LOCUS       XM_232840               6746 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus kelch-like family member 32 (Klhl32),
            transcript variant X1, mRNA.
ACCESSION   XM_232840
VERSION     XM_232840.10
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051340.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Feb 22, 2024 this sequence was replaced by
            XM_232840.11.
            
            On Jan 21, 2021 this sequence version replaced XM_232840.9.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..6746
                     /gene="Klhl32"
                     /note="kelch-like family member 32; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 20
                     ESTs, 13 Proteins, and 100% coverage of the annotated
                     genomic feature by RNAseq alignments, including 80 samples
                     with support for all annotated introns"
                     /db_xref="GeneID:313111"
                     /db_xref="RGD:1310364"
     CDS             441..2303
                     /gene="Klhl32"
                     /codon_start=1
                     /product="kelch-like protein 32 isoform X1"
                     /protein_id="XP_232840.4"
                     /db_xref="GeneID:313111"
                     /db_xref="RGD:1310364"
                     /translation="MPSEHCLSIQEMLTGQRLCHSESHNDTVLAALNQQRSDGILCDV
                     TLIAEEQKFHAHKAVLAACSDYFRAMFSLCMVESGADEVNLRGVTSLGLKQALEFAYT
                     GQILLEPGVIQDVLAAGSHLQLLELLNLCSHYLIQELNSFNYLDLYRLADLFHLTLLE
                     KAVIDFLVKHLSELLKSRPEDVLTLPYCLLQEVLKSDRLTSLSEEQIWQLAVRWLEHN
                     CHYQHMDELLQYIRFGLMDVDTLHTVALSHPLVQASETATALVNEALEYHQSIYAQPV
                     WQTRRTKPRFQSDTLYIIGGKKREVCKVKELRYFNPVDQENALIAAIANWSELAPMPV
                     GRSHHCVAVMGDFLFVAGGEVEHASGRTCAVRTACRYDPRSNSWAEIAPMKNCREHFV
                     LGAMDEYLYAVGGRNELRQVLPTVERYCPKKNKWTFVQSFDRSLSCHAGYVADGLLWI
                     SGGVTNTAQYQNRLMAYEPKQNKWISRSPMLQRRVYHSMAAVQRKLYVLGGNDLDYNN
                     DRILVRHIDSYNIDTDQWTRCNFNLLTGQNESGVAVHNGRIYLVGGYSIWTNEPLACI
                     QVLDVSREGKEEVFYGPTLPFASNGIAACFLPAPYFTCPNLQTLQVPHHRIGTV"
ORIGIN      
        1 ggcgggagcg cgcagcggca ggagggcgga gcggctgcga ggggcggctg gaggccggct
       61 gctgggaggg tagaacgagc cgggttgcac gcgccacctg cgaggctggc tggctttctc
      121 tttccgcgga cacatccccg tccctccccc cagtcccctg gaggtcggga gcgcgcgagt
      181 tagcgcgcac acccgctcgc atccacacac acgccagagc gcgcgatcat cactgctgca
      241 gcccgggccg ggcagacaga gctgctgcag tttccccagg gtcgagcggg gaaagcagag
      301 ccgtcttatc ccggagagag cctggtgccc gccggccccc tggggtatca agcccagcag
      361 actgctcccc tccttcggct ggaatgcttg cggattcttc tgaaaccttc taagcctgag
      421 tctgaggaac cagctggagg atgccgtctg aacactgcct cagtattcaa gaaatgctga
      481 ctggccagag gctgtgccac tcggaatctc acaatgacac tgtcctcgca gcactgaatc
      541 agcaaagaag tgatggcatt ctctgcgacg tgaccctgat cgccgaggaa cagaaattcc
      601 atgctcacaa ggcagtccta gcggcctgca gtgactattt ccgggcaatg ttcagtcttt
      661 gtatggtgga aagtggagct gatgaagtaa atttgcgtgg tgtgaccagc ttgggcttaa
      721 aacaggctct ggagtttgca tacacaggac agattttgtt ggagccagga gtgatccagg
      781 atgtgttagc agctggcagc catctacagc tgttggaact tctcaatttg tgttcccact
      841 atctcatcca ggaactaaat agctttaatt acttggatct gtacagactt gctgacctct
      901 ttcacctcac tttgttggag aaggcagtga ttgatttcct agtgaagcat ctctcagagc
      961 ttctgaagag ccgcccagag gatgttctca cgctgcccta ctgcctgctg caggaggtgc
     1021 tgaagagtga ccgcttgacc tccctgagtg aggagcagat ctggcagcta gctgtgaggt
