LOCUS       NM_053795               7135 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus kinase D-interacting substrate 220 (Kidins220),
            mRNA.
ACCESSION   NM_053795
VERSION     NM_053795.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Lopez-Menendez,C., Simon-Garcia,A., Gamir-Morralla,A.,
            Pose-Utrilla,J., Lujan,R., Mochizuki,N., Diaz-Guerra,M. and
            Iglesias,T.
  TITLE     Excitotoxic targeting of Kidins220 to the Golgi apparatus precedes
            calpain cleavage of Rap1-activation complexes
  JOURNAL   Cell Death Dis 10 (7), 535 (2019)
   PUBMED   31296845
  REMARK    GeneRIF: Excitotoxicity induces Kidins220 and GluN1 traffic to the
            Golgi apparatus before Kidins220 is degraded by the protease
            calpain. Excitotoxicity also triggers an early activation of
            Rap1-GTPase followed by its inactivation. Kidins220 excitotoxic
            endocytosis and subsequent calpain-mediated downregulation governs
            inactivation of Rap1 that is associated to decreases in ERK
            activity preceding neuronal death.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Josifova,D.J., Monroe,G.R., Tessadori,F., de Graaff,E., van der
            Zwaag,B., Mehta,S.G., Harakalova,M., Duran,K.J., Savelberg,S.M.,
            Nijman,I.J., Jungbluth,H., Hoogenraad,C.C., Bakkers,J.,
            Knoers,N.V., Firth,H.V., Beales,P.L., van Haaften,G. and van
            Haelst,M.M.
  CONSRTM   DDD Study
  TITLE     Heterozygous KIDINS220/ARMS nonsense variants cause spastic
            paraplegia, intellectual disability, nystagmus, and obesity
  JOURNAL   Hum Mol Genet 25 (11), 2158-2167 (2016)
   PUBMED   27005418
  REMARK    GeneRIF: This work reveals a crucial physiological role of
            KIDINS220 in development and provides insight into how perturbation
            of the complex interplay of KIDINS220 isoforms and their relative
            expression can affect neuron control and human metabolism.
            Altogether, we here show that de novo protein-truncating KIDINS220
            variants cause a new syndrome, SINO. This is the first report of
            KIDINS220 variants causing a human disease.
REFERENCE   3  (bases 1 to 7135)
  AUTHORS   Lopez-Benito,S., Lillo,C., Hernandez-Hernandez,A., Chao,M.V. and
            Arevalo,J.C.
  TITLE     ARMS/Kidins220 and synembryn-B levels regulate NGF-mediated
            secretion
  JOURNAL   J Cell Sci 129 (9), 1866-1877 (2016)
   PUBMED   26966186
  REMARK    GeneRIF: overexpression of ARMS blocked NGF-mediated secretion,
            without affecting basal secretion, a decrease in ARMS resulted in
            potentiation. Similar effects were observed with synembryn-B, a
            protein that interacts directly with ARMS. Downstream of ARMS and
            synembryn-B are Galphaq and Trio proteins, which modulate the
            activity of Rac1 in response to NGF
REFERENCE   4  (bases 1 to 7135)
  AUTHORS   Schmieg,N., Thomas,C., Yabe,A., Lynch,D.S., Iglesias,T.,
            Chakravarty,P. and Schiavo,G.
  TITLE     Novel Kidins220/ARMS Splice Isoforms: Potential Specific Regulators
            of Neuronal and Cardiovascular Development
  JOURNAL   PLoS One 10 (6), e0129944 (2015)
   PUBMED   26083449
  REMARK    GeneRIF: the existence of novel Kidins220/ARMS splice isoforms with
            unique properties
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7135)
  AUTHORS   Andreazzoli,M., Gestri,G., Landi,E., D'Orsi,B., Barilari,M.,
            Iervolino,A., Vitiello,M., Wilson,S.W. and Dente,L.
