LOCUS       NM_198762               3845 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus potassium sodium-activated channel subfamily T
            member 2 (Kcnt2), mRNA.
ACCESSION   NM_198762 XM_006249942 XM_222699
VERSION     NM_198762.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Gururaj,S., Palmer,E.E., Sheehan,G.D., Kandula,T., Macintosh,R.,
            Ying,K., Morris,P., Tao,J., Dias,K.R., Zhu,Y., Dinger,M.E.,
            Cowley,M.J., Kirk,E.P., Roscioli,T., Sachdev,R., Duffey,M.E.,
            Bye,A. and Bhattacharjee,A.
  TITLE     A De Novo Mutation in the Sodium-Activated Potassium Channel KCNT2
            Alters Ion Selectivity and Causes Epileptic Encephalopathy
  JOURNAL   Cell Rep 21 (4), 926-933 (2017)
   PUBMED   29069600
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Budelli,G., Sun,Q., Ferreira,J., Butler,A., Santi,C.M. and
            Salkoff,L.
  TITLE     SLO2 Channels Are Inhibited by All Divalent Cations That Activate
            SLO1 K+ Channels
  JOURNAL   J Biol Chem 291 (14), 7347-7356 (2016)
   PUBMED   26823461
  REMARK    GeneRIF: SLO2 Channels Are Inhibited by All Divalent Cations That
            Activate SLO1 K+ Channels
REFERENCE   3  (bases 1 to 3845)
  AUTHORS   Tomasello,D.L., Gancarz-Kausch,A.M., Dietz,D.M. and
            Bhattacharjee,A.
  TITLE     Transcriptional Regulation of the Sodium-activated Potassium
            Channel SLICK (KCNT2) Promoter by Nuclear Factor-kappaB
  JOURNAL   J Biol Chem 290 (30), 18575-18583 (2015)
   PUBMED   26100633
  REMARK    GeneRIF: Our data show SLICK expression is predominantly under the
            control of NFkappaB. Because neuronal NFkappaB activation occurs
            during stressful stimuli such as hypoxia and injury, our findings
            suggest that SLICK is a neuroprotective gene.
REFERENCE   4  (bases 1 to 3845)
  AUTHORS   Tejada,M.A., Stople,K., Hammami Bomholtz,S., Meinild,A.K.,
            Poulsen,A.N. and Klaerke,D.A.
  TITLE     Cell volume changes regulate slick (Slo2.1), but not slack (Slo2.2)
            K+ channels
  JOURNAL   PLoS One 9 (10), e110833 (2014)
   PUBMED   25347289
  REMARK    GeneRIF: Slick channels, in contrast to the similar Slack channels,
            are the only high-conductance K+ channels strongly sensitive to
            small changes in cell volume.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3845)
  AUTHORS   Chen,H., Kronengold,J., Yan,Y., Gazula,V.R., Brown,M.R., Ma,L.,
            Ferreira,G., Yang,Y., Bhattacharjee,A., Sigworth,F.J., Salkoff,L.
            and Kaczmarek,L.K.
  TITLE     The N-terminal domain of Slack determines the formation and
            trafficking of Slick/Slack heteromeric sodium-activated potassium
            channels
  JOURNAL   J Neurosci 29 (17), 5654-5665 (2009)
   PUBMED   19403831
  REMARK    GeneRIF: Heteromer formation between Slick and Slack-B requires a
            specific epitope that is found in the N-terminal domain of Slack-B
            and that is absent in the Slack-A isoform.
REFERENCE   6  (bases 1 to 3845)
  AUTHORS   Gao,S.B., Wu,Y., Lu,C.X., Guo,Z.H., Li,C.H. and Ding,J.P.
  TITLE     Slack and Slick KNa channels are required for the depolarizing
            afterpotential of acutely isolated, medium diameter rat dorsal root
            ganglion neurons
  JOURNAL   Acta Pharmacol Sin 29 (8), 899-905 (2008)
   PUBMED   18664322
  REMARK    GeneRIF: We conclude that Slack and Slick K(Na) channels are
            required for depolarizing afterpotential of medium diameter rat
            dorsal root ganglion neurons
REFERENCE   7  (bases 1 to 3845)
  AUTHORS   Bhattacharjee,A., von Hehn,C.A., Mei,X. and Kaczmarek,L.K.
  TITLE     Localization of the Na+-activated K+ channel Slick in the rat
            central nervous system
  JOURNAL   J Comp Neurol 484 (1), 80-92 (2005)
   PUBMED   15717307
  REMARK    GeneRIF: we report the localization of Slick in the rat central
            nervous system using in situ and immunohistochemical techniques
REFERENCE   8  (bases 1 to 3845)
  AUTHORS   Zhang,Y., Joiner,W.J., Bhattacharjee,A., Rassendren,F.,
            Magoski,N.S. and Kaczmarek,L.K.