     1081 ggctggaaca caactgccat taccagcaca tggacgagct cctgcagtac atccgcttcg
     1141 gcctaatgga cgtggataca ctccacacag ttgccctatc ccaccccctg gtccaggcaa
     1201 gcgagacggc aactgccctt gtcaacgagg ccctggaata ccaccaaagc atctatgcac
     1261 agcctgtgtg gcagacccgc aggacaaaac cgcgattcca gtcagatact ctctatatta
     1321 ttggtgggaa aaagcgtgag gtctgcaaag tcaaggaact tcggtacttc aatcccgtgg
     1381 accaggagaa tgctctcatc gccgccattg ccaactggag tgagctggct cccatgccgg
     1441 tgggaaggag ccatcactgt gtggcagtca tgggggactt cctgtttgta gcaggaggag
     1501 aagtggagca tgccagtggc cggacatgtg ccgtgaggac tgcctgtcgc tatgaccccc
     1561 gcagtaattc ctgggcagag atagcaccca tgaaaaactg ccgggaacat tttgtgctgg
     1621 gtgccatgga tgaatacctc tatgcagtag ggggcagaaa tgaactgcgc caagttctac
     1681 ctacagttga gcgatactgc cccaagaaga acaagtggac gtttgtgcag tcctttgaca
     1741 gatccctgtc atgtcacgct ggatatgtgg ctgatggtct tctttggata tcaggtgggg
     1801 tgaccaacac agcgcagtat cagaacagac tgatggcgta cgagcctaag cagaataagt
     1861 ggataagtcg cagccctatg ctacagagaa gggtctacca ttccatggct gctgtccaga
     1921 ggaagcttta cgttcttgga ggcaatgacc tagattacaa taatgaccga attcttgtac
     1981 gtcatataga ctcttacaac attgacactg atcagtggac acgctgtaat ttcaacttgt
     2041 taactggcca gaatgaatct ggagttgctg tccataatgg gagaatatat ttagttggtg
     2101 gatattcaat ttggacaaat gagcctctgg cttgtatcca ggtcttggat gtaagcagag
     2161 aaggcaaaga ggaggtgttc tacggaccta cacttccctt tgcctcgaac ggaatagcag
     2221 cgtgtttcct tcccgctccg tatttcacgt gccctaacct tcagaccctg caagtgcctc
     2281 accacaggat cggcacggtc tgagaagcca gcgctccgtt agagcgcgtc accaggagac
     2341 cgagtgagac cctgacattt ggatgcaggg cgtgaacatc ctcagtgctc gatgcttccc
     2401 tgtcttgtct tacaggtcta aaattgtgct aagtactagc aaaaaaaaaa aaaaaaaaaa
     2461 aaagaataat tttctaaacc ctattatgga gaactttgtc acttctttca gtgtacgcga
     2521 acatacacta tgtatgactt ttgttgcggt gcaccttagt gccgtcctat ggcacgttat
     2581 tgttttgtgg atctttagaa cattctttgt acccctgtcc tacttagcgc cgtctgggat
     2641 catgtgacac cgtcagtgag gcaccttacg cccatggcat cagttttctc gtagtcttct
     2701 gtacttacgc ctcggtatta cttaacattc tgcagctccg ctcagaaggg tctcagcaga
     2761 atgacatcat atcagcttta agtgccactt ttccttcaaa gtgacaggag taaaggactt
     2821 ggaaacgtta gcttttccac cttggttact ctgacgttgt atcagaactc tatagggcta
     2881 ggtttctcac cgttggaaga cgataattac tcattcccgc attaactaaa aaaaaatgcc
     2941 gtgaggatta ggccaacgct cagaactttg cctaagggca aaaaaaaaaa aagttacttc
     3001 agctttaaag cgtttgtctg ctgcctctag agcccgaaag ggaaccattt tatttccaag
     3061 tttataactt ccgagatgga atgttgagaa ggtcctgtgg gatagaggag tctgagagca
     3121 tggggaaggg actgcaccca tcctgagcag gaacgccaaa gatcccaaaa cctggacaga
     3181 ttcttgcctc aaagaattcc ctttaattgt ctcacctaaa gttttttttt tttttttttt
     3241 ttttttaatt accggtacta accatggcac catagaaggc tgtgacctaa gtctgaactg
     3301 acagccagaa gttctcatag acattttcca agttgataga ggtagaagtc ttgcatactt
     3361 cgcggattct ccgccactgc caatacattt ttttaaatta ttactccttt caataatgaa
     3421 taattctttt ggtagtctag agttttgggc ttgctctata tcaaactaga aagaaataat
     3481 cttaataatc gcatcttaat atacatccaa catgtttatt tttttctaat cttgccaaga
     3541 cttgaggtca tttttattaa aagggaagct ccttcctctt agccgtttag gtcatttgct
     3601 tacgtcacag aaactgtcgt tttattcatg taaccactta ttttttagca tcataagtaa
     3661 atgaaaaaaa tgttttttct tcttgttcag gatggccttc cattggctat gtagctacgc
     3721 tggcctccaa ctcatgaata aaataatctt tgcaccttaa ctaatttact agttttatgc
     3781 tttgctgtat gatccctaaa gcactgttct gcaaatataa atgatctctg gttacctggg
     3841 ttaaaggaca ggcagtgata gagagggtaa ggaagaaatg taaaagatag gagttcagac