  TITLE     Kidins220/ARMS interacts with Pdzrn3, a protein containing multiple
            binding domains
  JOURNAL   Biochimie 94 (9), 2054-2057 (2012)
   PUBMED   22609016
  REMARK    GeneRIF: a novel partner of Kidins220/ARMS
REFERENCE   6  (bases 1 to 7135)
  AUTHORS   Luo,S., Chen,Y., Lai,K.O., Arevalo,J.C., Froehner,S.C., Adams,M.E.,
            Chao,M.V. and Ip,N.Y.
  TITLE     {alpha}-Syntrophin regulates ARMS localization at the neuromuscular
            junction and enhances EphA4 signaling in an ARMS-dependent manner
  JOURNAL   J Cell Biol 169 (5), 813-824 (2005)
   PUBMED   15939763
  REMARK    GeneRIF: Altogether, our findings suggest that ARMS may play an
            important role in regulating postsynaptic signal transduction
            through the syntrophin-mediated localization of receptor tyrosine
            kinases such as EphA4.
REFERENCE   7  (bases 1 to 7135)
  AUTHORS   Cabrera-Poch,N., Sanchez-Ruiloba,L., Rodriguez-Martinez,M. and
            Iglesias,T.
  TITLE     Lipid raft disruption triggers protein kinase C and Src-dependent
            protein kinase D activation and Kidins220 phosphorylation in
            neuronal cells
  JOURNAL   J Biol Chem 279 (27), 28592-28602 (2004)
   PUBMED   15096499
  REMARK    GeneRIF: Lipid raft disruption triggers protein kinase C and
            Src-dependent protein kinase D activation and Kidins220
            phosphorylation in neuronal cells
REFERENCE   8  (bases 1 to 7135)
  AUTHORS   Arevalo,J.C., Yano,H., Teng,K.K. and Chao,M.V.
  TITLE     A unique pathway for sustained neurotrophin signaling through an
            ankyrin-rich membrane-spanning protein
  JOURNAL   EMBO J 23 (12), 2358-2368 (2004)
   PUBMED   15167895
REFERENCE   9  (bases 1 to 7135)
  AUTHORS   Kong,H., Boulter,J., Weber,J.L., Lai,C. and Chao,M.V.
  TITLE     An evolutionarily conserved transmembrane protein that is a novel
            downstream target of neurotrophin and ephrin receptors
  JOURNAL   J Neurosci 21 (1), 176-185 (2001)
   PUBMED   11150334
REFERENCE   10 (bases 1 to 7135)
  AUTHORS   Iglesias,T., Cabrera-Poch,N., Mitchell,M.P., Naven,T.J.,
            Rozengurt,E. and Schiavo,G.
  TITLE     Identification and cloning of Kidins220, a novel neuronal substrate
            of protein kinase D
  JOURNAL   J Biol Chem 275 (51), 40048-40056 (2000)
   PUBMED   10998417
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            On Nov 26, 2020 this sequence version replaced NM_053795.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: KR081256.1, AF239045.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-78                JAXUCZ010000006.1  47346907-47346984
            79-219              JAXUCZ010000006.1  47352626-47352766
            220-318             JAXUCZ010000006.1  47359686-47359784
            319-417             JAXUCZ010000006.1  47360979-47361077
            418-516             JAXUCZ010000006.1  47362351-47362449
            517-615             JAXUCZ010000006.1  47363099-47363197
            616-714             JAXUCZ010000006.1  47366482-47366580
            715-912             JAXUCZ010000006.1  47369485-47369682
            913-1011            JAXUCZ010000006.1  47373741-47373839
