LOCUS       NM_031336               3527 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Rattus norvegicus Klotho (Kl), mRNA.
ACCESSION   NM_031336
VERSION     NM_031336.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Cararo-Lopes,M.M., Sadovnik,R., Fu,A., Suresh,S., Gandu,S. and
            Firestein,B.L.
  TITLE     Overexpression of alpha-Klotho isoforms promotes distinct Effects
            on BDNF-Induced Alterations in Dendritic Morphology
  JOURNAL   Mol Neurobiol 61 (11), 9155-9170 (2024)
   PUBMED   38589756
  REMARK    GeneRIF: Overexpression of alpha-Klotho isoforms promotes distinct
            Effects on BDNF-Induced Alterations in Dendritic Morphology.
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Qiu,Z., Qi,B., Li,L., Cui,J., Liu,M. and Xia,Z.
  TITLE     ACTIVATION OF KLOTHO/SIRT1 SIGNALING PATHWAY ATTENUATES MYOCARDIAL
            ISCHEMIA REPERFUSION INJURY IN DIABETIC RATS
  JOURNAL   Shock 62 (3), 447-456 (2024)
   PUBMED   38904464
  REMARK    GeneRIF: ACTIVATION OF KLOTHO/SIRT1 SIGNALING PATHWAY ATTENUATES
            MYOCARDIAL ISCHEMIA REPERFUSION INJURY IN DIABETIC RATS.
REFERENCE   3  (bases 1 to 3527)
  AUTHORS   Yuan,X.K., Ni,P.S., Yan,Z.H., Yu,Z., Wang,Z.Z., Zhang,C.K., Li,F.H.
            and Yu,X.M.
  TITLE     Effects of Life-Long Exercise on Age-Related Inflammation,
            Apoptosis, Oxidative Stress, Ferroptosis Markers, and NRF2/KAEP
            1/Klotho Pathway in Rat Kidneys
  JOURNAL   Physiol Res 73 (4), 577-591 (2024)
   PUBMED   39264079
  REMARK    GeneRIF: Effects of Life-Long Exercise on Age-Related Inflammation,
            Apoptosis, Oxidative Stress, Ferroptosis Markers, and NRF2/KAEP
            1/Klotho Pathway in Rat Kidneys.
REFERENCE   4  (bases 1 to 3527)
  AUTHORS   Kale,A., Shelke,V., Habshi,T., Dagar,N. and Gaikwad,A.B.
  TITLE     ER stress modulated Klotho restoration: A prophylactic therapeutic
            strategy against acute kidney injury-diabetes comorbidity
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (1), 166905 (2024)
   PUBMED   37793463
  REMARK    GeneRIF: ER stress modulated Klotho restoration: A prophylactic
            therapeutic strategy against acute kidney injury-diabetes
            comorbidity.
REFERENCE   5  (bases 1 to 3527)
  AUTHORS   Wang,H., Liu,H., Cheng,H., Xue,X., Ge,Y., Wang,X. and Yuan,J.
  TITLE     Klotho Stabilizes the Podocyte Actin Cytoskeleton in Idiopathic
            Membranous Nephropathy through Regulating the TRPC6/CatL Pathway
  JOURNAL   Am J Nephrol 55 (3), 345-360 (2024)
   PUBMED   38330925
  REMARK    GeneRIF: Klotho Stabilizes the Podocyte Actin Cytoskeleton in
            Idiopathic Membranous Nephropathy through Regulating the TRPC6/CatL
            Pathway.
REFERENCE   6  (bases 1 to 3527)
  AUTHORS   Nagai,R., Saito,Y., Ohyama,Y., Aizawa,H., Suga,T., Nakamura,T.,
            Kurabayashi,M. and Kuroo,M.
  TITLE     Endothelial dysfunction in the klotho mouse and downregulation of
            klotho gene expression in various animal models of vascular and
            metabolic diseases
  JOURNAL   Cell Mol Life Sci 57 (5), 738-746 (2000)
   PUBMED   10892340
REFERENCE   7  (bases 1 to 3527)
  AUTHORS   Kato,Y., Arakawa,E., Kinoshita,S., Shirai,A., Furuya,A., Yamano,K.,
            Nakamura,K., Iida,A., Anazawa,H., Koh,N., Iwano,A., Imura,A.,
            Fujimori,T., Kuro-o,M., Hanai,N., Takeshige,K. and Nabeshima,Y.
