LOCUS       NM_019314               2060 bp    mRNA    linear   ROD 17-DEC-2024
DEFINITION  Rattus norvegicus potassium calcium-activated channel subfamily N
            member 2 (Kcnn2), transcript variant 2, mRNA.
ACCESSION   NM_019314
VERSION     NM_019314.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Rahman,M.A., Orfali,R., Dave,N., Lam,E., Naguib,N., Nam,Y.W. and
            Zhang,M.
  TITLE     KCa 2.2 (KCNN2): A physiologically and therapeutically important
            potassium channel
  JOURNAL   J Neurosci Res 101 (11), 1699-1710 (2023)
   PUBMED   37466411
  REMARK    GeneRIF: KCa 2.2 (KCNN2): A physiologically and therapeutically
            important potassium channel.
            Review article
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Nam,Y.W., Rahman,M.A., Yang,G., Orfali,R., Cui,M. and Zhang,M.
  TITLE     Loss-of-function KCa2.2 mutations abolish channel activity
  JOURNAL   Am J Physiol Cell Physiol 324 (3), C658-C664 (2023)
   PUBMED   36717104
  REMARK    GeneRIF: Loss-of-function KCa2.2 mutations abolish channel
            activity.
REFERENCE   3  (bases 1 to 2060)
  AUTHORS   Yang,B., Jiang,Q., He,S., Li,T., Ou,X., Chen,T., Fan,X., Jiang,F.,
            Zeng,X., Huang,C.L., Lei,M. and Tan,X.
  TITLE     Ventricular SK2 upregulation following angiotensin II challenge:
            Modulation by p21-activated kinase-1
  JOURNAL   J Mol Cell Cardiol 164, 110-125 (2022)
   PUBMED   34774547
  REMARK    GeneRIF: Ventricular SK2 upregulation following angiotensin II
            challenge: Modulation by p21-activated kinase-1.
REFERENCE   4  (bases 1 to 2060)
  AUTHORS   Autuori,E., Sedlak,P., Xu,L., C Ridder,M., Tedoldi,A. and Sah,P.
  TITLE     rSK1 in Rat Neurons: A Controller of Membrane rSK2?
  JOURNAL   Front Neural Circuits 13, 21 (2019)
   PUBMED   31001092
  REMARK    GeneRIF: rSK1 appears to act as chaperone for rSK2 channels and
            expression of rSK1 may control the level of functional SK current
            in rat neurons.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2060)
  AUTHORS   Ewin,S.E., Morgan,J.W., Niere,F., McMullen,N.P., Barth,S.H.,
            Almonte,A.G., Raab-Graham,K.F. and Weiner,J.L.
  TITLE     Chronic Intermittent Ethanol Exposure Selectively Increases
            Synaptic Excitability in the Ventral Domain of the Rat Hippocampus
  JOURNAL   Neuroscience 398, 144-157 (2019)
   PUBMED   30481568
REFERENCE   6  (bases 1 to 2060)
  AUTHORS   Schumacher,M.A., Crum,M. and Miller,M.C.
  TITLE     Crystal structures of apocalmodulin and an apocalmodulin/SK
            potassium channel gating domain complex
  JOURNAL   Structure 12 (5), 849-860 (2004)
   PUBMED   15130477
REFERENCE   7  (bases 1 to 2060)
  AUTHORS   Monaghan,A.S., Benton,D.C., Bahia,P.K., Hosseini,R., Shah,Y.A.,
            Haylett,D.G. and Moss,G.W.
  TITLE     The SK3 subunit of small conductance Ca2+-activated K+ channels
            interacts with both SK1 and SK2 subunits in a heterologous
            expression system
  JOURNAL   J Biol Chem 279 (2), 1003-1009 (2004)
   PUBMED   14559917
REFERENCE   8  (bases 1 to 2060)
  AUTHORS   Schumacher,M.A., Rivard,A.F., Bachinger,H.P. and Adelman,J.P.
  TITLE     Structure of the gating domain of a Ca2+-activated K+ channel
            complexed with Ca2+/calmodulin
  JOURNAL   Nature 410 (6832), 1120-1124 (2001)
   PUBMED   11323678
REFERENCE   9  (bases 1 to 2060)
  AUTHORS   Xia,X.M., Fakler,B., Rivard,A., Wayman,G., Johnson-Pais,T.,
            Keen,J.E., Ishii,T., Hirschberg,B., Bond,C.T., Lutsenko,S.,
            Maylie,J. and Adelman,J.P.
