LOCUS       NP_476493                620 aa            linear   ROD 30-MAR-2024
DEFINITION  kelch-like ECH-associated protein 1 [Rattus norvegicus].
ACCESSION   NP_476493
VERSION     NP_476493.2
DBSOURCE    REFSEQ: accession NM_057152.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 620)
  AUTHORS   Jiang,G., Shi,L.F., Li,L.J., Duan,X.J. and Zheng,Z.F.
  TITLE     Activation of the p62-Keap1-Nrf2 pathway improves pulmonary
            arterial hypertension in MCT-induced rats by inhibiting autophagy
  JOURNAL   FASEB J 38 (3), e23452 (2024)
   PUBMED   38308640
  REMARK    GeneRIF: Activation of the p62-Keap1-Nrf2 pathway improves
            pulmonary arterial hypertension in MCT-induced rats by inhibiting
            autophagy.
REFERENCE   2  (residues 1 to 620)
  AUTHORS   Geng,W., Yan,S., Li,X., Liu,Q., Zhang,X., Gu,X., Tian,X. and
            Jiang,Y.
  TITLE     miR-432-5p Inhibits the Ferroptosis in Cardiomyocytes Induced by
            Hypoxia/Reoxygenation via Activating Nrf2/SLC7A11 Axis by Degrading
            Keap1
  JOURNAL   Anal Cell Pathol (Amst) 2023, 1293200 (2023)
   PUBMED   37822721
  REMARK    GeneRIF: miR-432-5p Inhibits the Ferroptosis in Cardiomyocytes
            Induced by Hypoxia/Reoxygenation via Activating Nrf2/SLC7A11 Axis
            by Degrading Keap1.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 620)
  AUTHORS   Washimkar,K.R., Tomar,M.S., Ishteyaque,S., Kumar,A., Shrivastava,A.
            and Mugale,M.N.
  TITLE     Benzo[a]pyrene treatment modulates Nrf2/Keap1 axis and changes the
            metabolic profile in rat lung cancer
  JOURNAL   Chem Biol Interact 373, 110373 (2023)
   PUBMED   36736873
  REMARK    GeneRIF: Benzo[a]pyrene treatment modulates Nrf2/Keap1 axis and
            changes the metabolic profile in rat lung cancer.
REFERENCE   4  (residues 1 to 620)
  AUTHORS   Xu,F., Zhang,J., Zhou,X. and Hao,H.
  TITLE     Lipoxin A4 and its analog attenuate high fat diet-induced
            atherosclerosis via Keap1/Nrf2 pathway
  JOURNAL   Exp Cell Res 412 (1), 113025 (2022)
   PUBMED   35026282
  REMARK    GeneRIF: Lipoxin A4 and its analog attenuate high fat diet-induced
            atherosclerosis via Keap1/Nrf2 pathway.
REFERENCE   5  (residues 1 to 620)
  AUTHORS   Hu,X., Mu,L., Zhu,L., Chang,X., Nie,L., Wang,L. and Li,G.
  TITLE     Lycium barbarum polysaccharides attenuate cardiovascular oxidative
            stress injury by enhancing the Keap1/Nrf2 signaling pathway in
            exhaustive exercise rats
  JOURNAL   Mol Med Rep 24 (3) (2021)
   PUBMED   34278476
  REMARK    GeneRIF: Lycium barbarum polysaccharides attenuate cardiovascular
            oxidative stress injury by enhancing the Keap1/Nrf2 signaling
            pathway in exhaustive exercise rats.
REFERENCE   6  (residues 1 to 620)
  AUTHORS   Velichkova,M. and Hasson,T.
  TITLE     Keap1 in adhesion complexes
  JOURNAL   Cell Motil Cytoskeleton 56 (2), 109-119 (2003)
   PUBMED   14506708
REFERENCE   7  (residues 1 to 620)
  AUTHORS   McMahon,M., Itoh,K., Yamamoto,M. and Hayes,J.D.
  TITLE     Keap1-dependent proteasomal degradation of transcription factor
            Nrf2 contributes to the negative regulation of antioxidant response
            element-driven gene expression
  JOURNAL   J Biol Chem 278 (24), 21592-21600 (2003)
   PUBMED   12682069
  REMARK    GeneRIF: Keap1 negatively regulates Nrf2 by both enhancing its rate
            of proteasomal degradation and altering its subcellular
            distribution
REFERENCE   8  (residues 1 to 620)
  AUTHORS   Velichkova,M., Guttman,J., Warren,C., Eng,L., Kline,K., Vogl,A.W.
            and Hasson,T.
  TITLE     A human homologue of Drosophila kelch associates with myosin-VIIa
            in specialized adhesion junctions
  JOURNAL   Cell Motil Cytoskeleton 51 (3), 147-164 (2002)
   PUBMED   11921171
REFERENCE   9  (residues 1 to 620)
  AUTHORS   Dhakshinamoorthy,S. and Jaiswal,A.K.