            1012-1110           JAXUCZ010000006.1  47375024-47375122
            1111-1209           JAXUCZ010000006.1  47375573-47375671
            1210-1387           JAXUCZ010000006.1  47378216-47378393
            1388-1552           JAXUCZ010000006.1  47379711-47379875
            1553-1732           JAXUCZ010000006.1  47380491-47380670
            1733-1898           JAXUCZ010000006.1  47381406-47381571
            1899-2050           JAXUCZ010000006.1  47384556-47384707
            2051-2340           JAXUCZ010000006.1  47384876-47385165
            2341-2481           JAXUCZ010000006.1  47386264-47386404
            2482-2725           JAXUCZ010000006.1  47387061-47387304
            2726-2814           JAXUCZ010000006.1  47387819-47387907
            2815-2959           JAXUCZ010000006.1  47389302-47389446
            2960-3122           JAXUCZ010000006.1  47392760-47392922
            3123-3301           JAXUCZ010000006.1  47409113-47409291
            3302-3525           JAXUCZ010000006.1  47411462-47411685
            3526-3639           JAXUCZ010000006.1  47413548-47413661
            3640-3672           JAXUCZ010000006.1  47420923-47420955
            3673-3804           JAXUCZ010000006.1  47423355-47423486
            3805-3903           JAXUCZ010000006.1  47425177-47425275
            3904-4140           JAXUCZ010000006.1  47426185-47426421
            4141-7135           JAXUCZ010000006.1  47428871-47431865
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q16"
     gene            1..7135
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /note="kinase D-interacting substrate 220"
                     /db_xref="GeneID:116478"
                     /db_xref="RGD:619949"
     exon            1..78
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            79..219
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..5400
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /note="kinase D-interacting substance of 220 kDa; kinase
                     D-interacting substance 220; ankyrin repeat-rich
                     membrane-spanning protein"
                     /codon_start=1
                     /product="kinase D-interacting substrate of 220 kDa"
                     /protein_id="NP_446247.2"
                     /db_xref="GeneID:116478"
                     /db_xref="RGD:619949"
                     /translation="MSVLISQSVINYVEEENIPALKALLEKCKDVDERNECGQTPLML
                     AAEQGNVEIVKELLKNGANCNLEDLDNWTALISASKEGHIHIVEELLKSGASLEHRDM
                     GGWTALMWACYKGRTDVVELLLSHGANPSVTGLYSVYPIIWAAGRGHADIVHLLLQNG
                     AKVNCSDKYGTTPLVWAARKGHLECVKHLLAMGADVDQEGANSMTALIVAVKGGYTQS
                     VKEILKRNPNVNLTDKDGNTALMIASKEGHIEIVQDLLDAGTYVNIPDRSGDTVLIGA
                     VRGGHVEIVRALLQKYADIDIRGQDNKTALYWAVEKGNATMVRDILQCNPDTEICTKD
                     GETPLIKATKMRNIEVVELLLDKGAKVSAVDKKGDTPLHVAIRGRSRRLAELLLRNPK
                     DGRLLYRPNKAGETPYNIDCSHQKSILTQIFGARHLSPTETDGDMLGYDLYSSALADI
                     LSEPTMQPPICVGLYAQWGSGKSFLLKKLEDEMKTFAGQQTEPLFQFSWLIVFLTLLL
                     CGGLGLVFAFTVDTNLAIAISLSFLALIYIFFIVIYFGGRREGESWNWAWALSTRLAR