  TITLE     Establishment of the anti-Klotho monoclonal antibodies and
            detection of Klotho protein in kidneys
  JOURNAL   Biochem Biophys Res Commun 267 (2), 597-602 (2000)
   PUBMED   10631108
REFERENCE   8  (bases 1 to 3527)
  AUTHORS   Ohyama,Y., Kurabayashi,M., Masuda,H., Nakamura,T., Aihara,Y.,
            Kaname,T., Suga,T., Arai,M., Aizawa,H., Matsumura,Y., Kuro-o,M.,
            Nabeshima,Yi. and Nagail,R.
  TITLE     Molecular cloning of rat klotho cDNA: markedly decreased expression
            of klotho by acute inflammatory stress
  JOURNAL   Biochem Biophys Res Commun 251 (3), 920-925 (1998)
   PUBMED   9791011
REFERENCE   9  (bases 1 to 3527)
  AUTHORS   Aizawa,H., Saito,Y., Nakamura,T., Inoue,M., Imanari,T., Ohyama,Y.,
            Matsumura,Y., Masuda,H., Oba,S., Mise,N., Kimura,K., Hasegawa,A.,
            Kurabayashi,M., Kuro-o,M., Nabeshima,Y. and Nagai,R.
  TITLE     Downregulation of the Klotho gene in the kidney under sustained
            circulatory stress in rats
  JOURNAL   Biochem Biophys Res Commun 249 (3), 865-871 (1998)
   PUBMED   9731228
REFERENCE   10 (bases 1 to 3527)
  AUTHORS   Kuro-o,M., Matsumura,Y., Aizawa,H., Kawaguchi,H., Suga,T.,
            Utsugi,T., Ohyama,Y., Kurabayashi,M., Kaname,T., Kume,E.,
            Iwasaki,H., Iida,A., Shiraki-Iida,T., Nishikawa,S., Nagai,R. and
            Nabeshima,Y.I.
  TITLE     Mutation of the mouse klotho gene leads to a syndrome resembling
            ageing
  JOURNAL   Nature 390 (6655), 45-51 (1997)
   PUBMED   9363890
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000012.1.
            
            On Nov 27, 2020 this sequence version replaced NM_031336.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB017820.1, SRR26643287.27536.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-840               JAXUCZ010000012.1  5326003-5326842
            841-1351            JAXUCZ010000012.1  5356774-5357284
            1352-1620           JAXUCZ010000012.1  5358535-5358803
            1621-2722           JAXUCZ010000012.1  5362368-5363469
            2723-3527           JAXUCZ010000012.1  5364875-5365679
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12p12"
     gene            1..3527
                     /gene="Kl"
                     /note="Klotho"
                     /db_xref="GeneID:83504"
                     /db_xref="RGD:620396"
     exon            1..840
                     /gene="Kl"
                     /inference="alignment:Splign:2.1.0"
     CDS             16..3060
                     /gene="Kl"
                     /EC_number="3.2.1.31"
                     /codon_start=1
                     /product="klotho precursor"
                     /protein_id="NP_112626.1"
                     /db_xref="GeneID:83504"
                     /db_xref="RGD:620396"
                     /translation="MPARAPPRRLPRLLLLRLLSLHLLLLTLRARCLSAEPGQGAQTW
                     ARFARPPVPEASGLLHDTFPDGFLWAVGSAAYQTEGGWRQHGKGASIWDTFTHHPRAI
                     PEDSPIVMAPSGAPLPPLPSTGDVASDSYNNVYRDTEGLRELGVTHYRFSISWARVLP