  TITLE     Functional characterization and role of INrf2 in antioxidant
            response element-mediated expression and antioxidant induction of
            NAD(P)H:quinone oxidoreductase1 gene
  JOURNAL   Oncogene 20 (29), 3906-3917 (2001)
   PUBMED   11439354
REFERENCE   10 (residues 1 to 620)
  AUTHORS   Chu,C.C. and Paul,W.E.
  TITLE     Expressed genes in interleukin-4 treated B cells identified by cDNA
            representational difference analysis
  JOURNAL   Mol Immunol 35 (8), 487-502 (1998)
   PUBMED   9798653
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Dec 21, 2012 this sequence version replaced NP_476493.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ212383.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760393 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q13"
     Protein         1..620
                     /product="kelch-like ECH-associated protein 1"
                     /note="cytosolic inhibitor of Nrf2"
                     /calculated_mol_wt=68928
     Region          56..180
                     /region_name="BTB_POZ_KLHL19_KEAP1"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Kelch-like
                     ECH-associated protein 1 (KEAP1); cd18248"
                     /db_xref="CDD:349557"
     Region          95..595
                     /region_name="PHA03098"
                     /note="kelch-like protein; Provisional"
                     /db_xref="CDD:222983"
     Site            order(106,109..110,115..117,125..126,157,161..162)
                     /site_type="other"
                     /note="cullin binding site [polypeptide binding]"
                     /db_xref="CDD:349557"
     Site            order(129,131..132,147..148,150..151,154)
                     /site_type="other"
                     /note="modulator binding site [chemical binding]"
                     /db_xref="CDD:349557"
     Site            151
                     /site_type="other"
                     /note="Sensor for electrophilic agents.
                     /evidence=ECO:0000250|UniProtKB:Q9Z2X8; propagated from
                     UniProtKB/Swiss-Prot (P57790.1)"
     Site            253
                     /site_type="other"
                     /note="Sensor for electrophilic agents.
                     /evidence=ECO:0000250|UniProtKB:Q14145; propagated from
                     UniProtKB/Swiss-Prot (P57790.1)"
     Site            269
                     /site_type="other"
                     /note="Sensor for electrophilic agents.
                     /evidence=ECO:0000250|UniProtKB:Q14145; propagated from
                     UniProtKB/Swiss-Prot (P57790.1)"
     Site            284
                     /site_type="other"
                     /note="Sensor for electrophilic agents.
                     /evidence=ECO:0000250|UniProtKB:Q9Z2X8; propagated from
                     UniProtKB/Swiss-Prot (P57790.1)"
     Region          323..368
                     /region_name="Kelch 1"
                     /note="propagated from UniProtKB/Swiss-Prot (P57790.1)"
     Region          358..405
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          369..419
                     /region_name="Kelch 2"
                     /note="propagated from UniProtKB/Swiss-Prot (P57790.1)"
     Region          409..452
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          420..466
                     /region_name="Kelch 3"
                     /note="propagated from UniProtKB/Swiss-Prot (P57790.1)"
     Site            430
                     /site_type="other"
                     /note="Sensor for electrophilic agents.
                     /evidence=ECO:0000250|UniProtKB:Q9Z2X8; propagated from
                     UniProtKB/Swiss-Prot (P57790.1)"
     Region          456..499
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          467..513
                     /region_name="Kelch 4"
                     /note="propagated from UniProtKB/Swiss-Prot (P57790.1)"
     Region          503..547
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          515..560
                     /region_name="Kelch 5"
                     /note="propagated from UniProtKB/Swiss-Prot (P57790.1)"
     Region          550..593
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          561..607
                     /region_name="Kelch 6"
                     /note="propagated from UniProtKB/Swiss-Prot (P57790.1)"
     CDS             1..620
                     /gene="Keap1"
                     /gene_synonym="Inrf2"
                     /coded_by="NM_057152.2:324..2186"
                     /db_xref="GeneID:117519"
                     /db_xref="RGD:621619"
ORIGIN      
        1 mqpepkpsga prssqflplw skcpegagda vmyastecka evtpsqdgnr tfsytledht
       61 kqafgimnel rlsqqlcdvt lqvkyedipa aqfmahkvvl assspvfkam ftnglreqgm
      121 evvsiegihp kvmerliefa ytasisvgek cvlhvmngav myqidsvvra csdflvqqld
      181 psnaigianf aeqigctelh qrareyiymh fgeeeffnls hcqlatlisr ddlnvrcese
      241 vfhacidwvk ydcpqrrfyv qallravrch altprflqtq lqkceilqad arckdylvqi
      301 fqeltlhkpt qavpcrapkv grliytaggy frqslsylea ynpsngswlr ladlqvprsg
      361 lagcvvggll yavggrnnsp dgntdssald cynpmtnqws pcasmsvprn rigvgvidgh
      421 iyavggshgc ihhssverye pdrdewhlva pmltrrigvg vavlnrllya vggfdgtnrl
      481 nsaecyyper newrmitpmn tirsgagvcv lhsciyaagg ydgqdqlnsv erydvetetw
      541 tfvasmkhrr salgiavhqg riyvlggydg htfldsvecy dpdtdtwsev trltsgrsgv
      601 gvavtmepcr kqidqqnctc
//