  TITLE     The appearance of a protein kinase A-regulated splice isoform of
            slo is associated with the maturation of neurons that control
            reproductive behavior
  JOURNAL   J Biol Chem 279 (50), 52324-52330 (2004)
   PUBMED   15375169
REFERENCE   9  (bases 1 to 3845)
  AUTHORS   Bhattacharjee,A., Joiner,W.J., Wu,M., Yang,Y., Sigworth,F.J. and
            Kaczmarek,L.K.
  TITLE     Slick (Slo2.1), a rapidly-gating sodium-activated potassium channel
            inhibited by ATP
  JOURNAL   J Neurosci 23 (37), 11681-11691 (2003)
   PUBMED   14684870
  REMARK    GeneRIF: Cloning and regulation of slick channel activity.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000013.1.
            
            On or before Dec 3, 2020 this sequence version replaced
            XM_006249942.3, NM_198762.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760393,
                              SAMN12840105 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-207               JAXUCZ010000013.1  54215307-54215513
            208-287             JAXUCZ010000013.1  54310394-54310473
            288-387             JAXUCZ010000013.1  54313068-54313167
            388-436             JAXUCZ010000013.1  54325230-54325278
            437-496             JAXUCZ010000013.1  54327845-54327904
            497-571             JAXUCZ010000013.1  54340915-54340989
            572-655             JAXUCZ010000013.1  54342079-54342162
            656-750             JAXUCZ010000013.1  54350811-54350905
            751-931             JAXUCZ010000013.1  54393953-54394133
            932-1096            JAXUCZ010000013.1  54395209-54395373
            1097-1233           JAXUCZ010000013.1  54398073-54398209
            1234-1297           JAXUCZ010000013.1  54400666-54400729
            1298-1406           JAXUCZ010000013.1  54429171-54429279
            1407-1515           JAXUCZ010000013.1  54464994-54465102
            1516-1665           JAXUCZ010000013.1  54488349-54488498
            1666-1895           JAXUCZ010000013.1  54489872-54490101
            1896-2109           JAXUCZ010000013.1  54493982-54494195
            2110-2215           JAXUCZ010000013.1  54496168-54496273
            2216-2388           JAXUCZ010000013.1  54503658-54503830
            2389-2460           JAXUCZ010000013.1  54511325-54511396
            2461-2595           JAXUCZ010000013.1  54514330-54514464
            2596-2707           JAXUCZ010000013.1  54532356-54532467
            2708-2809           JAXUCZ010000013.1  54543194-54543295
            2810-2893           JAXUCZ010000013.1  54554273-54554356
            2894-3022           JAXUCZ010000013.1  54555391-54555519
            3023-3323           JAXUCZ010000013.1  54585848-54586148
            3324-3408           JAXUCZ010000013.1  54596847-54596931
            3409-3845           JAXUCZ010000013.1  54607488-54607924
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q21"
     gene            1..3845
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /note="potassium sodium-activated channel subfamily T
                     member 2"
                     /db_xref="GeneID:304827"
                     /db_xref="RGD:735074"
     exon            1..207
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..3520
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /note="sequence like an intermediate conductance potassium
                     channel subunit; sodium and chloride-activated
                     ATP-sensitive potassium channel Slo2.1; sodium- and
                     chloride-activated ATP-sensitive potassium channel;
                     potassium channel, subfamily T, member 2; potassium
                     channel, sodium-activated subfamily T, member 2"
                     /codon_start=1
                     /product="potassium channel subfamily T member 2"
                     /protein_id="NP_942057.2"
                     /db_xref="GeneID:304827"
                     /db_xref="RGD:735074"
                     /translation="MVDLESEVPPLPPRYRFRDLLLGDQGWQNDDRVQVEFYMNENTF
                     KERLKLFFIKNQRSSLRIRLFNFSLKLLSCLLYIIRVLLEKPSQGNDWSHIFWVNRSL
