LOCUS       NM_057152               3162 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus Kelch-like ECH-associated protein 1 (Keap1),
            mRNA.
ACCESSION   NM_057152
VERSION     NM_057152.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Jiang,G., Shi,L.F., Li,L.J., Duan,X.J. and Zheng,Z.F.
  TITLE     Activation of the p62-Keap1-Nrf2 pathway improves pulmonary
            arterial hypertension in MCT-induced rats by inhibiting autophagy
  JOURNAL   FASEB J 38 (3), e23452 (2024)
   PUBMED   38308640
  REMARK    GeneRIF: Activation of the p62-Keap1-Nrf2 pathway improves
            pulmonary arterial hypertension in MCT-induced rats by inhibiting
            autophagy.
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Geng,W., Yan,S., Li,X., Liu,Q., Zhang,X., Gu,X., Tian,X. and
            Jiang,Y.
  TITLE     miR-432-5p Inhibits the Ferroptosis in Cardiomyocytes Induced by
            Hypoxia/Reoxygenation via Activating Nrf2/SLC7A11 Axis by Degrading
            Keap1
  JOURNAL   Anal Cell Pathol (Amst) 2023, 1293200 (2023)
   PUBMED   37822721
  REMARK    GeneRIF: miR-432-5p Inhibits the Ferroptosis in Cardiomyocytes
            Induced by Hypoxia/Reoxygenation via Activating Nrf2/SLC7A11 Axis
            by Degrading Keap1.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3162)
  AUTHORS   Washimkar,K.R., Tomar,M.S., Ishteyaque,S., Kumar,A., Shrivastava,A.
            and Mugale,M.N.
  TITLE     Benzo[a]pyrene treatment modulates Nrf2/Keap1 axis and changes the
            metabolic profile in rat lung cancer
  JOURNAL   Chem Biol Interact 373, 110373 (2023)
   PUBMED   36736873
  REMARK    GeneRIF: Benzo[a]pyrene treatment modulates Nrf2/Keap1 axis and
            changes the metabolic profile in rat lung cancer.
REFERENCE   4  (bases 1 to 3162)
  AUTHORS   Xu,F., Zhang,J., Zhou,X. and Hao,H.
  TITLE     Lipoxin A4 and its analog attenuate high fat diet-induced
            atherosclerosis via Keap1/Nrf2 pathway
  JOURNAL   Exp Cell Res 412 (1), 113025 (2022)
   PUBMED   35026282
  REMARK    GeneRIF: Lipoxin A4 and its analog attenuate high fat diet-induced
            atherosclerosis via Keap1/Nrf2 pathway.
REFERENCE   5  (bases 1 to 3162)
  AUTHORS   Hu,X., Mu,L., Zhu,L., Chang,X., Nie,L., Wang,L. and Li,G.
  TITLE     Lycium barbarum polysaccharides attenuate cardiovascular oxidative
            stress injury by enhancing the Keap1/Nrf2 signaling pathway in
            exhaustive exercise rats
  JOURNAL   Mol Med Rep 24 (3) (2021)
   PUBMED   34278476
  REMARK    GeneRIF: Lycium barbarum polysaccharides attenuate cardiovascular
            oxidative stress injury by enhancing the Keap1/Nrf2 signaling
            pathway in exhaustive exercise rats.
REFERENCE   6  (bases 1 to 3162)
  AUTHORS   Velichkova,M. and Hasson,T.
  TITLE     Keap1 in adhesion complexes
  JOURNAL   Cell Motil Cytoskeleton 56 (2), 109-119 (2003)
   PUBMED   14506708
REFERENCE   7  (bases 1 to 3162)
  AUTHORS   McMahon,M., Itoh,K., Yamamoto,M. and Hayes,J.D.
  TITLE     Keap1-dependent proteasomal degradation of transcription factor
            Nrf2 contributes to the negative regulation of antioxidant response
            element-driven gene expression
  JOURNAL   J Biol Chem 278 (24), 21592-21600 (2003)
   PUBMED   12682069
  REMARK    GeneRIF: Keap1 negatively regulates Nrf2 by both enhancing its rate
            of proteasomal degradation and altering its subcellular
            distribution
REFERENCE   8  (bases 1 to 3162)
  AUTHORS   Velichkova,M., Guttman,J., Warren,C., Eng,L., Kline,K., Vogl,A.W.
            and Hasson,T.
  TITLE     A human homologue of Drosophila kelch associates with myosin-VIIa
            in specialized adhesion junctions
  JOURNAL   Cell Motil Cytoskeleton 51 (3), 147-164 (2002)
   PUBMED   11921171
REFERENCE   9  (bases 1 to 3162)
  AUTHORS   Dhakshinamoorthy,S. and Jaiswal,A.K.
  TITLE     Functional characterization and role of INrf2 in antioxidant
            response element-mediated expression and antioxidant induction of
            NAD(P)H:quinone oxidoreductase1 gene
  JOURNAL   Oncogene 20 (29), 3906-3917 (2001)
   PUBMED   11439354
REFERENCE   10 (bases 1 to 3162)
  AUTHORS   Chu,C.C. and Paul,W.E.