  TITLE     Mechanism of calcium gating in small-conductance calcium-activated
            potassium channels
  JOURNAL   Nature 395 (6701), 503-507 (1998)
   PUBMED   9774106
REFERENCE   10 (bases 1 to 2060)
  AUTHORS   Kohler,M., Hirschberg,B., Bond,C.T., Kinzie,J.M., Marrion,N.V.,
            Maylie,J. and Adelman,J.P.
  TITLE     Small-conductance, calcium-activated potassium channels from
            mammalian brain
  JOURNAL   Science 273 (5282), 1709-1714 (1996)
   PUBMED   8781233
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000018.1.
            
            On Jun 2, 2015 this sequence version replaced NM_019314.1.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 3' coding region compared to variant 1. It encodes
            isoform 2 which is shorter than isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U69882.1, SRR26643298.73944.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-489               JAXUCZ010000018.1  40300927-40301415
            490-585             JAXUCZ010000018.1  40301999-40302094
            586-1004            JAXUCZ010000018.1  40338154-40338572
            1005-1146           JAXUCZ010000018.1  40411419-40411560
            1147-1257           JAXUCZ010000018.1  40428724-40428834
            1258-1385           JAXUCZ010000018.1  40436183-40436310
            1386-1455           JAXUCZ010000018.1  40442289-40442358
            1456-2060           JAXUCZ010000018.1  40444439-40445043
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="18"
                     /map="18q11"
     gene            1..2060
                     /gene="Kcnn2"
                     /gene_synonym="KCa2.2; SK2; SKCa 2; SKCa2"
                     /note="potassium calcium-activated channel subfamily N
                     member 2"
                     /db_xref="GeneID:54262"
                     /db_xref="RGD:2963"
     CDS             1..1743
                     /gene="Kcnn2"
                     /gene_synonym="KCa2.2; SK2; SKCa 2; SKCa2"
                     /note="isoform 2 is encoded by transcript variant 2; small
                     conductance calcium-activated potassium channel protein 2;
                     small conductance calcium-activated potassium channel
                     protein 2-like; potassium intermediate/small conductance
                     calcium-activated channel, subfamily N, member 2;
                     potassium channel, calcium activated intermediate/small
                     conductance subfamily N alpha, member 2"
                     /codon_start=1
                     /product="small conductance calcium-activated potassium
                     channel protein 2 isoform 2"
                     /protein_id="NP_062187.1"
                     /db_xref="GeneID:54262"
                     /db_xref="RGD:2963"
                     /translation="MSSCRYNGGVMRPLSNLSSSRRNLHEMDSEAQPLQPPASVVGGG
                     GGASSPSAAAAASSSAPEIVVSKPEHNNSNNLALYGTGGGGSTGGGGGGGGGGGGSGH
                     GSSSGTKSSKKKNQNIGYKLGHRRALFEKRKRLSDYALIFGMFGIVVMVIETELSWGA
                     YDKASLYSLALKCLISLSTIILLGLIIVYHAREIQLFMVDNGADDWRIAMTYERIFFI
                     CLEILVCAIHPIPGNYTFTWTARLAFSYAPSTTTADVDIILSIPMFLRLYLIARVMLL
                     HSKLFTDASSRSIGALNKINFNTRFVMKTLMTICPGTVLLVFSISLWIIAAWTVRACE
                     RYHDQQDVTSNFLGAMWLISITFLSIGYGDMVPNTYCGKGVCLLTGIMGAGCTALVVA
                     VVARKLELTKAEKHVHNFMMDTQLTKRVKNAAANVLRETWLIYKNTKLVKKIDHAKVR
                     KHQRKFLQAIHQLRSVKMEQRKLNDQANTLVDLAKTQNIMYDMISDLNERSEDFEKRI
                     VTLETKLETLIGSIHALPGLISQTIRQQQRDFIETQMENYDKHVTYNAERSRSSSRRR
                     RSSSTAPPTSSESS"
     exon            1..489
                     /gene="Kcnn2"
                     /gene_synonym="KCa2.2; SK2; SKCa 2; SKCa2"
                     /inference="alignment:Splign:2.1.0"