                     NGTAGTPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDTYGGWANRALADHF
                     RDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGVRGSSRLGYLVAHNLLLA
                     HAKVWRLYNTSFRPTQGGRVSIALGSHWITPRRMTDYHIRECQKSLDFVLGWFAKPIF
                     IDGDYPKSMKNNLSSLLPDFTESEKRFIRGTADFFALSFGPTLSFQLLDPSMKFRQLE
                     SPSLRQLLSWIDLEYNHPQIFIVENGWFVSGTTRRDDAKYMYYLKKFIMESLKAIRLD
                     GVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKELLPKSSALFYQKLIENNGF
                     PPLPENQPLEGTFPCDFAWGVVDNYIQVDPTLSQFTDPNVYLWDVHHSKRLIKVDGVV
                     AKKRKPYCVDFSAIRPQITLLREMRVTHFRFSLDWALILPLGNQTQVNRTVLHFYRCM
                     VSELVHANITPVVALWQPATPHQGLPHALAKHGAWENPHTALAFADYANLCFEELGHW
                     VKFWITINEPNSRNMTYRAGHHLLKAHALAWHLYDDKFRAAQKGKISIALQVDWIEPA
                     CPFSQKDKEVAERVLEFDVGWLAEPIFGSGDYPHVMREWLNQKNNFLLPYFTEDEKKL
                     IRGSFDFLALSHYTTILVDWEKEDPIKYNDYLEVQEMTDITWLNSPNQVAVVPWGLRK
                     ALNWLRFKYGDLPMFVTANGIDDDPHAEQDSLRMYYIKNYVNEALKAYVLDGINLCGY
                     FAYSLSDRSVPKSGFYRYAANQFEPKPSIKHYRKIIDNNGFLGSGTLGRFCPEEYTVC
                     TGCGFFQTRKSLLAFISFLVFAFVTSLALIYYYSKKGRRRYK"
     exon            841..1351
                     /gene="Kl"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..1620
                     /gene="Kl"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..2722
                     /gene="Kl"
                     /inference="alignment:Splign:2.1.0"
     exon            2723..3527
                     /gene="Kl"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cccggctccc gcagcatgcc agcccgcgcc cctcctcgcc gcctgccgcg gctcttgctg
       61 ctccgtttgc tgtcgctgca tctgctgttg ctcaccctgc gcgcccgctg cctaagcgct
      121 gagccgggtc agggcgcgca gacctgggct cgctttgcgc gccctcctgt cccagaggcc
      181 tctggcctcc tccatgacac cttccccgac ggtttcctct gggcggtagg cagcgccgcc
      241 taccagacgg agggcggctg gcgacagcac ggcaaaggcg cgtccatctg ggacactttc
      301 acccatcacc ctcgggccat cccggaggac tccccgatcg tcatggcgcc gtcgggtgcc
      361 ccgttgcctc ccctgccctc cactggagat gtggccagcg acagttacaa caacgtctac
      421 cgcgacacag aggggctacg cgaactgggg gtcacccact accgcttctc catatcgtgg
      481 gcgcgggtgc tccccaatgg caccgcgggc acccccaacc gcgaggggct gcgctactac
      541 cggcggctgc tggagcggct gagggagctg ggcgtgcagc cggtggtcac tctgtaccat
      601 tgggacctgc cgcagcgcct gcaggacacc tatggcggct gggccaatcg cgccctggcc
      661 gaccatttca gggattacgc cgagctctgc ttccgccact tcggtggtca ggtcaagtac
      721 tggatcacca ttgacaaccc ctacgtggtg gcctggcacg ggtacgccac cggccgcctg
      781 gccccgggcg tgcggggcag ctccaggctc gggtacctgg ttgcccacaa cctacttttg
      841 gctcacgcca aagtctggcg tctctacaac acctccttcc gccccactca ggggggccgg
      901 gtgtccatcg ccttaggttc ccattggatc acccctcgaa ggatgaccga ctatcacatc
      961 agagagtgtc agaagtctct tgacttcgtg ctaggctggt ttgccaagcc catatttatc
     1021 gatggcgact acccaaagag tatgaagaac aacctctcat ccctgctgcc cgattttact
     1081 gaatccgaga agagattcat caggggaaca gctgacttct ttgctctctc cttcggacca