  TITLE     Expressed genes in interleukin-4 treated B cells identified by cDNA
            representational difference analysis
  JOURNAL   Mol Immunol 35 (8), 487-502 (1998)
   PUBMED   9798653
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Dec 21, 2012 this sequence version replaced NM_057152.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ212383.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760393 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-276               JAXUCZ010000008.1  28053767-28054042   c
            277-962             JAXUCZ010000008.1  28051762-28052447   c
            963-1636            JAXUCZ010000008.1  28048267-28048940   c
            1637-1842           JAXUCZ010000008.1  28046575-28046780   c
            1843-2019           JAXUCZ010000008.1  28046089-28046265   c
            2020-3162           JAXUCZ010000008.1  28044555-28045697   c
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q13"
     gene            1..3162
                     /gene="Keap1"
                     /gene_synonym="Inrf2"
                     /note="Kelch-like ECH-associated protein 1"
                     /db_xref="GeneID:117519"
                     /db_xref="RGD:621619"
     exon            1..276
                     /gene="Keap1"
                     /gene_synonym="Inrf2"
                     /inference="alignment:Splign:2.1.0"
     exon            277..962
                     /gene="Keap1"
                     /gene_synonym="Inrf2"
                     /inference="alignment:Splign:2.1.0"
     CDS             324..2186
                     /gene="Keap1"
                     /gene_synonym="Inrf2"
                     /note="cytosolic inhibitor of Nrf2"
                     /codon_start=1
                     /product="kelch-like ECH-associated protein 1"
                     /protein_id="NP_476493.2"
                     /db_xref="GeneID:117519"
                     /db_xref="RGD:621619"
                     /translation="MQPEPKPSGAPRSSQFLPLWSKCPEGAGDAVMYASTECKAEVTP
                     SQDGNRTFSYTLEDHTKQAFGIMNELRLSQQLCDVTLQVKYEDIPAAQFMAHKVVLAS
                     SSPVFKAMFTNGLREQGMEVVSIEGIHPKVMERLIEFAYTASISVGEKCVLHVMNGAV
                     MYQIDSVVRACSDFLVQQLDPSNAIGIANFAEQIGCTELHQRAREYIYMHFGEEEFFN
                     LSHCQLATLISRDDLNVRCESEVFHACIDWVKYDCPQRRFYVQALLRAVRCHALTPRF
                     LQTQLQKCEILQADARCKDYLVQIFQELTLHKPTQAVPCRAPKVGRLIYTAGGYFRQS
                     LSYLEAYNPSNGSWLRLADLQVPRSGLAGCVVGGLLYAVGGRNNSPDGNTDSSALDCY
                     NPMTNQWSPCASMSVPRNRIGVGVIDGHIYAVGGSHGCIHHSSVERYEPDRDEWHLVA
                     PMLTRRIGVGVAVLNRLLYAVGGFDGTNRLNSAECYYPERNEWRMITPMNTIRSGAGV
                     CVLHSCIYAAGGYDGQDQLNSVERYDVETETWTFVASMKHRRSALGIAVHQGRIYVLG
                     GYDGHTFLDSVECYDPDTDTWSEVTRLTSGRSGVGVAVTMEPCRKQIDQQNCTC"
     exon            963..1636
                     /gene="Keap1"
                     /gene_synonym="Inrf2"
                     /inference="alignment:Splign:2.1.0"
     exon            1637..1842
                     /gene="Keap1"
                     /gene_synonym="Inrf2"
                     /inference="alignment:Splign:2.1.0"
     exon            1843..2019
                     /gene="Keap1"
                     /gene_synonym="Inrf2"
                     /inference="alignment:Splign:2.1.0"
     exon            2020..3162
                     /gene="Keap1"
                     /gene_synonym="Inrf2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aacccggagg caggctccta gggccgccct gacccacgcc ctgctccctc cgcccggcac
       61 ctgcaggaag ggctggaacc gcctctgcgt gcccgccgcc tgtttccgcc ctcccgctcc
      121 tcccacgcgt gccgcccggg accccgcagc accgctgccc cgatccgagg ccccctggct