     exon            490..585
                     /gene="Kcnn2"
                     /gene_synonym="KCa2.2; SK2; SKCa 2; SKCa2"
                     /inference="alignment:Splign:2.1.0"
     exon            586..1004
                     /gene="Kcnn2"
                     /gene_synonym="KCa2.2; SK2; SKCa 2; SKCa2"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1146
                     /gene="Kcnn2"
                     /gene_synonym="KCa2.2; SK2; SKCa 2; SKCa2"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1257
                     /gene="Kcnn2"
                     /gene_synonym="KCa2.2; SK2; SKCa 2; SKCa2"
                     /inference="alignment:Splign:2.1.0"
     exon            1258..1385
                     /gene="Kcnn2"
                     /gene_synonym="KCa2.2; SK2; SKCa 2; SKCa2"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1455
                     /gene="Kcnn2"
                     /gene_synonym="KCa2.2; SK2; SKCa 2; SKCa2"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..2060
                     /gene="Kcnn2"
                     /gene_synonym="KCa2.2; SK2; SKCa 2; SKCa2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atgagcagct gcaggtacaa cgggggcgtc atgcgtccgc tcagcaactt gagctcgtcc
       61 cgccggaacc tgcacgagat ggactcagag gctcagcccc tgcagccccc agcgtcggtt
      121 gtaggaggag gtggtggtgc gtcctccccg tctgctgccg ccgccgcctc atcctcagcc
      181 ccagagatcg tggtgtctaa gccggagcac aacaattcta acaacctggc gctctacgga
      241 actggcggcg gaggcagcac cggaggcggc ggcggcggcg gcggcggcgg cggcggcagc
      301 gggcatggca gcagcagcgg cactaagtcc agcaaaaaga agaaccagaa catcggctat
      361 aagctgggcc atcggcgtgc cctgtttgag aagcgcaagc ggctcagcga ctatgcgctc
      421 atcttcggca tgttcggcat cgtggtcatg gtcatcgaga ccgagctgtc gtggggcgcc
      481 tacgacaagg cgtcgctgta ttctttagct ctgaaatgcc ttatcagtct ctccacgatc
      541 atcctgcttg gtctgatcat cgtataccac gccagggaaa tacagttatt catggtggac
      601 aatggagcag atgactggag aatagccatg acttatgagc gtattttctt catctgcttg
      661 gaaatactgg tgtgtgctat tcatcccatc cctgggaatt atacgttcac atggacagcc
      721 cggcttgcct tctcctatgc cccttccaca accactgcag acgtggatat tattttatct
      781 ataccaatgt tcttaagact ctatctgatt gccagagtca tgctattaca tagcaaactt
      841 ttcaccgatg cctcctctag aagcattggg gcacttaata agataaactt caatacgcgt
      901 tttgttatga agactttaat gactatctgc ccaggaactg tgctcttggt ttttagtatc
      961 tcgttatgga taattgccgc atggactgtc cgagcttgtg aaaggtacca tgatcaacag
     1021 gatgtcacta gcaacttcct tggagcaatg tggttgatat caataacttt tctctccatt
     1081 ggttatggtg acatggtacc taacacatac tgtgggaaag gagtctgctt gcttaccgga
     1141 ataatgggtg caggttgcac agccttggtg gtagccgtag tggcaagaaa gctagaactt
     1201 accaaagcag aaaagcatgt gcacaatttc atgatggata ctcagctgac caaaagagta
     1261 aaaaacgcag ccgccaatgt actcagggaa acgtggttaa tctacaaaaa cacaaagcta
     1321 gtgaaaaaga tcgaccatgc aaaagtaagg aagcatcaac ggaaattctt acaagctatt
     1381 catcaattaa gaagtgtgaa gatggaacag aggaaactga atgaccaagc gaatactcta
     1441 gtggatctgg caaagaccca gaatatcatg tatgatatga tttccgactt aaatgaaagg
     1501 agtgaagact ttgagaaaag gatcgtcacc ctggaaacaa aattagaaac tttgattggt
     1561 agcattcatg ccctccctgg gcttatcagc cagaccatca gacagcagca aagggacttc
     1621 atagagacac agatggagaa ctatgacaag catgtcacct acaatgctga gcgttcccgg
     1681 tcctcgtcca ggaggcggcg gtcctcctcc acagcgccac caacttcatc tgagagtagc
     1741 tagaagagaa taagttaacc acaaaataag actttttgcc atcatatggt caatatttta
     1801 gcttttattg taaagcccct atggttctaa tcagcgttat ccgggttctg atgtcagaat
     1861 catgggaacc tgaacactaa gttttaggcc aaaatatgag tgaaaactct ttttttttct
     1921 ttcagatgca cagggaatgc accaattatt gctatataga ttgttcctcc tgtaatttca
     1981 ctaacttttt attcatgcac ttcaaacaaa ctttactact acattatatg atatataata
     2041 aaaaagttaa tttctgcaca
//