                     HIGYLELLFKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDA
                     CEREFGFLATRLFRVFRTEESQGKKKWKKTCCLPSFVIFLFIVGCIIAGITLLAIFRV
                     DPKHLTVNAILISIASVVGLAFVLNCRTWWQVLDSLLNSQRKRLHSAASKLHKLKSEG
                     FMKVLKCEVELMARMAKTIDSFTQNQTRLVVIIDGLDACEQDKVLQMLDTVRVLFSKG
                     PFIAIFASDPHIIIKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLNSRGLSNARKFL
                     VTSATNGDITCSDTTGTQEDTDRRVSQNSLGEMTKLGSKTALNRRDTYRRRQMQRTIT
                     RQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQITFNWDRLASWINLT
                     EQWPYRTSWLILYLEETEGLPDQMTLKTIYERISKNIPTTKDVEPLLEIDGDIRNFEV
                     FLSSRTPVLVARDVKTFLPCTVNLDPKLREIIADVRAAREQINIGGLAYPPLPLHEGP
                     PRPPSGYSQPASVCSSASFNGPFPGGVVSPQPHSSYYSGLSGPQHPFYNRPFFAPYLY
                     TPRYYPGGSQHLISRSSVKTSLPRDQNNGLPCDSGFNKQRQATVPATGSSLLLSSMTV
                     DVVCEKLRQIEGLDQSMMPQYCTTIKKANINGRVLSQCNIDELKKEMAMNFGDWHLFR
                     SMVLEMRSVESQVVPEDPRFLNENSSAPVPHGESARRSSHTELPLTELSSQTPYTLNF
                     SFEELNTLGLDEGAPRHSNLSWQSQTRRTPSLSSLNSQDSSIEISKLTDKVQAEYRDA
                     YREYIAQMSQLEGGTGSSTISGRSSPHSTYYIGQSSSGGSIHSTLEQERGKEGELKQE
                     DGRKSFLMKRGDVIDYSSSGVSTNEASPLDPITEEDEKSDQSGSKLLPGKKSSERPSL
                     FQTDLKLKGGGLRYQKLPSDEDESGTEESDNTPLLKDDKDKKAEGKAERVCKSPEHSA
                     EPIRTFIKAKEYLSDALLDKKDSSDSGVRSNESSPNHSLHNEAADDSQLEKANLIELE
                     DEGHSGKRGMPHSLSGLQDPIIARMSICSEDKKSPSECSLIASSPEESWPACQKAYNL
                     NRTPSTVTLNNNTAPTNRANQNFDEIEGIRETSQVILRPGPSPNPTAVQNENLKSMAH
                     KRSQRSSYTRLSKDASELHAASSESTGFGEERESIL"
     exon            220..318
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            319..417
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            418..516
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            517..615
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            616..714
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            715..912
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1011
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1110
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1209
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1387
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1552
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1732
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..1898
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..2050
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            2051..2340
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            2341..2481
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            2482..2725
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            2726..2814
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            2815..2959
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            2960..3122
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            3123..3301
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            3302..3525
