LOCUS       NP_068625               1237 aa            linear   ROD 08-FEB-2025
DEFINITION  potassium channel subfamily T member 1 isoform 1 [Rattus
            norvegicus].
ACCESSION   NP_068625
VERSION     NP_068625.2
DBSOURCE    REFSEQ: accession NM_021853.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 1237)
  AUTHORS   Wu,J., El-Hassar,L., Datta,D., Thomas,M., Zhang,Y., Jenkins,D.P.,
            DeLuca,N.J., Chatterjee,M., Gribkoff,V.K., Arnsten,A.F.T. and
            Kaczmarek,L.K.
  TITLE     Interaction Between HCN and Slack Channels Regulates mPFC Pyramidal
            Cell Excitability in Working Memory Circuits
  JOURNAL   Mol Neurobiol 61 (4), 2430-2445 (2024)
   PUBMED   37889366
  REMARK    GeneRIF: Interaction Between HCN and Slack Channels Regulates mPFC
            Pyramidal Cell Excitability in Working Memory Circuits.
REFERENCE   2  (residues 1 to 1237)
  AUTHORS   Xu,J., Lv,Y.T., Zhao,X.Y., Wang,J.J., Shen,Z.S., Li,J., Zhang,F.F.,
            Liu,J., Wang,X.H., Xu,Y., Geng,Q., Ding,Y.T., Xu,J.J., Tan,M.J.,
            Li,Z.X., Wang,R., Chen,J., Sun,W., Cui,M., Logothetis,D.E.,
            Cao,J.L., Tang,Q.Y. and Zhang,Z.
  TITLE     Identification of Sodium- and Chloride-Sensitive Sites in the Slack
            Channel
  JOURNAL   J Neurosci 43 (15), 2665-2681 (2023)
   PUBMED   36898835
  REMARK    GeneRIF: Identification of Sodium- and Chloride-Sensitive Sites in
            the Slack Channel.
REFERENCE   3  (residues 1 to 1237)
  AUTHORS   Pryce,K.D., Powell,R., Agwa,D., Evely,K.M., Sheehan,G.D., Nip,A.,
            Tomasello,D.L., Gururaj,S. and Bhattacharjee,A.
  TITLE     Magi-1 scaffolds NaV1.8 and Slack KNa channels in dorsal root
            ganglion neurons regulating excitability and pain
  JOURNAL   FASEB J 33 (6), 7315-7330 (2019)
   PUBMED   30860870
  REMARK    GeneRIF: Magi-1 bound to sequence like a calcium-activated
            potassium channel sodium-activated (Slack) potassium channels,
            demonstrating macrocomplexing with NaV1.8 channels.
REFERENCE   4  (residues 1 to 1237)
  AUTHORS   Evely,K.M., Pryce,K.D. and Bhattacharjee,A.
  TITLE     The Phe932Ile mutation in KCNT1 channels associated with severe
            epilepsy, delayed myelination and leukoencephalopathy produces a
            loss-of-function channel phenotype
  JOURNAL   Neuroscience 351, 65-70 (2017)
   PUBMED   28366665
  REMARK    GeneRIF: characterized an analogous mutation in the rat Slack
            channel and unexpectedly found this mutation to produce a
            loss-of-function phenotype
REFERENCE   5  (residues 1 to 1237)
  AUTHORS   Tejada,M.A., Hashem,N., Calloe,K. and Klaerke,D.A.
  TITLE     Heteromeric Slick/Slack K+ channels show graded sensitivity to cell
            volume changes
  JOURNAL   PLoS One 12 (2), e0169914 (2017)
   PUBMED   28222129
  REMARK    GeneRIF: mRNA of aquaporin 1 was co-expressed in Xenopus oocytes
            with homomeric or heteromeric Slick and Slack alpha-subunits.
            Hypotonic or hypertonic buffers caused changes in current. The
            heteromeric channel had a characteristic graded sensitivity to
            small and fast changes in cell volume. This the cell volume
            sensitivity is dependent on the number of volume sensitive Slick
            alpha-subunits in the tetrameric channels.
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 1237)
  AUTHORS   Ruffin,V.A., Gu,X.Q., Zhou,D., Douglas,R.M., Sun,X., Trouth,C.O.
            and Haddad,G.G.
  TITLE     The sodium-activated potassium channel Slack is modulated by
            hypercapnia and acidosis
  JOURNAL   Neuroscience 151 (2), 410-418 (2008)
   PUBMED   18082331
  REMARK    GeneRIF: Slack may play an important role in pathological
            conditions during which there is an increase in the intracellular
            concentrations of both acid and Na(+), such as in ischemia/hypoxia
REFERENCE   7  (residues 1 to 1237)
  AUTHORS   Bhattacharjee,A., Joiner,W.J., Wu,M., Yang,Y., Sigworth,F.J. and
            Kaczmarek,L.K.
  TITLE     Slick (Slo2.1), a rapidly-gating sodium-activated potassium channel
            inhibited by ATP
  JOURNAL   J Neurosci 23 (37), 11681-11691 (2003)
   PUBMED   14684870
REFERENCE   8  (residues 1 to 1237)
  AUTHORS   Yuan,A., Santi,C.M., Wei,A., Wang,Z.W., Pollak,K., Nonet,M.,
            Kaczmarek,L., Crowder,C.M. and Salkoff,L.
