPEEIVAQYGIDTIRLYILFAAPPEKDILWDVKTD
                     ALPGVLRWQQRLWSLTTRFIEARTSGTAPQPQLLNNKEKAKARSLWEYKNAVITQVTT
                     HFTEDFALNSVVSQLMGLSGALSQASQRLVLHSPEFEDALCALLVMAAPLAPHITSEL
                     WAGLALVPSKLCDRYAWDAGVMLQAWPTVDSQFLQKPDMVQMAVLINNKACGKIPVPQ
                     HVAQDQDKVHEFVLQSELGIKLLQGRSIRKAFLSPRTALINFLVKE"
     exon            418..546
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            547..638
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            639..699
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            700..789
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            790..933
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1041
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1201
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1306
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..1422
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            1423..1706
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1805
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            1806..1943
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            1944..2044
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            2045..2227
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            2228..2397
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            2398..2475
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            2476..2587
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            2588..2715
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
     exon            2716..3894
                     /gene="Lars2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gttgttttgc caacatggcg gcgcccatag tgactggcca ggtcttgttc gtgtaatgct
       61 tgaagaacga ggcagaaggg agcagctggc cagagcctgt gcacagaaag cccctactgg
      121 ggctcaggag gacgagagac aggccccact tagccccagg agttaaaggg tcttcccccc
      181 tgaagaatgg catcagcctg tcagaggctg agtttttgtg tctctcctct gaaaaggcag
      241 ctagttagtg ggctacctgt catcctatgg gaaagaataa ttccagggtg ttccagaagc
      301 atctacagcg ccaccgggaa gtggacaaag gagtacacac tgcagacaag gaaggacgtg
      361 gagaagtggt ggcaccaaca gataaaggtg caggcatcca aaatctcaga ggaagataaa
      421 ttaaagccca aattctactt gctgtccatg ttcccatatc cttctggcaa gctgcacatg
      481 ggccatgtcc gtgtctacac cctcagcgac accattgcac ggttccagaa gatgagaggc
      541 atgcaggtta tcaaccccat ggggtgggat gcgtttggat tgcctgctga aaatgctgcc
      601 atagagagga atttgcaccc agaaagctgg acccaaagca acattaaaca catgaggaaa
      661 cagctggacc gcctcggcct gtgcttcagc tgggacaggg aaatcactac gtgtttgcca
      721 gattactaca aatggactca atacctgttt atcaaactct acgaagctgg acttgcctat
      781 caaaaggagg ccttggttaa ctgggaccca gtggaccaaa cagtgctggc taatgagcag
      841 gtaaatgaat atggctgttc atggcgttct ggagccaagg tagagaagaa atacctcaga
      901 cagtggttca ttaagacaac tgcatacgca aaggccatgc aggatgcatt ggcagacctc
      961 ccagagtggt atggaataaa agaaatgcag gcccattgga ttggagactg cgtaggctgc
     1021 cacctggact tcacattaaa ggttgatgga caagacacag gagaaaaact gaccgcatat
     1081 acagccacac ctgaggccat ctatggcatc tcccatgtgg ccatctctcc tagccatggg
     1141 cttctacatg gctgcagctc tctgaagaca gccttgcaga aggcactggt ccctggcaga
     1201 gattgcctca caccagtgat ggccgtgaac atgctcaccc tgcaggaggt ccccgtcgtt