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            3526..3639
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            3640..3672
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            3673..3804
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            3805..3903
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            3904..4140
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
     exon            4141..7135
                     /gene="Kidins220"
                     /gene_synonym="ARMS"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtcctctgac aaggtctgcg gccctgtgag ctggctctac agagccgagg gcggagtcct
       61 cagccaggcc gagatacggt gaattaaagt cggcaataga tttctgtgaa aatgtcagtt
      121 cttatatcac agagtgtgat aaattatgtg gaagaagaga atattcctgc tctgaaagct
      181 cttcttgaga agtgcaaaga tgtagatgag aggaacgagt gtggccagac gccgctgatg
      241 ctggctgcgg agcagggcaa tgtggaaata gtgaaagaac tgttaaagaa tggagccaac
      301 tgcaatctgg aagatctgga taactggact gcccttatat cggcatctaa ggaggggcac
      361 atccacattg tggaggagct gctgaagagc ggggccagtc tggagcaccg agacatgggt
      421 ggatggacag ctctcatgtg ggcgtgctac aaaggcagga ccgacgtggt cgagctgctt
      481 ctttctcatg gtgccaaccc aagtgtcact ggtttgtata gtgtctaccc aatcatctgg
      541 gcagcgggca gaggccatgc cgacatagtg catcttctac tgcagaatgg tgcgaaagtc
      601 aattgctccg acaagtacgg aacaaccccc ttggtatggg ctgcccgaaa gggtcatttg
      661 gaatgtgtga agcacttgtt ggccatggga gctgatgttg atcaagaagg agccaattcg
      721 atgactgcac ttatcgtggc agtgaaagga ggctacacac agtccgtgaa agagattttg
      781 aaaaggaatc caaatgtgaa cctaactgac aaggatggaa acacagctct gatgatcgca
      841 tcgaaggagg gacatattga gattgtgcag gacctgctgg acgccggaac atacgtgaac
      901 atacctgaca ggagtgggga tactgtattg atcggcgctg tgagaggtgg tcatgttgaa
      961 attgttcgag cacttctcca aaaatatgct gatatagaca ttagagggca ggacaataag
     1021 accgcgctgt attgggccgt tgaaaaggga aatgcgacaa tggtgaggga tatcctgcag
     1081 tgcaatccgg acacggaaat atgcacgaag gatggtgaga caccactgat aaaagccacc
     1141 aagatgagaa atattgaagt ggtggagctg ctgctggata agggggctaa ggtgtctgct
     1201 gtggacaaga aaggggacac tcccctgcac gttgccatcc gagggaggag ccggagactg
     1261 gccgaacttc ttttacggaa ccccaaagat ggacggttac tctacagacc caacaaagca
     1321 ggcgagactc cctacaacat tgactgcagc caccagaaaa gcattttaac tcaaatattt
     1381 ggagccagac acttgtctcc tacggaaaca gatggcgaca tgcttggtta tgatttgtat
     1441 agcagtgccc tggctgatat tctcagtgaa cccaccatgc aaccacccat ttgtgtgggc
     1501 ttgtatgcac agtggggaag tgggaagtct ttcttactca agaaactaga agatgagatg
     1561 aagacgtttg caggacagca gaccgagccc ctcttccagt tctcgtggct catagtgttc
     1621 ctcaccctgc tgctgtgtgg cgggctcggc ctagtgtttg ctttcacggt tgatacaaac
     1681 ctcgccatag ccatttcatt gagtttcctg gctctcatat atatattctt catcgtcatt
     1741 tacttcggtg gacggcggga aggagagagt tggaactggg cctgggctct cagtaccaga
     1801 ctggcaagac acattgggta cctggagctc ctcttcaaac tgatgtttgt gaacccacct
     1861 gaactgccgg agcaaactac aaaggcttta cctgtgaggt ttttgtttac agattacaat
     1921 agactctcca gtgtgggagg agaaacttcc ttggctgaaa tgatcgcgac cctctcagat
     1981 gcttgtgaga gagaattcgg ctttttggct acccgtcttt ttcgagtctt caggactgaa
     2041 gagtctcagg gtaaaaagaa atggaaaaaa acatgctgcc tcccctcttt tgtcatcttt