                     PLWGLQVSVALISLFETILLGYLSYKGNIWEQILRVPFILEIINAVPFIISIFWPTLR
                     NLFVPVFLNCWLAKHALENMINDLHRAIQRTQSAMFNQVLILISTLLCLIFTCICGIQ
                     HLERIGKKLNLFDSLYFCIVTFSTVGFGDVTPETWSSKLFVVAMICVALVVLPIQFEQ
                     LAYLWMERQKSGGNYSRHRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVI
                     LCPTEMDVQVRRVLQIPMWSQRVIYLQGSALKDQDLLRAKMDNAEACFILSSRCEVDR
                     TSSDHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCI
                     CPATSTLITLLVHTSRGQEGQQSPEQWQKTYGRCSGNEVYHIVLEESTFFAEYEGKSF
                     TYASFHAHKKFGVCLVGVRREDNKNILLNPGPRYIMNASDICFYINITKEENSAFKNQ
                     DQQRKSNVSRSFYHGPSRLPVHSIIASMGTVAIDLQDTSCRAASGPTLALPSEGGKEL
                     RRPSIAPVLEVADTSSIQTCDLLSDQSEDETTPDEETSSNLEYAKGYPPYSPYIGSSP
                     TFCHLLQEKVPFCCLRLDKSCQHNYYEDAKAYGFKNKLIIVAAETAGNGLYNFIVPLR
                     AYYRPKKELNPIVLLLDNPPDMHFLDAICWFPMVYYMVGSIDNLDDLLRCGVTFAANM
                     VVVDKESTMSAEEDYMADAKTIVNVQTLFRLFSSLSIITELTHPANMRFMQFRAKDCY
                     SLALSKLEKKERERGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMISITRLLL
                     GLDTIPGSGFLCSMKITEDDLWIRTYARLYQKLCSSTGDVPIGIYRTESQKLTTSESQ
                     ISISVEEWEDTKDVKDPGHHRSIHRNSTSSDQSDHPLLRRKSMQWARRLSRKGPKHSG
                     KTAEKITQQRLNLYRRSERQELAELVKNRMKHLGLSTVGYDEMNDHQSTLSYILINPS
                     PDTRLELNDVVYLIRPDPLSYLPNSEPSRKNSICNAAVQDSREETQL"
     exon            208..287
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            288..387
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            388..436
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            437..496
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            497..571
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            572..655
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            656..750
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            751..931
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1096
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1233
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1297
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1406
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1515
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1665
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1895
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..2109
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            2110..2215
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            2216..2388
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            2389..2460
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            2461..2595
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            2596..2707
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            2708..2809
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            2810..2893
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            2894..3022
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            3023..3323
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            3324..3408
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
     exon            3409..3845
                     /gene="Kcnt2"
                     /gene_synonym="Slick"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cccccggccc ttctcccacc ctccccagca cggcaacccc ctccgcaggg cttgctggcc
       61 ctttctgccc tcctccatcc cttgttggat gttgcccatt ccttaagaaa ggatggttga
      121 tttggagagc gaagtacccc ctctgcctcc caggtacaga ttccgtgatt tgctgctagg
      181 ggaccaaggc tggcagaatg atgacagggt tcaagtcgaa ttctacatga atgaaaacac
      241 atttaaagag agattaaagt tgtttttcat aaaaaaccag cgatcaagtc taagaatccg
      301 cttgttcaac ttttccctca aattgttaag ctgtttgtta tacattatcc gtgtgttgct
      361 tgagaaacct tcacagggaa atgattggtc tcacatattt tgggttaaca gaagcctgcc
      421 tttatggggt ttacaggtct cggtggcatt gataagtcta tttgaaacaa tactccttgg
      481 atatctcagt tataagggga atatctggga gcagatttta cgagttccat tcatcttgga
