LOCUS       NM_199370               1695 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus keratin 8 (Krt8), mRNA.
ACCESSION   NM_199370 XM_343335
VERSION     NM_199370.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Wang,D., Shang,Q., Mao,J., Gao,C., Wang,J., Wang,D., Wang,H.,
            Jia,H., Peng,P., Du,M., Luo,Z. and Yang,L.
  TITLE     Phosphorylation of KRT8 (keratin 8) by excessive mechanical
            load-activated PKN (protein kinase N) impairs autophagosome
            initiation and contributes to disc degeneration
  JOURNAL   Autophagy 19 (9), 2485-2503 (2023)
   PUBMED   36897022
  REMARK    GeneRIF: Phosphorylation of KRT8 (keratin 8) by excessive
            mechanical load-activated PKN (protein kinase N) impairs
            autophagosome initiation and contributes to disc degeneration.
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Wogenstein,K.L., Szabo,S., Lunova,M., Wiche,G., Haybaeck,J.,
            Strnad,P., Boor,P., Wagner,M. and Fuchs,P.
  TITLE     Epiplakin deficiency aggravates murine caerulein-induced acute
            pancreatitis and favors the formation of acinar keratin granules
  JOURNAL   PLoS One 9 (9), e108323 (2014)
   PUBMED   25232867
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1695)
  AUTHORS   Bell,S.M., Zhang,L., Xu,Y., Besnard,V., Wert,S.E., Shroyer,N. and
            Whitsett,J.A.
  TITLE     Kruppel-like factor 5 controls villus formation and initiation of
            cytodifferentiation in the embryonic intestinal epithelium
  JOURNAL   Dev Biol 375 (2), 128-139 (2013)
   PUBMED   23266329
REFERENCE   4  (bases 1 to 1695)
  AUTHORS   Bordeleau,F., Myrand Lapierre,M.E., Sheng,Y. and Marceau,N.
  TITLE     Keratin 8/18 regulation of cell stiffness-extracellular matrix
            interplay through modulation of Rho-mediated actin cytoskeleton
            dynamics
  JOURNAL   PLoS One 7 (6), e38780 (2012)
   PUBMED   22685604
  REMARK    GeneRIF: Results indicate a keratins K8/K18 intermediate filament
            (IF) contribution to the cell stiffness-extracellular matrix (ECM)
            interplay through a modulation of Rho-dependent actin organization.
REFERENCE   5  (bases 1 to 1695)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   6  (bases 1 to 1695)
  AUTHORS   Caulin,C., Ware,C.F., Magin,T.M. and Oshima,R.G.
  TITLE     Keratin-dependent, epithelial resistance to tumor necrosis
            factor-induced apoptosis
  JOURNAL   J Cell Biol 149 (1), 17-22 (2000)
   PUBMED   10747083
REFERENCE   7  (bases 1 to 1695)
  AUTHORS   Ando,S., Tokui,T., Yano,T. and Inagaki,M.
  TITLE     Keratin 8 phosphorylation in vitro by cAMP-dependent protein kinase
            occurs within the amino- and carboxyl-terminal end domains
  JOURNAL   Biochem Biophys Res Commun 221 (1), 67-71 (1996)
   PUBMED   8660345
REFERENCE   8  (bases 1 to 1695)
  AUTHORS   Wang,X., Hsieh,J.T. and Zhau,H.E.
  TITLE     [Cloning and characterization of a specific cytokeratin-8 cDNA from
            rat prostatic epithelium]
  JOURNAL   Zhongguo Yi Xue Ke Xue Yuan Xue Bao 16 (1), 1-7 (1994)
   PUBMED   7525090
REFERENCE   9  (bases 1 to 1695)
  AUTHORS   Hsieh,J.T., Zhau,H.E., Wang,X.H., Liew,C.C. and Chung,L.W.
  TITLE     Regulation of basal and luminal cell-specific cytokeratin
            expression in rat accessory sex organs. Evidence for a new class of
            androgen-repressed genes and insight into their pairwise control
  JOURNAL   J Biol Chem 267 (4), 2303-2310 (1992)
   PUBMED   1370816
REFERENCE   10 (bases 1 to 1695)
  AUTHORS   Yano,T., Tokui,T., Nishi,Y., Nishizawa,K., Shibata,M., Kikuchi,K.,
            Tsuiki,S., Yamauchi,T. and Inagaki,M.
  TITLE     Phosphorylation of keratin intermediate filaments by protein kinase
            C, by calmodulin-dependent protein kinase and by cAMP-dependent
            protein kinase
  JOURNAL   Eur J Biochem 197 (2), 281-290 (1991)
   PUBMED   1709097
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AY464139.1.