     2101 ctttttattg ttggctgcat tattgctggg atcacccttc tggctatatt cagagttgac
     2161 ccgaaacatc tgacagtgaa tgctatcctc atttctatcg catctgtcgt ggggttggcc
     2221 ttcgtgctca attgtcgaac atggtggcaa gtgctggact ctctcctgaa ttctcagaga
     2281 aaacgcctcc atagtgctgc ctccaaatta cacaagttaa aaagtgaagg attcatgaaa
     2341 gtcctgaagt gtgaggtgga gctgatggcc aggatggcaa aaaccattga cagcttcact
     2401 caaaaccaga caaggctggt ggttatcatt gacggcctgg atgcctgcga gcaggataaa
     2461 gtccttcaga tgctggacac tgtccgagtt ctgttttcaa agggcccttt tattgccatt
     2521 tttgcaagtg atccgcatat tatcataaag gccatcaacc agaacctgaa tagtgtgctt
     2581 cgtgactcaa atataaacgg acatgactac atgcgcaata tagttcactt accagttttt
     2641 cttaatagtc gtgggcttag caatgcaagg aaattccttg taacttcagc aacaaacggg
     2701 gacattacat gctcagacac cacaggaaca caggaggaca ctgacagaag agtttcacag
     2761 aacagccttg gggagatgac aaagcttggg agcaaaacgg ctctcaacag aagggacact
     2821 taccgcagaa gacagatgca gagaaccatc acgaggcaga tgtcctttga tctcacaaag
     2881 ctgctagtca ctgaggattg gttcagtgac atcagccctc agaccatgag acggctactc
     2941 aatattgttt ctgtgacagg gcggctacta agagccaatc agattacttt caactgggac
     3001 aggctagcca gctggatcaa cctcaccgag cagtggccgt accggacgtc ttggcttata
     3061 ctatatctgg aagagactga aggtctccct gatcagatga ccttaaagac catctatgag
     3121 agaatatcga agaatatccc aacaactaaa gatgttgagc cgcttcttga aattgatgga
     3181 gatataagaa actttgaagt cttcttgtct tcaaggactc cagttctcgt ggctcgagat
     3241 gtaaagacct ttttgccgtg cacagtaaac ctggatccca aacttcggga gatcatcgca
     3301 gatgtccgag ctgcaagaga gcagatcaac ataggaggcc tggcctatcc cccgctccct
     3361 ctgcacgaag gccctcctcg gccaccttct gggtacagtc agcctgcatc tgtctgctct
     3421 tcagcatctt tcaacgggcc gttcccaggc ggggttgtgt ccccacagcc ccacagcagt
     3481 tactacagcg gcttgtccgg accccagcac cccttctaca acaggccatt ctttgcccca
     3541 tacctttaca cgccaaggta ttaccctggc ggttcccaac atctcatctc acgttcatca
     3601 gtaaaaacga gtttgcccag agatcagaac aatggcctac cgtgtgactc tgggtttaac
     3661 aaacagagac aggcaaccgt ccctgccaca ggcagctcac tgttactgag ttcaatgacc
     3721 gtggatgtcg tatgtgagaa actgagacag atagaagggc tggaccagag catgatgcct
     3781 cagtactgca caacaatcaa aaaggcgaac ataaatggcc gggtattgtc tcagtgtaac
     3841 attgatgagc tgaagaaaga gatggctatg aactttggag actggcatct cttcagaagc
     3901 atggtcttag aaatgagaag tgtggaaagc caggtggtcc ctgaagaccc tcgttttctc
     3961 aatgaaaact ccagtgcccc agttcctcat ggagagtctg ctcgccgctc ttctcacact
     4021 gagctgcccc tcacggagct ttccagccag actccataca ccctgaactt cagctttgaa
     4081 gaactgaaca cgcttggcct tgatgaggga gcccctaggc acagtaacct gagttggcag
     4141 tcacaaactc gcagaacccc aagtctctcg agtctcaatt cccaggactc cagtattgaa
     4201 atttcaaagc ttactgataa ggtgcaggcc gagtatagag acgcctatag agagtacatt
     4261 gctcagatgt cccagttaga agggggcaca gggtcgtcca caataagcgg cagatcttct
     4321 ccacatagca catactacat aggccagagc tcttcagggg gctccatcca ttctactcta
     4381 gaacaggaaa gggggaagga gggtgagcta aagcaggagg atgggcggaa gtcattttta
     4441 atgaagaggg gggatgtcat agattactcc tcatcagggg tgtccactaa tgaggcctca
     4501 cccctggacc ccattactga agaagatgag aaatccgacc agtcaggtag taagctactc
     4561 ccaggcaaga aatcctcaga acggcccagt ctcttccaga cagacttgaa gcttaagggg