     3901 tgaaccaagg accacttgga cgagtgatgc gggtaagtga tgagaacatt ttaaaaactg
     3961 tctgtgagcc ttcagagata aacatgactg acctgtgagg aaagtgcctt cctgcctttc
     4021 tgatcttcaa cacggagcta cataagaaat acgggatttc tccttgatac atgaattttt
     4081 cctctggtag ttttgcgttg taaaaccatt aacttctatt tatgttctcg ggtcctacac
     4141 ccagggagtg taaggaatgt cttgaaaata gcctgcctga cagataatgg acataaccct
     4201 agactattct gtatccattt ctaaccattc accaaagtca gcattaatta ccagtttctc
     4261 cttgatgttc ttcatgggac actttctgta gtttggatag attcctcaca tctataatca
     4321 aagaacggca tcaaaagtat cacagtccct tactaaggag aaaaaaatga ttgtataatt
     4381 ggatcccttt ccttgaaatc atctgcaatg agaaagaaat ctgtgaaatt gtatttggtg
     4441 tatagacaca aatgctaccc agctctgtgt ctactattta agtgtagtat cctatggtaa
     4501 cttatctttc attttcagtc tttaaaatta aagactatga tttcctttcc atttgagacc
     4561 ataagacttt gacacatgta aacatcaagt aaggttttta ctttaaaaca caaacaaaca
     4621 aaccaaaata agacaaaaaa agaagttccg attgttctgc tgtgagagaa gctagcaaag
     4681 acacagcatc catctacaat gccagatgca gcaaacccct taatctctaa aataaaatgc
     4741 ccactcagct acagttgtgg cactcctgtg ccttctcatt tttcttatac ctgatgtaca
     4801 tgtgtagcat ccccttcagc tcttgcaaaa gtcgaacccc tttgttgttt tgaacaggaa
     4861 gtctaaatgc agtaggaaga ggacagatac tgtgtcacta agtgacatgg ctcgccttta
     4921 ctattagtgt atattccctg gcacaatgta acttaaacga ctgcatcaca ttttatatac
     4981 ctatctcacc gtcattatct gcaaacaacc tatttatcac ctataaacta atactatgat
     5041 gtaataaacg gtagttatca gcacaacaga gttctaagtg aggactgggg ttgctgttca
     5101 gctgaaacag tgttcgtctt gccggcagga agcctggctt cccatctcta gtgcactaac
     5161 ctggctgtgc tggctcatgt ctgtaatccc ggtaggcttg ggaagtcctg gctaccaaga
     5221 gagttcgaag aagccagcct gcaaacagag attccaaccc tgtctttcag agccaaatac
     5281 aataataaag ttggtaaatt ataagcatgt ggtattgaat tgagtaacct cagcattgca
     5341 gatgagcaca gtaaaaacac aactcttgtg tctaatcaca cataggaaag aatatgcttt
     5401 ttttttcttt taagaaatct agaccgactg ctttaaccga atcaagcaca ttgctctgac
     5461 tgccacttta cactcctgct tgactaggca atacatttaa ctctaagaag ggtgggcatt
     5521 tagttttgct cagtacagaa aagatgtagg acttagagaa gagctccttc agagtttccc
     5581 aaaatgcaga gacatatcaa tttctacaga aaatcttact tgtcaaagat tctataagaa
     5641 ctgagtgtgg ttactgtaat ggtgagcacc tgacacacca tcattccagc acttggtgag
     5701 agggaagctg aggcagggtg attttatggc ctaggatgct taggacacac acacacacac
     5761 acacacacac acacacacac acacacacac acacacaata tacatgtaca aacaacattt
     5821 aaaagaaatc ttttattgaa aagaataaaa ccagtatggg acatgcacct ataatcctat
     5881 cactggggtg tctaggagga ttttgagttt gagtctggcc tgagctatgg tgagccgaaa
     5941 atcatacagt cagactctgc ctcaacaaaa caaaatcacc aagaaaacaa tgcactaatc
     6001 aaaactccag caaacttgtg aaaaggaatg gtagaagcta caggataata aaaatcaagt
     6061 aatctaaaac ataatactat tttcttaaat gcttaaatag aaaaccaatc aaatgcagtc
     6121 atctctcatt taatggctgg aatgtattct gagaaatgcg tccttaggca aaatcactgt
     6181 gcttacacaa taggtggtag tcaatcacac ttgtggcctc tggatgctat ccagatttgt
     6241 ggtgctggtg taacaaggca tactgtttta cagttagttt tttttctgag tacttttata
     6301 cattaggaat ataatctaaa atgaggggaa gtaaatacac aaagcaccaa gatatcaacc
     6361 aggaagtatt acgcagtgtg catagatgta tagttctgtg tctagcaggg cagtgggctt
     6421 acctgtgcta gcatccgtcc atgtgctgta tatgttatac tgtgatgttg caatggctgt
     6481 gcacctaagt gttagaaact tcatagctct gctatgatct cctggtatac cttggtatgt
     6541 gtttcttaaa catcatgcac cataatgttt tacttaagaa aaaaaagaaa agaaaggtaa
     6601 agatacagat gtttaaaatt atgtaaccaa tactgatttt tgcagatgat cctccaaatt
     6661 tttcttaccc atggaaacaa tctagttaat taaattaaag tttaaatcac agccaaatct
     6721 taatgtctaa taaaacattt tcatca
//