     1261 atcatggcta agcctgactt ggaaggctct ctagattcaa aaataggaat ccccagcacc
     1321 agctcggagg acaccagact agcccaagct ctgggcctac cctactctga ggtcattgaa
     1381 acttccccag atggcacaga gagactgagt ggatctgctg agtttacagg tatgacccgg
     1441 caggatgcct ttgttgccct gactcggaaa gcccgtggga tgagagtggg tggacacgtg
     1501 acaagtaaca agctgaagga ctggctggtc tcaaggcagc gctactgggg cacacccatc
     1561 cccattgtcc actgcccagc ctgtggccct gtgcctgtgc cactgcaaga cttgcctgtg
     1621 actttgccca gcatcacatc tctaacgggc agagggggct ccccgctggc ctcggctttg
     1681 gagtgggtaa actgctcctg cccaaggtgc aagggatcag ccaagagaga gacagacacc
     1741 atggacacct ttgtggattc tgcatggtac tacttcagat acacggaccc tcacaatact
     1801 catagtcctt tcagctcggc actggcagac ttctggatgc ctgtggatct gtacatcgga
     1861 gggaaagagc atgctgtcat gcacttgttc tatgcacgat tctttagtca tttttgccac
     1921 cgtcagaaga tggtgaagca cagggagcct ttccataaac tcctggccca aggcctcatt
     1981 aagggacaga catttcacct tctgtctggg cagtgtctga agagggaaga catagatttc
     2041 acaggtccta ctcctgtttg tgcaaaaacg aaagagaggt tagaggtgac gtgggagaag
     2101 atgagcaagt ccaaacacaa tggcgtggac cctgaggaga ttgtggccca gtatggaatt
     2161 gacacaatcc ggctctacat cctctttgca gcccctcctg agaaggacat cttgtgggat
     2221 gtgaagaccg atgcacttcc tggagtgcta agatggcaac agcggttgtg gtccctgaca
     2281 acaagattca ttgaggcaag gacttccggg acagcccccc agcctcagct gctgaacaac
     2341 aaggagaaag ccaaggcccg aagcctctgg gagtacaaaa acgcagtcat aactcaggtg
     2401 accacccatt tcacagagga ctttgccctg aattccgtag tttctcagct gatgggactc
     2461 agcggtgccc tctcgcaagc ctctcagaga ctggttctcc acagccctga gtttgaggat
     2521 gctctgtgtg ctctgctggt gatggctgcc cccctggccc ctcatataac ctcagaactc
     2581 tgggcaggcc tggcgctggt accaagcaag ctctgtgacc ggtatgcctg ggatgctggt
     2641 gtgatgcttc aggcctggcc cactgtggac tcacagtttc ttcagaagcc tgatatggtt
     2701 cagatggctg ttctgatcaa caacaaagcc tgtggcaaga tccctgtgcc ccagcatgtt
     2761 gcccaagacc aggacaaagt ccatgaattt gttctccaga gtgaactagg catcaagctg
     2821 ctgcagggga gaagcatcag gaaggccttc ctctccccca ggacggccct catcaacttc
     2881 ttggtgaagg agtgactggg gcttcctcct ctgtccagat caagggaagg aatgttagct
     2941 gagacaatga gacaaacatc agggacattg gcttttgtgc caggaccaac tggagtcagc
     3001 aggaggcctg tgtgctgcag tctagaccta ggtgagaatg gatgtgggca aagacgaatg
     3061 agaggagaag attcattcag ttgtcctgta cccctccact tccactcagg gcatgaagcc
     3121 gccagagatg ctgccgagtt ggagtaagaa gcgggcaagg ctggcgagct cgccaggagc
     3181 tttgcacctc accatggtgc tgtctcaggt tcttcccagt ggtcgggtag accagaggag
     3241 ccacttaggc ccaggacctg cacaatagtt ggctgttcaa ctgtggaact ggacatggac
     3301 acaggtgtct ctgcaaattg ttctgcagat gtctccagca cccttcaact atgtcacaca
     3361 ggtctcgtcc actcccaccg ggtggtctgt tctgagttta atgaatgctc tgggacagga
     3421 ctccactttt gagtctgaac agaaggactg aatcacagcc actgtttcaa catgtagagt
     3481 gtgctctgtg gtgagtagag cattgttctg agctgctgat cctcaccaga accccatcac
     3541 agcccagtgg ggggcttgaa ccccagatgg ctcacagggg ccccagatag tgacaccact
     3601 gtcattgagt tggcccctgt agtgttacta acaaaagcaa gttcatcaga gaagaacaga
     3661 actgttgctt cctgggtgtg acacgtcctg tgtgggagac agctatatca taacaggtgt
     3721 ggaccagtaa attaggcaaa gctgacttta tgtgggtcat cagtaaaatg ctattacagt
     3781 catgacctga acaagctctg tatctgtaat ctgaagtcac ccagaagtgg ctgtaatgtc
     3841 atctcatgcc ttcagatgcc ttttaatgtc ataaacatat cactgtaagt gcaa
//