  TITLE     The sodium-activated potassium channel is encoded by a member of
            the Slo gene family
  JOURNAL   Neuron 37 (5), 765-773 (2003)
   PUBMED   12628167
  REMARK    GeneRIF: The Slo2 gene (also called Slack), is heterologously
            expressed, shares many properties of native K(Na) channels
            including activation by intracellular Na+, high conductance, and
            prominent subconductance states.
REFERENCE   9  (residues 1 to 1237)
  AUTHORS   Bhattacharjee,A., Gan,L. and Kaczmarek,L.K.
  TITLE     Localization of the Slack potassium channel in the rat central
            nervous system
  JOURNAL   J Comp Neurol 454 (3), 241-254 (2002)
   PUBMED   12442315
  REMARK    GeneRIF: Localization of Slack protein has been determined to be
            most prominent in the brainstem, in particular the trigeminal
            system and reticular formation, in both cell bodies and axonal
            fibers of associated nuclei.
REFERENCE   10 (residues 1 to 1237)
  AUTHORS   Joiner,W.J., Tang,M.D., Wang,L.Y., Dworetzky,S.I., Boissard,C.G.,
            Gan,L., Gribkoff,V.K. and Kaczmarek,L.K.
  TITLE     Formation of intermediate-conductance calcium-activated potassium
            channels by interaction of Slack and Slo subunits
  JOURNAL   Nat Neurosci 1 (6), 462-469 (1998)
   PUBMED   10196543
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Oct 27, 2022 this sequence version replaced NP_068625.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF089730.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3p13"
     Protein         1..1237
                     /product="potassium channel subfamily T member 1 isoform
                     1"
                     /function="heteromultimerizes with maxi-K (Slo) subunits
                     to generate intermediate-conductance calcium-activated
                     potassium channels"
                     /note="sequence like a calcium-activated potassium channel
                     subunit; potassium channel subunit (Slack); potassium
                     channel, subfamily T, member 1; potassium channel,
                     sodium-activated subfamily T, member 1"
                     /calculated_mol_wt=139499
     Region          1..45
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z258.2)"
     Site            94..126
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z258.2)"
     Site            133
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9Z258.2)"
     Site            137
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9Z258.2)"
     Region          143..323
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Site            154..178
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z258.2)"
     Site            193..208
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z258.2)"
     Site            216..233
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z258.2)"
     Site            247..274
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z258.2)"
     Region          258..331
                     /region_name="Ion_trans_2"
                     /note="Ion channel; pfam07885"
                     /db_xref="CDD:462301"
     Site            305..338
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z258.2)"
     Region          485..579
                     /region_name="BK_channel_a"
                     /note="Calcium-activated BK potassium channel alpha
                     subunit; pfam03493"
                     /db_xref="CDD:460947"
     Region          656..685
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z258.2)"
     Region          1049..1077
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z258.2)"
     Region          1208..1235
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z258.2)"
     CDS             1..1237
                     /gene="Kcnt1"
                     /gene_synonym="rSlo2; Slack"
                     /coded_by="NM_021853.2:25..3738"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="GeneID:60444"
                     /db_xref="RGD:621106"
ORIGIN      
        1 maraklprsp segkagpgdt pagsaapeep hglspllptr gggsvgsdvg qrlhvedfsl
       61 dsslsqvqve fyvnentfke rlklffiknq rsslrirlfn fslklltcll yivrvlldnp
      121 dqgigcwgct kynytfngss sefhwapilw verkmalwvi qvivatisfl etmlliylsy
      181 kgniweqifh vsfvlemint lpfiitvfwp plrnlfipvf lncwlakhal enmindfhra
      241 ilrtqsamfn qvlilfctll clvftgtcgi qhleraggnl nlltsfyfci vtfstvgfgd
      301 vtpkiwpsql lvvilicvtl vvlplqfeel vylwmerqks ggnysrhrar tekhvvlcvs
      361 slkidllmdf lnefyahprl qdyyvvilcp semdvqvrrv lqiplwsqrv iylqgsalkd
      421 qdlmrakmdn geacfilssr nevdrtaadh qtilrawavk dfapncplyv qilkpenkfh
      481 vkfadhvvce eeckyamlal ncicpatstl itllvhtsrg qegqespeqw qrmygrcsgn
      541 evyhirmgds kffreyegks ftyaafhahk kygvcliglk reenksilln pgprhilaas
      601 dtcfyinitk eensafifkq eekqkrrgla gqalyegpsr lpvhsiiasm vamdlqntdc
      661 rpsqggsggg ggkltlpten gsgsrrpsia pvleladssa llpcdllsdq sedevtpsdd
      721 eglsvveyvk gyppnspyig ssptlchllp vkapfcclrl dkgckhnsye dakaygfknk
      781 liivsaetag nglynfivpl rayyrsrrel npivllldnk pdhhfleaic cfpmvyymeg
      841 svdnldsllq cgiiyadnlv vvdkestmsa eedymadakt ivnvqtmfrl fpslsittel
      901 thpsnmrfmq frakdsysla lsklekqere ngsnlafmfr lpfaagrvfs ismldtllyq
      961 sfvkdymiti trlllgldtt pgsgylcamk vteddlwirt ygrlfqklcs ssaeipigiy
     1021 rtechvfsse phdlraqsqi svnmedcedt reakgpwgtr aasgggsthg rhggsadpve
     1081 hpllrrkslq warklsrkss kqagkapmtt dwitqqrlsl yrrserqels elvknrmkhl
     1141 glpttgyedv anltasdvmn rvnlgylqde mndhhqntls yvlinpppdt rlepndivyl
     1201 irsdplahvt sssqsrkssc snklsscnpe trdetql
//