     4621 ggtggtcttc gctaccagaa actgcccagt gatgaagatg agtcggggac agaagagtca
     4681 gataacaccc cactgctcaa agatgataag gacaaaaagg ctgaaggcaa agcagagaga
     4741 gtgtgcaagt ccccagagca cagtgccgag cccatcagaa cgttcattaa agcaaaagag
     4801 tatttgtccg atgccctcct tgacaaaaag gattcctcag attcgggagt gaggtccaat
     4861 gagagttctc ccaaccactc tctccacaat gaggcagcag acgactccca actggaaaag
     4921 gcaaacctca tagagcttga agatgagggc cacagtggga agcgggggat gccacacagt
     4981 ctgagtggct tgcaagatcc aattatagct cggatgtcca tttgctcgga agacaagaaa
     5041 agcccttccg agtgcagcct gattgctagc agccctgaag aaagctggcc tgcgtgccag
     5101 aaggcctaca atctaaaccg aacgcccagc actgtgactc tgaacaacaa cactgcaccc
     5161 accaacagag ccaatcagaa cttcgatgag atagagggaa tcagggagac gtcccaggtc
     5221 attctgaggc ctggccccag tcccaaccca actgctgtgc agaatgaaaa cctgaagagc
     5281 atggcgcaca agcgaagcca gcgctccagt tacacaaggc tctcgaaaga tgcatctgag
     5341 ctacatgcag cctcctcgga gagcacaggc tttggagaag agagagagag cattctctga
     5401 ggaaggagaa gtgaaaagag tgtgacagac tttgagttga ccattcctca ttactcatca
     5461 tgtttatgca tcacagacac ctgataggtg gtcaggcact ggaagcagac aggaaagcct
     5521 gagagcacag tcaccagtca ttttcattgt caccgtcatc ccaggcactt taaagtctca
     5581 ctgccactat tacacctttc ctggcacaca gctaggcaga gcgatgagag caggctgaag
     5641 acaaaatagg tcaaaagtgt atttaaagtt gaaagggctt aaatcacaag gaaaaaaact
     5701 tagagatctc ctttataaac tattcatgta ccatttgagg gaagatacag tgttagatga
     5761 agcagaaacc aattttattg ttagtgttta atcttggtgc agatttataa gttttagaat
     5821 agcccagaga ctaaaagtga atacttagca aatggatagc cagtgttcta tataggaatc
     5881 attgcttttc agagggctta aagtttaagt agaaaatata tactcaagaa ggcgataaaa
     5941 gctgatgaga aagtgagtta gcagaaccca aagccgtgct gggccgcggt gactcattag
     6001 cagaggagga ggggagggca gtatattcct gggatactct ctccagaccc agcctggctt
     6061 ctgacatcat ccacctgtgc cctcaaaacc gtcttagtct gttctgcaac tcttaagtga
     6121 catacctaac tcagctcatg gctaaggaaa aaaaattaaa gttgtcctgg tgattaaact
     6181 ctggacctcc cacatctaag tcccgagttg acaaactgca tccccagccc aaacatctgt
     6241 tcttccagag atctggaaag acatgggcat aagagtccag taaactctta atgaaggtgt
     6301 cgggaagtgt ttcccttaaa ggggttagtg acagtgacac tagacaagtg acagaattta
     6361 gtttgctgac attaaatcac tgccttctgg accgcaatag tttagtatct gttcctaaat
     6421 cagcatttga gttctgcggg ggttacaaaa tggaggcatg gttttaagaa ggaatttgaa
     6481 agcatccagg gaagcaagaa cagagtcgga gctggtgtga ccatgacact tcccattatt
     6541 gttagcaaag ctaggagaat gaccacatac atttaagtat catgtaggct agagcgtgcc
     6601 tgtcatcatc atgcattgag accgtttgtc ctttgattat agtgtgttta tgtagaccag
     6661 tcctagcagt atgtcctgag ccatgggcag gattgtagat ggcgaggtgg caggtttagt
     6721 gctcgttgag atgtccatct ctgactggac ctgtgtggtt tacttgtagc tgctccgtga
     6781 aacactgcct tgctgtttga gtgtaattga gcattttcat gaagtgtata tgttgcatga
     6841 tctttaacag gtatttttag acattaagaa cagcatgata ttactgacat atgcattagt
     6901 agtatgcagt atcatgtaat gggtacttca gaggcagttt ggtttctttc caggtgagta
     6961 gttatagctc acagtcatgg gaatccttta ttcacactgt ctgtgaagtc gatgctggag
     7021 tgccaaatgc acacggagac taggaacaat aaagacttgc atgccattct gactcatgga
     7081 gttttattca atagagcaat gataattccc attctgatta aaaattaaaa cacgg
//