            
            On Jan 11, 2004 this sequence version replaced XM_343335.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY464139.1, BC097497.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132303, SAMD00132305
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q36"
     gene            1..1695
                     /gene="Krt8"
                     /gene_synonym="CYKER; Krt2-8"
                     /note="keratin 8"
                     /db_xref="GeneID:25626"
                     /db_xref="RGD:2984"
     CDS             1..1452
                     /gene="Krt8"
                     /gene_synonym="CYKER; Krt2-8"
                     /note="K8; CK-8; cytokeratin-8; cytokeratin endo A;
                     type-II keratin Kb8; keratin complex 2, basic, gene 8;
                     keratin 8, type II"
                     /codon_start=1
                     /product="keratin, type II cytoskeletal 8"
                     /protein_id="NP_955402.1"
                     /db_xref="GeneID:25626"
                     /db_xref="RGD:2984"
                     /translation="MSVRVTQKSYKMSTSGPRAFSSRSFTSGPGARISSSSFSRVGSS
                     SSSFRGSLGGFGGAGVGGITAVTVNQSLLNPLKLEVDPNIQAVRTQEKEQIKTLNNKF
                     ASFIDKVRFLEQQNKMLETKWSLLQQQKTSRSNMDNMFESYINNLRRQLEALGQEKLK
                     LEVELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLT
                     DEINFLRQIHEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVRAQYEEIANRSRA
                     EAETMYQIKYEELQTLAGKHGDDLRRSKTEISEMNRNISRLQAEIDALKGQRATLEAA
                     IADAEQRGELAVKDANAKLEDLKNALQKAKQDMARQLREYQELMNVKLALDIEIATYR
                     KLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSSYGGLTSPGFSYGMSSFQPGFGSVG
                     GSSTYSRTKAVVVKKIETRDGKLVSESSDIMSK"
     exon            1..324
                     /gene="Krt8"
                     /gene_synonym="CYKER; Krt2-8"
                     /inference="alignment:Splign:2.1.0"
     exon            325..533
                     /gene="Krt8"
                     /gene_synonym="CYKER; Krt2-8"
                     /inference="alignment:Splign:2.1.0"
     exon            534..594
                     /gene="Krt8"
                     /gene_synonym="CYKER; Krt2-8"
                     /inference="alignment:Splign:2.1.0"
     exon            595..690
                     /gene="Krt8"
                     /gene_synonym="CYKER; Krt2-8"
                     /inference="alignment:Splign:2.1.0"
     exon            691..855
                     /gene="Krt8"
                     /gene_synonym="CYKER; Krt2-8"
                     /inference="alignment:Splign:2.1.0"
     exon            856..981
                     /gene="Krt8"
                     /gene_synonym="CYKER; Krt2-8"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1202
                     /gene="Krt8"
                     /gene_synonym="CYKER; Krt2-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..1261
                     /gene="Krt8"
                     /gene_synonym="CYKER; Krt2-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1666
                     /gene="Krt8"
                     /gene_synonym="CYKER; Krt2-8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atgtccgtca gggtgactca gaaatcctac aagatgtcca cctccggtcc ccgggccttc
       61 agcagccgct cgttcacgag tggacccggt gcccgcatca gctcttccag cttttcccgg
      121 gtgggcagca gcagcagcag cttccgcgga agcctgggcg gctttggcgg ggctggtgtc
      181 gggggcatca cggcggtcac ggtgaaccag agcctgctga accccttgaa gctggaggtg
      241 gaccccaaca tccaggctgt gcgcactcag gagaaagagc agatcaagac cctgaacaac
      301 aagttcgcct ctttcattga caaggtacgc ttcctggagc agcagaacaa gatgctggag
      361 accaaatgga gcttgctgca acagcagaag acatccagga gcaacatgga caacatgttt
      421 gagagttaca tcaacaacct ccgtcggcag ctggaagccc tgggccagga gaagctgaag
      481 ctggaggtgg agcttggcaa catgcagggc ctggtggagg acttcaagaa taagtatgag
      541 gatgagatca acaagcgtac agagatggag aatgagtttg tcctcatcaa gaaggatgtg
      601 gatgaagcct acatgaacaa ggtggagctt gagtcccgcc tggaaggact gaccgacgag
      661 atcaacttcc tccggcagat ccatgaagag gagatccgtg agctgcagtc tcagatctca
      721 gacacgtctg tggtgctgtc catggacaac agccgctccc tggacatgga cagcatcatt
      781 gctgaagttc gtgcccagta tgaggagatc gccaaccgca gccgagctga ggccgaaacc
      841 atgtaccaga ttaagtatga ggaattgcag accctggctg ggaagcacgg ggatgatcta
      901 cgtcgctcga agacggagat ctctgagatg aaccgtaaca tcagccgcct gcaggcggag
      961 attgacgccc tcaaaggcca gagggcaacc ctggaggcgg ccattgctga tgcagagcag
     1021 cgtggggaac tggccgtgaa ggatgccaat gccaagctgg aggatctgaa gaatgccctg
     1081 cagaaggcca agcaggacat ggcccggcag ctgcgcgagt accaggagct gatgaacgtc
     1141 aagctggcgc ttgacatcga gatcgccacc taccgcaagc tgctggaggg cgaggagagc
     1201 aggctggagt ctgggatgca gaacatgagc atccacacga agaccaccag tggctacgca
     1261 ggaggactga gttcatccta cgggggactc actagccccg gcttcagcta tggaatgagc
     1321 tctttccagc ccggcttcgg ttctgttggg ggatccagca cttatagccg caccaaggct
     1381 gtggtcgtga agaagattga aacccgagat gggaaactgg tgtctgagtc ttctgacatc
     1441 atgtccaagt gaatggccac tgaagtcatt gccagcctga ggtcctgcag ctgctcaggg
     1501 gtcaagggga gacagctgta tggcagagtg cagggaacta gggaccagcc agagtaccag
     1561 ccctaaacct ctggccaacc ttgggaggaa tttcctatct gggatatgcc aatgcccaac
     1621 tcaattgtat tttccaaaat aaagcctcag ctggctgtat caactaaaaa aaaaaaaaaa
     1681 aaaaaaaaaa aaaaa
//