      181 gccctcggct cctcccggaa gcgcggcgcg tggcggcccg gcggtgcgga ttggacgcgt
      241 ggcaccaaca gagacacccg ggggggtggg acggaggccc ctaatcgcta gtggtggaac
      301 tgtatcttgt catcaggaac cccatgcagc ccgaacccaa gcctagcggg gctccccgca
      361 gcagccagtt cctgcccctg tggtcaaagt gcccggaggg ggcaggggac gcggtgatgt
      421 atgcatccac ggagtgcaag gcggaggtga cgccctcgca ggatggtaac cgaaccttca
      481 gttacacact agaggatcac accaagcagg cttttggcat catgaacgag cttcggctga
      541 gccagcagct ctgtgacgtg accctgcagg tcaaatatga ggacatccca gctgcccaat
      601 tcatggccca caaagtggtg ctggcctcct ccagcccagt ctttaaagct atgttcacca
      661 acgggcttcg ggagcagggc atggaggtgg tgtccattga aggcatccac cccaaggtca
      721 tggagaggct aattgagttc gcctacacgg cctccatctc cgtgggcgag aagtgtgtgc
      781 tgcatgtgat gaacggggca gtcatgtacc agattgacag cgtggtccga gcctgcagcg
      841 acttcctcgt gcagcagctg gaccccagca acgccatcgg catcgccaac ttcgccgagc
      901 agatcggctg cacggaactg caccagcgtg ctcgggagta tatctacatg cacttcgggg
      961 aggaggagtt cttcaacctg tcacactgcc agctggccac gctcatcagc cgcgatgatc
     1021 tgaacgtgcg ctgcgagtcc gaggtgttcc atgcgtgcat cgactgggtc aaatacgact
     1081 gcccgcagcg gcgcttctac gtgcaggcac tgctacgggc cgtgcgctgc cacgctctca
     1141 cgccacgctt cctgcagaca cagctgcaaa agtgcgagat cctgcaggcc gacgcgcgct
     1201 gcaaggacta cctggtgcag atcttccagg agctcacgct gcacaagccc acacaagcgg
     1261 tgccctgccg ggcgcccaaa gtgggccgcc tcatctacac tgcgggcggt tacttccgac
     1321 agtcgctcag ctacctggag gcctacaacc cgagcaatgg ctcctggctg cgcctggcag
     1381 acctacaggt gccccgcagt gggctggcgg gctgcgtggt gggcgggctg ctgtatgctg
     1441 tgggcggccg caacaactcg ccggacggca acactgattc cagcgccctg gactgctaca
     1501 accccatgac caaccagtgg tcgccctgtg cctctatgag cgtgccgcgc aaccgcatcg
     1561 gggtgggggt catagatggc cacatctacg cagtcggggg ttcccacggc tgcatccacc
     1621 acagcagcgt ggagagatat gagccagatc gagacgagtg gcacctagtc gcgccaatgt
     1681 tgacgcggag gatcggcgtg ggcgtggcag tgctcaaccg cttgctgtat gcagtggggg
     1741 gctttgatgg gactaaccgg cttaactcgg cagaatgtta ctacccggag aggaatgagt
     1801 ggcggatgat tacaccaatg aataccatcc ggagcggggc cggggtctgt gtgctgcaca
     1861 gctgcatcta tgccgcagga ggctacgatg ggcaggacca gctgaacagc gtggaacgct
     1921 acgacgtgga gacagagacc tggactttcg tagcctccat gaagcatcgc cggagcgcgc
     1981 tggggattgc tgtgcaccag ggcaggatct acgtcctcgg aggctacgat ggccacactt
     2041 ttctggacag tgtggaatgc tatgacccag acactgatac ctggagtgaa gtgacccgct
     2101 tgacatctgg ccgcagtggg gtgggtgttg ctgtcaccat ggaaccctgt cggaagcaaa
     2161 ttgatcaaca aaactgtacc tgctgaagca cttggactac ctgagcactg gccacaggac
     2221 agaagctgtg catcgctgct gtctctgtac taaagaaaaa ggaagcaaaa ccacaaacaa
     2281 aacacagggc agaagaggca gcagaacaag ccatgccttc ttccacgaag ggcggctggg
     2341 atgccttgta aaggaccttg tggaagacca gaactgaaat ccatgggccc ttctgtcata
     2401 gcccctggag catccaagtc tgcagtctgg gatggggtat gggagggcac cctcgcaggt
     2461 gagaggcccc tgaactccta ccacccacac ccagaccccc tttcaggggg gacaggcaaa
     2521 gcaggaaggt cacatgtgtt ttctttggtt cctgcaactt ggtgatcaat tccaggggac
     2581 aagggaaaga aggaacaggt gaggccaagg ggcagaggct ccctctggag atggggccca
     2641 gggacaaacc tgcagagcag cctctgggct gtaagccctg gacacttatt ttgttaaata
     2701 gccctgtaac tttcccatga gaataaaaaa tggagtaggc agtgccttgc acaccggtcc
     2761 tcagagggca gtggaatccc tcagggagag acagctctgc tattgaaata cacgcagatc
     2821 ctgatcgggg ctggcatctg aaacctgtct gttgtctctg cttacaattg tgcattctac
     2881 tcatctaggg catcctgctc ccttgtggga cacatagttg tctgtctgtt tcggggtatt
     2941 agaagccaat gacccaactt ctgtgacact tctcctgggg cctcctgtct tttgctttga
     3001 gaccagagct catgtagtcc aggatggcct tcaattcaga ccctgaactc ctggccctgc
     3061 tgcctgcccc agcctcctga gttatacctg tataccacca tgcctggttc ctgcttctcc
     3121 tctttgtatc taagcaggaa aataaagatt tctgggacat tg
//