     1141 accttgagct ttcaactact ggaccccagc atgaagttcc gccagctgga gtctcccagc
     1201 ctgaggcagc ttctgtcttg gatagatctg gagtataacc accctcagat atttattgta
     1261 gaaaatggct ggtttgtctc ggggaccacc agaagagatg atgccaaata catgtattat
     1321 ctcaagaagt tcataatgga aagcttaaaa gccatcaggc tggatggggt cgacgtcatt
     1381 gggtacaccg catggtccct catggatggt ttcgagtggc acaggggcta cagcatccga
     1441 cgaggactct tctatgtcga ctttctgagt caggacaagg agttgttgcc caagtcttcg
     1501 gccttgttct accaaaagct gatagagaac aatggcttcc ctcctttacc tgagaaccag
     1561 ccccttgaag ggacgtttcc ctgtgacttt gcttggggag tggttgacaa ctacattcaa
     1621 gtggacccca ctctctctca gtttactgac ccgaatgtct atctgtggga tgtgcatcac
     1681 agtaagaggc ttattaaagt agacggggtt gtagccaaga agagaaaacc ttactgtgtt
     1741 gatttctctg ccatccggcc tcagataacc ttacttcgag aaatgcgggt cacccacttt
     1801 cggttctccc tagactgggc cctgatcttg cctctgggta accagactca agtgaaccgc
     1861 acggttctgc acttctaccg ctgtatggtc agcgagctgg tgcacgccaa catcactcca
     1921 gtggtggccc tgtggcaacc agcaacccca caccaaggcc tgccacatgc ccttgcaaaa
     1981 catggggcct gggagaaccc gcacactgcc ctggcatttg cagactacgc aaacctgtgc
     2041 tttgaagagc taggccactg ggtcaagttc tggatcacca taaatgagcc aaactcacgg
     2101 aacatgacat accgtgcggg gcaccacctg cttaaggctc atgccttggc atggcacctg
     2161 tacgatgaca aatttagggc ggctcagaaa ggcaaaatat ccatcgcctt gcaggttgac
     2221 tggatagaac cggcctgccc attctctcag aaggacaaag aagtggcgga gagagttttg
     2281 gaatttgatg taggctggct ggcagagccc attttcggct ccggagatta tccgcatgtg
     2341 atgagggaat ggctgaacca aaaaaacaac tttcttctgc cctatttcac ggaagatgag
     2401 aaaaagctaa tccggggttc ctttgacttc ctggcgttga gccattacac caccatcctt
     2461 gtagactggg aaaaggagga tccgataaaa tacaacgatt acctggaggt gcaggagatg
     2521 actgacatca cgtggctcaa ctcccccaat caggtggcgg tggtgccttg ggggctgcgc
     2581 aaagcgctca actggctaag gttcaagtat ggagacctcc cgatgttcgt gacagccaat
     2641 ggcattgatg acgatcccca tgccgagcaa gactcactga ggatgtatta tattaagaat
     2701 tacgtgaatg aggctctgaa agcctacgtg ttggacggca tcaacctttg tggctacttt
     2761 gcgtattcgc ttagtgaccg ctcagttccc aagtccggct tttatcgata cgctgcgaat
     2821 cagttcgagc ccaaaccgtc tattaaacat tacaggaaaa ttattgacaa caacggcttc
     2881 ctgggttctg gaacgctggg aaggttttgt ccggaagagt acaccgtgtg caccggatgc
     2941 ggcttttttc aaacccggaa gtccctgctg gccttcatct cgtttctagt ttttgctttc
     3001 gttacttctc tcgctcttat ttattactac tccaagaaag gccggagacg ttataaataa
     3061 gtgtgaacgc ccgccctgcc attcgctttg ggatcagtac gcacgcaccg tcaaccgtca
     3121 gctgcttgtg ttgtgatgcc acgttccaca cttttggact ctggaaaacc ttttcctgtc
     3181 aaggatggtg gcggttttaa acaggcactg gcgactataa aatattgcag ggtgaatggt
     3241 atctgaatct gctctctttg gcggcaatta cgcacctatg cccaccgcag tttctacact
     3301 gtgggagtac acagtagggt caccgaagtt tctacagcac ccccgtaagg aagacacgga
     3361 atatgcttgt ggtaacagtg cctttgggta aagacaggac tttgaagagc cactccagga
     3421 acttttctat cagtgaccat tttgaaattc acatcttctt tggaagttac agtcactcag
     3481 ttggcgtctt aacacctgga ctttacctga tccagtttta caagggg
//