      541 aataatcaat gcagttccct tcatcatctc aatattctgg cctacgttaa gaaatctctt
      601 tgttccagtt tttctaaatt gttggcttgc caaacatgcc ttggaaaata tgattaatga
      661 tcttcatcga gccattcagc gtacacagtc tgcaatgttt aatcaagttc tgattttgat
      721 atctacttta ctatgcctta tcttcacttg catttgtgga attcagcatc tggaacgaat
      781 tggaaagaaa ctcaatctct ttgactccct ttatttctgc attgtgacat tttctactgt
      841 gggctttggg gatgttactc ctgaaacatg gtcctccaag ctttttgttg ttgctatgat
      901 ctgcgttgct cttgtagttc tccccataca gtttgaacag ctggcctatt tgtggatgga
      961 gagacaaaag tcgggaggaa actatagtcg acacagagct cagactgaga agcatgttgt
     1021 tctgtgtgtc agctcattga agattgactt gcttatggat tttttgaatg aattctatgc
     1081 acatccaaga ctgcaggatt attatgtggt gattttgtgt cccacggaga tggatgtgca
     1141 agttcggaga gtgctacaga ttccaatgtg gtcccagaga gtgatctacc ttcaaggctc
     1201 tgcccttaaa gaccaggatc tcctgagagc aaagatggac aatgctgagg cctgtttcat
     1261 tttgagtagc cgctgtgaag tcgatagaac atcatctgat caccagacaa ttttgagagc
     1321 atgggctgta aaagattttg ccccaaattg tcctctgtat gttcagatat taaaaccaga
     1381 aaataaattc cacatcaaat ttgcagatca tgttgtttgt gaagaagagt ttaagtacgc
     1441 catgttagct ttaaactgta tatgcccagc aacatctaca cttattacac tactggttca
     1501 tacctctaga gggcaagaag ggcagcagtc acctgaacag tggcagaaga cgtatgggag
     1561 gtgctcagga aatgaagtct accacatcgt tctggaagaa agtacatttt ttgctgaata
     1621 tgaagggaag agttttacct atgcttcttt ccatgcacac aaaaagtttg gtgtctgctt
     1681 ggttggtgtt aggagggagg ataataaaaa cattttgctg aatccaggtc ctcgatacat
     1741 tatgaatgct tcagacatat gtttttatat taatattacc aaagaagaaa attcagcatt
     1801 taagaatcaa gaccaacaga gaaaaagtaa tgtgtcaagg tcattttatc atgggccttc
     1861 cagattgcct gtccacagta tcattgccag catgggtact gtggctatag acttacaaga
     1921 cacaagctgt agagcagcaa gtggccctac actggctctt ccttcagagg gaggcaagga
     1981 actaagaaga cctagcattg ctcctgtttt agaggtagca gatacatcat caattcaaac
     2041 ctgcgatctt ctaagtgacc agtcagaaga tgaaactaca ccagatgaag aaacatcttc
     2101 aaatttagag tatgccaaag gctacccacc ttattctcca tacataggaa gttcacctac
     2161 tttttgtcat ctacttcaag aaaaagtgcc cttttgctgc ttaagattag acaagagttg
     2221 ccagcataac tactatgagg atgcaaaagc ctatggattc aaaaataaac taattatagt
     2281 tgcagctgaa acggctggga acggattata taattttatt gtacctctca gggcatatta
     2341 tagaccaaag aaagaattaa atcccatagt tcttctattg gataatccgc cagatatgca
     2401 ttttctggat gcaatctgtt ggtttccaat ggtttactac atggtgggct ctattgacaa
     2461 cctagatgat ttgctcaggt gtggagtgac cttcgctgcc aacatggtgg ttgtggacaa
     2521 agaaagcacc atgagtgcag aggaagacta catggcagat gccaagacga ttgtgaatgt
     2581 gcagactctt ttcaggttgt tttcgagtct cagtattatc actgagctta ctcatccagc
     2641 aaatatgaga ttcatgcaat tcagagccaa ggactgttac tctcttgcac tttcaaaact
     2701 ggaaaagaaa gagcgagaac gaggttctaa cctggccttc atgttccgac tgcctttcgc
     2761 tgcggggaga gtgttcagca tcagtatgct ggacacgctg ctttatcagt catttgtgaa
     2821 agattatatg atttctatca ccagacttct tttgggactg gacaccatac caggatcggg
     2881 gtttctttgt tctatgaaaa ttactgaaga tgacttgtgg atcagaacgt atgccagact
     2941 ctatcagaag ctgtgttctt ctactggaga tgttcccatt gggatctaca gaacagaatc
     3001 tcagaagcta actacatctg agtctcaaat atccatcagt gtggaagagt gggaagacac
     3061 caaagatgtc aaagacccag ggcaccaccg cagcattcac cgcaactcaa cctctagcga
     3121 ccagtcagac caccccttgc tgcggaggaa gagcatgcag tgggcccgga ggctgagcag
     3181 aaaagggcca aagcactctg gtaaaactgc agaaaagata acccagcaac gactgaatct
     3241 ctacaggagg tcggaaagac aggagcttgc tgaacttgtg aaaaacagaa tgaaacacct
     3301 gggcctctct acggtgggct acgatgaaat gaatgatcat cagagcaccc tttcctacat
     3361 cctgattaat ccatctccag acaccaggct tgagctgaat gatgttgtat atttgatccg
     3421 accagatcca ctgtcctacc ttccgaacag tgaacccagt aggaaaaaca gcatctgcaa
     3481 tgccgctgtt caagattctc gggaggaaac acaactctga tgaaaagaaa ataagagaca
     3541 ttatattcct tgcagggatc ttgcttaaaa tcattaaaat gttgcagatt gtctaaagaa
     3601 gctgactgga atgtattcaa tttctcatgt tgcttctata taaaacaaaa tctattctct
     3661 ttgaagtaca gatcattttg aaacataatg tgatatttgt tgacattttc ctgcttaata
     3721 tttcaacttc attggaggag attctcaaaa taactataaa atgcagaaat gaaaaatctg
     3781 atatttagtt gtttaagaac taagagtgta taaaaatttt aaaatccacc tatattaagt
     3841 acatc
//
